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1.
The objective of this study was to compare the clinical and laboratory consequences of left and right displacement of the abomasum (LDA and RDA), short- and long-term survival after surgery and the findings in cows, that could not be cured by omentopexy. Data from 564 cases of displaced abomasum (466 LDA, 98 RDA) were analysed retrospectively. Clinical and laboratory findings were compared between the two manifestations of DA. Survival was assessed after 10 days and after 15 months. Necropsy was carried out on cows that died or were killed. On arrival at the clinic, left displacement of the abomasum (LDA) cows had been recognized as diseased for longer. LDA occurred earlier in lactation, and more cows with right displacement of the abomasum (RDA) were pregnant. Overall clinical symptoms were more severe in RDA than in LDA cows. Heart rate was higher, body temperature was lower, inanition, abnormal faeces and ruminal stasis were more frequent in RDA cows. Leucocyte counts were higher, and potassium and chloride levels were lower in RDA cows. Acetonuria was more frequent in LDA cows. More LDA than RDA cows were released from the clinic as cured (82.0% versus 74.5%). However, survival after the early post-surgical period was similar for RDA and LDA cows. At necropsy, diseases of the gastrointestinal system were the predominant finding in RDA cows, while in LDA cows, diseases of the liver and other concurrent diseases were more important.  相似文献   
2.
A 4-year-old male German Hunting Terrier presented with tremor, dyspnea, trismus, spasms of the musculature of the larynx and pharynx, and hypothermia and subsequently died despite intensive clinical care. Prior clinical signs included vomitus and diarrhea. Microscopic examination of the brain revealed a multifocal nonsuppurative brain stem encephalitis; a few intralesional neurons contained intranuclear inclusions. By immunohistochemistry, Aujeszky disease virus (Suid herpesvirus 1) antigen was detected in neurons in the brain and in ganglion cells of the trigeminal ganglia. Viral culture of brain tissue confirmed the presence of Aujeszky disease virus. Histopathologic findings in the brain with the identification of Aujeszky disease virus by immunohistochemistry and polymerase chain reaction are consistent with Aujeszky disease virus-induced encephalitis. Sequencing revealed a 100% homology of the isolated Aujeszky disease virus with Aujeszky disease virus isolates of wild boar from Eastern Germany.  相似文献   
3.
Inhalation of bioaerosols from animal houses can induce acute inflammatory reactions in the respiratory tract. Determination of the concentration of airborne endotoxins is widely used to characterize this risk. In this study, the activity of bioaerosol samples from a duck-fattening unit to induce interleukin-1beta (IL-1beta) in human blood and to react with Limulus Amebocyte Lysate (LAL) was investigated. The activity detected in the whole blood assay correlated well with the endotoxic activity found in the LAL assay (Spearmen's rho = 0.902). However in all samples, the inflammation-inducing potential was overestimated by the LAL assay. It is assumed that this overestimation could be, in part, a result of an overestimation of the inflammatory potential of endotoxins originating from Pseudomonadaceae by the LAL assay. Pseudomonadaceae were regularly isolated from the air of the duck-fattening unit. The results presented here indicate that the whole blood assay can be used besides the LAL assay as an additional method to characterize the inflammation-inducing potential of bioaerosols.  相似文献   
4.
The objective of this study was to analyse the effect of dystocia on calf viability, milk production, fertility and culling under field (farm) conditions in a retrospective case-control study. A total of 253 calvings that were attended by a veterinarian were classified into three categories: mild dystocia (MD), severe dystocia (SD) and caesarean section (CS). For every case of dystocia, a control was chosen from the same farm and matched for calving month, breed and parity. Cases and controls were compared concerning calf viability, milk production, fertility and culling. The proportion of stillbirths was significantly higher in cows with dystocia compared with their controls. However, the difference was lower in CS cows than in the other two groups. MD had no significant effects on milk production, fertility and culling. There were no obvious effects of SD on monthly test day milk production, while CS cows produced significantly less milk than their controls. Compared with controls, less SD (50.0% versus 61.6%) and CS cows (48.6% versus 70.3%) conceived until 200 days in milk and more CS cows (31.1% versus 16.2%) were culled in the same time interval. These data demonstrate the scope of economic and animal welfare opportunities associated with the reduction of dystocia by management and breeding.  相似文献   
5.
The efficacy of a protocol for the synchronization of ovulation followed by timed Al (OVSYNCH) was compared with a reproductive management protocol based on induction of oestrus after rectal palpation using PGF2alpha on a commercial dairy farm in Brandenburg, Germany. Cows in the OVSYNCH group (n = 309) were treated between 62 and 68 days in milk (DIM) with 0.02 mg of buserelin (GnRH analogue) intramuscularly (i.m.). Seven days later 0.75 mg of tiaprost (PGF2alpha-analogue) were administered i.m. to regress the corpus luteum (C.I.). All cows received a second treatment with GnRH another 48 hrs later and were inseminated 16 to 20 hrs after the second GnRH-treatment (72 to 78 DIM). Cows in the PGF group (n = 302) were examined by rectal palpation between 69 and 75 DIM. Cows with a C.I. received 0.75 mg of tiaprost to induce oestrus. Cows were inseminated on observed oestrus. Cows not inseminated within 14 days after treatment were re-examined between 83 and 89 DIM. For both groups, the voluntary waiting period was set at 72 DIM. Service rate was higher (86.4 vs. 63.2 %, P < 0.05), days to first service (77 +/- 11 vs. 86 +/- 19 days, P < 0.001) and days open (102 +/- 34 vs. 109 +/- 35 days, P < 0.05) were shorter in the OVSYNCH group. First service conception rate (40.3 vs. 42.4 %), conception rate to all services (37.9 vs. 40.0 %) and the proportion of pregnant cows at 200 DIM were similar in the OVSYNCH evaluation for the total costs per pregnancy in 71 different cost scenarios showed only slight differences between the groups. Mean costs per pregnancy were euro 235.43 and euro 235.08 for the OVSYNCH and the PGF group, respectively. Quality of oestrus as assessed by the Al technician did not reveal a relationship to conception rate after OVSYNCH and timed Al. However, swelling, hyperaemia and a moist vestibulum were positively associated with conception rate.  相似文献   
6.
This study examines the association of pre-surgical clinical parameters in cows with left displacement of the abomasum (LDA) with the probability of cure. Data from 466 cases of LDA in which omentopexy was performed were analysed. Cows were classified in three categories according to the outcome of treatment. Cows in the first category were cured, i.e. sent back to the farm for further milk production. Cows in the second category were sent for slaughter because of an unfavourable prognosis concerning productivity. Cows in the third category either died or were salvaged because of poor prognosis concerning survival. Factors were examined for their possible influence on the outcome of treatment by comparing the values of successfully treated cows to those cows that either died or had to be sent for slaughter. Factors that were associated with a favourable prognosis were a short duration of disease, an undisturbed general condition, good appetite, normal faeces, a higher body weight, lower haematocrit, haemoglobin and erythrocyte counts, lower urea, AST and bilirubin, and higher sodium, potassium and chloride concentrations compared with cows with an unfavourable prognosis. Haemoconcentration was associated with higher AST, bilirubin and urea and with low sodium, potassium and chloride. Acetonuria was associated with increased AST and bilirubin and low urea. Low sodium and potassium concentrations were associated with an impaired general condition. It is concluded that a thorough clinical examination with special emphasis on general condition, liver function and dehydration status is of great importance in determining the prognosis of abdominal surgery in cows with LDA.  相似文献   
7.
In 2009, 1462 Escherichia coli isolates were collected in a systematic resistance monitoring approach from primary production, slaughterhouses and at retail and evaluated on the basis of epidemiological cut-off values. Besides resistance to antimicrobial classes that have been extensively used for a long time (e.g. sulphonamides and tetracyclines), resistance to (fluoro)quinolones and third-generation cephalosporins was observed. While in the poultry production chain the majority (60%) of isolates from laying hens was susceptible to all antimicrobials tested, most isolates from broilers, chicken meat and turkey meat showed resistance to at least one (85-93%) but frequently even to several antimicrobial classes (73-84%). In the cattle and pig production chain, the share of isolates showing resistance to at least one antimicrobial was lowest (16%) in dairy cows, whereas resistance to at least one antimicrobial ranged between 43% and 73% in veal calves, veal and pork. Resistance rates to ciprofloxacin and nalidixic acid in isolates from broilers were 41.1% and 43.1%, respectively. Likewise, high resistance rates to (fluoro)quinolones were observed in isolates from chicken meat and turkey meat. In contrast, ciprofloxacin resistance was less frequent in E.?coli isolates from the cattle and pig production chain with highest rate in veal calves (13.3%). Highest resistance rates to cephalosporins were observed in broilers and chicken meat, with 5.9% and 6.2% of the isolates showing resistance. In dairy cattle and veal, no isolates with cephalosporin resistance were detected, whereas 3.3% of the isolates from veal calves showed resistance to ceftazidime. Resistance to (fluoro)quinolones and cephalosporins in E.?coli isolates is of special concern because they are critically important antimicrobials in human antimicrobial therapy. The emergence of this resistance warrants increased monitoring. Together with continuous monitoring of antimicrobial usage, management strategies should be regularly assessed and adapted.  相似文献   
8.
The aim of this study was to examine influences of housing conditions on the udder health in 80 German dairy herds with a herd size between 100 and 1100 cows. Data were collected using a standardized questionnaire for the farm manager and a farm visit using a standardized data capture form on hygiene and management. The somatic cell counts of all lactating cows on each farm were collected monthly by the local dairy herd improvement association and analysed to assess udder health status. Factor analysis was used to analyse the variables describing the environmental hygiene. The values derived for the extracted components were classified into good, moderate and poor. The association of the categories was then analysed for their influence on log somatic cell count of the current month (CMSCC) and the year before the farm visit (YASCC) by a one-way anova. In comparison to other housing systems, free stalls with cubicles had the lowest geometric mean somatic cell count. Three components were derived from the factor analysis. Of those, acceptance of the cubicles by the cows and barn hygiene were determined as components influencing the CMSCC and YASCC significantly, while the association of hygiene of the milking parlour with somatic cell counts was only significant for YASCC. The results of the study show that the cow comfort and housing hygiene have a substantial impact on milk quality and should therefore become the focus of further research on the farm management practices.  相似文献   
9.
The objective of this study was to examine the influences of different milking practices on cow udder health in 80 large dairy herds (range 100-1100 cows) in Brandenburg, Germany. Milking practices were evaluated during one complete milking using a standardized data capture form. The somatic cell count (SCC) of all lactating cows on each farm was determined monthly by the local milk recording association 'Landeskontrollverband Brandenburg'. Factor analysis was used to investigate the relationship between the different aspects of the milking practices. The components extracted by the factor analysis were examined for their influence on the SCC of the current month (CMSCC) and the year before the visit (YASCC) using univariate analysis of variance. Three components were extracted from the milking practices. 'Reasonable use of water' was significantly related to CMSCC (P = 0.019) and YASCC (P = 0.003). It included information on the use of a hose to clean udders before milking, cleaning of the floor between groups and use of water to clean teats. 'Attention of the milkers' was also significantly associated with CMSCC (P = 0.012) and YASCC (P = 0.014). It included information on the accuracy of mastitis detection by foremilk screening and the regular use of post-milking teat and cluster disinfection. The component 'preparation routines' (method of udder cleaning and forestripping) did not significantly influence CMSCC and YASCC. These results indicate that excessive use of water in the parlour during milking time is harmful to udder health and that the consistency of procedures in the milking parlour presents significant room for improvement in large dairy herds in Brandenburg.  相似文献   
10.
Conception rates after Ovsynch have been higher in primiparous than in multiparous cows. The objective of this study was to investigate whether this difference might be due to differences in ovulation rate or follicular size. The experiment was conducted with 136 Holstein Frisian cows from a commercial herd in Brandenburg, Germany. All cows were synchronized using Buserelin (GnRH analogue) at day ?10, Tiaprost (PGF2α analogue) at day ?3 and again GnRH at day ?1. Timed artificial insemination (TAI) was carried out 16–20 h after the second dose of GnRH on day 0. Milk samples for analysis of milk progesterone were obtained on days ?17, ?10, ?3 and at TAI. Progesterone concentrations were used to determine the stage of oestrus cycle at the start of the synchronization protocol and to investigate the presence of functional luteal tissue before treatment with PGF2α and TAI. All animals were examined by ultrasound at the second treatment with GnRH, at AI, 8 and 24 h after AI. Overall synchronization rate (proportion of cows with an ovulation within 40 h after GnRH) was 86.8% in primiparous and 88.2% in multiparous cows, respectively. Ovulation occurred earlier in primparous than in multiparous cows (p < 0.05) and ovulatory follicles were smaller. Conception rates were numerically higher in primiparous cows but the difference was not significant. Cows that displayed signs of oestrus on day ?1 and received an additional AI on this day were more likely to conceive than cows that only received TAI 16 to 20 h after GnRH2. It is concluded that ovulation occurs earlier in primiparous than in multiparous cows after Ovsynch. However, a significant relationship between these differences and the probability of conception could not be established.  相似文献   
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