Immunocytochemical study of tissues from clinically normal dogs and of neoplasms, using keratin monoclonal antibodies.     

G E Sandusky  K A Wightman  W W Carlton 《American journal of veterinary research》1991,52(4):613-618

Three commonly used keratin monoclonal antibodies (MAB)--AE1:AE3, CAM 5.2, and MAK-6--were compared with routinely used cytokeratin antibody. The expression of these antibodies was analyzed in several tissues obtained from clinically normal dogs and in a variety of neoplasms from dogs. Using appropriate enzymatic digestion, paraffin-embedded tissues processed in routine manner retained their typical keratin expression. Differentiated and poorly differentiated epithelial neoplasms, lymphomas, and melanomas were studied by use of the avidin-biotin-peroxidase technique. All 4 of the aforementioned antibodies had similar staining profiles. Of 3 anaplastic carcinomas, 2 had positive reaction to all 4 antibodies. All lymphomas, plasma cell tumors, and amelanotic melanomas had negative reaction to MAK-6, CAM 5.2, AE1:AE3, and cytokeratin MAB. Three basal cell epitheliomas had positive reaction to all 4 antibodies, whereas 1 basal cell tumor with a solid pattern had negative staining reaction. Two carcinoids had negative reaction to all markers and 1 of 2 malignant chemodectomas and 1 transitional cell carcinoma had staining reaction to only AE1:AE3 MAB. Comparing the 4 antibodies, use of AE1:AE3 MAB produced the strongest staining intensity followed by cytokeratin, MAK-6, and CAM 5.2 MAB. All 4 antibodies had low background staining. In conclusion, AE1:AE3 and MAK-6 MAB are as useful as cytokeratin MAB for identification of poorly differentiated epithelial neoplasms in dogs and cats.  相似文献   

Presentation of postweaning Escherichia coli diarrhea in southern Ontario, prevalence of hemolytic E. coli serogroups involved, and their antimicrobial resistance patterns   总被引：1，自引：0，他引：1       下载免费PDF全文

Rocio Amezcua  Robert M. Friendship  Catherine E. Dewey  Carlton Gyles    John M. Fairbrother 《Canadian journal of veterinary research》2002,66(2):73-78

Post-weaning Escherichia coli diarrhea (PWECD) in Ontario was investigated using a case-control study involving 50 Ontario nurseries. The clinical signs and the impact on productive parameters were determined by means of a producer survey. The hemolytic E. coli serogroups involved in PWECD (O149:K91:K88) were examined in this study. Based on a polymerase chain reaction test, the hemolytic E. coli from 82% of the case herds were positive for 3 enterotoxins (STa, STb, and LT), those from 12% of the case herds were positive for STb and LT only, and those from one herd (6%) were positive for 3 enterotoxins, as well as for verotoxin and F18 pili. The E. coli involved in disease were resistant to multiple antibiotics. Case farms commonly used a wide variety of antibiotics either in the feed or water, or as injectable drugs. The most common antibiotic used to treat PWECD on the study farms was apramycin, but evidence of resistance to this antibiotic was noted. The PWECD problem was commonly seen within a week of weaning but onset of diarrhea was reported as late as the grower-finisher stage. Growth rate was poorer in case herds and mortality was higher than in control herds, demonstrating that PWECD is an economically important disease in Ontario.  相似文献   

Student debts and low wages     

Carlton Gyles 《The Canadian veterinary journal. La revue veterinaire canadienne》2015,56(12):1215-1216

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Growth of lucerne (Medicago sativa,L.) in response to frequency of irrigation and gypsum application on a heavy clay soil     

D. M. Whitfield  G. C. Wright  O. A. Gyles  A. J. Taylor 《Irrigation Science》1986,7(1):37-52

Summary Effects of weekly (W) and fortnightly (F) irrigation schedules on established stands of lucerne (Medicago sativa L.) grown on gypsum treated (G) and untreated (C) heavy clay soil were investigated. Two irrigations were applied under the fortnightly regime and four under the weekly schedule during a single cutting cycle. Growth and light interception were measured during both the vegetative and mature phases of growth.Leaf expansion, light interception and dry matter production were greater under treatments W _G and W _C, with yield increasing from 3.4 t ha^–1 under the fortnightly schedule to 5.0 t ha-1 with weekly irrigation. Gypsum treatment was effective under the more frequent irrigation schedule. Specific leaf area and the proportion of stem were both increased by treatments W _G and W _C. The responses to irrigation were therefore characteristic of those elicited by a more favourable plant moisture status. Growth was analysed in terms of light interception, the efficiency of utilisation of intercepted light, the proportion of the daily dry matter gain retained by the leaves and leaf expansion. The analysis demonstrated that impaired leaf expansion contributed to a decline of approximately 15% in yield, and that impaired efficiencies of utilisation of intercepted light contributed to losses of approximately 30% under the less frequent irrigation schedule.A comparison of growth rates and efficiencies of energy conversion with published data showed that satisfactory rates of growth and levels of productivity were achieved on the heavy soils of the local region using gypsum treatment and the more frequent irrigation schedule.  相似文献   

Accelerating invasion rate in a highly invaded estuary   总被引：4，自引：0，他引：4  

AN Cohen  JT Carlton 《Science (New York, N.Y.)》1998,279(5350):555-558

Biological invasions are a major global environmental and economic problem. Analysis of the San Francisco Bay and Delta ecosystem revealed a large number of exotic species that dominate many habitats in terms of number of species, number of individuals and biomass, and a high and accelerating rate of invasion. These factors suggest that this may be the most invaded estuary in the world. Possible causes include a large number and variety of transport vectors, a depauperate native biota, and extensive natural and anthropogenic disturbance.  相似文献   

Tagging and elimination of plasmids in Salmonella of avian origin   总被引：2，自引：0，他引：2  

C Poppe  C L Gyles 《Veterinary microbiology》1988,18(1):73-87

This study compared the effectiveness of a number of procedures designed to label and eliminate plasmids that may play a role in virulence in Salmonella. Twenty strains of Salmonella of 9 serovars were subjected to 3 methods for labelling plasmids with transposons. Strains containing labelled and unlabelled plasmids were exposed to physical and chemical curing agents. Plasmids in 9 of 20 strains of Salmonella were tagged by conjugation with a donor Escherichia coli containing a temperature-sensitive RP4 plasmid that carried the Tn1 transposon. Plasmids in 2 of 5 strains of Salmonella were labelled by conjugation with a donor E. coli that contained a F' tslac::Tn5 plasmid. Transduction of Salmonella with a P22 bacteriophage that carried a temperature-sensitive Tn10 transposon resulted in chromosomal insertion of Tn10 in 2 of 10 strains. Use of chemical curing agents resulted in curing of plasmids in only 6 of 17 strains. Two strains were cured by ethidium bromide, two by a combination of ethidium bromide and novobiocin, two by a combination of imipramine and methylene blue, and none by acridine orange, novobiocin, sodium dodecyl sulfate or rifampicin. In contrast, plasmids in 14 of 17 Salmonella strains were eliminated by incubation at 45.5 degrees C.  相似文献   

Mycotoxicosis produced in swine by cultural products of an isolate of Aspergillus ochraceus. II. Clinicopathologic changes   总被引：1，自引：0，他引：1  

J L Zimmermann  W W Carlton  J Tuite 《Veterinary pathology》1979,16(6):702-709

Rice culture of a toxigenic strain of Aspergillus ochraceus was fed at a concentration of 25% to weanling pigs for 10 days. The clinicopathological abnormalities reflected renal damage. Activities of lactic dehydrogenase, glutamic-oxaloacetic transaminase and isocitrate dehydrogenase were increased in the urine but not in the serum. Serum concentrations of urea nitrogen and creatinine were high. Cellular and granular casts, blood, protein, and glucose were in the urine of pigs fed toxic diet. Serum concentrations of K+, Na+ and Cl- were unchanged, but concentrations of these electrolytes were reduced in the urine.  相似文献   

Ochratoxicosis in Beagle dogs. II. Pathology   总被引：2，自引：0，他引：2  

G M Szczech  W W Carlton  J Tuite 《Veterinary pathology》1973,10(3):219-231

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Ochratoxicosis in Beagle dogs. I. Clinical and clinicopathological features   总被引：3，自引：0，他引：3  

G M Szczech  W W Carlton  J Tuite 《Veterinary pathology》1973,10(2):135-154

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Experimental aflatoxicosis in young swine   总被引：1，自引：0，他引：1  

D B Sisk  W W Carlton  T M Curtin 《American journal of veterinary research》1968,29(8):1591-1602

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