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Marco A. Lima DVM Carmen Calles DVM Juan R. Lima DVM Maria F. Martin PD PhD Veronica Crisostomo DVM Francisco J. Hernandez MD PhD Rafael Barrera DVM PhD Jesus Uson DVM PhD 《Veterinary radiology & ultrasound》2002,43(2):171-177
The aim of this study was to use ultrasonography to assess an experimental carotid arteriotomy followed by end-to-end anastomosis in the lamb. Animals were subjected to an experimental surgical procedure consisting of a right carotid artery arteriotomy and subsequent end-to-end anastomosis with nonpenetrating metallic clips. Left side arteries were used as control. Duplex ultrasonography was performed postoperatively to assess the evolution of the experimental anastomosis. Measurements were made on the right carotid to assess blood flow, peak systolic velocity, and end diastolic velocity, as well as arterial diameter. Animals were periodically checked during the six-month postoperative period. Operated vessels appeared normal during follow up, with pulsatile movements and size similar to non operated carotid arteries. A hyperechoic band, perpendicular to the vessel main axis, was seen on the longitudinal image in operated arteries, but not in the control group. This band corresponded to the anastomosis area, where the clips were positioned. No image suggestive of thrombosis was observed in any operated artery, and blood flow was maintained through the experiment. Finally, a decrease of vessel lumen diameter was evidenced at the level of the anastomosis during the first 30 postoperative days, but it was not present after the 90th postsurgical day. Duplex ultrasonography is a useful imaging technique for the assessment of anatomical details, acoustical properties of the vascular lumen, and study of the intimal surface and vessel wall in growing arteries subjected to surgery. Metallic clips as suturing technique in arterial anastomosis allow for normal vascular growth, as shown by the evolution of flow velocity and vessel diameter. 相似文献
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Mariella Matilde Finetti-Sialer Tiziana Mascia Fabrizio Cillo Crisostomo Vovlas Donato Gallitelli 《European journal of plant pathology / European Foundation for Plant Pathology》2012,132(3):317-322
The biological and molecular characterization is reported of a Watermelon mosaic virus isolate, denoted WMV-Le, associated with a necrotic phenotype of watermelon plants grown in the Provinces of Lecce and Taranto
(Apulia, southern Italy). The fully sequenced WMV-Le genome consists of 10,045 nucleotides and is 99.1% similar to that of
WMV-C05-270, a French isolate from melon of the WMV molecular group 3. Using recombination detection program RDP3, putative
recombination breakpoints were identified close to nucleotide positions 42 to 1892, covering the 5′UTR/P1/HC-Pro region. The
event represents the insertion of a sequence fragment of an isolate similar to WMV-FBR04-37 in the background of an isolate
similar to WMV-FMF00-LL1. The field symptomatology was reproduced in watermelon plants grown in an experimental greenhouse
but the virus induced severe symptoms also in Cucumis sativus, C. melo, Cucurbita maxima and C. pepo . 相似文献
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