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R. Iori O. Leoni L. Lazzeri G. Mosca S. Palmieri 《Journal of Agronomy and Crop Science》1991,167(2):91-95
Samples of rapeseed from three Italian growing environments (Bologna, Perugia and Palermo) were analysed for glucose content and dry weight of 1000 seeds every three or four days starting from the end of flowering until complete ripening. In addition, the content of oil, soluble and total proteins, glucosinolates and myrosinase activity was determined in samples of mature seeds. The cultivars used were jet Neuf and Lingot (type 0) and Tandem, Jade and Santana (type 00). From the results it emerged that the point of intersection of the two branches of the linear regression plots for different glucose-consumption kinetics found during seed filling, in addition to being strongly affected by the climate of the test environment, is correlated with quantitative and qualitative production, independently of the genotype. 相似文献
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A Leoni A M Nieddu F Guarda M Castagnaro A Firinu P Cossu V Mingioni 《Schweizer Archiv für Tierheilkunde》1989,131(6):361-368
A severe neurological disorder was observed during a very dry season, in sheep pasturing on land with mediterranean vegetation. During the crisis, lasting 5-20", ataxia and convulsions were the main clinical signs. Brain congestion, spongy appearance of the liver, interstitial nephritis and hyperpigmentation of visceral lymph nodes were the most important macroscopic lesions. Histopathological, histochemical and ultrastructural findings confirmed liver and kidney lesions and showed considerable neurovisceral storage of lipofuscin. Although further toxicological and experimental studies are needed to elucidate the origin of the disease, the Authors attribute the outbreak of the neurological syndrome to the ingestion of a neurotoxin containing plant. 相似文献
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Three feeding methods were compared in 36 4- to 6-year-old Franche-Montagne horses during the military school of St-Luzisteig (GR) of Spring 1992. The horses were separated into 3 groups: a group with the traditional oats-hay ration (OH), a group with a pelleted feed and hay ration (PFH), and a group with the complete diet (CD). Feed analyses were performed and food consumption, eating behavior and digestibility were studied. The horses received their daily amount of feed in 3 portions covering the requirements for a medium work: OH = 8 kg hay and 3 kg oats, PFH = 8 kg hay and 3 kg pelleted feed and CD = 10 kg of the complete diet. For the 3 rations, the amount of digestible crude protein for horses was higher than the reference value for the requirement of a 600 kg horse with a medium work. In the 3 diets, the calcium content was higher than the required 32 g per day (g/d). Not enough sodium (OH: 1.2 g/d; PFH: 7.3 g/d; CD: 9.6 g/d) and too much potassium (OH: 140.3 g/d; PFH 153.0 g/d; CD: 167.5 g/d) were present in the diets, both without consequences for the blood parameters. In 3 meals of 60 minutes, the horses of the group OH, PFH and CD ingested 82%, 89% and 92%, respectively, of the daily ration. The complete diet was ingested more quickly than the hay. The number of mastications per minute was smaller for the complete diet than for the hay. Ingestion times were similar for oats and pelleted feed. However, the number of mastications per minute was smaller for the pelleted feed. The digestibility of nutrients was not influenced by the method of feeding. In conclusion, these results demonstrate that the 3 types of ration studied here are adequate for the swiss army horses if sodium is added to the diet. However, despite the fact that both PFH and CD correct excessive supply or deficiencies of nutrients and despite the fact that these two feeding methods offer nutrients in amounts that are closer to the requirements of the horse, the method PFH was introduced in 1994. 相似文献
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V. Parreo V. Costantini S. Cheetham J. Blanco Viera L. J. Saif F. Fernndez L. Leoni A. Schudel 《Zoonoses and public health》2001,48(9):713-720
Group A rotavirus (RV) and coronavirus (CV) are common viral pathogens associated with neonatal diarrhoea in numerous animal species. The purpose of this work was to investigate the presence of these viral agents in two farm populations of captured guanacos (Lama guanicoe) in the Argentinean Patagonia region, that developed severe diarrhoea outbreaks. Stool and serum samples were analysed for RV and bovine CV antigen and antibody by enzyme‐linked immunosorbent assay. Rotavirus was detected in faeces from two new‐born guanacos with acute diarrhoea, one in each farm. After electrophoretic analysis, each isolated strain, showed a distinctive long dsRNA electropherotype characteristic of group A rotaviruses (4:2:3:2). In addition, 95% (38 of 40) of the sampled animals were positive for RV antibodies, suggesting a high prevalence of RV infection in the populations tested. No evidence of CV circulation by antigen or antibody analysis was observed. To our knowledge, this is the first report of the detection and isolation of RV associated with neonatal diarrhoea in Lama guanicoe. 相似文献
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Crambe abyssinica is becoming a reliable source of industrial oil with good technical characteristics because of its high erucic acid content. The possibility of using the industrial defatted meal of crambe as a feed could further promote the interest in this crop, but is prevented by the high glucosinolate content (70‐150 μmol/g). Therefore the possibility of reducing this parameter by selection was evaluated and a search for a new quick analytical procedure begun. When hydrolysed by myrosinase (thioglucoside glucohydrolase EC 3.2.3.1.), at pH 6.5 or higher, epiprogoitrin, the main glucosinolate in crambe seed (>90% of total glucosinolates), gives (5R)‐5‐vinyl‐1,3‐oxazolidine‐2‐thione quantitatively. The latter can be easily estimated by high‐performance liquid chromatography. This is the background of the proposed analytical procedure that permits rapid analysis of about 100 samples per day using about 25 mg of crambe meal (five seeds) for assay. The method can also be used to select for glucosinolate content in rapeseed and, probably with some adjustment, all crops that have a 2‐hydroxy‐glucosinolate as the main glucosinolate. 相似文献
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Manunta ML Gavini E Chessa G Passino ES Careddu GM Giua S Mollica A Demontis MP Leoni A Muzzetto P 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2005,52(8):416-422
A controlled carboplatin delivery system using biodegradable polymer has been used in this study. The purpose was to evaluate the local and systemic effects of injectable, biodegradable microspheres containing carboplatin when injected as aqueous suspension subcutaneously in rats. Biocompatibility and toxicity of empty microspheres and microspheres loaded with carboplatin were evaluated by clinical and histological examination. The diffusion of carboplatin in tissues and time of drug release were evaluated by platinum determination in plasma and tissues over the time. The results of the study suggest that microspheres provide a sustained slow release of carboplatin and that multiple inoculations of microspheres containing drug and no evidence of local or systemic toxicity is found. This device may be useful in the treatment of solid tumours. 相似文献
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Luvoni GC Kalchschmidt E Leoni S Ruggiero C 《Journal of Feline Medicine and Surgery》2003,5(4):203-208
There has been increased interest recently in the conservation of wild felids and preservation of valuable cat breeds. Assisted reproduction, by means of artificial insemination (AI), is an important tool for developing breeding programs for conservation. Optimal use of AI requires accurate data on semen conservation protocols and its long-term storage/survival. In this paper, semen cooling and freezing processes are described, with special emphasis on the results obtained in experiments performed in the domestic cat. Conception rates after AI in wild and domestic cats are also reported. 相似文献
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Luisa M Manici Onofrio Leoni Luca Lazzeri Stefania Galletti Sandro Palmieri 《Pest management science》1999,55(4):486-488
Enzyme-derived products obtained from thio-functionalised glucosinolates showed high fungitoxicity, a wide activity spectrum and special physicochemical properties, which suggest their potential as alternatives to commercial fumigants for controlling several soil-borne pathogens. © 1999 Society of Chemical Industry 相似文献