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This paper presents an epidemiological investigation of Ontario Ministry of Natural Resources Fish Health Laboratory data from 1981 to 1997, to determine whether fish species and age were associated with lot-level detection of Aeromonas salmonicida and Yersinia ruckeri in hatchery fish. In stepwise logistic regression, the species brook trout and back-cross (lake trout crossed with the hybrid “splake”) were more likely to test A. salmonicida-positive compared to all other species reared in the hatcheries. Similarly, the species brook trout was significantly more likely to test Y. ruckeri-positive compared to all other species. For both pathogens, the 1–5-month age group was associated significantly with detection. These findings suggest that purposive sampling of higher-risk fish lots could increase the likelihood of detecting both study pathogens. 相似文献
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锥栗干制研究Ⅰ.锥栗鲜果中坏粒剔除研究 总被引:3,自引:0,他引:3
对锥栗干制品制作中所产生的原料坏粒剔除进行了研究。实验结果表明 ,通过对原料抽样比较 ,测定坏果率的含量 ,得出最佳抽样数量 ,使样品的检测结果具有代表性 ;通过对个体坏果比重的测定 ,得出总体坏果的比重 ,能最大限度剔除坏果 ;通过盐水浓度的选择 ,剔除鲜果中的坏果 ,从而保证了成品的质量。 相似文献
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Orla M Keane Jason D Keegan Barbara Good Theo de Waal June Fanning Michael Gottstein Mícheál Casey Christine Hurley Maresa Sheehan 《Irish veterinary journal》2014,67(1):16
Background
In 2013 a Technology Adoption Program for sheep farmers was established to encourage the implementation of best management practices on sheep farms in Ireland. There were 4,500 participants in this programme in 2013. As part of this programme, farmers had the option to carry out a drench test to establish the efficacy of their anthelmintic treatment.Results
Flock faecal samples were collected before and after treatment administration and gastrointestinal nematode eggs enumerated. In total there were 1,893 participants in the task, however only 1,585 included both a pre- and post-treatment faecal sample. Of those, 1,308 provided information on the anthelmintic product that they used with 46%, 23% and 28% using a benzimidazole (BZ), levamisole (LEV) and macrocyclic lactone (ML) product respectively. The remaining farmers used a product inapplicable for inclusion in the task such as a flukicide or BZ/LEV combination product. Samples were included for analysis of drench efficacy if the pre-treatment flock egg count was ≥200 eggs per gram and the interval post-sampling was 10–14 days for BZ products, 4–7 days for LEV products and 14–18 days for ML products. These criteria reduced the number of valid tests to 369, 19.5% of all tests conducted. If the reduction post-treatment was ≥95% the treatment was considered effective. Only 51% of treatments were considered effective using this criterion. There was a significant difference in efficacy between the anthelmintic drug classes with BZ effective in only 30% of treatments, LEV effective in 52% of cases and ML effective in 76% of cases.Conclusions
Gastrointestinal nematode anthelmintic treatments, as practiced on Irish farms, have a high failure rate. There was a significant difference between the efficacies of the anthelmintic classes with BZ the least effective and ML the most effective. 相似文献7.
John Gordon Bell Fiona Strachan Joanne E Good & Douglas R Tocher 《Aquaculture Research》2006,37(6):606-617
Echium oil (EO) is a vegetable oil in which percentages of stearidonic acid (STA, 18:4n‐3) often exceed those of its n‐6 series equivalent γ‐linolenic acid (GLA, 18:3n‐6). Stearidonic acid is elongated to 20:4n‐3 in fish cell cultures, suggesting that EO could be included in diets for marine fish to increase tissue 20:4n‐3 and 20:3n‐6 and, thereby, modulate eicosanoid metabolism. Thus, the present study aimed to test the hypotheses that dietary EO would increase tissue 20:4n‐3 and 20:3n‐6 and modulate immune function and eicosanoid production in juvenile Atlantic cod (Gadus morhua L.) fed a diet where fish oil (FO) was replaced by EO. Duplicate groups of juvenile cod (initial weight ca. 4 g) were fed for 18 weeks on fish meal‐based diets (55% protein and 16% lipid) that differed in oil source (FO or EO). There were no significant differences in growth and feed efficiency, hepato‐somatic index, percentages of liver and flesh lipids and lipid class compositions for cod fed FO and EO. Percentages of 18:4n‐3, 18:3n‐6 and 20:3n‐6 in the total lipids of flesh and liver were higher, and percentages of 20:5n‐3 and 20:4n‐6 were both lower in fish fed EO than in those given FO. In flesh, the increased 18:3n‐6 and 18:4n‐3 were primarily located in phosphatidylcholine and, to a lesser extent, phosphatidylethanolamine, whereas 20:3n‐6 concentration was highest in phosphatidylinositol. Desaturation of 18:3n‐3 (to tetraene products) and 20:5n‐3 to 22:6n‐3 in hepatocytes was very low but was increased by dietary EO. Echium oil significantly decreased the production of prostaglandin F from gill cells stimulated with calcium ionophore A23187, and reduced head kidney macrophage activity, but had no effect on serum lysozyme activity or basic haematology. In conclusion, dietary EO may have beneficial effects on some immune parameters including eicosanoid metabolism in marine fish although this may be primarily because of decreased 20:4n‐6 rather than increasing tissue levels of 20:3n‐6 or 20:4n‐3. 相似文献
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Supernatants from cultures of normal feline lymphocytes stimulated with Staphylococcus enterotoxin A showed antiviral activity, characterized as a gamma-like interferon. With the addition of inactivated feline leukemia virus, markedly less interferon was produced. The reduction in interferon production was not attributable to lowered lymphocyte viability or reduced mitogenic properties of Staphylococcus enterotoxin A and appears to be a direct retroviral effect. This finding may reflect clinically relevant events that may contribute to the development of the feline or human states of acquired immunodeficiency. 相似文献
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White Leghorn chickens were subjected to bursectomy and total body irradiation at hatching, thus rendering them agammaglobulinemic. At 4 weeks of age, these chickens and control chickens received lyophilized bovine spinal cord emulsified with complete Freund's adjuvant. Agammaglobulinemic chickens developed the lesions of allergic encephalomyelitis with the same frequency and intensity as the controls. Our findings support the contention that antibodies are not necessary for the development of experimental allergic encephalomyelitis. 相似文献
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Gormley E Doyle MB McGill K Costello E Good M Collins JD 《Veterinary immunology and immunopathology》2004,102(4):413-420
The strategic use of the gamma-interferon (IFN-gamma) assay (Bovigam) can provide a means for the early identification of Mycobacterium bovis infected cattle, thus ensuring their removal from an infected herd. It has been reported that performance of the test can be influenced by various factors including a recent tuberculin skin test and the length of delay between collection and processing of blood samples. In this study, single intradermal comparative tuberculin test (SICTT) reactor and non-reactor cattle were recruited from herds infected with M. bovis and grouped according to their SICTT responses. Group 1 comprised reactor cattle selected on the basis of their SICTT response to PPD-bovine (purified protein derivative of tuberculin) exceeding that of PPD-avian by at least 12mm. Group 2 animals were selected from herds undergoing routine surveillance for bovine tuberculosis and contained standard SICTT reactor cattle (PPD-bovine exceeding that of PPD-avian by at least 4mm) and non-reactors. We investigated the effects of the SICTT on the assay results by measuring the in vitro IFN-gamma responses of Group 1 reactor cattle at time intervals pre- and post-skin test. No significant differences were measured in the IFN-gamma responses of the reactor animals to PPD-bovine and PPD-avian for up to 65 days. To investigate if a delay in processing of blood affected the performance of the assay, we compared results using duplicate blood samples from Group 1 and Group 2 cattle stimulated with PPD antigen at 8h and at 24h after collection. In both groups of animals the mean optical density (OD) values of the assay at 24h post-collection were significantly lower than those at 8h. Our results demonstrated that a delay in processing of the blood samples from cattle subjected to routine surveillance could significantly impact on the outcome of the IFN-gamma assay resulting in a change of the IFN-gamma status of the animals. 相似文献