Utilization of digital differential display to identify differentially expressed genes related to rumen development          下载免费PDF全文

Daichi Kato  Yutaka Suzuki  Satoshi Haga  KyoungHa So  Eri Yamauchi  Miwa Nakano  Hiroshi Ishizaki  Kichoon Choi  Kazuo Katoh  Sang‐Gun Roh 《Animal Science Journal》2016,87(4):584-590

This study aimed to identify the genes associated with the development of the rumen epithelium by screening for candidate genes by digital differential display (DDD) in silico. Using DDD in NCBI's UniGene database, expressed sequence tag (EST)‐based gene expression profiles were analyzed in rumen, reticulum, omasum, abomasum and other tissues in cattle. One hundred and ten candidate genes with high expression in the rumen were derived from a library of all tissues. The expression levels of 11 genes in all candidate genes were analyzed in the rumen, reticulum, omasum and abomasum of nine Japanese Black male calves (5‐week‐old pre‐weaning: n = 3; 15‐week‐old weaned calves: n = 6). Among the 11 genes, only 3‐hydroxy‐3‐methylglutaryl‐CoA synthase 2 (HMGCS2), aldo‐keto reductase family 1, member C1‐like (AKR1C1), and fatty acid binding protein 3 (FABP3) showed significant changes in the levels of gene expression in the rumen between the pre‐ and post‐weaning of calves. These results indicate that DDD analysis in silico can be useful for screening candidate genes related to rumen development, and that the changes in expression levels of three genes in the rumen may have been caused by weaning, aging or both. © 2015 Japanese Society of Animal Science  相似文献   

Glutathione content of in vivo and in vitro matured canine oocytes collected from different reproductive stages   总被引：3，自引：0，他引：3  

Kim MK  Hossein MS  Oh HJ  Fibrianto HY  Jang G  Kim HJ  Hong SG  Park JE  Kang SK  Lee BC 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(6):627-632

Glutathione (GSH) concentrations of oocytes are considered as an important marker of the cytoplasmic maturation. The present study was designed to compare GSH concentrations of in vivo and in vitro matured canine oocytes. In vivo matured oocytes were collected 72 hr after ovulation by flushing fallopian tubes after laparotomy. Ovaries were collected from bitches with different reproductive stages, and collected oocytes were divided into 2 groups according to the size viz. < 120 microm and > 120 microm in diameter and cultured for 72 hr in Tissue Culture Medium-199 supplemented with 10% FBS, 2.2 mg/ml sodium bicarbonate, 2.0 microg/ml estrogen, 0.5 microg/ml FSH, 0.03 IU/ml hCG, and 1% penicillin-streptomycin solution in the presence or absence of 50 microM beta-mercaptoethanol. GSH concentrations were determined by the dithionitrobenzoic acid-glutathione disulfide (DTNB-GSSG) reductase recycling assay. GSH concentrations of immature canine oocytes were 2.9 and 3.8, 3.5 and 6.8, and 3.1 and 6.5 pM/oocyte for < 120 microm and > 120 microm in diameter oocyte groups at anestrous, follicular and luteal stage, respectively (P<0.05). In vivo matured oocytes had significantly higher GSH concentrations compared with in vitro matured oocytes. The GSH content was 19.2 pM/oocyte for in vivo matured oocytes, while 4.1 to 8.1 and 5.7 to 13.2 pM/oocyte for in vitro matured oocytes cultured in the absence or presence of beta-mercaptoethanol, respectively (P<0.05). Presence of beta-mercaptoethanol increased GSH synthesis in canine oocytes cultured in vitro, and oocytes collected from follicular and luteal stage was superior to anestrus oocytes.  相似文献   

集对分析法在区域雨水资源开发利用潜力综合评价中的应用   总被引：4，自引：0，他引：4  

郭文献  徐建新  卢双宝 《灌溉排水学报》2005,24(4):66-68,72

探讨了区域雨水资源开发利用潜力的综合评价方法,并将集对分析理论应用于该研究,建立了区域雨水资源开发利用潜力综合评价模型。实例分析表明,该方法用于评价区域雨水资源开发利用潜力,方法简单,评价结果符合实际情况。  相似文献   

白背飞虱爆发规律及防治研究   总被引：4，自引：0，他引：4  

肖英方  陈海新 《西南大学学报(自然科学版)》1998,20(5):460-464

】江淮稻区白背飞虱呈迁入代至主害代的爆发型相,发生程度为基数决定型。根据白背飞虱历年田间系统调查资料和灯下虫量的逐步回归分析,证明江淮稻区主害代（２代）发生程度与迁入峰后７月１５日白背飞虱总虫量显著相关（Ｒ２＝０．７１０７）,并分别建立白背飞虱预警指标。以为害损失５％以下为标准,主害代高峰期虫量为１５头／穴,比原防治指标稍宽。由此提出病虫兼治的防治对策和技术规范,比原传统方法少用药１次。  相似文献   

'合作猪'TDRP基因的克隆及生物信息学分析     

蒋应弟  滚双宝  袁慧芳  曾国敏  高景波 《甘肃农业大学学报》2017,52(4)

[目的]丰富猪TDRP1基因研究的基础数据.[方法]以猪NM_001198925序列为参考,设计特异性引物,通过克隆测序获得'合作猪'TDRP1基因完整CDS序列,并进行生物信息学分析,同时采用实时荧光定量PCR方法检测TDRP1基因在不同组织中的表达特性.[结果]获得了TDRP1基因完整的CDS序列(GenBank登录号:KU743254),共684bp,其编码186个氨基酸多肽.与参考序列相比,在编码区第33、348位点处发生了同义突变(C→G、C→T).TDRP1基因分子式为C897H1421N259O287S2,理论等电点(PI)为5.86,不稳定系数为62.66,疏水指数为64.03,平均亲水性为-0.939,属不稳定可溶性酸性蛋白质.二级结构以无规则卷曲和α-螺旋为主,属混合类蛋白质.亚细胞定位结果显示,TDRP1编码的蛋白质在遗传物质复制和转录、翻译过程中发挥功能的可能性分别为26.3%、14.3%,明显高于发挥其它功能的可能性.mRNA表达分析表明,TDRP1基因在垂体和睾丸中高表达,肺、肾、小脑、卵巢中度表达,肝、脾、大脑、胃、小肠中低表达,心脏组织中不表达.[结论]成功克隆了'合作猪'TDRP1基因的完整CDS区序列,并发现了2个SNP位点;多组织转录表达分析表明TDRP1在垂体和睾丸中表达较高,可为深入研究TDRP1基因的功能提供参考.  相似文献   

高繁殖力猪新品系-HN肥育性能、胴体品质与年生产力研究     

李生贵  王鹏飞  张生伟  黄旺洲  张昌吉  滚双宝  马艳萍  黄文东  魏万鹏 《甘肃农业大学学报》2017,52(5):13-19

【目的】研究高繁殖力猪新品系-HN的肥育性能、胴体品质及年生产力.【方法】以大白猪为对照,测定了高繁殖力猪新品系-HN 2.5~6.5月龄肥育性能及活体质量60,70,80,90kg时的胴体性能和肉质,分析其经济效益及年生产力水平.【结果】高繁殖力猪新品系-HN全期平均日增质量为614.00g/d,极显著低于大白猪(P0.01),3.5~4.5月龄时生长速度最快,日增质量达774.19g/d,全期料肉比为3.19,高于大白猪;各个体质量阶段屠宰率均在68%左右,胴体瘦肉率在51%~56%;90kg屠宰时胴体质量、眼肌面积最大,分别是61.48kg和40.73cm2,80kg屠宰时后腿比例、净肉率最大,分别是29.34%和73.12%,肉质指标都在正常范围内;新品系-HN每头母猪年提供育肥仔猪比大白猪多10.16头,60,70,80,90kg屠宰时,母猪年生产力(产肉量)分别为1 107.25,1 282.98,1 461.98,1 644.24kg.根据当前市场价格,各体质量阶段育肥猪出售时,母猪年纯盈利分别为3 681.34,5 415.34,6 335.50,6 497.25元,综合经济效益高于100kg大白猪.【结论】高繁殖力猪新品系-HN肥育性能好,胴体品质优良,综合考虑肥育性能和经济效益,80kg出售或屠宰,经济效益最好.  相似文献   

《名特优绿茶初加工技术研究及示范项目》实施方法与成效初报     

郭雅玲  黄碧玉  赖凌凌  沈承武  张宝秀  刘旋  林健 《福建茶叶》2007,(1):10-12

本文对《名特优绿茶初加工技术研究及示范》项目的实施方法与初步成效进行了回顾与总结,归纳提出主要的实施技术方法与途径,排查疑难,增强信心;开展调研,加强交流;规范工艺,控制质量;引进设备,改良产品;产品创优,规模发展,为提高优质率寻求技术途径。  相似文献   

Forest condition and management in Swedish forest commons     

Eva Holmgren  Gun Lidestav  Göran Kempe 《Small-Scale Forestry》2004,3(3):453-468

Forest commons are regarded as a means to support local development and sustainable forest conditions. To evaluate the development impact of Swedish forest commons, comparative surveys have been undertaken in three regions, and the differences in forest condition and management between categories of commons as well as their relation to other forest ownerships have been assessed. Regional differences between the by-laws, historical development and geographical conditions are apparent. It is concluded that two of three regions have an overly restrictive harvesting policy given the purpose of the forest commons and the official forest policy. The study results underline the importance of evaluation of the performance of forest management in relation to management objectives, to ownership alternatives and to the impact of local variations in preconditions.  相似文献   

树皮人造板及相关建筑材料的制备和应用     

袁东  王晓军  兰拓  曹旗  张双保 《林产工业》2004,31(3):7-9

介绍了利用木质树皮制备人造板及相关建筑材料的生产工艺技术及其产品的特性,提出了木质树皮板的应用前景。  相似文献   

一株猪源发酵乳杆菌的体外特性研究     

李舒宁  滚双宝  季海峰  张董燕  王晶  王四新  刘辉 《甘肃农业大学学报》2014,(5):15-20

采用全自动曲线分析仪测定了猪源发酵乳杆菌X6的不同接种量、不同初始pH的生长曲线;采用琼脂平板扩散法检测该菌的抑菌能力,探讨该菌在不同高温下的存活时间;在体外模拟了该菌对人工胃液和人工肠液的耐受性.结果表明:在接种量1%、初始pH 5.3的条件下,该菌的生长情况最佳;该菌能够抑制大肠杆菌、金黄色葡萄球菌和沙门氏菌的生长;该菌在50、55、60℃处理2min时,存活率分别为99.19%、91.94%、80.65%;该菌在人工肠液和pH 3.0的人工胃液中存在4h后活性几乎不受影响,活菌数在109 CFU/mL以上,说明该菌具备作为益生性饲料添加剂的基本条件.  相似文献