Enterococcus hirae in septicaemia of psittacine birds.     

L A Devriese  J I Cruz Colque  F Haesebrouck  M Desmidt  E Uyttebroek  R Ducatelle 《The Veterinary record》1992,130(25):558-559

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Comparative virulence of NAD-dependent and NAD-independent Actinobacillus pleuropneumoniae strains.     

P Dom  F Haesebrouck 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1992,39(4):303-306

The virulence of a NAD-independent Actinobacillus pleuropneumoniae serotype 2 strain and NAD-dependent serotype 2, 3 and 9 strains was compared under experimental conditions. Hysterectomy-derived piglets were inoculated endobronchially with 50-500 cfu of these strains. All 23 piglets inoculated with the NAD-dependent strains developed acute disease within 12 hours post inoculation. Twenty-two of these piglets died within 24 hours after the first clinical signs. Three of nine piglets inoculated with the NAD-independent strain did not develop clinical disease. In the other six piglets, disease signs were similar as in the piglets inoculated with the NAD-dependent strains. No differences in clinical disease were observed between colostrum deprived piglets and piglets that obtained colostrum from a SPF sow.  相似文献   

Effect of endobronchial challenge with Actinobacillus pleuropneumoniae serotype 9 of pigs vaccinated with a vaccine containing Apx toxins and transferrin-binding proteins     

Van Overbeke I  Chiers K  Ducatelle R  Haesebrouck F 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2001,48(1):15-20

The efficacy of a subunit vaccine containing the Apx toxins of Actinobacillus pleuropneumoniae and transferrin-binding proteins was determined. Ten pigs were vaccinated twice with the vaccine. Eight control animals were injected twice with a saline solution. Three weeks after the second vaccination, all pigs were endobronchially inoculated with 10(6.5) colony-forming units (CFU) of an A. pleuropneumoniae serotype 9 strain. In the vaccine group, none of the pigs died after inoculation. Only one pig of the control group survived challenge. Surviving pigs were killed at 7 days after challenge. The mean percentage of affected lung tissue was 64% in the control group and 17% in the vaccine group. Actinobacillus pleuropneumoniae was isolated from the lungs of all animals. The mean bacterial titres of the caudal lung lobes were 5.0 x 10(8) CFU/g in the control group and 3.0 x 10(6) CFU/g in the vaccine group. It was concluded that the vaccine induced partial protection against severe challenge.  相似文献   

Identification of aesculin-hydrolyzing streptococci, lactococci, aerococci and enterococci from subclinical intramammary infections in dairy cows     

Devriese LA  Hommez J  Laevens H  Pot B  Vandamme P  Haesebrouck F 《Veterinary microbiology》1999,70(1-2):87-94

Aesculin-hydrolyzing, catalase-negative, gram-positive cocci isolated from subclinical intramammary infections in dairy cows were identified to species level using growth characteristics and biochemical activity. The results indicated that the aesculin-hydrolyzing cocci associated with this type of infection are a very heterogenic group. S. uberis strains, including inulin- or beta-glucuronidase-negative isolates, accounted for only about one-third of the collection, and Enterococcus faecalis strains for one-fifth. Other species of some importance included (in descending order of isolation frequency) Aerococcus viridans, Streptococcus pluranimalium, Lactococcus garvieae, Streptococcus bovis and Streptococcus gallolyticus.  相似文献   

Effect of endobronchial challenge with Actinobacillus pleuropneumoniae serotype 10 of pigs vaccinated with bacterins consisting of A. pleuropneumoniae serotype 10 grown under NAD-rich and NAD-restricted conditions     

van Overbeke I  Chiers K  Donne E  Ducatelle R  Haesebrouck F 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2003,50(6):289-293

The efficacy of two bacterins containing an Actinobacillus pleuropneumoniae serotype 10 strain was evaluated. The bacterial cells constituting bacterin 1 and 2 were grown under nicotinamide adenine dinucleotide (NAD)-rich (low-adherence capacity to alveolar epithelial cell cultures) and NAD-restricted (high-adherence capacity to alveolar epithelial cell cultures) conditions, respectively. Ten pigs were vaccinated twice with the bacterin 1 and nine pigs with the bacterin 2. Ten control animals were injected twice with a saline solution. Three weeks after the second vaccination, all pigs were endobronchially inoculated with 106.5 colony-forming units (CFU) of an A. pleuropneumoniae serotype 10 strain. In the bacterin 1 and 2 group, three and two pigs died after inoculation, respectively. Only two pigs of the control group survived challenge. Surviving pigs were killed at 7 days after challenge. The percentage of pigs with severe lung lesions (> 10% of the lung affected) was 100% in the control group, 70% in the bacterin 1 group and 22% in the bacterin 2 group. Actinobacillus pleuropneumoniae was isolated from the lungs of all animals. The mean bacterial titres of the caudal lung lobes were 7.0 x 10(6) CFU/g in the control group, 6.3 x 10(5) CFU/g in the bacterin 1 group and 1.3 x 10(6) CFU/g in the bacterin 2 group. It was concluded that both bacterins induced partial protection against severe challenge. Furthermore, there are indications that the bacterin 2, containing A. pleuropneumoniae bacteria grown under conditions resulting in high in vitro adhesin, induced better protection than the bacterin 1.  相似文献   

Patterns of Mycoplasma hyopneumoniae infections in Belgian farrow-to-finish pig herds with diverging disease-course     

Vicca J  Maes D  Thermote L  Peeters J  Haesebrouck F  de Kruif A 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2002,49(7):349-353

Patterns of Mycoplasma hyopneumoniae (Mh) infections were investigated in five clinically infected herds and in five herds subclinically infected with Mh. In the clinically infected herds, housing and management conditions were good whereas these conditions were poor in the subclinically infected herds. In each herd, serum antibodies against Mh were detected in pigs of different ages and nasal swabs were taken for Mh detection using nested PCR (nPCR). The percentage of seropositive pigs in the clinically infected herds increased from 8% in pigs of 9 weeks to 52% in pigs of 18 weeks and seroconversion was most shown between 12 and 15 weeks. In the subclinically infected herds, the percentages increased from 2 to 24% and most of the pigs became seropositive between 15 and 18 weeks. The percentage of nPCR positive pigs at 6 weeks was 16 and 0% in the clinically and subclinically infected herds, respectively. The results demonstrate that the seroprevalences were higher in the clinically infected herds and that most of the pigs became infected with Mh at a younger age. It can be concluded that additional factors different from housing and management, like differences among Mh strains, may determine the infection pattern of Mh and the clinical course of the infection.  相似文献   

Occurrence of Salmonella in tortoises in a rescue centre in Italy     

Pasmans F  De Herdt P  Chasseur-Libotte ML  Ballasina DL  Haesebrouck F 《The Veterinary record》2000,146(9):256-258

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Dynamics of the development of Chlamydophila psittaci inclusions in epithelial and fibroblast host cells     

Escalante-Ochoa C  Ducatelle R  Haesebrouck F 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2000,47(5):343-349

The development of Chlamydophila psittaci (formerly Chlamydia psittaci, avian strains) inclusions in fibroblast L-929 and epithelial BGM cell lines was studied along the bacterial growth cycle using a BGM cell-adapted strain in the presence or absence of cycloheximide and cycloheximide + polyethylene glycol. Evolution of the inclusions was determined in terms of their number and size at 24, 30, 36, 48 and 54 h after infection. Significant differences in the chlamydial growth were found between both host cells, throughout the study. Higher numbers of inclusions (P < 0.05) were observed in L cells while larger inclusions (P < 0.01) were found in BGM cells. In both fibroblast and epithelial cells, inclusions showed a significant (P < 0.001) increase in size at the later times studied. Free extracellular chlamydial particles were noticed at 48 and 54 h post-infection (p.i.) in infected L cells, and at 54 h p.i. in BGM cells. Addition of cycloheximide or cycloheximide + polyethylene glycol had no significant effect on the number of inclusions or their size. The results suggest that host cell characteristics and innate compatibility between Chlamydophila strain and host cell are more important than host cell adaptation for the development of the microorganism.  相似文献   

Genetic diversity of livestock-associated MRSA isolates obtained from piglets from farrowing until slaughter age on four farrow-to-finish farms     

Marijke Verhegghe  Florence Crombé  Larissa J Pletinckx  Freddy Haesebrouck  Patrick Butaye  Lieve Herman  Marc Heyndrickx  Geertrui Rasschaert 《Veterinary research》2014,45(1)

During a previous longitudinal study, performed on four farrow-to-finish farms (A to D), samples were taken from twelve sows, their offspring, and the environment on various occasions over six months to study the MRSA presence. During the present study, a selection of the obtained MRSA isolates were typed by multiple-locus variable-number tandem-repeat analysis (MLVA), Pulsed Field Gel Electrophoresis (PFGE), spa typing, and SCCmec typing to study the genetic diversity of LA-MRSA isolates and to determine possible MRSA sources for pig(let)s. PFGE, spa typing, and SCCmec typing revealed the presence of one or few dominant genotype(s) per farm. In contrast, 212 MLVA types were detected on the four farms, forming one cluster on farm A, three on farm B, four on farm C and two on farm D. The genotype, found on farm A was unique for this farm. Farms B, C and D shared one cluster. In general, MLVA types from these clusters were isolated from piglets, sows, and the environment on various sampling events. Piglets carried MLVA types both related and unrelated to their mother sows’ MLVA types at farrowing and onwards. In conclusion, molecular typing revealed that within a farm one or a few dominant strain(s) are widespread. Potential MRSA sources for piglets were mother sows, the environment and other piglets.

Electronic supplementary material

The online version of this article (doi:10.1186/s13567-014-0089-4) contains supplementary material, which is available to authorized users.  相似文献   

The Influence of the Housing System on Salmonella Infections in Laying Hens: A Review     

S. Van Hoorebeke  F. Van Immerseel  F. Haesebrouck  R. Ducatelle  J. Dewulf 《Zoonoses and public health》2011,58(5):304-311

From 2012 onwards, housing of laying hens in conventional battery cages will be forbidden in the European Union and only enriched cages and non‐cage housing systems such as aviaries, floor‐raised, free‐range and organic systems will be allowed. Although this ban aims at improving the welfare of laying hens, it has also initiated the question whether there are any adverse consequences of this decision, especially with respect to the spread and/or persistence of zoonotic agents in a flock. A zoonotic agent that is traditionally associated with the consumption of eggs and egg products is Salmonella enteritidis. This paper provides a summary of the current knowledge regarding the direct and indirect effects of different housing systems on the occurrence and epidemiology of Salmonella in laying hen flocks.  相似文献