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1.
Hideki Takahashi Ayano Shimizu Tsutomu Arie Syofi Rosmalawati Sumire Fukushima Mari Kikuchi Yasufumi Hikichi Ayami Kanda Akiko Takahashi Akinori Kiba Kohei Ohnishi Yuki Ichinose Fumiko Taguchi Chihiro Yasuda Motoichiro Kodama Mayumi Egusa Chikara Masuta Hiroyuki Sawada Daisuke Shibata Koichi Hori Yuichiro Watanabe 《Journal of General Plant Pathology》2005,71(1):8-22
Lycopersicon esculentum cultivar Micro-Tom is a miniature tomato with many advantages for studies of the molecular biology and physiology of plants. To evaluate the suitability of Micro-Tom as a host plant for the study of pathogenesis, Micro-Tom plants were inoculated with 16 well-known fungal, bacterial, and viral pathogens of tomato. Athelia rolfsii, Botryotinia fuckeliana, Oidium sp., Phytophthora infestans, and Sclerotinia sclerotiorum caused typical symptoms and sporulated abundantly on Micro-Tom. Micro-Tom was resistant to Alternaria alternata, Corynespora cassiicola, and Fusarium oxysporum. When Micro-Tom was inoculated with 17 isolates of Ralstonia solanacearum, many isolates induced wilt symptoms. Agrobacterium tumefaciens also was pathogenic, causing crown galls on stem tissue after needle prick inoculation. In Micro-Tom sprayed with Pseudomonas syringae pv. tomato, P. s. pv. tabaci, or P. s. pv. glycinea, bacterial populations did not increase, and yellow lesions appeared only on leaves sprayed with P. s. pv. tomato. Tomato mosaic virus, Tomato aspermy virus, and Cucumber mosaic virus systemically infected Micro-Tom, which developed symptoms characteristic of other cultivars of tomato after infection with the respective virus. These results indicated that Micro-Tom was generally susceptible to most of the important tomato pathogens and developed typical symptoms, whereas certain pathogens were restricted by either hypersensitive resistance or nonhost resistance on Micro-Tom. Therefore, an assortment of Micro-Tom–pathogen systems should provide excellent models for studying the mechanism of susceptible and resistant interactions between plants and pathogens. 相似文献
2.
Ayano OMURA Hideki ENDO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(1):43-47
The digestive organs in decapodiform cephalopod species morphologically vary by
individual lifestyle. We examined the following six species of adult decapodiformes
cephalopods representing different habitats: Todarodes pacificus, Loligo
bleekeri, Loligo edulis, Watasenia
scintillans (pelagic), Sepia lycidas and Euprymna
morsei (benthic). L. bleekeri and L. edulis
possess a bursiform cecal sac connected to the cecum. Pelagic species have a single
digestive gland smaller than in benthic species. T. pacificus has an oval
digestive gland larger than that of L. bleekeri and L.
edulis, which possess withered-looking and smaller digestive glands. In
contrast, the digestive glands in benthic species are paired. S. lycidas
and E. morsei have well-developed and larger digestive glands than those
of the pelagic species. Well-developed digestive duct appendages are found in benthic
species. In qualification of the mass of digestive organs, pelagic species have smaller
stomachs, digestive glands and digestive ducts’ appendages than benthic species. Because
pelagic species need to swim, they may possess smaller stomachs and larger cecums for more
rapid digestion. A smaller digestive gland may have the advantage of reducing the body
weight in pelagic species for rapid swimming. In contrast, since benthic species require a
longer time for digestion than pelagic species, they compact more food in their stomachs
and absorb nutrients via more organs, such as the digestive grand and digestive duct
appendages, in addition to cecum. 相似文献
3.
The transition time from the juvenile phase to the adult phase differs in soybean cultivars ‘Enrei’ and ‘Peking’ 下载免费PDF全文
Suguru Ozawa Katsunori Hatakeyama Yoshihito Takahata Shuji Yokoi 《Plant Breeding》2017,136(3):386-392
Plants develop juvenile phase to adult phase in vegetative stage. Although soybean is a very important crop worldwide, there has been only one study of the juvenile–adult phase change. In this study, we determined that the juvenile–adult phase change occurred at different stages in two soybean cultivars that differ in their photosensitivity. Cultivar ‘Enrei’ (E1e2e3E4) is weakly photosensitive and cultivar ‘Peking’ (E1E2E3E4) is strongly photosensitive. In ‘Enrei’, the leaf size gradually increased at a constant leaf position regardless of the difference in day length. In ‘Peking’ plants transferred to short‐day conditions at several leaf development stages, leaf size gradually increased at different leaf positions. Expression of miR156 by ‘Enrei’ transferred to short‐day conditions had nearly the same pattern as that of ‘Enrei’ grown under long‐day conditions. In ‘Peking’, the expression of miR156 had different patterns in younger leaves of plants subjected to either a short‐day treatment or long‐day conditions. These results indicate that the E2 and E3 loci that regulate photosensitivity also regulate the expression of miR156 and the juvenile–adult phase change in soybean. 相似文献
4.
Establishment of a potency test by ELISA for a rabies vaccine for animal use in Japan 总被引:2,自引:0,他引:2
Gamoh K Shimazaki Y Senda M Makie H Itoh O Muramatsu M Hirayama N Hatakeyama H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(6):685-688
The ELISA we developed was able to determine the antigen content and was suitable for a potency test, and we described a relative potency assay method which determines the potency of test vaccines by comparing the ELISA value of a test vaccine to that of a reference vaccine. In the present study, we standardized the reference vaccine used for determining the potencies of test vaccines, and established a potency test by ELISA. We evaluated the proposed reference vaccine by the neutralizing antibody responses in dogs after vaccination, by the challenge protection test in guinea pigs (GP potency test), which is the earlier official potency test used in Japan, and by the NIH potency test, which is widely used throughout the world. The results showed that a 4-fold dilution of the proposed reference vaccine induced sufficient immunity in dogs. A 3-fold dilution of the proposed reference vaccine passed the GP potency test. The international units (IU) calibrated by the NIH potency test were 3.7 IU/dose. From the results and the WHO recommendation that veterinary rabies vaccines should have a potency of at least 1.0 IU/dose, we determined to dilute the proposed reference vaccine by 3 fold and regarded it as the reference vaccine. Finally, we confirmed that there is a good agreement between the results of the potency test by ELISA and the results of the GP potency test. The establishment of the potency test by ELISA has made it possible to monitor the potency in the production process and has contributed to the stable production of the vaccine. 相似文献
5.
In this study, the genetic relatedness of 22 low chill requiring walnut genotypes adapted to the south east Mediterranean region of Turkey was analysed by amplified fragment length polymorphism (AFLP) markers. Relatively low level of genetic variation was detected among the genotypes examined by five AFLP primer combinations, suggesting that these walnut genotypes selected predominantly for their low chill requirement have relatively narrow genetic base. In addition, the geographical proximity of the genotypes analysed was not correlated with their level of genetic relatedness. These results have implications for walnut breeding and conservation. 相似文献
6.
A new monocyclic triterpene ester (1) was isolated from the rhizomes of Iris germanica from Turkey. Structure elucidation of compound (1) was carried out by the combined application of HMQC, HMBC, DEPT, COSY and NOESY experiments and named as iristectorone K. 相似文献
7.
Kazuyuki SUZUKI Toshio SHIMAMORI Ayano SATO Kenji TSUKANO Masakazu TSUCHIYA Jeffrey LAKRITZ 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(8):977-979
The aim of the present study was to compare the ability of the commercially available portable test system (PTSTM) to detect endotoxin activity in bovine serum, with that of the traditional LAL-kinetic turbidimetric (KT) and chromogenic (KC) assays. Prior to testing, serum samples, which were obtained from endotoxin-challenged cattle, were diluted 1:20 in endotoxin-free water and heated to 80°C for 10 min. The performance of the PTSTM was not significantly different from that of the traditional LAL-based assays. The results using PTSTM correlated with those using KT (r2=0.963, P<0.001) or KC assays (r2=0.982, P<0.001). Based on these findings, the PTSTM could be applied as a simplified system to assess endotoxin activity in bovine serum. 相似文献
8.
Ayano OMURA Wataru ANZAI Daisuke KOYABU Hideki ENDO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(9):1043-1048
Clarification of the trunk structure in Urodela is important in understanding the
locomotive evolution of basal tetrapods. The components of the muscular trunk wall among
Urodela using different modes of locomotion were compared. Since the whole trunk may be
used for swimming and the effect of limbs may be small in the more aquatic species, they
showed smaller differences in the trunk muscles among anterior, middle and posterior
sections of the trunk. By contrast, in the more terrestrial species, the dorsal and
abdominal muscles are larger in the middle section than those in the anterior and
posterior sections. High compressive stresses occur in the supporting limbs and their
insertion at the trunk on the ventral side, and spread from the forelimbs along the back
to the supporting hindlimbs on the dorsal side. Tensile stresses occur in the middle
ventral part. The components of the trunk muscles among the three sections may reflect
differences in stresses occurring in the trunk of the more terrestrial species. The
findings also suggest that in the middle section, larger dorsal muscles for stiffening the
back to maintain posture and larger abdominal muscles are responsible for balancing the
body weight while it is supported by the limbs in the more terrestrial species. 相似文献
9.
M. Kitase S. Hatakeyama T. Mizoguchi Y. Maeda 《Water, air, and soil pollution》2001,130(1-4):1463-1468
Copper plates were exposed under shelters at 13 sampling sites in East Asia and their corrosion was analyzed. The corrosion products were first dissolved in water and then oxalic acid. Sulfate, nitrate and chloride in the solutions were measured by ion chromatography. The amounts of the three anions significantly differed depending on the atmospheric environment at the sites. Sulfate was a major part of the anions at Chongqing and Shanghai in China. Especially, at the urban sites in Japan, nitrate remarkably changed with the seasons, and often became the large anionic component in the summer. The amounts of chloride at most sites were at higher concentration levels compared with those at the rural sites in Japan. The anions in the copper corrosion must mainly reflect the impact of acid deposition. 相似文献
10.
Jin DH Zhang Y Suzuki Y Naganuma T Ogawa T Hatakeyama E Muramoto K 《Journal of agricultural and food chemistry》2000,48(11):5450-5454
Protein hydrolysates, prepared by enzymatic digestion of soybean protein and egg white albumin using several proteases, inhibited the crystal growth of calcium carbonate. Each hydrolysate showed different inhibitory activities, suggesting the key role of peptide structures in the inhibition. The deamidation of protein hydrolysates by glutaminase increased not only the inhibitory activity toward the crystal growth of calcium carbonate but also the resistance of the hydrolysates against peptic digestion. Furthermore, the addition of sodium chloride, citric acid, or lactose into the reaction mixture enhanced the inhibitory activity. The protein hydrolysates inhibited both nucleation and crystal growth of calcium carbonate and also affected the crystal morphology. 相似文献