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排序方式: 共有60条查询结果,搜索用时 171 毫秒
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Yazawa M Okuda M Setoguchi A Iwabuchi S Nishimura R Sasaki N Masuda K Ohno K Tsujimoto H 《American journal of veterinary research》2001,62(10):1539-1543
OBJECTIVE: To measure telomere length and telomerase activity in naturally occurring canine mammary gland tumors. SAMPLE POPULATION: 27 mammary gland tumor specimens obtained during resection or necropsy and 12 mammary gland tissue specimens obtained from healthy (control) dogs. PROCEDURE: Telomere length in tissue specimens was measured by use of restriction endonuclease digestion and Southern blot analysis. Telomerase activity was measured by use of a telomeric repeat amplification protocol assay. RESULTS: Telomere length in mammary gland tumors ranged from 11.0 to 21.6 kilobase pairs (kbp; mean +/- SEM, 14.5+/-0.5 kbp) but did not differ among tumor types. Telomeres in mammary gland tumors were slightly shorter than in normal tissue specimens, but telomere length could not be directly compared between groups, because mean age of dogs was significantly different between groups. Age was negatively correlated with telomere length in control dogs but was not significantly correlated with length in affected dogs. Telomerase activity was detected in 26 of 27 mammary gland tumors and in 4 of 12 normal tissue specimens. However, telomerase activity and telomere length were not correlated in tumor specimens. CONCLUSIONS AND CLINICAL RELEVANCE: Telomere length is maintained in canine mammary gland tumors regardless of the age of the affected dog. Measurement of telomere length may be a useful tool for monitoring the in vivo effects of telomerase inhibitors in dogs with tumors. 相似文献
3.
Iwabuchi S Suzuki K Sakemi Y Imayoshi K Kuwahara E Asano R 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(9):1033-1036
The effects of intravenous infusion of hypotonic lactated Ringer's (LR: n=14) on plasma volume and venous blood gases were compared to those of hypotonic Ringer's solutions (RS: n=7) in diarrheic Japanese Black breed calves with metabolic acidosis. Venous blood samples were collected immediately before and after, and at 24 hr after the fluid infusion therapy. The LR and RS infusions increased relative plasma volume to 147.1 +/- 25.5% and 134.2 +/- 18.6%, respectively, just after the fluid therapy. The LR infusion induced an increase in the BE value (+5.1 +/- 4.8 mM) at 24 hr compared to that of RS. LR infusion should be explored as a treatment for dehydration and moderate metabolic acidemia caused by naturally occurring diarrhea in calves. 相似文献
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Suzuki K Abe I Iwabuchi S Tsumagari S Matsumoto T Asano R 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(8):699-703
After intravenous (i.v.) infusion of various volumes of 1.35%-isotonic sodium bicarbonate solution (ISB), acid-base equilibrium, blood pressure, plasma volume and biochemical parameters in healthy Holstein calves were studied. Four calves each were randomly assigned to the low-dose (LD; i.v. infusion of 5 ml/kg ISB), middle-dose (MD; i.v. infusion of 10 ml/kg ISB) and the high-dose groups (HD; i.v. infusion of 15 ml/kg ISB). Administration volumes of ISB in the LD, MD and HD groups were decided based on the first half volumes of 5, 10 and 15 mEq of base requirement by the acceptable equation. Systemic, pulmonary artery and central venous pressures, cardiac output and plasma osmotic pressure were not changed by ISB infusion and remained constant throughout the experiment for all groups. There was good correlation (r(2) = 0.950) between relative changes in base excess and infused volume of bicarbonate (y=2.491x). The coefficient of distribution for bicarbonate ions was calculated to be 0.401 (=1/2.491). Therefore, it is suggested that a value of 0.4 would be most appropriate when calculating the base requirements in calves. Therefore, the first half volumes of ISB correcting base deficits of 5, 10 and 15 mEq in calves were estimated to be 6, 12 and 18 ml/kg, respectively. On the basis of the findings in this study, ISB may be used to correct metabolic acidosis without altering the plasma osmotic pressure, hemodynamic status and respiratory function in the calves. 相似文献
6.
Suzuki K Kato T Tsunoda G Iwabuchi S Asano K Asano R 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(12):1173-1175
The effect of 1.35% isotonic sodium bicarbonate solution (ISB) administered intravenously on acid-base equilibrium was examined in 18 acidemic Japanese black beef calves with spontaneous diarrhea. The infusion volumes of ISB were decided based on the first half volumes of base needed. In 72.2% (13/18) of calves, improvement of acidemia was detected. There was good correlation (r=0.693, p<0.01) between infused volume of ISB and changes in base excess (y=1.097x + 4.762). Infusion volumes of ISB were 7.5, 10.2, 12.9 and 15.7 ml/kg, respectively, enough to correcting the first half of 5, 10, 15 and 20 mEq/l of base deficit in acidemic calves. Our finding suggested that ISB could be used to correct metabolic acidosis without altering electrolyte concentrations in calves. 相似文献
7.
Optimal application timing of simeconazole granules for control of rice kernel smut and false smut 总被引:1,自引:0,他引:1
Mikio Tsuda Masashi Sasahara Toshiaki Ohara Shigehiro Kato 《Journal of General Plant Pathology》2006,72(5):301-304
We investigated the optimal timing of simeconazole (RS-2-(4-fluorophenyl)-1-(1H-1,2,4-triazol-1-yl)-3-trimethylsilylpropan-2-ol) application for controlling rice kernel smut in field trials in Miyagi Prefecture,
Japan, using formulations of simeconazole (1.5% granules). The field tests revealed that a submerged application of simeconazole
granules (450–600 g ai/ha) at 1–5 weeks before heading was highly effective against kernel smut, with treatments 1–2 weeks
before heading being the most effective. Submerged application of the fungicide at 2–5 weeks before heading was also highly
effective against false smut, with treatment 3 weeks before heading being the most effective. These periods overlap the timing
for optimal application of simeconazole to control rice sheath blight and ear blight. Consequently, we concluded that treatment
with simeconazole 2–3 weeks before heading can be a useful tool for controlling all four diseases. 相似文献
8.
Nobuaki Tamai Chisato Takenaka Shigehiro Ishizuka Takafumi Tezuka 《Journal of Forest Research》2001,6(4):311-313
The relationship between soil air volume and CH4 consumption in brown forest soils was investigated. The CH4 consumption was negatively affected when the soil air volume decreased in experiments carried out bothin situ and in the laboratory. A strong dependence of the CH4 consumption on the soil air volume was found in the laboratory experiment. These results indicate the possibility that the
soil air volume is the primary rate-limiting factor for the CH4 consumption in forest soil. Thus our work suggests that the other factors by which CH4 consumption might be affected should be examined under the same air volume condition in the soil. To obtain accurate data,
we need to be careful not to change the air volume by trampling or disturbing during the measurement of the natural methane
fluxin situ. 相似文献
9.
Hashizume M Chiba M Shinohara A Iwabuchi S Sasaki S Shimoda T Kunii O Caypil W Dauletbaev D Alnazarova A 《Public health nutrition》2005,8(6):564-571
OBJECTIVES: To investigate the prevalence of anaemia and iron deficiency and vitamin A status among school-aged children in rural Kazakhstan and identify factors associated with anaemia in this population. DESIGN: A cross-sectional design. SETTING: School-aged children in rural Kazakhstan. SUBJECTS: Socio-economic and anthropometric information was collected from 159 school-aged children living in the Kzyl-Orda region of Kazakhstan. Blood samples were collected and the concentrations of haemoglobin (Hb), serum iron, serum ferritin (SF), erythrocyte protoporphyrin (EP), serum retinol and beta-carotene, total iron binding capacity (TIBC), transferrin saturation (TS) and other haematological indices were measured. RESULTS: Among the 159 children, the prevalence of anaemia and iron deficiency defined by the multiple criteria model (SF, TS and EP) was 27% and 13%, respectively. Nine per cent had iron-deficiency anaemia and 21% had serum retinol value < 1.05 micromol l(-1). Mean SF and serum iron concentrations and TS were significantly lower in anaemic children than in their non-anaemic peers, while TIBC and EP were significantly higher in children with anaemia. Hb was significantly correlated with serum iron and retinol concentrations. Serum retinol and SF concentrations and mean corpuscular volume were significantly correlated with Hb by multiple regression analysis. CONCLUSIONS: Anaemia among school-aged children in rural Kazakhstan appears to be related to iron indices and vitamin A status. 相似文献
10.
A protein that binds to a cis-acting element of wheat histone genes has a leucine zipper motif 总被引:34,自引:0,他引:34
T Tabata H Takase S Takayama K Mikami A Nakatsuka T Kawata T Nakayama M Iwabuchi 《Science (New York, N.Y.)》1989,245(4921):965-967