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Junki Ito Shigeki Hasegawa Kounosuke Fujita Shizuhiko Ogasawara Tamio Fujiwara 《Soil Science and Plant Nutrition》2013,59(2):385-393
Six year-old Japanese pear (Pyrus seratina Reheder cv. Kosui) trees grafted on P. serotina cv. Nihonyamanashi were grown in containers filled with Granite Regosol under glasshouse conditions. At different stages of fruit growth, pear trees were exposed to an elevated CO2 concentration (130 Pa CO2 ) along with a control (35 Pa CO2). For one group of plants, CO2 enrichment was applied for 79 d from 52 d after full bloom (DAB) to fruit maturity (long-term CO2 enrichment) and for another group the same treatment was applied for 35 d from 96 DAB to fruit maturity (short-term CO2 enrichment). The effects of the elevated CO2 concentration on vegetative growth, mineral contents, and fruit production and quality were examined. Long-term CO2 enrichment enhanced vegetative growth, without any significant effect on the mineral contents in either flower bud or fruit except for a remarkable increase in the K content. Long-term CO2 enrichment increased the fruit size and fresh weight, but had no significant effect on the fruit quality. On the other hand, the short-term CO2 enrichment did not induce any significant change in the fruit size but increased the fruit sugar concentration. Along with the reduction of the sorbitol concentration in fruit, the fructose and sucrose concentrations increased and these changes occurred earlier at elevated CO2 than at ambient CO2 concentrations. From these results, we concluded that the effect of CO2 enrichment on fruit growth varies depending upon the growth stages of fruit: during the initial and fruitlet stages when fruit expansion occurs, CO2 enrichment increases the fruit size, whereas, during maturation when fruit expansion has slowed down and sugar accumulation in fruit is active, it increases the fruit sugar concentration. 相似文献
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Yuri ABE Tomokazu TAMURA Shiho TORII Shiho WAKAMORI Makoto NAGAI Kazuya MITSUHASHI Junki MINE Yuri FUJIMOTO Naofumi NAGASHIMA Fumi YOSHINO Yukihiko SUGITA Takushi NOMURA Masatoshi OKAMATSU Hiroshi KIDA Yoshihiro SAKODA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(1):61-70
In our previous study, we genetically analyzed bovine viral diarrhea viruses (BVDVs)
isolated from 2000 to 2006 in Japan and reported that subgenotype 1b viruses were
predominant. In the present study, 766 BVDVs isolated from 2006 to 2014 in Hokkaido,
Japan, were genetically analyzed to understand recent epidemics. Phylogenetic analysis
based on nucleotide sequences of the 5′-untranslated region of viral genome revealed that
766 isolates were classified as genotype 1 (BVDV-1; 544 isolates) and genotype 2 (BVDV-2;
222). BVDV-1 isolates were further divided into BVDV-1a (93), 1b (371) and 1c (80)
subgenotypes, and all BVDV-2 isolates were grouped into BVDV-2a subgenotype (222). Further
comparative analysis was performed with BVDV-1a, 1b and 2a viruses isolated from 2001 to
2014. Phylogenetic analysis based on nucleotide sequences of the viral glycoprotein E2
gene, a major target of neutralizing antibodies, revealed that BVDV-1a, 1b and 2a isolates
were further classified into several clusters. Cross-neutralization tests showed that
BVDV-1b isolates were antigenically different from BVDV-1a isolates, and almost BVDV-1a,
1b and 2a isolates were antigenically similar among each subgenotype and each E2 cluster.
Taken together, BVDV-1b viruses are still predominant, and BVDV-2a viruses have increased
recently in Hokkaido, Japan. Field isolates of BVDV-1a, 1b and 2a show genetic diversity
on the E2 gene with antigenic conservation among each subgenotype during the last 14
years. 相似文献
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Shinji Kajihara Junki Itou Noritoshi Katsutani Tanajuro Goto Hideo Shimaji 《Scientia Horticulturae》2009
The arching and high-rack culture systems were developed and patented by Japanese rose growers. Both culture systems have bent canopies (lower bent shoots). In the arching culture system, shoots sprouting from the crown are harvested as cut flowers. However, the high-rack culture system also has a bent canopy originating from the mother stem (upper bent shoots) and flower stems sprout and is harvested at the top of each mother stem. Partitioning of photosynthates originating from bent shoots in arching and high-rack culture systems of rose production was investigated to elucidate how carbohydrates are re-allocated from the bent shoots in different culture systems of roses. At the flowering stage in both culture systems, 50–70% of 13C-photosynthates originated from bent shoots were exported to other parts within 72 h after 13CO2 feeding to the bent shoot. In the arching culture system, photosynthates from lower bent shoots were partitioned mainly to the roots and crown. Similarly, in the high-rack culture system, between 71 and 86% of the exported carbon from the bent shoots were allocated to below the point of bending (roots + crown + mother stems) and only 9–28% was allocated to flowering shoots above the point of bending. In both culture systems, photosynthate translocation from the lower bent shoot directly to flowers was low. Accordingly, bent shoots in rose plants acted as a source of photosynthates, independent of culture system. The height of the bent shoots determined for a great deal in the re-allocation of the photosynthates, and provides a partial explanation for difference in production of cut roses. 相似文献
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Pathogenicity of border disease virus FNK2012-1 strain isolated from a pig in
the natural host,sheep
Tomokazu TAMURA Junki MINE Shiho TORII Yuri FUJIMOTO Masatoshi OKAMATSU Yoshihiro SAKODA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(3):341-343
A first isolation of border disease virus (BDV) in Japan was from a pig on a farm without
keeping any ruminants. Our previous study showed that this BDV, termed the FNK2012-1
strain, replicated inefficiently in swine-derived cells compared with those of ruminant
origin. Pigs inoculated with this virus showed neither clinical symptoms nor viremia. In
this study, we evaluated the pathogenicity of the FNK2012-1 strain in sheep, its natural
host. The inoculated sheep showed clinical symptoms and transient viremia. Seroconversion
was observed in the inoculated sheep. These results suggest that the FNK2012-1 strain was
introduced from sheep and has not yet adapted to swine. Therefore, surveillance of border
disease in Japan is necessary among both the swine and ruminant populations. 相似文献
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Akiyoshi EGASHIRA Nobuhiko YAMAUCHI Keiko TANAKA Chihiro MINE Hitomi OTSUBO Masao MURAKAMI Md. Rashedul ISLAM Misako OHTSUKA Naomi YOSHIOKA Takashi KURAMOTO 《The Journal of reproduction and development》2015,61(6):595-600
The presence of multinucleated blastomeres (MNBs) in embryos is associated with poor developmental
competence in assisted reproductive technologies. This phenomenon is observed not only in humans but also in
other animal species. The purpose of the present study was to investigate the characteristics of embryos with
MNBs (MNB embryos) that could be utilized in embryo transfer. The developmental rate of MNB embryos to the
blastocyst stage (50.8%) was significantly lower than that of normal embryos (73.3%) (P < 0.05). The
clinical pregnancy rates of fresh embryo transfer (ET) using day 2 or day 3 embryos were significantly lower
in MNB embryos (5.1%) compared with normal embryos (24.0%) (P < 0.05). In the case of frozen-thawed ET
using a single vitrified/warmed blastocyst, however, the clinical pregnancy rate of MNB embryos was close to
that of normal embryos (59.1% vs. 52.8%). Thus, the findings of the present study suggest
that the frozen-thawed ET of MNB embryos might improve the potential for implantation followed by successful
pregnancy. 相似文献
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Fitrine EKAWASTI Kazuya KITAGAWA Hiroshi DOMAE April Hari WARDHANA Junki NAGASAWA Tomoyuki SHIBAHARA Masaharu TOKORO Kazumi SASAI Makoto MATSUBAYASHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(8):1240
Oocysts of Isospora sp. were detected in the feces of a veiled chameleon (family Chamaeleonidae; Chamaeleo calyptratus) kept at a zoo in Ishikawa, Japan. Phylogenetic analysis placed the sequence in the cluster of Isospora spp. isolated from reptiles. Based on a comparison of morphological data of ten previously reported Isospora species from the Chamaeleonidae family, this isolate was morphologically similar to I. jaracimrmani, which has been considered to be a virulent species. This case study suggests the possibility that species of Isospora might not always cause disease because the animal that shed these oocysts showed no symptoms for more than two months. 相似文献
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Reda Elwany Abedelhaleem Moghaieb Hirofumi Saneoka Junki Ito Kounosuke Fujita 《Soil Science and Plant Nutrition》2013,59(2):377-385
Three commercial tomato cultivars (UC-97, Momotaro, and Edkawi) were subjected to a gradual increase of NaCI concentrations and the effect on biomass production and its parameters was compared. The data indicated that salinity reduced plant growth and the reduction was more pronounced in UC-97 and less in Edkawi. The apparent photosynthetic rate (P o) was also depressed by the salt treatment and the depression was more remarkable in UC-97 and less in Edkawi. Edkawi shoot showed a much higher concentration of sodium ion and proline compared with the other cultivars, which may result in the maintenance of a higher turgor potential. In all the cultivars examined the stem diameter decreased after the beginning of exposure to light and recovered after the light was turned off. The decrease in the stem diameter during day light was enhanced and the recovery at night decreased after 1 d of salt treatment and the changes in the stem diameter were less conspicuous in Edkawi than in the other cultivars. These results suggest that Edkawi is more tolerant to salinity than the other cultivars due to a higher ability of maintaining the root function for the uptake and supply of water to shoot under salinity conditions but not due to the adjustment of transpiration from stomata. 相似文献
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