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A modified electrometric cholinesterase method has been described for use in ruminants exposed to organophosphate insecticides. The method was used to measure cholinesterase activities in the plasma and erythrocytes of sheep, goats and cattle treated with organophosphate insecticides under field conditions. The animals were treated topically to control ectoparasites with malathion (0.05%) or diazinon (0.06%). The treated animals did not suffer from adverse effects related to cholinesterase inhibition. However, 2 h after treatments, plasma and erythrocyte cholinesterase activities significantly decreased in sheep by 20% and 39% and in goats by 18% and 30%, respectively, when compared with respective untreated control values. Diazinon did not significantly affect plasma and erythrocyte cholinesterase activities in the cattle. The results suggest that the described electrometric method could be efficiently used for detecting cholinesterase inhibition in ruminants exposed to organophosphate insecticides.  相似文献   
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Effects of exposing quail eggs to high temperature on the heat tolerance ability and productivity of birds were investigated. Four groups of 600 fertile eggs were randomly selected; the first group was incubated under 37.5 °C and the hatched chicks were reared under a gradual decrease in temperature from 35 to 24 °C (Control). The second group was exposed to 39.1 °C for 2 h/day during 4–14 days of embryogenesis and the hatched chicks were reared under a gradual decrease in temperature from 35 to 24 °C. The third group was incubated under 37.5 °C and the hatched chicks were exposed to 39?±?1 °C for 2 h/day during 4–14 days of age. The fourth group was exposed to 39?±?1 °C for 2 h during 4–14 days of embryogenesis and the hatched chicks were exposed to 39?±?1 °C for 2 h/day during 4–14 days of age. The temperature applied changed (P?<?0.01) embryo weight and incubation period. Birds exposed to high temperature during brooding had superior growth performance, dressed carcass, body temperature and health traits. Birds subjected to 39?±?1 °C during brooding exhibited decreased feed consumption and body weight gain. Finally, this work suggests that thermal acclimation during embryogenesis might offer a practical method for easing heat stress.

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A comparative study was conducted to evaluate four previously reported methods that proved to have a recovery greater than 80% for the determination of different levels of ochratoxin A (OTA) in green and roasted coffee beans and to select an accurate, sensitive, and less-expensive technique between the existing methods. The results indicated that the Association of Official Analytical Chemists (AOAC) official method for the extraction of OTA in green coffee and determination by high-performance liquid chromatography (HPLC) is recommended as an efficient method for the routine analyses of OTA in green and ground roasted coffee beans. This method proved to be an accurate, sensitive, and less-expensive method that employs routine materials and available equipment. Although the immunoaffinity column/HPLC procedure tested showed a significantly higher percentage than the AOAC recommended method, it is recommended for use in processed coffee beans where low concentrations of OTA may be expected to be detected.  相似文献   
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