首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   12篇
  免费   0篇
  3篇
综合类   2篇
农作物   2篇
畜牧兽医   4篇
园艺   1篇
  2021年   1篇
  2018年   1篇
  2015年   1篇
  2014年   1篇
  2012年   2篇
  2011年   3篇
  2010年   1篇
  2008年   1篇
  1999年   1篇
排序方式: 共有12条查询结果,搜索用时 31 毫秒
1.
With the aim of extending the vase life of cut rose flowers, we investigated whether the application of salicylic acid (SA), pre- and postharvest, could improve this. For this purpose, two individual trials were conducted, using a completely randomised design, with three replications. In the first trial, aqueous solutions of 0, 0.5, 1.0 and 1.5 mM SA were sprayed on roses grown in a controlled greenhouse once the flower buds had appeared, and the second stage was performed two weeks before harvest. The preharvest SA application at 1.5 mM concentration prolonged the vase life of cut roses by improving membrane stability, and decreasing the lipid peroxidation properties. In the second trial, continuous exposure to (0, 0.5, 1.0 and 1.5 mM), and pulses of (0, 5, 10 and 15 mM), SA significantly extended the vase life. Flowers treated with 1.5 (continuous exposure) and 15 mM (pulse) SA showed a significant decrease in weight loss, and antioxidant activity increased, compared to the control solution, but the flowers in the control solution indicated an increase in weight loss. The results show that pre- and postharvest SA application prolonged the vase life of cut roses through improving CAT and POD activity, and decreasing lipid peroxidation.  相似文献   
2.
3.
Changes in the properties of solonetzic soil associations (chestnut solonetzic soils and chestnut solonetzes) in the dry steppe after their reclamation have been studied for 30 years. The reclamation included the deep three-tier plowing and the approach of rotary tillage. A single rotary tillage operation resulted in the formation of fine aggregates of equal sizes in the plow layer; any morphological features of the restoration of solonetzic pedogenesis are absent. The atmospheric moisture easily penetrates into the soil, and soluble salts are leached off to a great depth. In 30 years since the soil amelioration with the use of a PMS-70 rotary tiller, the humus content has increased up to 3.3% in the upper 20-cm-thick layer and up to 2.4% in the layer of 20–40 cm. The content of adsorbed Na+ in the 20- to 30-cm-thick layer has decreased to 10.6% of the cation exchange capacity (in comparison with 19.8% in the nonreclaimed soil). The spatial heterogeneity of the soil cover has decreased in comparison with that prior to the reclamation. During the entire observation period, crop yields gained from the fields reclaimed with the use of the rotary tiller have been by 25–60% higher in comparison with those on the fields with traditional treatments.  相似文献   
4.
Objective To evaluate the efficacy of a chemically modified and cross‐linked derivative of hyaluronan (CMHA‐SX) for treatment of corneal epithelial abrasion and standardized alkali burn injuries. Animals Twelve female New Zealand white rabbits in two groups were used. Procedures Bilateral 6‐mm diameter corneal epithelial abrasions were made in each of six rabbits in one group and 6‐mm standardized alkali burn injuries were made in the second group. A 1% CMHA‐SX formulation was applied topically four times per day in right eye of each rabbit for 1 week, and phosphate buffered saline (PBS) was placed in left (control) eye of each rabbit. The wound size was determined by staining with 1% fluorescein and photographed at the slit lamp with a digital camera at 0, 1, 2, 3 days postoperatively in the first group and 0, 1, 2, 3, 7, 12 days in the second group. Rabbit corneas were collected for histological examination on day 7 in the first group and day 12 in the second group. Results Closure of corneal wound in the abrasion model was complete in the CMHA‐SX treated eye by 48 h. The wound closure rate and thickness of the central corneal epithelium in the CMHA‐SX treated group was greater than in control eyes for both the abrasion and alkali burn injuries. Moreover, the CMHA‐SX treated cornea exhibited better epithelial and stromal organization than the untreated control cornea. Conclusions Chemically modified and cross‐linked derivative of hyaluronan improved corneal wound healing and could be useful for treating noninfectious corneal injuries.  相似文献   
5.
Column experiments were conducted over 45 d to determine the degree of P mobility. The sandy loam soil was spiked with 200 mg P kg–1 and 5% organic residues. The treatments included: control without any water‐soluble P and plant residues, potato, wheat, water‐soluble P fertilizer, wheat + water‐soluble P, and potato + water‐soluble P. Each column was leached with distilled water, and leachates were collected and analyzed for P, K+, Ca2+, Mg2+, along with pH and EC. Sequential extraction was performed on soil samples at the end of leaching column experiments. The relatively high initial concentration of P in the leachates decreased to more stable values after 15 d which can be attributed to the colloid‐bound P. The P concentrations in the leachates fluctuated between 8 and 220 mg L–1 in the water‐soluble–P fertilizer treatment, between 0.80 and 230 mg L–1 in the potato + water‐soluble‐P treatment, and between 0.90 and 214 mg L–1 in the wheat + water‐soluble P treatment. Leaching loss of P mainly occurred in the 15 d of leaching, accounting for 94%, 88%, and 65% of total P leached in wheat + water‐soluble‐P, potato + water‐soluble‐P, and water‐soluble‐P treatments, respectively. Maximum amount of P leached was found from an exponential kind model and was in the range 0.45 mg kg–1 to 125.4 mg kg–1 in control and potato + water‐soluble‐P treatments, respectively. Sequential extraction results showed that in control and amended soils the major proportion of P was associated with Ca. The leachate samples in all treatments were saturated with respect to hydroxyapatite, β‐tricalcium phosphate, and octacalcium phosphate up to 20 d of leaching, whereas they were undersaturated with respect to Mg‐P minerals through the entire leaching experiment.  相似文献   
6.
7.
ObjectiveTo evaluate the clinical and physiological effects of epidural injection of ketamine in camels.Study designRandomized prospective study.AnimalsTen healthy immature male dromedary camels.MethodsKetamine was administered epidurally at doses of 1 and 2 mg kg?1 (five animals in each treatment). The drug was injected into the first intercoccygeal epidural space. Anti-nociception, sedation, ataxia, and effect on cardiopulmonary, rectal temperature and some selected haematological parameters were recorded at different intervals before (baseline) and after the drug administration. Data were analyzed by anova or U Mann–Whitney tests, as relevant and significance was taken as p < 0.05.ResultsEpidural ketamine at the 2 mg kg?1 dose produced complete anti-nociception in the tail, anus and perineum, whilst the 1 mg kg?1 dose produced complete anti-nociception only in the tail. Epidural ketamine resulted in mild to moderate sedation at the 1 mg kg?1 dose and deep sedation at the 2 mg kg?1 dose. Ataxia was observed in all test subjects and was severe, resulting in recumbency, in the 2 mg kg?1 group. Respiratory rate and rectal temperature did not change significantly after injection of either treatment. Following epidural injection of 2 mg kg?1 of ketamine, heart rate increased significantly from the pre-injection baseline of 55 ± 2 to 76 ± 4 (mean ± SD) beats minute?1, but after the lower dose changes were not significant. The only significant changes in measured haematologic parameters were decreases in total erythrocyte count at 45 minutes and total leukocyte count from 45–75 minutes, in the 2 mg kg?1 group.ConclusionEpidural ketamine injection was associated with caudal anti-nociception, sedation and ataxia in the dromedary camels; the intensity and duration of which was dose dependant.Clinical relevanceNeither of the doses of epidural ketamine injection in our study was applicable for standing surgical procedures in dromedary camels.  相似文献   
8.
本文通过对聚合草单独青贮、聚合草+无芒雀麦(2∶1)混合青贮、聚合草+无芒雀麦(3∶1)混合青贮的结果,得出以聚合草+无芒雀麦(3∶1)最佳,聚合草单独青贮次之,聚合草+无芒雀麦(2∶1)最差。  相似文献   
9.
An effective technology of growing crops with tilling solonetz by a PMS-70 rototiller was developed. On the basis of a long-term field experiment, a 30-year period of influence of cyclic soil ameliorative farming practices of chestnut-solonetz complex soils of the Rostov oblast is substantiated.  相似文献   
10.

Background:

In gene therapy, the use of RNA molecules as therapeutic agents has shown advantages over plasmid DNA, including higher levels of safety. However, transient nature of RNA has been a major obstacle in application of RNA in gene therapy.

Methods:

Here, we used the internal ribosomal entry site of encephalomyocarditis virus and the 3’ non-translated region of Poliovirus to design an enterovirus-like RNA for the expression of a reporter gene (enhanced green fluorescent protein) and a suicide gene (thymidine kinase of herpes simplex virus). The expression of these genes was evaluated by flow cytometry and cytotoxicity assay in human colorectal adenocarcinoma cell line (SW480). We then armed RNA molecules with a target sequence for hsa-miR-143 to regulate their expression by microRNA (miRNA) mimics.

Results:

The results showed effective expression of both genes by Entrovirus-like RNA constructs. The data also showed that the restoration of hsa-miR-143 expression in SW480 leads to a significant translation repression of the introduced reporter and suicide genes.

Conclusion:

Collectively, our data suggest the potential use of Entrovirus-like RNA molecules in suicide gene therapy. Additionally, as a consequence of the possible downregulated miRNA expression in cancerous tissues, a decreased expression of gene therapy constructs armed with target sequences for such miRNA in cancer tissue is expected.Key Words: Thymidine kinase, Polio 3’NCR, miR-143  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号