Molecular markers based genetic relatedness studies in tissue culture propagated japanese plum cultivars Santa Rosa and Frontier     

Thakur  Manisha  Luharch  Rakshandha  Sharma  Vishal  Sharma  Dharam Paul 《Genetic Resources and Crop Evolution》2022,69(2):567-575

Genetic Resources and Crop Evolution - Santa Rosa and Frontier are the major Japanese plum (Prunus salicina Lindl.) cultivars grown throughout the world. The present investigation was performed to...  相似文献   

Identification of a diverse mini‐core panel of Indian rice germplasm based on genotyping using microsatellite markers          下载免费PDF全文

Kapil K. Tiwari  Anshuman Singh  Sasmita Pattnaik  Maninder Sandhu  Sukhdeep Kaur  Sourabh Jain  Sushma Tiwari  Shweta Mehrotra  Mahender Anumalla  Rashmita Samal  Jyoti Bhardwaj  Neha Dubey  Vikrant Sahu  Gayle A. Kharshing  Patu K. Zeliang  Kadiri Sreenivasan  Pankaj Kumar  Swarup K. Parida  Sevanthi V. A. Mithra  Vandana Rai  Wricha Tyagi  Pawan K. Agrawal  Atmakuri R. Rao  Arunava Pattanayak  Girish Chandel  Ashok K. Singh  Ishwari S. Bisht  Kangila V. Bhat  Gundimeda J. N. Rao  Jitendra P. Khurana  Nagendra K. Singh  Trilochan Mohapatra 《Plant Breeding》2015,134(2):164-171

Identification of a small core germplasm set representing the available genetic diversity is essential for its proper evaluation and subsequent utilization in rice improvement programmes. For constituting a small diverse mini‐core panel of Indian rice germplasm, a representative set of 6912 accessions drawn based on their geographic origin from the whole rice germplasm collection available in the National Gene Bank was genotyped using 36 microsatellite markers. Automated fragment analysis of amplicons yielded a total of 435 alleles, with an average 12.4 and range of 3–29 alleles per locus. Polymorphism information content (PIC) ranged from 0.08 (RGNMS190) to 0.86 (RM552) with an average of 0.528. Based on genotyping data, a mini‐core consisting of 98 genotypes was identified. Ninety‐four per cent of the alleles present in the core set were present in the mini‐core. The identified small but diverse panel will be useful for further intensive trait‐specific evaluation and utilization in allele mining.  相似文献   

Antiarthritic gold compounds effectively quench electronically excited singlet oxygen   总被引：5，自引：0，他引：5  

E J Corey  M M Mehrotra  A U Khan 《Science (New York, N.Y.)》1987,236(4797):68-69

Although certain gold [Au(I)] compounds have been used effectively in the treatment of rheumatoid arthritis for some years, the molecular basis for such therapeutic action has been unclear. One possible mechanism of the action of Au(I) compounds is that they protect unsaturated membrane lipids and proteins against oxidative degradation caused by activated phagocytes that are not properly regulated. In this study it has been shown that superoxide ion (O-2.), a product of activated phagocytes, can be oxidized to electronically excited singlet oxygen (O1(2)delta g), an agent that is capable of peroxidation of unsaturated fatty acid derivatives. It has also been shown that antiarthritic Au(I) compounds are effective deactivators of O1(2)delta g with quenching constants on the order of 10(7) M-1 sec-1.  相似文献   

Assessment of Atmospheric Arsenic Level in Airborne Dust Particulates of an Urban City of Central India     

Deb  Manas Kanti  Thakur  Manisha  Mishra  R. K.  Bodhankar  Ninad 《Water, air, and soil pollution》2002,140(1-4):57-71

An assessment of arsenic contamination in Raipur city (21°14′N, 18°38′E) of Chhattisgarh in the central part of India is reported here, for a monitoring period between November 1996 to June 1997, in airborne dust particulates. The concentration level of As were higher in the industrial site, followed by heavy traffic as compared to other sites. The monthly atmospheric arsenic deposition, in μg As per g of dust fall, of 6 sites are in the range of 0.100(μ0.020)–4.00(μ0.020); site no. 1 industrial area, 0.100(μ0.020)–0.320(μ0.020); site no. 2 residential area, 0.044(μ0.070)–0.337(μ0.030); site no. 3 commercial area, 0.093(μ0.068)–1.870(μ0.020); site no. 4 residential area, 0.111(μ0.020)–1.912(μ0.010); site no. 5 residential area and 0.068(μ0.040)–3.037(μ0.060); site no. 6 heavy traffic area. The total annual flux of As in the fall-out at different zones is in the range 0.033–1.12 kg km^-2 yr^-1. The month wise collection and analysis of dust fall out rate between 3.0(μ0.10)–91.3(μ1.4) mt (metric tonnes) km^-2 month^-1 were observed at all 6 sampling sites. Anthropogenic and environmental factors play important roles in the contribution of arsenic in airborne particulate matters.  相似文献   

Studies on the association of Aspergillus fumigatus with ocular infections in animals     

M Pal  B S Mehrotra 《The Veterinary record》1986,118(2):42-44

The occurrence and aetiological significance of Aspergillus fumigatus, an opportunistic pathogen, have been studied in 93 animals with various ophthalmological problems. A total of 93 eye swabs collected from 35 mules, 26 dogs, 13 fowl, 11 cattle, five buffaloes and three camels were investigated mycologically for the presence of A fumigatus. The pathogen was isolated in pure and heavy growth from the swabs from two dogs, one bull, one mule and one fowl. The fungus was also demonstrated directly in clinical material by the potassium hydroxide technique. A fumigatus could not be cultured from the buffaloes and camels. All the five cases had been treated with broad spectrum antibiotics and cortisone and two had received traumatic injury to the eyes (one mule and a bull). The organism was not isolated in pure culture from the conjunctival swabs of 22 apparently healthy animals (11 dogs, six mules, three fowl and two cattle). Many other saprophytic fungi were recovered in mixed cultures but were considered to be contaminants. The clinical signs and diagnostic criteria of oculomycosis have been discussed.  相似文献   

Transformation of Sucrose to Starch and Protein in Rice Leaves and Grains under Two Establishment Methods     

Kumari Manisha  Asthir Bavita 《水稻科学》2016,23(5):255

Six rice varieties, PR120, PR116, Feng Ai Zan, PR115, PAU201 and Punjab Mehak 1 were raised under aerobic and transplanting conditions to assess the effects of planting conditions on sucrose metabolising enzymes in relation to the transformation of free sugars to starch and protein in flag leaves and grains. Activities of sucrose synthase, sucrose phosphate synthase and acid invertase increased till flowering stage in leaves and mid-milky stage(14 d after flowering) in grains and thereafter declined in concomitant with the contents of reducing sugar. Under aerobic conditions, the activities of acid invertase and sucrose synthase(cleavage) significantly decreased in conjunction with the decrease in non-reducing sugars and starch content in all the varieties. Disruption of starch biosynthesis under the influence of aerobic conditions in both leaves and grains and the higher build up of sugars possibly resulted in their favoured utilization in nitrogen metabolism. Feng Ai Zan, PR115 and PR120 maintained higher levels of sucrose synthase enzymes in grains and leaves and contents of metabolites(amino acid, protein and non-reducing sugar) under aerobic conditions, while PR116, Punjab Mehak 1 and PAU201 performed better under transplanting conditions, thus showing their adaptation to environmental stress. Yield gap between aerobic and transplanting rice is attributed primarily to the difference in sink activity and strength. Overall, it appear that up-regulation of sucrose synthase(synthesis) and sucrose phosphate synthase under aerobic conditions might be responsible in enhancing growth and productivity of rice varieties.  相似文献   

Immune Responses and Protective Efficacy of Binary Ethylenimine (BEI)-Inactivated Bluetongue Virus Vaccines in Sheep   总被引：2，自引：0，他引：2  

Ramakrishnan MA  Pandey AB  Singh KP  Singh R  Nandi S  Mehrotra ML 《Veterinary research communications》2006,30(8):873-880

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Induction of novel variants through physical and chemical mutagenesis in Barbeton daisy (Gerbera jamesonii Hook.)     

Minerva Ghani  Surinder Kumar  Manisha Thakur 《The Journal of Horticultural Science and Biotechnology》2013,88(5):585-590

Summary

Gerbera (Gerbera jamesonii) is an attractive ornamental flower of high economic importance. The present investigation was aimed at generating novel flower colour variants in the gerbera cultivar ‘Harley’ through physical and chemical mutagenesis. In vitro-raised shoot cultures of gerbera, established from petiole explants, were exposed to different doses of γ-rays (1.5, 2.0, 2.5, 5.0, 10.0, 15.0, 20.0, or 30.0 Gy) using a Cobalt-60 source emitting 2.51 kGy h^–1. To induce mutations through chemical mutagenesis, different concentrations of ethylmethane sulphonate [EMS; 0.1, 0.2, 0.5, 0.8, or 1.0% (v/v)] were administered for 10 min or for 20 min. The LD50 values calculated for shoot survival and the induction of mutations were approx. 6.5 Gy for γ-rays and 0.65% (v/v) EMS for 10 min, or ≤ 0.1% (v/v) EMS for 20 min. Investigations revealed a negative correlation between mutagen dose and plant survival, both in vitro and after acclimatisation. Morphological variants showing changes in leaf shape, leaf size, scape length, flower diameter, and flower colour were obtained. Significantly, early flowering was induced in all mutated plants compared to non-mutated plants.The high frequencies of colour variants obtained using Bγ-rays, or the application of EMS to in vitro-raised shoot cultures could be an effective way to improve gerbera cultivars.  相似文献   

Standing in the canine precision medicine knowledge gap: Improving annotation of canine cancer genomic biomarkers through systematic comparative analysis of human cancer mutations in COSMIC     

Sharadha Sakthikumar  Salvatore Facista  Derick Whitley  Sara A. Byron  Zeeshan Ahmed  Manisha Warrier  Zhanyang Zhu  Esther Chon  Kathryn Banovich  David Haworth  William P. D. Hendricks  Guannan Wang 《Veterinary and comparative oncology》2023,21(3):482-491

The accrual of cancer mutation data and related functional and clinical associations have revolutionised human oncology, enabling the advancement of precision medicine and biomarker-guided clinical management. The catalogue of cancer mutations is also growing in canine cancers. However, without direct high-powered functional data in dogs, it remains challenging to interpret and utilise them in research and clinical settings. It is well-recognised that canine and human cancers share genetic, molecular and phenotypic similarities. Therefore, leveraging the massive wealth of human mutation data may help advance canine oncology. Here, we present a structured analysis of sequence conservation and conversion of human mutations to the canine genome through a ‘caninisation’ process. We applied this analysis to COSMIC, the Catalogue of Somatic Mutations in Cancer, the most prominent human cancer mutation database. For the project's initial phase, we focused on the subset of the COSMIC data corresponding to Cancer Gene Census (CGC) genes. A total of 670 canine orthologs were found for 721 CGC genes. In these genes, 365 K unique mutations across 160 tumour types were converted successfully to canine coordinates. We identified shared putative cancer-driving mutations, including pathogenic and hotspot mutations and mutations bearing similar biomarker associations with diagnostic, prognostic and therapeutic utility. Thus, this structured caninisation of human cancer mutations facilitates the interpretation and annotation of canine mutations and helps bridge the knowledge gap to enable canine precision medicine.  相似文献   

Pseudocercospora kaiseri sp. nov. causing a necrotic leaf spot of Erythrina     

M. D. Mehrotra 《Forest Pathology》1991,21(2):124-126

A new species, Pseudocercospora kaiseri, causing leaf spot of Erythrina stricta Roxb. from India is described and illustrated.  相似文献