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Comparison of flower color with anthocyanin composition patterns in evergreen azalea 总被引：2，自引：0，他引：2
Daiki Mizuta Takuya Ban Ikuo Miyajima Akira Nakatsuka Nobuo Kobayashi 《Scientia Horticulturae》2009,122(4):594-602
In evergreen azaleas, major anthocyanins were detected from petals of wild species and cultivars by HPLC analysis. Depending on flower color, all samples were divided into three groups: red, purple or white, using the Japan color standard for horticultural plants. The chromatic components a* and b* values of red group samples showed a convergent distribution, whereas those of purple group samples showed a wider distribution. According to the HPLC analysis, red group samples had two to four major anthocyanins, and those of the purple group had two to six major ones. In contrast, no anthocyanins were detected in the white group petals, although anthocyanidins were detected. These results suggest that the anthocyanin constitution of the purple group flowers is more varied than that of the red group flowers, and this wider variety among purple flowers contributes to extending the diversity of flower color in evergreen azalea. 相似文献
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Abe T Miyajima H Okada K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(9):985-988
A novel macrolide antibiotic was administered orally to 5-week-old Jcl:Wistar rats at a dose of 5,000 mg/kg/day for 5 weeks, and then a half of animals were maintained without any treatment for 10 weeks. A white discolored lesion with horizontal stripes developed on the surface of the upper and lower incisors after dosing for 4 weeks, and these macroscopical incisal lesions disappeared with the eruption in 4 weeks after stop of administration. Histopathologically, increase in number of karyopycnosis of ameloblast at the transitional stage, vacuolar degeneration of ameloblast and cystic change in the maturation stage, and impaired iron pigment secretion at the pigmentation stage were observed. Microradiography, calcio-traumatic zones, which means hypocalcification, were observed on the superficial layer of enamel. These results suggest that the primary lesion induced by a novel macrolide antibiotic is the increased karyopycnosis of ameloblast at the transitional stage, and followed by later stage. 相似文献
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Kirisawa R Kobayashi T Uematsu R Ikeda A Kuroiwa R Urakami A Iwai H 《Veterinary microbiology》2003,95(3):159-174
The relationship of passage-induced mutant genes 1 and 71 of an attenuated equine herpesvirus 1 (EHV-1) with virulence was analysed by constructing nine recombinant EHV-1 viruses by homologous recombination. Gene 1 or/and gene 71 of a virulent EHV-1 strain, HH1, was replaced by a mutant gene 1 or/and 71 of an attenuated HH1 strain, BK343, respectively. The beta-galactosidase gene of Escherichia coli was inserted within the gene 1 or 71 coding sequence of HH1 to inactivate the genes. Virus replications of these recombinant viruses in cell cultures were similar, but release of the gene 71-inactivated virus from infected cells was delayed compared to that of the other viruses. Plaque sizes of the recombinant viruses were similar to those of HH1, but those of BK343 were significantly smaller, indicating an effect of some unknown factor(s) on viral cell-to-cell spread. The growth abilities of the recombinant viruses with a mutant gene 1 or/and 71 in lungs of mice were similar to those of HH1, but those of gene 71-inactivated viruses were reduced to the level of BK343 and the titers were about 100-times lower than those of the other recombinant viruses. These results indicate that the mutant genes 1 and 71 of BK343 might not confer an attenuated nature to EHV-1. 相似文献
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Crozaz G Haack U Hair M Hoyt H Kardos J Maurette M Miyajima M Seitz M Sun S Walker R Wittels M Woolum D 《Science (New York, N.Y.)》1970,167(3918):563-566
Particle track densities up to > 3 x 10(9) per square centimeter have been measured in different samples. Rocks 17, 47, 57, and 58 have VH (Z >22) galactic cosmic ray ages of 11, 14, 28, and 13 x 10(6) years, respectively. Rock 57 has a calculated erosion rate of 10(-7) centimeter per year. Near-surface track versus depth data in rock 17 can be fit with solar flare particles that have a differential energy spectrum aE(-3); lunar samples can be used to study the history of solar activity. The uranium in the crystalline rocks occurs principally in small regions <10 to approximately 100 micrometers in size. The (low) thermoluminescence of the fines increases with depth in core 10004. With one possible exception, x-ray studies have not shown pronounced radiation damage effects. The total energy release upon heating is small up to 900 degrees C and occurs in three broad regions. 相似文献
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Y. Miyajima R. Masuda A. Kurihara R. Kamata Y. Yamashita T. Takeuchi 《Fisheries Science》2011,77(1):41-48
We conducted feeding experiments on threadsail filefish Stephanolepis cirrhifer juveniles for 16 days to evaluate the efficacy of moon jellyfish Aurelia aurita as a prey item. Four treatments, each with 40 individuals, were prepared to compare fish growth performance. The treatments
consisted of filefish that were starved (control) (S), fed only jellyfish (J), fed only krill (K), and fed both jellyfish
and krill (JK). Fish in the S treatment exhibited a 50% mortality rate and reduced body weight, whereas the J treatment exhibited
a zero mortality rate and increased body weight. Fish in the JK treatments showed a significantly faster growth than those
in the K treatment. Filefish consumed as much as 24 and 13 times their own body weight in jellyfish per day in the J and JK
treatments, respectively. This is the first report showing that growth can be sustained by feeding a marine fish only jellyfish,
and indicates the potential of jellyfish as a fish prey in both nature and captivity. 相似文献
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Satake S Nakamura C Minamide Y Kudo S Maeda H Chihaya Y Kamimura Y Miyajima H Sasaki J Goryo M Okada K 《Journal of toxicologic pathology》2010,23(2):75-83
To elucidate the effect of a large dose of di (2-ethylhexyl) phthalate (DEHP), a plasticizer and peroxisome proliferator-activated receptor-α (PPARα) agonist, on hepatic peroxisomes, we orally administered 1,000 mg/kg/day, once daily, to 3 male and 4 female cynomolgus monkeys for 28 days consecutively. Light-microscopic and electron microscopic examinations of the liver were carried out in conjunction with measurement of the hepatic fatty acid β-oxidation system (FAOS), carnitine acetyltransferase (CAT) and carnitine palmitoyltransferase (CPT) activities, which are peroxisomal and/or mitochondrial enzyme activities. Electron microscopically, enlargement of the mitochondria was observed with lamellar orientation of the cristae along the major axis. Although the number of peroxisomes showed a tendency to increase when compared with those in a biopsied specimen before treatment, no abnormality in morphology was observed. A slight increase in CPT activity was noted at termination. No changes were noted in hepatic FAOS or CAT activity. In conclusion, although repeated oral treatment of cynomolgus monkeys with a large dose of DEHP induced a subtle increase in the numbers of peroxisomes with slight enlargements of the mitochondria, this low-sensitivity response to peroxisome proliferators in cynomolgus monkeys was considered to be closer to the response in humans than that in rodents. 相似文献
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Arai Y Ohgane J Yagi S Ito R Iwasaki Y Saito K Akutsu K Takatori S Ishii R Hayashi R Izumi S Sugino N Kondo F Horie M Nakazawa H Makino T Shiota K 《The Journal of reproduction and development》2011,57(4):507-517
Epigenetic alteration is an emerging paradigm underlying the long-term effects of chemicals on gene functions. Various chemicals, including organophosphate insecticides and heavy metals, have been detected in the human fetal environment. Epigenetics by DNA methylation and histone modifications, through dynamic chromatin remodeling, is a mechanism for genome stability and gene functions. To investigate whether such environmental chemicals may cause epigenetic alterations, we studied the effects of selected chemicals on morphological changes in heterochromatin and DNA methylation status in mouse ES cells (ESCs). Twenty-five chemicals, including organophosphate insecticides, heavy metals and their metabolites, were assessed for their effect on the epigenetic status of mouse ESCs by monitoring heterochromatin stained with 4￠,6-diamino-2-phenylindole (DAPI). The cells were surveyed after 48 or 96 h of exposure to the chemicals at the serum concentrations of cord blood. The candidates for epigenetic mutagens were examined for the effect on DNA methylation at genic regions. Of the 25 chemicals, five chemicals (diethyl phosphate (DEP), mercury (Hg), cotinine, selenium (Se) and octachlorodipropyl ether (S-421)) caused alterations in nuclear staining, suggesting that they affected heterochromatin conditions. Hg and Se caused aberrant DNA methylation at gene loci. Furthermore, DEP at 0.1 ppb caused irreversible heterochromatin changes in ESCs, and DEP-, Hg- and S-421-exposed cells also exhibited impaired formation of the embryoid body (EB), which is an in vitro model for early embryos. We established a system for assessment of epigenetic mutagens. We identified environmental chemicals that could have effects on the human fetus epigenetic status. 相似文献
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Okano T Nakamura S Nakashita R Komatsu T Murase T Asano M Tsubota T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(10):1133-1137
The purpose of this study was to ascertain whether or not Japanese black bears (Ursus thibetanus japonicus) are induced ovulators. The progesterone levels of female bears kept with a male and allowed to mate (n=2) and female bears allowed contact with a male through bars but not allowed to mate (n=6) during the mating season increased significantly in late October. Based on this result, the female bears were considered to have ovulated. Females isolated from males (n=3) were ovarioectomized after the mating season, and no corpora lutea were observed, indicating they had not ovulated. These findings suggest that Japanese black bears may be induced ovulators that ovulate with stimuli from males and without coitus at a high rate. 相似文献