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排序方式: 共有173条查询结果,搜索用时 15 毫秒
1.
D. M. Zhao S. Tateyama N. Miyoshi K. Uchida R. Yamaguchi T. Yamagami T. Hayashi 《Research in veterinary science》1995,59(3)
Cloning of the canine yes oncogene was attempted from a c
library derived from a healthy canine spleen using a human c-yes-1 probe. The nucleotide and amino acid sequences revealed that the canine yes gene contained an open reading frame consisting of 539 amino acids. Its product had a molecular mass of 60,368 Daltons and showed 95·9 per cent and 90·4 per cent homology with human and chick p61c-yes, respectively. Moreover, the product had a myristylation signal, src homology region (SH) 3, SH2, and tyrosine kinase domains showing 98·8 per cent and 96·0 per cent homology with those of human beings and chickens, respectively. These findings indicate that the products of the canine yes gene may have non-receptor-type tyrosine kinase activity on the cell membrane, as is the case in human and chick p61c-yes 相似文献
2.
Hiroto Miura Takuya Hashimoto Yukiko Kawanishi Hiroki Kawauchi Ryo Inoue Noriaki Shoji Kunihiko Saito Mario Sekiya Yosuke Saito Jumpei Yasuda Chiemi Yonezawa Tetsushiro Endo Hirotaka Kasuya Yutaka Suzuki Yasuo Kobayashi Satoshi Koike 《Animal Science Journal》2021,92(1):e13601
The rumen microbiota comprises a vast range of bacterial taxa, which may affect the production of high-quality meat in Japanese Black cattle. The aim of this study was to identify core rumen microbiota in rumen fluid samples collected from 74 Japanese Black cattle raised under different dietary conditions using 16S rRNA gene amplicon sequencing. In the rumen of fattening Japanese Black cattle, 10 bacterial taxa, showing >1% average relative abundance and >95% prevalence, irrespective of the dietary conditions and the fattening periods, were identified as the core rumen bacterial taxa, which accounted for approximately 80% of the rumen microbiota in Japanese Black cattle. Additionally, population dynamics of the core rumen bacterial taxa revealed two distinct patterns: Prevotella spp. and unclassified Bacteroidales decreased in the mid-fattening period, whereas unclassified Clostridiales, unclassified Ruminococcaceae, Ruminococcus spp., and unclassified Christensenellaceae increased during the same period. Therefore, the present study reports the wide distribution of the core rumen bacterial taxa in Japanese Black cattle, and the complementary nature of the population dynamics of these core taxa, which may ensure stable rumen fermentation during the fattening period. 相似文献
3.
Murase T Imaeda N Yamada H Miyazawa K 《The Journal of reproduction and development》2007,53(4):853-865
This study attempted to explain the mechanisms regulating boar fertility by examining seasonal changes in semen characteristics, the composition of seminal plasma and responsiveness of sperm acrosomes to Ca(2+) and the Ca(2+) ionophore A23187 (Ca(2+)/A23187). Sperm-rich and sperm-poor fractions were separately collected from 3 mature fertile Large White boars once a month over a one-year period. During the period of study, ambient temperature and relative humidity were recorded for within the stall in which the boars were kept and the semen characteristics, composition of the seminal plasma of sperm-rich fractions, and occurrence of the acrosome reaction in response to Ca(2+) (3 mM)/A23187 (0.3 microM) were examined. The highest mean maximum and minimum ambient temperatures were recorded in August-September, whereas the lowest mean maximum and minimum ambient temperatures were recorded in December and January, respectively. There was a moderate peak in relative humidity from July to October. The lowest percentages of motile spermatozoa and of spermatozoa with intact acrosomes and highest percentage of spermatozoa with abnormal morphology and strongest agglutination were seen in August-September. The total protein and albumin concentrations were lowest in August-September. Testosterone levels increased gradually as day length decreased after the summer solstice (June) and peaked in October-November. The percentage of acrosome reactions in response to Ca(2+)/A23187 was highest with the quickest response in August-September, as shown by the shortest time required for 50% of relative acrosome reactions. The farrowing rates were lowest in these same 2 months. These results suggest that seasonal infertility in Large White boars may be due, at least in part, to a combination of low motility, abnormal morphology including acrosomal abnormality, and early occurrence of the acrosome reaction in response to stimulus, possibly resulting from a decrease in acrosomal stabilizing proteins in the seminal plasma during summer. These changes may be modulated by heat/humidity stress and/or photoperiod-regulated testosterone. 相似文献
4.
5.
Suppressive effect of liquid potassium silicate on powdery mildew of strawberry in soil 总被引:3,自引:0,他引:3
Takeshi Kanto Akihiro Miyoshi Takuya Ogawa Kazumasa Maekawa Masataka Aino 《Journal of General Plant Pathology》2006,72(3):137-142
A soil drench of soluble silicon (liquid potassium silicate) was tested for the control of powdery mildew of strawberry in
soil for 2 years in a vinyl house under growers' conditions on two cultivars: very susceptible Toyonoka and slightly susceptible
Sachinoka. Soluble silicon suppressed the disease more effectively as a preventive control than as a control to reduce initial
incidence. The suppressive effect against this disease was more effective for Toyonoka than for Sachinoka. The control value
of soluble potassium silicate was 85.6% for Toyonoka in the first year and 60.2% in the second year, while for Sachinoka it
was 58% in the first year and 40.6% in the second year. Strawberry leaf hardness was measured for the control and silicate-treated
leaves. Although silicate-treated leaves seemed to be harder than control leaves, leaf hardness did not vary significantly
between controls and silicate-treated leaves in either of the 2 years, and the mode of preventive action of liquid potassium
silicate remains unknown. A factor other than a physical action may be involved. 相似文献
6.
High neutrophil chemotactic activity was detected in the culture medium from Oesophagostomum aculeatum larvae in vitro using blind-well chambers with Millipore filters, and guinea pig leucocytes as indicator cells. Neutrophil chemotactic activity was also detected in the extract from larval worms in a dose dependent fashion. This activity was detected in the low molecular weight fractions adjacent to a sodium chloride marker by gel filtration on Sephadex G200. These results were further confirmed with monkey neutrophils. The possible role of this activity in the formation of granulomatous lesions rich in neutrophils found in O aculeatum infections in the Japanese monkey is discussed. 相似文献
7.
8.
Makiguchi Y Konno Y Konishi K Miyoshi K Sakashita T Nii H Nakao K Ueda H 《Fish physiology and biochemistry》2011,37(2):273-284
The movements of 28 adult chum salmon, Oncorhynchus keta (Walbaum) tagged with electromyogram (EMG) transmitters were tracked along the Toyohira river, Hokkaido, Japan, in October
of 2007 and 2008 to investigate and evaluate the upstream migratory behavior through the protection bed and fishway of ground
sills. The approach time of fish that ascended successfully through the protection bed and fishway was shorter than that of
unsuccessful fish. The unsuccessful fish were observed to swim in currents with high water velocity and shallow water depth
at swimming speeds that exceeded their critical swimming speed (U
crit) during the approach to these structures. In consequence, unsuccessful fish frequently alternated between burst and maximum
sustained speeds without ever ascending the fishway, and eventually became exhausted. It is important that fishway are constructed
to enable chum salmon to find a passage way easily, so that they can migrate upstream rapidly without wasting excessive energy. 相似文献
9.
This study was carried out to investigate the distribution of immune cells in the bovine placenta during the postpartum period and to compare these cells between normal and retained placenta. Within 1 h after normal calving, biopsy samples of placentomes were collected from 10 cows. The occurrence of retention of fetal membranes was monitored for more than 8 h post-calving, and the samples obtained were divided into two groups: normally discharged and retained placenta (n = 5 each). Immunohistochemical procedures were utilized to detect macrophages and T lymphocytes. Numerous CD14-positive macrophages were found in the stroma of both normal placenta and retained placenta whereas only a few CD3-positive T lymphocytes were found in both cases. However, histochemical staining for acid phosphatase, a predominant lysozomal enzyme, revealed that almost all macrophages showed strong enzyme activity in the normally discharged placentas, whereas in retained placenta the activity of acid phosphatase was conspicuously decreased in intensity. These results indicate that there are functional differences in placental macrophages between normal and retained placenta. 相似文献
10.
Yankovskaya V Horsefield R Törnroth S Luna-Chavez C Miyoshi H Léger C Byrne B Cecchini G Iwata S 《Science (New York, N.Y.)》2003,299(5607):700-704
The structure of Escherichia coli succinate dehydrogenase (SQR), analogous to the mitochondrial respiratory complex II, has been determined, revealing the electron transport pathway from the electron donor, succinate, to the terminal electron acceptor, ubiquinone. It was found that the SQR redox centers are arranged in a manner that aids the prevention of reactive oxygen species (ROS) formation at the flavin adenine dinucleotide. This is likely to be the main reason SQR is expressed during aerobic respiration rather than the related enzyme fumarate reductase, which produces high levels of ROS. Furthermore, symptoms of genetic disorders associated with mitochondrial SQR mutations may be a result of ROS formation resulting from impaired electron transport in the enzyme. 相似文献