Pharmacokinetic assessment of the monepantel plus oxfendazole combined administration in dairy cows          下载免费PDF全文

M. Ballent  P. Viviani  F. Imperiale  P. Dominguez  S. Halwachs  H. Mahnke  W. Honscha  C. Lanusse  G. Virkel  A. Lifschitz 《Journal of veterinary pharmacology and therapeutics》2018,41(2):292-300

Monepantel (MNP) is a novel anthelmintic compound launched into the veterinary pharmaceutical market. MNP is not licenced for use in dairy animals due to the prolonged elimination of its metabolite monepantel sulphone (MNPSO₂) into milk. The goal of this study was to evaluate the presence of potential in vivo drug‐drug interactions affecting the pattern of milk excretion after the coadministration of the anthelmintics MNP and oxfendazole (OFZ) to lactating dairy cows. The concentrations of both parent drugs and their metabolites were measured in plasma and milk samples by HPLC. MNPSO₂ was the main metabolite recovered from plasma and milk after oral administration of MNP. A high distribution of MNPSO₂ into milk was observed. The milk‐to‐plasma ratio (M/P ratio) for this metabolite was equal to 6.75. Conversely, the M/P ratio of OFZ was 1.26. Plasma concentration profiles of MNP and MNPSO₂ were not modified in the presence of OFZ. The pattern of MNPSO₂ excretion into milk was also unchanged in animals receiving MNP plus OFZ. The percentage of the total administered dose recovered from milk was 0.09 ± 0.04% (MNP) and 2.79 ± 1.54% (MNPSO₂) after the administration of MNP alone and 0.06 ± 0.04% (MNP) and 2.34 ± 1.38% (MNPSO₂) after the combined treatment. The presence of MNP did not alter the plasma and milk disposition kinetics of OFZ. The concentrations of the metabolite fenbendazole sulphone tended to be slightly higher in the coadministered group. Although from a pharmacodynamic point of view the coadministration of MNP and OFZ may be a useful tool, the presence of OFZ did not modify the in vivo pharmacokinetic behaviour of MNP and therefore did not result in reduced milk concentrations of MNPSO₂.  相似文献   

Effects of additive iron on growth,tissue distribution,haematology and immunology of gilthead sea bream, <Emphasis Type="Italic">Sparus aurata</Emphasis>     

George Rigos  Alexandros Samartzis  Morgane Henry  Eleni Fountoulaki  Efthimia Cotou  John Sweetman  Simon Davies  Ioannis Nengas 《Aquaculture International》2010,18(6):1093-1104

The comparative effects of iron-supplemented levels on growth, tissue distribution, haematology and immunology of gilthead sea bream, Sparus aurata (2 g) were investigated, using four organic (50, 100, 200, 300 mg ORG/kg diet) and one inorganic iron source (200 INOR mg/kg diet). Fish were treated for 12 weeks with the experimental diets and maintained at a water temperature of 19–22°C. Growth (final weight and specific growth rate), tissue distribution (spleen, liver and muscle), haematological parameters (red blood cells, haematocrit, haemoglobin and mean corpuscular haemoglobin concentration) and non-specific immune indexes (respiratory burst activity and antibacterial activity of serum) were analysed. No significant differences were found in growth and iron tissue distribution among the tested groups. Red blood cell counting was statistically higher in fish given 50 ORG, 100 ORG, 200 ORG and 200 INOR feeds. However, haematocrit, haemoglobin and mean corpuscular haemoglobin concentration were not significantly affected by increasing dietary iron. Fish receiving the 100 ORG diet had the best performance with respect to the respiratory burst activity and significantly higher values for antibacterial activity of serum were obtained in fish fed with the 300 ORG diet. The present findings provided no clear evidence of the optimum iron concentration. However, there was adequate indication that iron supplementation enhanced the performance of gilthead sea bream, mainly from a haematological and immunological point of view.  相似文献   

Analyses of monoclonal antibodies reacting with porcine wCD6: Results from the Second International Swine CD workshop     

M. D. Pescovitz  B. K. Book  B. Aasted  J. Dominguez  R. Bullido  I. Trebichavsky  B. Novikov  I. Valpotic  J. Nielsen  S. Arn  D. H. Sachs  J. K. Lunney  P. C. Boyd  J. Walker  R. Lee  N. Petrinec  A. Saalmüller 《Veterinary immunology and immunopathology》1998,60(3-4):285-289

Among the 57 monoclonal antibodies analyzed within the T-cell group of the Second International Swine CD Workshop, one mAb fell within cluster T14a that included the CD6 standard a38b2 (No. 175). The new mAb MIL8 (No. 082) and a38b2 both precipitated from activated T-cells a 150 kDa monomeric protein. Staining patterns on the various cell types were similar. There was no inhibition of binding of either mAb to peripheral blood T-cells with the opposite mAb. The new mAb, MIL8, reacts with a separate epitope on porcine wCD6.  相似文献   

Report on the analyses of mAb reactive with porcine CD8 for the second international swine CD workshop     

F. A. Zuckermann  M. D. Pescovitz  B. Aasted  J. Dominguez  I. Trebichavsky  B. Novikov  I. Valpotic  J. Nielsen  S. Arn  D. H. Sachs  J. K. Lunney  P. Boyd  J. Walker  R. Lee  W. C. Davis  I. R. Barbosa  A. Saalmü  ller 《Veterinary immunology and immunopathology》1998,60(3-4):291-303

Based on an analysis of their reactivity with porcine peripheral blood lymphocytes (PBL), only three of the 57 mAbs assigned to the T cell/activation marker group were grouped into cluster T9 along with the two wCD8 workshop standard mAbs 76-2-11 (CD8a) and 11/295/33 (CD8b). Their placement was verified through the use of two-color cytofluorometry which established that all three mAbs (STH101, #090; UCP1H12-2, #139; and PG164A, #051) bind exclusively to CD8⁺ cells. Moreover, like the CD8 standard mAbs, these three mAbs reacted with two proteins with a MW of 33 and 35 kDa from lymphocyte lysates and were, thus, given the wCD8 designation. Because the mAb STH101 inhibited the binding of mAb 76-2-11 but not of 11/295/33, it was given the wCD8a designation. The reactivity of the other two new mAbs in the T9 cluster with the various subsets of CD8⁺ lymphocytes were distinct from that of the other members in this cluster including the standards. Although the characteristic porcine CD8 staining pattern consisting of CD8^low and CD8^high cells was obtained with the mAb UCP1H12-2, a wider gap between the fluorescence intensity of the CD8^low and CD8^high lymphocytes was observed. In contrast, the mAb PG164A, not only exclusively reacted with CD4⁻/CD8^high lymphocytes, but it also failed to recognize CD4/CD8 double positive lymphocytes. It was concluded that this mAb is specific for a previously unrecognized CD8 epitope, and was, thus, given the wCD8c designation. A very similar reactivity pattern to that of PG164A was observed for two other mAbs (STH106, #094; and SwNL554.1, #009). Although these two mAbs were not originally positioned in the T cell subgroup because of their reactivity and their ability to inhibit the binding of PG164A, they were given the wCD8c designation. Overall, five new wCD8 mAbs were identified. Although the molecular basis for the differences in PBL recognition by these mAbs is not yet understood, they will be important in defining the role of CD8⁺ lymphocyte subsets in health and disease.  相似文献   

Analyses of monoclonal antibodies reacting with porcine CD3: results from the Second International Swine CD Workshop   总被引：2，自引：0，他引：2  

M. D. Pescovitz  B. K. Book  B. Aasted  J. Dominguez  A. Ezquerra  I. Trebichavsky  B. Novikov  I. Valpotic  J. Nielsen  S. Arn  D. H. Sachs  J. K. Lunney  P. C. Boyd  J. Walker  R. Lee  G. Lackovic  P. Kirkham  R. M. E. Parkhouse  A. Saalmü  ller 《Veterinary immunology and immunopathology》1998,60(3-4):261-268

Among the 57 monoclonal antibodies (mAb) analyzed within the T-cell group from the Second Swine CD Workshop, six mAb fell within clusters T10 and T11 (No. 088, STH164; No. 148, FY1A3; No. 149, FY2C1; No. 150, FY1H2; No. 151, FY2A11; No. 169, BB23-8E6). The mAb within these two groups gave a similar appearance on flow cytometry and stained all peripheral blood T-cells as defined by CD4 and wCD8 staining. All six mAb precipitated a 24 kDa protein. On the basis of inhibition analyses performed as part of the workshop and from published data, the mAb define at least three epitopes. There is only minimal stimulation of resting peripheral lymphocytes, but four of the mAb produce strong stimulation in the presence of PMA. With the exception of STH164, all have been shown to react with CD3-transfected COS cells. The new mAb, therefore, react with three epitopes on porcine CD3 designated CD3a (BB23-8E6, FY2A11), CD3b (FY1A3, FY2C1), and CD3c (FY1H2). mAb STH164 appears to be reactive with another epitope, however, since its reactivity with CD3 has not been confirmed it is designated as wCD3.  相似文献   

Hepatitis E virus chronic infection of swine co-infected with Porcine Reproductive and Respiratory Syndrome Virus     

Morgane Salines  Elodie Barnaud  Mathieu Andraud  Florent Eono  Patricia Renson  Olivier Bourry  Nicole Pavio  Nicolas Rose 《Veterinary research》2015,46(1)

In developed countries, most of hepatitis E human cases are of zoonotic origin. Swine is a major hepatitis E virus (HEV) reservoir and foodborne transmissions after pork product consumption have been described. The risk for HEV-containing pig livers at slaughter time is related to the age at infection and to the virus shedding duration. Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) is a virus that impairs the immune response; it is highly prevalent in pig production areas and suspected to influence HEV infection dynamics. The impact of PRRSV on the features of HEV infections was studied through an experimental HEV/PRRSV co-infection of specific-pathogen-free (SPF) pigs. The follow-up of the co-infected animals showed that HEV shedding was delayed by a factor of 1.9 in co-infected pigs compared to HEV-only infected pigs and specific immune response was delayed by a factor of 1.6. HEV shedding was significantly increased with co-infection and dramatically extended (48.6 versus 9.7 days for HEV only). The long-term HEV shedding was significantly correlated with the delayed humoral response in co-infected pigs. Direct transmission rate was estimated to be 4.7 times higher in case of co-infection than in HEV only infected pigs (0.70 and 0.15 per day respectively). HEV infection susceptibility was increased by a factor of 3.3, showing the major impact of PRRSV infection on HEV dynamics. Finally, HEV/PRRSV co-infection – frequently observed in pig herds – may lead to chronic HEV infection which may dramatically increase the risk of pig livers containing HEV at slaughter time.

Electronic supplementary material

The online version of this article (doi:10.1186/s13567-015-0207-y) contains supplementary material, which is available to authorized users.  相似文献   

Use of the Single Seed Descent Method in Breeding Safflower (Carthamus tinctorius L.)     

J. Fernandez Martinez  J. Dominguez Gimenez  A. Jimenez  L. Hernandez 《Plant Breeding》1986,97(4):364-367

The single seed descent (SSD) method of selection has been evaluated in safflower (Carthamus tinctorius L.). The initial population of this study was a BC₂F₁ from a cross between a well adapted and a high oil cultivar. Transgressive segregation for yield and oil content producing lines with considerably higher values than the better parent was observed in BC₂F₄ and BC₂F₅ generations. These results and the great saving of time and effort makes the SSD method a very desirable tool for safflower breeders.  相似文献   

Screening for resistance to broomrape (Orobanche cernua) in cultivated sunflower     

J. Dominguez    J. M. Melero-Vara  J. Miller  J. M. Fernández-Martinez 《Plant Breeding》1996,115(3):201-202

Racial evolution of sunflower broomrape (Orobanche cernua) has been very rapid in Spain during recent years, in which resistance has been overcome several times and there has been an important increase in areas infested with this parasitic angiosperm. In order to find resistance to a highly virulent population of sunflower broomrape that could be used directly in breeding programmes, three different sets of cultivated plant material composed of 429 entries were tested by artificial inoculation. All evaluated inbred lines from Moden, Canada, were fully susceptible. Out of the 240 P.I. accessions tested, only 10 segregated for resistance to broomrape, the rest being susceptible. From the 160 USDA breeding lines evaluated, 5% were resistant and 19% segregated for resistance to O. cernua. These lines traced back mainly to crosses of RHA 274 and RHA 801 with Russian, Turkish and Romanian hybrids. The origin of P.I. accessions that segregated for resistance were primarily derived from the former USSR and from Romania.  相似文献   

NOTE ON ARTERIOSCLEROSIS IN RABBITS CAUSED BY SOME SAMPLES OF URANIUM NITRATE     

Dominguez R 《Science (New York, N.Y.)》1926,64(1660):407-408

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Rete mirabile of dolphin: its pressure-damping effect on cerebral circulation     

E L Nagel  P J Morgane  W L McFarlane  R E Galliano 《Science (New York, N.Y.)》1968,161(844):898-900

In the bottlenose dolphin Tursiops truncatus, a massive retial complex is interposed between the systemic and cerebral circulations at the cervicothoracic level. Pressure measurements in the retial efferent arteries supplying the brain revealed relatively nonpulsatile pressure profiles. These measurements in the anesthetized dolphin demonstrate the pressure-damping effect of the retia mirabile.  相似文献