Effects of dietary corticosterone and trilostane on growth and skeletal muscle protein turnover in broiler cockerels     

K. Hayashi  Y. Nagai  A. Ohtsuka  Y. Tomita 《British poultry science》1994,35(5):789-798

1. The effects of dietary corticosterone and trilostane, an inhibitor of glucocorticoid synthesis, on: growth, rates of synthesis and breakdown of skeletal muscle protein, and content of abdominal fat were studied in broiler chickens.

2. Dietary corticosterone (5, 10 or 20 mg/kg) depressed body weight gain and increased abdominal fat content in a dose‐dependent manner while dietary trilostane (1.4 or 7.0 mg/kg) had no effect.

3. The rate of protein breakdown in skeletal muscle estimated from N‐methylhistidine excretion was increased in a dose‐dependent manner by dietary corticosterone but it was decreased by trilostane.

4. The rate of skeletal muscle protein synthesis was not affected by corticosterone although it was decreased by trilostane.

5. Plasma corticosterone concentration was increased in a dose‐dependent manner by dietary corticosterone and decreased by treatment with 7 mg trilostane/kg diet.

6. The results indicate that higher concentrations of plasma corticosterone increase protein breakdown in skeletal muscle but do not affect muscle protein synthesis while both the rates of synthesis and breakdown are decreased when plasma corticosterone concentration is reduced.  相似文献   

Spermatic and oxidative profile of domestic cat (Felis catus) epididymal sperm subjected to different cooling times (24, 48 and 72 hours)          下载免费PDF全文

DSR Angrimani  KK Nagai  BR Rui  LC Bicudo  JDA Losano  MM Brito  MCP Francischini  M Nichi 《Reproduction in domestic animals》2018,53(1):163-170

Cooling stored epididymal samples for several days allows facilities to transport and process genetic material post‐mortem. Improvements to this practice allow the preservation of sperm from domestic cats, which are the ideal study model for wild felids. However, the modifications in spermatic features and the oxidative profile are not fully understood in cats. This information is necessary for the development of biotechniques, such as new extenders for cryopreservation. Therefore, the purpose of this study was to evaluate the spermatic and oxidative profile in samples from the epididymal cauda of domestic cats cooled at 5°C for 24, 48 and 72 hr. Spermatozoa were collected from the epididymis cauda. Evaluations consisted of computer‐assisted sperm analysis (CASA), plasma membrane integrity (eosin/nigrosin), acrosome integrity (fast green/rose bengal), sperm morphology, sperm DNA integrity (toluidine blue), mitochondrial activity (3′3 diaminobenzidine), activity of the antioxidant enzymes glutathione peroxidase (GPx) and superoxide dismutase (SOD), measurement of lipid peroxidation (TBARS) and protein oxidation. A decrease in sperm motility parameters was observed after 72 hr of cooling (i.e. total and progressive) with a higher percentage of minor (37.7 ± 6.3%) and total defects (53.4 ± 6.3%). Additionally, a decrease in high mitochondrial activity (Class I: 16.6 ± 2.2%) occurred after 72 hr. The decrease in motility rates after a long cooling time probably was caused by the increase in sperm abnormalities. A long cooling time causes cold shock and mitochondrial exhaustion, but there was no observed change with the oxidative stress condition. Therefore, cat epididymal sperm stored at 5°C appear to maintain a high quality for up to 48 hr of cooling time.  相似文献   

Outbreak of diarrhoea and milk drop in cows infected with bovine group B rotavirus     

Hayashi M  Nagai M  Hayakawa Y  Takeuchi K  Tsunemitsu H 《The Veterinary record》2001,149(11):331-332

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Antifungals produced by dinoflagellates—gambieric acids     

Hiroshi Nagai  Yuzuru Mikami  Takeshi Yasumoto 《Pest management science》1995,44(1):92-94

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Immunohistochemical analysis of pseudorabies virus spread through neurons innervating the eyeball     

Hiromi Takahashi  Chieko Kai  Yasuhiro Yoshikawa  Kazuya Yamanouchi 《Comparative immunology, microbiology and infectious diseases》1995,18(4):275-281

Pseudorabies virus (PRV) was inoculated intraocularly into BALB/c mice, and the distribution pattern of cells positive for several neurotransmitters and viral antigens in the eyeball, trigeminal nerve ganglia, and superior cervical ganglia was examined immunohistochemically to clarify the neural route of the virus spread. In the eyeball, substance P (SP)- and calcitonin gene-related peptide (CGRP)-positive cells were detected in the ipsilateral iris and ciliary body, neuropeptide tyrosine (NPY)-positive cells were detected in the choloid membrane, and tyrosine hydroxylase (TH)-positive cells were detected in the ipsilateral inner nuclear layer of the retina; all these cells contained viral antigens. In the superior cervical ganglia, viral antigen-positive cells containing TH or NPY were found at bilateral sites. In the trigeminal nerve ganglia, viral antigen-positive cells containing SP or CGRP were found at bilateral sites. These findings indicated that the SP- and CGRP-positive ganglion cells of the trigeminal nerve ganglia innervating the iris or ciliary body, and the NPY-positive ganglion cells of the superior cervical ganglia innervating the iris, ciliary body, and choroid membrane served as the route for the virus spread. These findings also suggested that dopaminergic neurons, such as the TH-positive retinal cells and TH-positive ganglion cells of the superior cervical ganglia, served as the route for virus spread.  相似文献   

Molecular mechanisms underlying pig oocyte maturation and fertilization   总被引：2，自引：0，他引：2  

Sun QY  Nagai T 《The Journal of reproduction and development》2003,49(5):347-359

Since the pig is not only an important farm animal, but also a model animal for biomedical applications, the development of reproductive technologies in this species has been very important. In vitro oocyte maturation and fertilization (IVM-IVF) are basic techniques for a number of oocyte- or embryo-related technologies. The practical aspects for pig oocyte IVM-IVF have been reviewed, while the molecular mechanisms underlying oocyte meiotic maturation and fertilization have not been well summarized, although accumulating data have been obtained in recent one decade. This review will focus on what is known about the molecular mechanisms of porcine oocyte maturation and fertilization such as first meiosis resumption, meiotic spindle assembly, second meiosis metaphase (MII) arrest during oocyte maturation, sperm-egg recognition and fusion, sperm acrosome reaction, second meiosis resumption, sperm chromatin decondensation, and pronucleus formation during fertilization, as well as the establishment of polyspermy block.  相似文献   

Intracytoplasmic sperm injection in the pig: where is the problem?   总被引：1，自引：0，他引：1  

Kren R  Kikuchi K  Nakai M  Miyano T  Ogushi S  Nagai T  Suzuki S  Fulka J  Fulka J 《The Journal of reproduction and development》2003,49(4):271-273

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Cytotoxicity for porcine islet cells by complement of six animal species     

Itakura S  Kato H  Wang PC  Matsunaga A  Jitsukawa T  Edamura K  Ohgawara H  Mochizuki M  Nishimura R  Sasaki N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(10):1107-1109

Complement-mediated cytotoxicity for porcine islet cells (PICs) was evaluated using sera of six animal species. Then soluble complement receptor type-1 (sCR1) as an anti-complement agent was added to those sera, and the changes in 50% hemolytic unit of complement serum (CH50) and cytotoxic effect of those sera on PICs were examined. All the sera except for that of pig showed cytotoxicity. However, the extent of toxicity was considerably different between species. In the rat and human serum, sCR1 significantly reduced CH50 and cytotoxicity, however in the dog serum, sCR1 had no suppressive effects. These results may suggest that complement contribute to humoral cytotoxicity for PICs as a main factor, and the compatibility of complement with PICs differs between animal species.  相似文献   

Langerhans cells within the follicular epithelium and the intradermal sweat duct in equine insect hypersensitivity "Kasen"     

Kurotaki T  Narayama K  Arai Y  Arai S  Oyamada T  Yoshikawa H  Yoshikawa T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(6):539-541

Histopathologic and electron microscopic observations were given on Langerhans cells (LCs) within the follicular epithelium (FE) and intradermal sweat duct (ISD) of equine "Kasen". By light microscopy, LCs were present in the greatest numbers within the FE and ISD than within the epidermal layer and the normal skin, with an occasional formation of several aggregated foci. By electron microscopy, LCs within the FE and ISD widely extended their dendritic processes between the keratinocytes and contained Birbeck granules (Bgs), mitochondria, rough endoplasmic reticula and ribosomes in the cytoplasm. Numerous Type 2 LCs, with a number of Bgs and endocytosis, and Type 3 LCs, with multivesicular bodies and endosomes of various sizes, were recognized within the FE and ISD, although inactive Type 1 LCs, with a narrow and lucid cytoplasm, were rarely seen. LCs observed within the FE and ISD in the "Kasen" skin lesions might express the particular stage corresponded to recognize, intake and process the antigens which permeate them.  相似文献   

Nucleotide sequence homology to bovine viral diarrhea virus 2 (BVDV 2) in the 5′ untranslated region of BVDVs from cattle with mucosal disease or persistent infection in Japan     

M. Nagai  M. Sato  H. Nagano  H. Pang  X. Kong  T. Murakami  T. Ozawa  H. Akashi 《Veterinary microbiology》1998,60(2-4):271-276

Cytopathogenic and non-cytopathogenic bovine viral diarrhea viruses (BVDVs) were isolated from cattle with mucosal disease or persistent infection in Japan. These isolates were compared for antigenic properties by cross-neutralization tests with Japanese reference strains of BVDV belonging to classical type 1. Significantly low cross-reactivity to reference strains was noted, indicating the viruses to possibly represent a new serotype in Japan. Thus, to determine the genotype of the isolates, nucleotide sequences of the 5′ untranslated region were determined and compared with those of previously reported BVDV 1 and 2. The isolates were clearly shown to belong to BVDV 2, not to BVDV 1.  相似文献