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从采自新疆乌鲁木齐市周边的5个奶牛场疑患隐性乳腺炎奶牛乳样,分离、纯化、鉴定出34株牛乳源大肠杆菌.根据GenBank发表的大肠杆菌16SrRNA序列和eaeA基因序列,用Oligo 6.0软件分别设计两对特异性引物,对分离菌株进行复检,并利用聚合酶链式反应(PCR)对eaeA基因进行扩增,结果10株为阳性,阳性率为2... 相似文献
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库尔勒香梨花序、序位和气温对座果率的影响 总被引：1，自引：0，他引：1
通过对库尔勒香梨（简称香梨）不同序数、同一花序不同序位以及花期气温对座果率影响的调查分析，发现香梨的同一花序随着序位的升高座果率逐渐下降。在对香梨疏花时，初果期的幼树留取花序基部1，2，3序位的花朵，结果盛期的成年树序数少的花序保留基部1，2，3序位的花朵，序数多的留取基部1～4序位或1～5序位的花朵为最佳留花位置。 相似文献
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通过制配高抗硫酸盐硅酸盐水泥胶砂试件,在不同浓度的硫酸盐溶液中进行长龄期的侵蚀试验,并和掺粉煤灰普通水泥胶砂试件进行对比侵蚀实验。采用扫描电镜(SEM)、能谱(EDS)观测分析试件微观特征。结果表明,在充分考虑侵蚀时间效应的情况下,高抗硫酸盐硅酸盐水泥胶砂试件难以抵抗高浓度的硫酸盐侵蚀,也难以抵抗较低浓度硫酸盐的长期侵蚀;高抗硫酸盐硅酸盐水泥胶砂试件与掺粉煤灰的普通水泥胶砂试件的抗硫酸盐侵蚀能力存在显著差异。 相似文献
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[目的]研究不同配比棉花秸秆复合基质对水果黄瓜幼苗生长的影响.[方法]通过在棉花秸秆中分别加入尿素、磷酸二铵和清水,进行腐熟发酵,再将各处理腐熟的棉花秸秆与草炭、蛭石、珍珠岩按不同比例配成复合基质,以水果黄瓜为试验材料进行育苗试验.[结果]不同配比复合基质对水果黄瓜幼苗的生长影响较大.以棉花秸秆(磷酸二铵处理)∶蛭石∶草炭=2∶1∶1配比的复合基质效果最好,其容重为0.27 g/cm3,总孔隙度为84.72;,通气孔隙为26.34;,持水孔隙为58.38;,pH为6.88,EC值为1.11 ms/cm;水果黄瓜幼苗生长势良好,叶绿素含量较高,育苗效果理想.纯棉花秸秆配方的育苗效果最差.[结论]棉花秸秆(磷酸二铵处理)∶蛭石∶草炭=2∶1∶1可以推荐作为水果黄瓜育苗基质. 相似文献
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Rivas S Gullón B Gullón P Alonso JL Parajó JC 《Journal of agricultural and food chemistry》2012,60(17):4296-4305
Pinus pinaster wood samples were subjected to double hydrothermal processing. The liquors coming from the second stage, containing soluble saccharides of polymeric or oligomeric nature from hemicelluloses (POHs), were subjected to membrane processing (operating in discontinuous diafiltration) for refining and fractionation. Refined POH fractions were characterized by matrix-assisted laser desorption/ionization time of flight mass spectrometry and chromatographic techniques. The most complex POH component was made up of 14 hexoses and contained 4 acetyl groups. The fermentability of purified POHs by human fecal inocula was assessed by measuring both carbon source consumption and formation of short-chain fatty acids. The bifidogenic ability of POHs was confirmed by fluorescence in situ hybridization. The stimulatory effects on the bifidobacterial population reached by POHs were of the same order as those obtained with commercial fructooligosaccharides. 相似文献
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Traceability is of particular importance for those persons who suffer allergy or intolerance to some food component(s) and need a strict avoidance of the allergenic food. In this paper, methodologies are described to fingerprint the presence of allergenic species such as carrot, tomato, and celery by DNA detection. Three DNA extraction methods were applied on vegetables and foods containing or not containing the allergens, and the results were compared and discussed. Fast SYBR Green DNA melting curve temperature analyses and duplex PCR assays with internal control have been developed for detection of these allergenic vegetables and have been tested on commercial foods. Spiking food experiments were also performed, assessing that limits of detection (LOD) of 1 mg/kg for carrot and tomato DNA and 10 mg/kg for celery DNA have been reached. 相似文献
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Objectives : To establish the mode of inheritance and describe the clinical features of epilepsy in the Belgian shepherd, taking the outset in an extended Danish dog family (199 individuals) of Groenendael and Tervueren with accumulated epilepsy. Methods : Epilepsy positive individuals (living and deceased) were ascertained through a telephone interview using a standardised questionnaire regarding seizure history and phenomenology. Living dogs were invited to a detailed clinical evaluation. Litters more than five years of age, or where epilepsy was present in all offspring before the age of five, were included in the calculations of inheritance. Results : Out of 199 family members, 66 dogs suffered from epilepsy. The prevalence of epilepsy in the family was 33%. Fifty‐five dogs experienced focal seizures with or without secondary generalisation, while four dogs experienced primary generalised seizures. In seven dogs, seizures could not be classified. The mode of inheritance of epilepsy was simple Mendelian. Clinical Significance : This study identified that the Belgian shepherd suffers from genetically transmitted focal epilepsy. The seizure phenomenology expressed by family members have a strong resemblance to what has been reported for familial partial (focal) epilepsy in humans with variable foci with suggestion of linkage to chromosome 2 and chromosome 22q12. 相似文献