Hemorrhagic necrotizing splenitis in a slaughter pig infected with Arcanobacterium species     

Ohba T  Shibahara T  Kobayashi H  Kubo M  Takashima A  Imai S  Murakami S  Kadota K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(4):449-453

A 6-month-old barrow presented with lethargy, inappetence and dysstasia. At necropsy, multiple coalescing hemorrhagic foci were detected in the margins of the spleen. Gram-positive bacilli were isolated from the spleen, kidney, muscle and liver. Comparative 16S rDNA gene sequencing analysis of the isolates (TO16177) revealed that they would be the same species of unpublished Arcanobacterium species strain HJ57-14E (accession no. gi 18873551) (99.7% similarity based on a comparison of 675 bp). Histologic examination of the splenic tissue sections revealed extensive necrosis and inflammation, and gram-positive bacilli were discernible. Multifocal necrosis was also detected in the liver. Immunohistochemically, the isolates were cross-reacted with polyclonal antibodies against Arcanobacterium pyogenes and Actinomyces naeslundii, and the reaction was strong for the latter. Similar reactions were found in the suppurative lesions of the tonsil, and occasionally in the spleen and lymph nodes. The present results indicate that the unpublished Arcanobacterium species induced multiple organ failure accompanied by acute hemorrhagic necrotizing splenitis in this growing-finishing pig.  相似文献   

Lesion-based analysis of leaf blast suppression in mixture of rice cultivar and a resistant near-isogenic line     

Taketo Ashizawa  Masashi Sasahara  Atsushi Ohba  Takeshi Hori  Kouji Ishikawa  Yukio Sasaki  Tomohisa Kuroda  Ryoei Harasawa  Kaoru Zenbayashi-Sawata  Shinzo Koizumi 《Journal of General Plant Pathology》2007,73(1):15-21

Leaf blast suppression in multilines was evaluated based on the number of susceptible lesions observed in a pure stand of susceptible rice cultivar Sasanishiki, and in 1 : 1 and 1 : 3 mixtures of Sasanishiki and a resistant near-isogenic line, Sasanishiki BL4 or BL7, from 1998 to 2001. The number of lesions first observed in fields in the 1 : 1 and 1 : 3 mixtures were close to theoretical numbers calculated using the number of lesions observed in the pure stands and the ratios of the susceptible Sasanishiki in the mixtures. The ratio of the number of lesions in the 1 : 1 and 1 : 3 mixtures to the number in the pure stand was 0.29 and 0.09, respectively. The relationship between these ratios and the ratios of susceptible Sasanishiki in mixtures was defined in an equation to estimate the degree of leaf blast suppression. Validation studies for the ratios of the number of lesions in the 1 : 1 and 1 : 3 mixtures to the number in the pure stand were conducted in two different locations and showed that the ratios are almost acceptable. The calculated autoinfection to alloinfection ratio was 1.3 and 1.4 in the 1 : 1 and 1 : 3 mixtures, respectively, suggesting that the calculated ratio will affect the degree of leaf blast suppression. Thus, predictors were obtained to estimate leaf blast suppression for effective blast control in multilines.  相似文献   

Histopathological Characteristics of Endometrosis in Thoroughbred Mares in Japan: Results from 50 Necropsy Cases     

Michiko HANADA  Yousuke MAEDA  Masa-aki OIKAWA 《Journal of Equine Science》2014,25(2):45-52

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Identification of a novel cytotoxic protein, Cry45Aa, from Bacillus thuringiensis A1470 and its selective cytotoxic activity against various mammalian cell lines     

Okumura S  Saitoh H  Ishikawa T  Wasano N  Yamashita S  Kusumoto K  Akao T  Mizuki E  Ohba M  Inouye K 《Journal of agricultural and food chemistry》2005,53(16):6313-6318

Parasporal inclusion proteins produced by Bacillus thuringiensis strain A1470 exhibit strong cytotoxicity against human leukemic T cells when activated by protease treatment. One of the cytotoxic proteins was separated by anion exchange chromatography and gel filtration chromatography and designated Cry45Aa. Its gene was then expressed in recombinant Escherichia coli, in which the Cry45Aa precursor was accumulated in an inclusion body. It was solubilized in sodium carbonate buffer and processed with proteinase K, and cytotoxic activities of the protein against various mammalian cell lines were evaluated using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide assay. The protein exhibited high cytotoxic activity against CACO-2, Sawano, MOLT-4, TCS, and HL60 cells and moderate activity against U-937 DE-4, PC12, and HepG2 cells. On the other hand, the EC50 values against Jurkat, K562, HeLa, A549, Vero, COS-7, NIH3T3, CHO, and four normal tissue cells (human primary hepatocyte cells, UtSMC, MRC-5, and normal T cells) were >2 microg/mL.  相似文献   

Efficacy of ivermectin against live mites and eggs of Sarcoptes scabiei in pigs     

S Ohba  H Toriumi  M Takeishi  R Noda 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1989,51(5):981-985

Sows infested with Sarcoptes scabiei var. suis (ssvs) were treated with 75, 150 and 300 micrograms/kg of ivermectin by a single subcutaneous injection at the neck region. Compared to the numbers of mites and eggs just before injection, those on post treatment weeks (PTW) 1, 2 and 4 showed significant decreases. Especially at 300 micrograms/kg, the counts showed almost all mites and eggs were eradicated on PTW 1, manifesting ivermectin to possess potential effect on ssve without apparent abnormal side effect. Potential mitocide effect of ivermectin on ssvs was revealed.  相似文献   

Risk evaluation and tree improvement as components of intensive forest management in Japan     

Kihachiro Ohba 《Forest Ecology and Management》1983,6(3):245-262

Forestry practice differs from agricultural crops practice owing to the huge size of trees, their longevity and the variety of uncontrollable conditions under which they grow. Even with intensive forest management, the actual effects of investment of labour and materials in the forest seem to be fairly small because of the size of the forest and the long harvest rotation. Since we expect to produce both a higher quantity and a higher quality of timber with intensive forestry, it is very important to have adequate counter-measures to the fatal or destructive factors which affect forest establishment and growth. In Japan, in the field of forest tree breeding, we are making maps as information sources for decision making, in which iso-lines show the degrees of damage due to fatal factors such as pests and diseases. If there is more than one fatal factor overlapping in an area, it might be difficult to grow forests for commercial purposes. We have to decide how to use the forest land in these areas, or how to take counter-measures such as tending, chemical control, genetic improvement or even a change in the tree species. As forestry has a long harvest rotation, it is necessary to have ‘a quality control system’ in management, in which a follow-up assessment of planting materials, tending techniques in combination with site conditions, and fatal factors will be made. In this situation, forest tree improvement (breeding) seems to be of prime importance in intensive forest management in the future.  相似文献   

Loop-mediated isothermal amplification for the rapid, sensitive, and specific detection of the O9 group of Salmonella in chickens     

Okamura M  Ohba Y  Kikuchi S  Suzuki A  Tachizaki H  Takehara K  Ikedo M  Kojima T  Nakamura M 《Veterinary microbiology》2008,132(1-2):197-204

In the present study, the loop-mediated isothermal amplification (LAMP) assay was developed to amplify the fragments of the O9 Salmonella-specific insertion element and evaluated in the laboratory for its potential use in a field situation, such as poultry farms. Among the bacteria tested, a positive reaction was observed only for 128 strains of 6 serovars of the O9 group Salmonella, such as Enteritidis (SE) and Pullorum. The detection limit of the LAMP assay was 10(3)CFU/ml, which was more sensitive than that of the polymerase chain reaction (PCR) assay with the same target gene (10(6)CFU/ml). The final results were obtained within 30 min for the LAMP assay, while the PCR assay needed a total of 120 min. When the LAMP assay was applied to the enrichment broth mixed with cecal dropping samples either spiked with SE in vitro or excreted by SE-inoculated hens, the results were comparable to those of the conventional plating method including 2 separate enrichments. In conclusion, the LAMP assay developed in the present study is an effective method for the specific detection of the O9 group Salmonella serovars, including SE.  相似文献   

Effect of <Emphasis Type="Italic">Lactococcus lactis</Emphasis> K-C2 on the growth performance,amino acid content and gut microflora of amberjack <Emphasis Type="Italic">Seriola dumerili</Emphasis>     

Nguyen Thi Hue Linh  Setsuko Nagai  Noriko Nagasaka  Seika Okane  Yousuke Taoka 《Fisheries Science》2018,84(6):1051-1062

This study aimed to evaluate the effect of Lactococcus lactis K-C2 on the growth performance, microbial diversity, and release of free amino acids in the intestinal tract and the edible parts of young amberjack, Seriola dumerili. Fish were fed a diet with or without strain K-C2 (2?×?10¹⁰ cfu/g feed) for 25 days. The results indicated that the growth performance of fish in the treated group was significantly higher than those in the control group (p?<?0.05). The amount of five amino acids (aspartate, sarcosine, taurine, alanine, and arginine) in the gut content and 13 of 21 amino acids in the edible parts of fish in the treated group were significantly higher (p?<?0.05) than those in the control group. Sphingomonas, Propionibacterium, and Mycobacterium were observed in gut microflora of fish in both the control and treated groups. Staphylococcus and Kocuria were detected in one sample from the control and treated groups; Acinetobacter and Acidobacteria were found in one sample from the control group. L. lactis was only found in one sample in the treated group. In conclusion, the dietary administration of probiotic L. lactis stimulated growth, reduced feed consumption, and improved the nutritional value of cultured amberjack.  相似文献   

Reduced glutathione decreases energy expenditure in chicks exposed to separation stress     

Haruka YAMANE  Shozo TOMONAGA  Yousuke TSUNEYOSHI  D. Michael DENBOW  Mitsuhiro FURUSE 《Animal Science Journal》2009,80(3):291-295

Recently, we reported that intracerebroventricular (i.c.v.) injection of reduced glutathione (GSH) induces hypnotic and sedative effects under an acute stressful condition in chicks. However, no information is available on the effects of GSH on energy expenditure (EE) under stressful conditions. The purpose of the present study was to clarify whether i.c.v. injection of GSH affects EE of neonatal chicks, and whether EE is correlated with behavioral changes after isolation-induced stress. The EE was rapidly decreased by i.c.v. injection of GSH, but was increased 27 min after injection. This change in EE was correlated with behavioral changes in which GSH induced hypnotic and sedative effects shortly after injection, followed by a period in which activity increased. The present study demonstrates that central GSH initially causes lowered EE through hypnotic and sedative effects under an acute stressful condition in chicks.  相似文献   

Identification and characterization of <Emphasis Type="Italic">Choanephora</Emphasis> spp. causing Choanephora flower rot on <Emphasis Type="Italic">Hibiscus syriacus</Emphasis>     

Ji-Hyun?Park  Sung-Eun?Cho  Mai?Suyama  Yousuke?Degawa  Hyeon-Dong?ShinEmail author 《European journal of plant pathology / European Foundation for Plant Pathology》2016,146(4):949-961

Hibiscus syriacus, as a national flower of Korea, is most popularly used for ornamental purposes and includes numerous cultivars, and it is widely planted in temperate zones that feature hot summers. We investigated Choanephora flower rot on H. syriacus from 2012 to 2014 in Korea and Japan and confirmed Choanephora infection in several localities in both countries. Here, our objectives were to identify the main causal agent of Choanephora flower rot on H. syriacus and describe its morphological and molecular characteristics. We identified 44 out of 50 isolates as Choanephora cucurbitarum and the remainder as C. infundibulifera based on morphological characterization and phylogenetic analysis. The sequences of the internal transcribed spacer region (ITS) of ribosomal DNA and the D1/D2 region of the large subunit (LSU) rDNA of examined isolates were compared with sequences obtained from GenBank, and the analysis of the results revealed 100 % identity with the corresponding sequences of C. cucurbitarum and C. infundibulifera strains. Classification of the Choanephora species performed here according to the key described by Kirk (1984) corresponded with the results of the phylogenetic analysis of this study. Through intraspecific and interspecific mating tests, the characteristics of zygospore were described in details. Pathogenicity tests using both species showed the same symptoms, causing blossom blight and soft rot on the flowers, which were identical to those observed in the field. All identified causal agents of Choanephora rot were indeed Choanephora species, where C. cucurbitarum was identified in the majority, while the others were in the minority of examined samples.  相似文献