Construction of a cDNA library of Bemisia tabaci for use in the ''yeast two-hybrid screen'' method     

S. Ohnesorge  E. R. Bejarano 《EPPO Bulletin》2002,32(1):48-48

Bemisia tabaci was reported for the first time in the Mediterranean part of Croatia in 2000. It was found in glasshouses in the agricultural area between the towns of Trogir and Omis, on the following crops: Euphorbia pulcherrima , Thunbergia grandiflora , Cucumis sativus (cucumber), Solanum melongena (aubergine), Phaseolus spp. (beans), Ficus carica (fig), Rubus spp. and several weeds of the families Asteraceae and Solanaceae . In 2001, monitoring for the pest was organized all over the country, in each of the 21 counties. In each county, there were several monitoring points so that all the major vegetable and flower producers were included. A special effort was made to record the spread of B. tabaci in the region where it was first found, bearing in mind that optimal conditions for outdoor spread exist along the Adriatic coast. Yellow sticky traps and visual inspection are used to monitor for B. tabaci . Eradication measures are being applied, and regulatory measures have been taken to prevent further spread of B. tabaci to continental parts of Croatia.  相似文献   

Suitability of Amaranthaceae and Polygonaceae species as food source for the sugar beet weevil Asproparthenis punctiventris Germar     

Dittmann  Lena  Spangl  Bernhard  Koschier  Elisabeth H. 《植物病害和植物保护杂志》2023,130(1):67-75

Journal of Plant Diseases and Protection - This study explores the food plant spectrum of the sugar beet weevil (Asproparthenis punctiventris Germar; Coleoptera: Curculionidae), one of the most...  相似文献   

Application of urease inhibitor improves protein composition and bread‐baking quality of urea fertilized winter wheat     

Azin Rekowski  Monika A. Wimmer  Bernd Hitzmann  Bernhard Hermannseder  Heike Hahn  Christian Zrb 《植物养料与土壤学杂志》2020,183(2):260-270

Background: Nitrogen losses is an economic problem for wheat production and a high risk to the environment. Therefore, improved N fertilizer management is a key to increasing the N efficiency and minimizing N losses. To increase N efficiency, enhanced fertilizers such as urea combined with urease inhibitor can be used. Aims: The aim of present study was to evaluate the effects of different N forms on grain storage protein subunits in winter wheat and to examine whether the observed changes correlate with parameters of baking quality. Methods: The investigation was performed over two consecutive years at two locations in Germany. Protein subunits were analyzed by SDS‐PAGE. Results: Protein concentrations were similarly increased after fertilization with ammonium nitrate and urea + urease inhibitor. Analysis of the individual storage protein fractions indicated that both fertilizers specifically enhanced ω‐gliadins and HMW glutenins, but the effect was more pronounced in the ammonium nitrate treatment. Application of urea + urease inhibitor had greater influence on the protein composition and resulted in higher specific baking volume as well as the best fresh keeping ability, in comparison with urea treatment. Conclusion: Considering that the urea + urease inhibitor treatment resulted in almost comparable improvements of NUE and baking quality, with the additional benefit of reduced N losses in combination with easy handling, urea + urease inhibitor can be recommended as a viable alternative to both urea alone and ammonium nitrate treatments. This opens up an opportunity for the reduction of N loss in wheat production when use of urea is preferred.  相似文献   

Relation between soil organic matter and yield levels of nonlegume crops in organic and conventional farming systems   总被引：2，自引：0，他引：2  

Christopher Brock  Andreas Fließbach  Hans‐Rudolf Oberholzer  Franz Schulz  Klaus Wiesinger  Frank Reinicke  Wernfried Koch  Bernhard Pallutt  Bärbel Dittman  Jörg Zimmer  Kurt‐Jürgen Hülsbergen  Günter Leithold 《植物养料与土壤学杂志》2011,174(4):568-575

The aim of this study was to evaluate the interaction between yield levels of nonleguminous crops and soil organic matter (SOM) under the specific conditions of organic and conventional farming, respectively, and to identify implications for SOM management in arable farming considering the farming system (organic vs. conventional). For that purpose, correlations between yield levels of nonlegume crops and actual SOM level (C_org, N_t, C_hwe, N_hwe) as well as SOM‐level development were examined including primary data from selected treatments of seven long‐term field experiments in Germany and Switzerland. Yield levels of nonlegume crops were positively correlated with SOM levels, but the correlation was significant only under conditions of organic farming, and not with conventional farming treatments. While absolute SOM levels had a positive impact on yield levels of nonlegumes, the yield levels of nonlegumes and SOM‐level development over time correlated negatively. Due to an increased demand of N from SOM mineralization, higher yield levels of nonlegumes obviously indicate an increased demand for OM supply to maintain SOM levels. Since this observation is highly significant for farming without mineral‐N fertilization but not for farming with such fertilization, we conclude that the demand of SOM‐level maintenance or enhancement and thus adequate SOM management is highly relevant for crop production in organic farming both from an agronomical and ecological point of view. Under conventional management, the agronomic relevance of SOM with regard to nutrient supply is much lower than under organic management. However, it has to be considered that we excluded other possible benefits of SOM in our survey that may be highly relevant for conventional farming as well.  相似文献   

Hydrolysis of Adenosine Triphosphate by Trichloracetic Acid     

Bernhard A  Rosenbloom L 《Science (New York, N.Y.)》1949,110(2859):402-403

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Keratitis due to Histoplasma spp. in a horse     

Richter M  Hauser B  Kaps S  Spiess BM 《Veterinary ophthalmology》2003,6(2):99-103

A 5-year-old Holsteiner gelding from Germany was presented 2 months after a whitish discoloration of the left cornea was observed. Cytologic examination revealed intra- and extracellular globular structures, up to 4 micro m in size, consisting of a central spherical deeply basophilic body surrounded by an unstained halo. The structures were morphologically consistent with Histoplasma spp. Infection with Histoplasma organisms is not endemic in Europe. Topical use of fluconazole was successful in eliminating Histoplasma organisms within 10 days of initiation of treatment.  相似文献   

Serum concentrations of 1,25-dihydroxycholecalciferol and 25-hydroxycholecalciferol in clinically normal dogs and dogs with acute and chronic renal failure     

Gerber B  Hässig M  Reusch CE 《American journal of veterinary research》2003,64(9):1161-1166

OBJECTIVE: To compare serum concentrations of 1,25-dihydroxycholecalciferol (1,25-[OH]2D3) and 25-hydroxycholecalciferol (25-[OH]D3) in healthy control dogs and dogs with naturally occurring acute renal failure (ARF) and chronic renal failure (CRF). ANIMALS: 24 control dogs, 10 dogs with ARF, and 40 dogs with CRF. PROCEDURE: Serum concentrations of 1,25-(OH)2D3 were measured by use of a quantitative radioimmunoassay, and serum concentrations of 25-(OH)D3 were measured by use of a protein-binding assay. RESULTS: Mean +/- SD serum concentration of 1,25-(OH)2D3 was 153 +/- 50 pmol/L in control dogs, 75 +/- 25 pmol/L in dogs with ARF, and 93 +/- 67 pmol/L in dogs with CRF. The concentration of 1,25-(OH)2D3 did not differ significantly between dogs with ARF and those with CRF and was in the reference range in most dogs; however, the concentration was significantly lower in dogs with ARF or CRF, compared with the concentration in control dogs. Mean +/- SD concentration of 25-(OH)D3 was 267 +/- 97 nmol/L in control dogs, 130 +/- 82 nmol/L in dogs with ARF, and 84 +/- 60 nmol/L in dogs with CRF. The concentration of 25-(OH)D3 was significantly lower in dogs with ARF or CRF, compared with the concentration in control dogs. CONCLUSIONS AND CLINICAL RELEVANCE: The concentration of 1,25-(OH)2D3 was within the reference range in most dogs with renal failure. Increased serum concentrations of parathyroid hormone indicated a relative deficiency of 1,25-(OH)2D3. A decrease in the serum concentration of 25-(OH)D3 in dogs with CRF appeared to be attributable to reduced intake and increased urinary loss.  相似文献   

Classification of thermally modified wood by FT-NIR spectroscopy and SIMCA   总被引：1，自引：0，他引：1  

Helmut B?chle  Bernhard Zimmer  Gerd Wegener 《Wood Science and Technology》2012,46(6):1181-1192

Quality assessment of thermally modified wood has evolved as one of the major fields in the research on thermal modification of wood. This study investigates NIR spectroscopy in combination with the pattern recognition method of soft independent modeling of class analogies (SIMCA). Focus is put on identifying different treatment intensities of thermally modified samples of beech, ash, and Norway spruce. The results indicate that SIMCA classification based on NIR spectroscopy could be used for quality control of thermally modified wood. The method might be applicable for producers (pre-delivery checks) and customers (reception control). However, transfer from laboratory to industrial conditions needs further investigation.  相似文献   

Einfluß niedriger SO2-Konzentrationen auf die Aktivität einiger Glykosidasen der Assimilationsorgane verklonter Waldbäume     

Inga K. Bucher-Wallin  L. Bernhard  J.B. Bucher 《Forest Pathology》1979,9(1):6-15

Effect of low SO₂-concentrations on the activities of some glycosidases in the foliage of clonal forest trees. The aim was to find an early indicator of latent SO₂ injuries in a beech and a spruce clone. After exposure to 0.0, 0.05, 0.1 and 0.2 ppm for several weeks in outdoor fumigation chambers, the activities of β-glucosidase in the beech and β-galactosidase in the spruce clone and β-l,3-glucanase in both species were significantly raised. As the beech clone was very sensitive to SO₂, an indication of latent injury could be ascertained only in the 0.05 ppm treatment where the rise of the β-glucosidase activity appeared some days before necroses. Contrary to beech, the spruce clone was tolerant to all SO₂-treatments and the enzymatical changes could be used as early indicators of latent injuries.  相似文献   

Rundschau     

H. Pechhacker  B. Ohnesorge  F. Schimitschek 《Journal of pest science》1980,53(3):45-47

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