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1.
Mizuki OGAWA Yasutomo HORI Nobuyuki KANNO Naoki IWASA Takeshi TOYOFUKU Noriko ISAYAMA Akane YOSHIKAWA Ryota AKABANE Atsushi SAKATANI Hirosumi MIYAKAWA Huai-Hsun HSU Yuichi MIYAGAWA Naoyuki TAKEMURA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(4):705
Plasma N-terminal pro-atrial natriuretic peptide (NT-proANP) concentration increases with progression of myxomatous mitral valve disease (MMVD) in dogs. This multicentre, prospective study compared plasma NT-proANP, N-terminal pro-brain natriuretic peptide (NT-proBNP), ANP, and cardiac troponin I (cTnI) concentrations in dogs with MMVD for their characteristics and discriminatory ability to detect cardiac dilatation and congestive heart failure (CHF). Thirty-six healthy dogs and 69 dogs with MMVD were included. Clinical variables were obtained via physical examination, thoracic radiography, and echocardiography. The discriminatory ability of each cardiac biomarker (CB) to determine the presence or absence of cardiac dilatation (event 1) and CHF (event 2) was evaluated using the receiver operating characteristic curves. Plasma NT-proANP, NT-proBNP, and ANP concentrations showed a significant association with the left atrium/aorta ratio (P<0.01). The area under the curve of plasma NT-proANP and NT-proBNP concentrations were 0.72 and 0.75, respectively in event1 and 0.72 and 0.76, respectively in event2. Plasma NT-proANP and NT-proBNP concentrations showed sensitivity 80.0 and 80.0%; specificity 67.6 and 64.7% in event1 (cutoff value; 8,497.81 pg/ml and 1,453.00 pmol/l, respectively) and sensitivity 85.7 and 81.0%; specificity 60.4 and 64.6% in event2 (cutoff value; 8,684.33 pg/ml and 1,772.00 pmol/l, respectively). In dogs with MMVD, plasma NT-proANP, NT-proBNP, and ANP concentrations increase with left atrial enlargement. Particularly, plasma NT-proANP and NT-proBNP concentrations appeared to be equally useful in the discriminatory ability to detect cardiac dilatation and CHF. 相似文献
2.
1. The aim of this study was to examine the effects of dietary fibre sources on growth and on the development of the gastro-intestinal tract in growing geese. 2. Six-week-old female White Roman geese were divided at random into 6 groups with 6 dietary treatments. Diets were isoenergetic and isonitrogenous and contained alfalfa meal, barley bran, rice hulls, cellulose, pectin or lignin as the major dietary source of fibre. 3. Food intake was significantly higher in the barley bran group than in the other groups. Both daily weight gain and food conversion of the pectin and alfalfa meal groups were lower than those of the other groups. 4. In the 9-week-old geese, the relative weight and the length of the small intestine did not significantly differ among treatment groups. However, the relative weight was significantly lightest and the caecal length was significantly shortest ( P 0.05) in the pectin group. 5. Activities of amylase and cellulose hydrolases of the caecal contents were also highest in the pectin group. 6. From SEM micrographs, no morphological damage of the villi was observable in the different intestinal segments of the geese in any of the treatments. 相似文献
3.
Fong-Yuan LIN Yeu-Yang TSENG Kun-Wei CHAN Shu-Ting KUO Cheng-Hsiung YANG Chi-Young WANG Masaki TAKASU Wei-Li HSU Min-Liang WONG 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(9):1055-1062
Orf virus (ORFV), a member of parapoxvirus, is an enveloped virus with genome
of double-stranded DNA. ORFV causes contagious pustular dermatitis or contagious ecthyma
in sheep and goats worldwide. In general, detection of viral DNA and observing ORFV virion
in tissues of afflicted animals are two methods commonly used for diagnosis of orf
infection; however, isolation of the ORFV in cell culture using virus-containing tissue as
inoculum is known to be difficult. In this work, the ORFV (Hoping strain) isolated in
central Taiwan was successfully grown in cell culture. We further examined the biochemical
characteristic of our isolate, including viral genotyping, viral mRNA and protein
expression. By electron microscopy, one unique form of viral particle from ORFV infected
cellular lysate was demonstrated in the negative-stained field. Moreover, immunomodulating
and anti-influenza virus properties of this ORFV were investigated. ORFV stimulated human
monocytes (THP-1) secreting proinflammatory cytokines IL-8 and TNF-α. And, pre-treatment
of ORFV-infected cell medium prevents A549 cells from subsequent type A influenza virus
(IAV) infection. Similarly, mice infected with ORFV via both intramuscular and
subcutaneous routes at two days prior to IAV infection significantly decreased the
replication of IAV. In summary, the results of a current study indicated our Hoping strain
harbors the immune modulator property; with such a bio-adjuvanticity, we further proved
that pre-exposure of ORFV protects animals from subsequent IAV infection. 相似文献
4.
騰格里沙漠地区沙地土壤微生物学特性的研究 总被引:3,自引:0,他引:3
沙漠的严重危害主要是流沙的移动。因此,沙漠改造和治理中的重要工作之一。是采取各种措施固定流沙[1]。一般說来,固定流沙有两种方法:一种是植物固沙,一种是机械固沙。机械固沙是暂时的固沙法,为固定流沙不可缺少的一环;而植物固沙是一种最根本最可靠的永久固沙方法[2,3]流沙一经生长植物,它的基本性质就要改变,形成固定沙丘。这就为进一步改造利用沙漠,创造了先决条件。时的固沙法,为固定流沙不可缺少的一环;而植物固沙是一种最根本最可靠的永久固沙方法[2,3]流沙一经生长植物,它的基本性质就要改变,形成固定沙丘。这就为进一步改造利用沙漠,创造了先决条件。 相似文献
5.
W. H. HSU YAO-FANG RONG F. B. HEMBROUGH 《Journal of veterinary pharmacology and therapeutics》1989,12(3):283-288
The effects of jingsongling (JSL) and xylazine on heart rate (HR) and mean arterial pressure (MAP) were studied in five conscious male dogs. An i.v. injection of xylazine (1 mg/kg) caused a bradycardia, an initial hypertension, and a subsequent hypotension. An i.v. injection of JSL (1 mg/kg) caused a bradycardia and a 20-min hypertension without a subsequent hypotension. Atropine sulfate (45 micrograms/kg, i.v.) increased HR for 30 min without changing MAP, and antagonized JSL-induced bradycardia for at least 60 min. There was a subsequent rebound bradycardia. Atropine sulfate potentiated JSL-induced hypertension in both magnitude and duration. Yohimbine (0.1 mg/kg, i.v.), an alpha 2-adrenoceptor antagonist, increased HR and MAP for 110 and 70 min, respectively. Yohimbine not only failed to potentiate but even reversed the pressor effect of JSL in a dose-dependent manner. Yohimbine also caused a dose-dependent reversal of JSL-induced bradycardia. Tolazoline (5 mg/kg, i.v.), a nonselective alpha-adrenoceptor antagonist, increased MAP for 20 min without changing HR. Tolazoline also reversed JSL-induced hypertension and bradycardia. Prazosin (1 mg/kg), an alpha 1-adrenoceptor antagonist, decreased MAP and increased HR for at least 110 min. Prazosin reversed JSL-induced hypertension but failed to affect JSL-induced bradycardia. These results indicated that: (1) JSL-induced bradycardia and hypertension are mediated by alpha 2-adrenoceptors; (2) yohimbine and tolazoline may be useful in antagonizing these untoward reactions associated with JSL administration, whereas prazosin and atropine were not found to be beneficial in this regard. 相似文献
6.
比較大規模的传病試驗的結果証知小米红叶病病毒不借种子传播,很难或者不能用机械式方法传染。 相似文献
7.
过去,国內外許多学者証明,适当的应用微量元素和生长刺激剂处理种籽,对农作物苗期生长有促进作用,也常常收到提高产量的效果。关于应用于棉籽处理方面,波波甫应用了溴化鉀浸种,显著的提高了棉花的产量。 相似文献
8.
Franz ZEHETNER Narasimham L. VEMURI Chih-An HUH Shuh-Ji KAO Shih-Chieh HSU Jr-Chuan HUANG Zueng-Sang CHEN 《Soil Science and Plant Nutrition》2008,54(4):618-626
Feitsui reservoir, the major water supply of Taipei city, has shown declining water quality in recent years. The reservoir is located in an area famous for tea cultivation, and the question arises as to whether the hillside tea plantations have contributed to increased sediment and nutrient discharge into the reservoir. In this study, soil and phosphorus redistribution were examined along a steep tea plantation in the reservoir's catchment and the provenance of reservoir sediment was assessed. Fallout radionuclides were used as soil erosion/deposition tracers and as markers of sediment sources. Continuous fertilizer application has raised (inorganic) phosphorus levels in the studied tea plantation. The plantation's narrow bench terraces trap eroded material and slow down soil and nutrient translocation. Nevertheless, eroded soil and phosphorus have accumulated on a vegetated toeslope below the tea plantation. The reservoir sediment contained significant amounts of inorganic phosphorus and cesium-137, and application of a mixing model resulted in a surface soil contribution of approximately 30%. This points towards tea plantations as possible sediment and pollutant sources and underlines the necessity of soil conservation strategies, such as the maintenance of vegetated riparian buffer zones. However, other sources, such as landslide debris and urban wastewaters, cannot be entirely ruled out. Further research is needed to better characterize the catchment's soils and sediments and to improve sediment fingerprinting efforts. Potential point and non-point pollution sources need to be examined in detail to better understand how phosphorus enters the reservoir. 相似文献
9.
Hei-Ti HSU Peter P UENG Fang-Hua CHU Zhaohui YE Shyi-Don YEH 《Journal of General Plant Pathology》2000,66(2):167-175
A serologically and cytologically distinct gloxinia tospovirus (HT-1) previously isolated from a gloxinia plant infected with
Impatiens necrotic spot virus (INSV) when propagated in a high-temperature environment was characterized. Rabbit antisera produced for INSV and Tomato spotted wilt virus (TSWV) nucleocapsids (N) failed to react with HT-1 proteins in western blot analysis. The HT-1 antibodies reacted strongly
with homologous antigen but failed to react with INSV and TSWV. However, the HT-1 antiserum reacted in ELISA with Watermelon silver mottle virus (WSMV) from Taiwan and in western blot analysis with the WSMV N protein. A reciprocal test showed that the antiserum prepared
against the N protein of WSMV also reacted with the HT-1 N protein in both ELISA and western blot analysis. DNA probes derived
from the N gene of HT-1 or WSMV hybridized to RNAs prepared from plants infected with either virus. Stronger signals were
obtained with homologous than with heterologous reactions. Neither probe detected INSV or TSWV. The M and S RNAs of HT-1 were
sequenced. The M RNA contains two open reading frames (ORF) ; one in the sense orientation encoding a nonstructural (NSm)
protein of 308-amino-acids (aa) and the other in the ambisense orientation, a 1122-aa precursor of Gl and G2 glycoproteins.
The S RNA also contains two ORFs ; one in the sense orientation encoding a nonstructural (NSs) protein of 439 aa and the other
in the ambisense orientation, an N protein of 277 aa. HT-1 is distantly related to INSV and TSWV as shown by low nucleotide
(40–52%) and amino acid (28–48%) similarities in the four ORF sequences. The HT-1 virus shares high nucleotide (76–81%) and
amino acid (85–92%) similarities with WSMV and peanut bud necrosis virus (PBNV). Based on the serological properties and sequence
data, we propose that HT-1 is a distinct species of serogroup IV in the genus Tospovirus. This is the first time that a tospovirus similar to those found in the Far East and in Southeast Asia has been identified
in the US.
Received 16 October 1999/ Accepted in revised form 20 December 1999 相似文献
10.
The light‐vented bulbul Pycnonotus sinensis is a small resident passerine that is found widely in central and south China, North Vietnam, and some East Asian islands, including Hainan, Taiwan and the Ryukyu Islands. Its subspecies status has been under considerable debate. A total of 10 subspecies have been proposed, but only 4 are widely recognized, including 1 continental taxon, P. s. sinensis, and 3 insular subspecies: P. s. hainanus, P. s. formosae and P. s. orii. Two mitochondrial DNA genes and 9 microsatellite loci were used to investigate the genetic divergence of this species, to identify the evolutionary status of 2 insular subspecies (P. s. hainanus and P. s. formosae) and to uncover probable historical causes that shaped them. The results reveal that P. s. formosae has diverged significantly from the other 2 subspecies because of a substantial time of isolation for populations on Taiwan Island. There was no significant genetic differentiation between P. s. sinensis and P. s. hainanus. The notable morphological difference in P. s. hainanus might be attributed to a rapid, recent adaptation to the tropical environment of Hainan Island. The low genetic divergence between P. s. sinensis and P. s. hainanus might result from a recent divergence or gene flow between them. Two insular subspecies of light‐vented bulbul populations might have diverged recently from their continental relatives through a complex evolution history owing to island isolation, ecological isolation, and possibly even hybridization. Both P. s. hainanus and P. s. formosae should be considered important conservation units because of their morphological and genetic distinctiveness. 相似文献