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1.
李涛  刘磊  郑峥  杜永臣  李君明 《园艺学报》2015,42(6):1077-1084
目前已明确番茄抗晚疫病R基因表现明显的株龄相关抗性(Age-related Resistance,ARR),但抗晚疫病QTL的抗性规律尚不明确。以携带抗晚疫病基因Ph-3的栽培种番茄(Solanum lycopersicum)‘CLN2037B’和野生种醋栗番茄(S.pimpinellifolium)‘L3708’以及易感病材料栽培种番茄(S.lycopersicum)‘LA2818’为对照,对含有抗晚疫病QTL的多毛番茄(S.habrochaites)‘LA2099’、‘LA1033’和‘LA1777’是否也存在株龄相关抗性进行了分析。结果表明,携带抗晚疫病QTL的3个材料的6叶期和9叶期植株病情级数均比3叶期明显降低,表明QTL抗性与株龄相关。利用乙烯、水杨酸和茉莉酸素合成或缺失的突变体和病毒诱导基因沉默(Virus Induced Gene Silencing,VIGS)技术,初步明确了乙烯和水杨酸参与6叶期Ph-3基因介导的对晚疫病的抗性,而茉莉酸不参与。  相似文献   
2.
ABSTRACT

Partial replacement of synthetic chemical fertilizers by naturally occurring alternatives is environmentally recommended. Feldspars (F), quartz silica (S), and zeolites (Z) are silicon (Si)-rich minerals that may be utilized as Si fertilizers. This study aims to assess the agronomic efficiency (AE) of the mentioned minerals as Si fertilizers and to estimate Si-use efficiency (Si-UE) in sandy soil. A field experiment was carried out (summer seasons of 2016 and 2017) in which F, S, and Z were mixed with surface soil in an application rate 500 mg kg?1 soil with and without potassium humate (K-H, 2 mg kg?1 soil). Treatments were distributed in a complete randomized block design (CRBD) with three replicates including control before cultivation of soybean (Glycine max L.). Yield (kg ha?1) of soybean, available Si (mg kg?1) in soil and uptake (mg kg?1) of N, P, K, Cu, Fe, Mn, Zn, and Si by soybean seeds and straw were estimated. The most significant increase was by 67.87% followed by 38.69% was recorded for the S and S K-H treatments, compared to the control. Same treatments showed nonsignificant decrease in the available Si (mg kg?1) that may refer to partial replenishment of plant available Si (PAS) in soil and avoid significant deficiency. Silica treatments resulted in the most significant increase in the uptake (mg kg?1 soil) of Si, N, P, K, Cu, Fe, Mn, and Zn by seeds and almost by straw. Silica was more efficient agronomically than feldspar and zeolite. Absorption of more biocompatible Si-organo species may depend on Si source.  相似文献   
3.
This study assessed the toxicity of melamine against the unicellular eukaryotic system of Tetrahymena (T.) pyriformis exposed to 0, 0.05, 0.25, 0.5, 2.5, and 5 mg/mL of melamine. Cell growth curves of different cultures, the half maximum inhibition concentration (IC50) value of melamine, and morphological changes in cells were obtained via optical and transmission electron microscopic observation. The effects of eleven melamine concentrations, 0.25, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5 and 5 mg/mL, on protein expression levels of T. pyriformis were examined using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The results showed an obvious inhibitory effect of melamine on the growth of eukaryotic cells. Cell growth dynamics indicated that the IC50 value of melamine on T. pyriformis was 0.82 mg/mL. The cellular morphology was also affected in a concentration-dependent manner, with characteristics of atrophy or cell damage developing in the presence of melamine. The relative contents of the top four main proteins corresponding to peak mass-to-charge ratios (m/z) of 4466, m/z 6455, m/z 6514, and m/z 7772 in the MALDI-TOF-MS spectra were all found to be closely correlated with the melamine concentrations. In conclusion, exposure of eukaryotic cells to melamine could inhibit cell growth, cause changes in cytomorphology and even disturb the expression of proteins in a concentration-dependent manner. The described method of examining four sensitive proteins affected by melamine was also proposed to be used in a preliminary study to identify protein biomarkers in T. pyriformis.  相似文献   
4.
Abstract

The monograph examines the main ways to correct the productivity of agricultural crops, the component composition of soil organic matter, the morphological classification of humus, the agro-ecological and trophic functions of soil organic matter. Attention is paid to the environmental aspect of soil fertility and the fundamentals of the functioning of the soil—plant system. The effect of non-root treatment of plants with solutions of humic substances on the physiology and biochemistry of plants is described. The main directions of the effective solution of problems arising from adaptive intensification of the production process of agricultural crops in agro-landscape farming are substantiated. The monograph is intended for specialists in the field of agronomy, agro-chemistry, crop production, soil science, agro-ecology, as well as for graduate students and students of higher and secondary special educational institutions of these specializations.  相似文献   
5.
The effects of the interaction between tree species composition and altitude on soil microbial properties are poorly understood. In this study, soil samples (0–20?cm) were collected in August 2011 from Betula platyphylla and Picea crassifolia forests along two different altitudinal gradients. Soil microbial activity and biomass were measured using Biolog-ECO plates and phospholipid fatty acid analysis. Both of the forest soils were characterized by a significantly lower soil pH (p?p?相似文献   
6.
Tropical Animal Health and Production - Carcass performance of 12 fattened male buffalo calves equally grouped by slaughter weights of 300, 350, 400, and 450 kg was evaluated. Six...  相似文献   
7.
This study aimed to evaluate the effectiveness of hormonal treatments on ovarian activity and reproductive performance in Barki and Rahmani ewes during non‐breeding season. Forty‐eight multiparous ewes, 24 Barki and 24 Rahmani ewes were divided into two groups, 12 lactating and 12 dry ewes for each breed. Controlled internal drug release (CIDR) device was inserted in all ewes for 14 days in conjunction with intramuscular 500 IU equine chronic gonadotrophin (eCG) at day of CIDR removal. Data were analysed using PROC MIXED of SAS for repeated measures. Breed, physiological status and days were used as fixed effects and individual ewes as random effects. Barki ewes recorded higher (p < .05) total number of follicles, number of large follicles, serum estradiol concentration and estradiol: progesterone (E2:P4) ratio compared to Rahmani ewes. Lactating ewes recorded higher (p < .05) number of small follicles and lower concentration of total antioxidant capacity (TAC) compared to dry ewes. Number and diameter of large follicles recorded the highest (p < .05) values accompanied with disappearance of corpora lutea at day of mating. Serum progesterone concentration recorded lower (p < .05) value at day of mating and the highest (< .05) value at day 35 after mating. CIDR‐eCG protocol induced 100% oestrous behaviour in both breeds, but Rahmani ewes recorded longer (< .05) oestrous duration compared to Barki. Conception failure was higher (< .05) in Barki compared to Rahmani ewes. In conclusion, CIDR‐eCG protocol was more potent in improving ovarian activity in Barki compared to Rahmani ewes, but this protocol seems to induce hormonal imbalance in Barki ewes that resulted in increasing conception failure compared to Rahmani ewes.  相似文献   
8.
Journal of Crop Science and Biotechnology - Understanding genetic diversity of rice helps to improve its yield. Although many landraces of rice are grown in the coastal area of Bangladesh, their...  相似文献   
9.
ABSTRACT

This work aims to study the status of silicon (Si) as a plant nutrient in the soil of Bahtim long-term field experiment in Egypt under the effect of crop rotations system and continuous fertilization. The experiment has been set up since 1912 based on two main factors: (1) crop rotations: mono-cropping (MC), two-year rotation (2Y-R), and three-year rotation (3Y-R), and (2) Fertilization: mineral nitrogen N, phosphorus P, potassium K, and organic farmyard manure FYM. Available N, P, K, and Si in soil were estimated. Productivity of soil was evaluated using Berseem (Trifolium alexndrinum L.) cultivated and harvested in 2019. The plant-available Si (PAS) in soil decreased significantly compared to the control C in case of MC by 70.26%, 2Y-R by 85.09%, and 3Y-R by 92.65% in the direction of N > NP > NPK. Mineral fertilization decreased the PAS significantly by 12.84% N, 29.52% NP, 78.45% NPK compared to the control C in the order of MC > 2Y-R > 3Y-R. Berseem yield (t ha?1) increased significantly compared with the control C following the order C < N < NP < NPK. The most significant increase in the yield was recorded for the NPK treatments by 224.04%, and 200% in case of MC, 2Y-R, 3Y-R, respectively.  相似文献   
10.
Two field experiments were carried out in Egypt during two successive seasons (2007/2008 and 2008/2009). This study aimed to investigate the response of growth, yield quality and some metabolic constituents of onion (Allium cepa L. cv. ‘Giza 20’) to foliar application of putrescine (Put; 25, 50 and 100 mg L−1) and glutamine (Glut; 50, 100 and 200 mg L−1), the former a diamine and the latter an amino acid, either alone, or in combination. Foliar application of Put and Glut, either alone or in combination, significantly increased plant height, number of leaves, fresh weight of leaves/plant, fresh and dry weight/plant, leaf area, leaf area/plant, bulb length, bulb diameter and weight, as well as yield of onion and quality of bulbs. Total soluble sugars, sulfur compounds, total soluble phenols, total free amino acids and total photosynthetic pigment content in leaves were increased by increasing Put and/or Glut concentrations up to 100 and 200 mg L−1, respectively. Generally, foliar application of Put at 100 mg L−1 and Glut at 200 mg L−1 singly, or combined, effectively increased bulb yield and quality. In conclusion, the yield-contributing characters and quality of onion could be improved by application of Put and/or Glut.  相似文献   
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