Surgical implantation of temperature‐sensitive transmitters and data‐loggers to record body temperature in koalas (Phascolarctos cinereus)          下载免费PDF全文

D Adam  SD Johnston  L Beard  V Nicholson  A Lisle  J Gaughan  R Larkin  P Theilemann  A Mckinnon  W Ellis 《Australian veterinary journal》2016,94(1-2):42-47

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Plasma lipopolysaccharide elevations in cattle associated with early-stage infection by Fasciola hepatica     

D Marendy  L Gabor  SD George  A Parker  E Doyle 《Australian veterinary journal》2023,101(9):334-338

Fasciolosis is an endemic zoonotic parasitic disease with significant impacts on human health and both animal health and production. Early post-infection impacts on the host remain unclear. The objective of this study was to determine the changes, if any, to levels of endotoxin in cattle plasma in response to early-stage infection with Fasciola hepatica. Thirty-six (36) commercial bred cattle were experimentally infected with approximately 400 viable metacercariae. Plasma lipopolysaccharide (endotoxin) levels were examined on 24 occasions from 0 h before infection to 336 h after infection using the Limulus Amoebocyte Lysate chromogenic end point assay and compared with that of six (6) uninfected control animals. Peak lipopolysaccharide levels in infected animals were reached at 52 h after infection and returned to pre-infection levels at time 144 h after infection. Infected animals had significantly elevated lipopolysaccharide levels between 24 and 120 h after infection when compared to uninfected animals. The mean change in endotoxin units (EU)/mL over time after infection was statistically significant in infected animals. Elevations of lipopolysaccharide occurred in all infected animals suggesting a possible repeatable and titratable endotoxemia conducive to therapeutic agent model development.  相似文献   

Identification of oat-adapted isolates of cereal Septoria in the UK using a detached leaf technique     

H. W. JOHNSTON  P. R. SCOTT 《Plant pathology》1988,37(1):148-151

Isolates of Septoria spp. from cereals were tested for their relative pathogenicity to detached seedling leaves of three cereal species. Each of 23 isolates showed unequivocal adaptation to either oats, or wheat, or barley. Five oat-adapted isolates were from Prince Edward Island, Canada, where S. avenue is prevalent on oats; three were from the UK, where there are few oat crops and where adaptation to oats has not been reported.
Oat-adapted isolates tended to have pycnidiospores of above-average length and could be referred to S. avenae. Wheat-adapted and most barley-adapted isolates had shorter spores and could be referred to S. nodorum. UV-fluorescent pigment was produced on oxgall agar by all wheat-adapted isolates, but only by a minority of oat-adapted and barley-adapted isolates.
It is suggested that adaptation to cereal species is a more useful character than spore length for classifying isolates.  相似文献   

Bluetongue virus serotypes 1 and 3 infection in Poll Dorset sheep   总被引：1，自引：0，他引：1  

C. HAMBLIN  JS SALT  SD GRAHAM  K. HOPWOOD  AM WADE-EVANS 《Australian veterinary journal》1998,76(9):622-629

Objective To study the clinical signs following bluetongue virus serotypes 1 and 3 infection in Poll Dorset sheep.
Design A clinical and pathological study.
Procedure Twenty Poll Dorset sheep were inoculated with bluetongue virus serotypes 1 or 3, each inoculum having a different passage history. The sheep were examined daily and their clinical appearance and rectal temperatures recorded. Heparinised and non-heparinised blood samples were taken at intervals for virological and serological study. Gross pathological findings were recorded for several sheep at necropsy and tissue samples were collected from three sheep for virological studies.
Results All inoculated sheep developed clinical disease. The clinical signs and gross pathological changes varied considerably but were consistent with damage to the vascular endothelial system. There was a decline in the titres of infectious bluetongue virus and of antigen in tissues collected between 7 and 12 days after infection.
Conclusions The severity of disease was related to the speed of onset and duration of pyrexia and not the development or titre of viraemia. Generally, those animals with sensitive mouths, depression, coronitis, recumbency and reluctance to move were the most debilitated. Whole blood was the most reliable source of infectious virus from acutely and chronically infected and convalescent animals. However, tissue samples particularly spleen, collected from dead or killed animals suffering from either peracute or acute forms of disease were most appropriate for the rapid confirmation of a clinical diagnosis.  相似文献   

AN ENUMERATION OF THE MATURE OAK IN THE FOREST OF BERE     

JOHNSTON  D. R. 《Forestry》1956,29(2):154-168

The paper describes the methods used in the field and in theoffice to determine the standing volumes and increments of 100-and 130-year old oak in the Forest of Bere, Hampshire, duringworking-plan revision. The figures obtained show that the standingvolumes and increments of sessile oak (Quercus petraea Liebe)are considerably higher than those of pedunculate oak {Q. roburL.) on the three sites investigated, namely, clays, sands, andgravels, while for both species sands appear to be the mostfavourable and gravels the least favourable soil types. Therewas little difference in the mean values for increment and standingvolume between the 100- and the 130-year old pedunculate oak.  相似文献   

Effect of Oil Overlay on Inhibition Potential of Roscovitine in Sheep Cumulus‐Oocyte Complexes          下载免费PDF全文

LF Crocomo  WC Marques Filho  CMV Ulian  NS Branchini  DT Silva  CL Ackermann  FC Landim‐Alvarenga  SD Bicudo 《Reproduction in domestic animals》2015,50(3):410-416

Inhibitors of cyclin‐dependent kinases, as roscovitine, have been used to prevent the spontaneous resumption of meiosis in vitro and to improve the oocyte developmental competence. In this study, the interference of oil overlay on the reversible arrest capacity of roscovitine in sheep oocytes as well as its effects on cumulus expansion was evaluated. For this, cumulus‐oocyte complexes (COCs) were cultured for 20 h in TCM 199 with 10% foetal bovine serum (Control) containing 75 μm roscovitine (Rosco). Subsequently, they were in vitro matured (IVM) for further 18 h in inhibitor‐free medium with LH and FSH. The culture was performed in Petri dishes under mineral oil (+) or in 96 well plates without oil overlay (?) at 38.5°C and 5% CO₂. At 20 and 38 h, the cumulus expansion and nuclear maturation were evaluated under stereomicroscope and by Hoechst 33342 staining, respectively. No group presented cumulus expansion at 20 h. After additional culture with gonadotrophins, a significant rate of COCs from both Control groups (+/?) exhibited total expansion while in both Rosco groups (+/?) the partial expansion prevailed. Among the oocytes treated with roscovitine, 65.2% were kept at GV in the absence of oil overlay while 40.6% of them reached MII under oil cover (p < 0.05). This meiotic arrest was reversible, and proper meiosis progression also occurred in the Control groups (+/?). So, the culture system without oil overlay improved the meiotic inhibition promoted by roscovitine without affecting the cumulus expansion rate or the subsequent meiosis progression.  相似文献   

Controlled Cross Circulation in Dogs: Effects on Donor Hemodynamics     

CHARLES A. KUNTZ DVM  MS    SPENCER A. JOHNSTON VMD  Diplomate ACVS    JOHN JACOBSON DVM  MS  Diplomate ACVA    ROBERT A. MARTIN DVM  Diplomate ACVS  ABVP    MARTHA MOON DVM  Diplomate  ACVR    PETER SHIRES BVSc  Diplomate ACVS  JOHN C. LEE PhD 《Veterinary surgery : VS》1996,25(1):29-39

Controlled cross circulation (CCC) was performed in six pairs of dogs for 45 minutes with aortic cross clamping and cardioplegia. Data were collected in donor dogs at 10 minute intervals three times before, three times during, and three times after CCC and included arterial blood pressure, pulmonary capillary wedge pressure (PCWP), central venous pressure (CVP), cardiac index (CI), heart rate (HR), blood gas analysis, temperature, maximum rate of rise of left ventricular pressure dP/dt max/End diastolic volume (EDV), blood volume (BV), complete blood count (CBC) and activated clotting times (ACT). Pulse pressure (PP), systemic vascular resistance (SVR), oxygen delivery (Do₂), and left ventricular cardiac work (LVCW) were calculated. Arterial blood pressure, CVP, blood gas analysis, temperature, BV, CBC, and ACT were measured in recipient dogs. During CCC, donor hemodynamic changes resembled those observed in models of acute onset arteriovenous fistulas. Insidious BV shifts can occur despite the use of occlusive roller pumps. After CCC, donor hemodynamics resembled acute blood loss, characterized by decreases in mean arterial pressure (MAP), CVP, PCWP, and CI, and increases in SVR and dP/dt max/EDV. These changes were probably caused by pump imbalance and BV shift to the recipient dog.  相似文献   

EXERTIONAL RHABDOMYOLYSIS IN SHEEP     

R. L. PEET  J. DICKSON  H. MASTERS  J. JOHNSTON 《Australian veterinary journal》1980,56(3):155-156

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Feline urethrostomy—a critique and new method     

DUDLEY E. JOHNSTON 《The Journal of small animal practice》1974,15(7):421-435

A procedure for perineal urethrostomy is described in thirty cats. The opening for the stoma is separate from the main skin incision. Haemorrhage from the crura of the penis is controlled by ligation of the crura. Haemorrhage from the corpus cavernosum urethrae is controlled by a tourniquet around the penis, then by compression from the sutures which unite the urethra to the skin. The operation is applicable for primary procedures as well as to the repair of a stenosed perineal urethrostomy. Post-operative catheterization is avoided. The cats have been examined up to 30 months, median 14 months, post-operatively; one of the thirty urethrostomies stenosed at 7 months. Résumé. On décrit un procédé pour urétrostomie chez trente chats. L'ouverture des stoma est séparée de l'incision principale de la peau. On contrôle l'hémorragie de la cuisse du pénis par ligature de la cuisse. L'hémorragie du corps caverneux de l'urètre se contrôle au moyen d'un tourniquet autour du pénis, puis par compression des sutures qui unissent l'urètre à la peau. L'opération est applicable pour des procédés primaires de même que pour la remise en état d'une urétrostomie périnéale stenosée. Une cathétérisation postopératoire est évitée. On a examiné les chats jusqu'à 30 mois, médian 14 mois, de manière postopératoire. Une des trente urétro-stomies a sténosé à 7 mois. Zusammenfassung. Ein Verfahren für perineale Harnröhrenfistelung in 30 Katzen wurde beschrieben. Die Öffnung für das Stoma ist von dem Haupthauteinschnitt getrennt. Blutung von dem Crus penis wird durch Ligatur der Crura kontrolliert. Blutung von dem Corpus cavernosum urethrae wird durch eine Staubinde um den Penis unter Kontrolle gebracht, dann durch Kompression von den Suturen die den Urethra mit der Haut verbinden. Die Operation ist angebracht für primäre Verfahren sowie für Ausbesserung einer stenosierten perinealen Harnröhrenfistelung. Nachoperative Katheterisierung wird vermieden. Die Katzen bis zu 30 Monaten untersucht worden, durchschnittlich 14 Monate, postoperativlich; eine von den 30 Urethrostomien stenosierten um den 7. Monat.  相似文献   

Fungal disease in reptiles     

CJ Harvey  KD McSporran  RM Jakob-Hoff  JS Potter  SD Shaw 《New Zealand veterinary journal》2013,61(2)

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