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Sunil Kumar Patil Lakshminarayana Somashekar Sellappan Selvaraju Kolatalu V. Jamuna Shivashanmugam Parthipan Bala Krishnan Binsila Ramaswamy Venkatesha Prasad Janivara Parameshwaraiah Ravindra 《Reproduction in domestic animals》2020,55(8):998-1010
The region-specific expression of seminal proteins in testis and excurrent duct system determines the quality and function of the spermatozoa. In the present study, localization and expression of some of the seminal proteins such as insulin-like growth factor receptor 1β (IGF-1Rβ), phosphatidylethanolamine-binding protein 4 (PEBP4), α-tubulin and tissue factor pathway inhibitor 2 (TFPI2) were carried out in testis, excurrent duct system and spermatozoa of buffalo. IGF-1Rβ was localized in the cells of the seminiferous tubules of the testis, except in primary spermatocytes. The PEBP4 was localized only in the elongated spermatid, whereas α-tubulin and TFPI2 proteins were localized in all cells of the seminiferous tubule including spermatocyte. In the buffalo spermatozoa, IGF-1Rβ, PEBP4, α-tubulin and TFPI2 were localized in the acrosome region, the post-acrosomal region till the tail end, post-acrosome to the entire tail region and the equatorial region, respectively. The study indicates that IGF-1R, α-tubulin and PEBP4 proteins regulate spermatogenesis, whereas TFPI2 may be involved during the zona binding process of the buffalo spermatozoa. 相似文献
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V. Ganesan S. Ragupathy B. Parthipan D. B. Rajini Rani A. Mahadevan 《Biology and Fertility of Soils》1991,12(2):131-136
Summary We assessed vesicular-arbuscular mycorrhizal (VAM) fungi in coal, lignite, and calcite mine spoils. The level of VAM fungal infection and the population of VAM species in plants on the coal-waste sites were similar to those in plants on the calcite mine spoil. The plants on the coal-waste sites and their associated VAM fungi included Tephrosia purpurea (L.) Pers. with Glomus heterosporum and Sclerocystis microcarpus and Euphorbia hirta L. with G. botryoides and G. ambisporum. The recently revegetated lignite mine spoil supported Eragrostis sp. with G. globiferum, while the oldest revegetated lignite mine spoil supported Cocos nucifera L. with Scutellospora aurigloba and G. aggregatum, and Albizia lebbeck (L.) Benth. with Scutellospora dipapillosa and G. tenue. In the calcite mine spoil, roots of Mukia maderaspatana (L.) M. Romer with Sclerocystis sinuosa, G. dimorphicum, G. heterosporum and G. ambisporum and Datura metal L. with G. pustulatum and G. pulvinatum were found. The level of VAM infection and the population of VAM species in the oldest revegetated site (lignite mine spoil) were comparable with those in the coal-waste and calcite mine-spoil sites. 相似文献
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Summary Growth, ATP levels, and N2-fixing activity of Azotobacter chroococcum culture exposed to 500 g ml-1 of methyl isocyanate were investigated. In methyl isocyanate-exposed cells, the lag phase was extended up to 16 h but the control cells started to multiply immediately. ATP levels and N2-fixing activity as determined by acetylene reduction assay were markedly reduced in the treated cells. 相似文献
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