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1.
Cutler S Abdissa A Adamu H Tolosa T Gashaw A 《Comparative immunology, microbiology and infectious diseases》2012,35(1):17-21
Head and clothing lice from Jimma, Ethiopia were investigated for pathogenic bacteria. Genomic DNA from pools of lice was subjected to PCR analysis for Bartonella spp., Borrelia spp. Coxiella burnetii, Rickettsia spp. and Yersinia pestis. All 102 lice pools were negative for the afore mentioned pathogens, with the exception of Bartonella species found among 6 of 65 (9.2%) head lice pools and1 of 33 clothing lice pools. Identification was achieved by sequencing the ribosomal intragenic transcribed spacer region (ITS), revealing all to be Bartonella quintana. Although established as a clothing louse-borne infection, typically causing chronic bacteraemia, trench fever, bacillary angiomatosis and endocarditis, this has only been rarely reported among head lice. The higher numbers of infected head lice pools compared with clothing lice suggests their competence for maintaining this infection within Ethiopia. 相似文献
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The present study aimed to identify mycotoxins in edible tissues of Atlantic salmon (Salmo salar) using liquid chromatography coupled to hybrid quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS). After using a non-targeted screening approach and a home-made spectral library, 233 mycotoxins were analyzed. Moreover, the occurrence of mycotoxins in fish filets was evaluated, and their potential toxicity was predicted by in silico methods. According to the obtained results, forty mycotoxins were identified in analyzed salmon samples, the predominant mycotoxins being enniatins (also rugulosin and 17 ophiobolins), commonly found in cereals and their by-products. Thus, mycotoxin carry-over can occur from feed to organs and edible tissues of cultivated fish. Moreover, the toxicity of detected mycotoxins was predicted by the in silico webserver ProTox-II, highlighting that special attention must be paid to some less reported mycotoxins due to their toxic predicted properties. 相似文献
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Variation in Total and Extractable Elements with Distance from Roads in an Urban Watershed,Honolulu, Hawaii 总被引：2，自引：0，他引：2
Roads play a major role intransporting sediment associated nonpoint sourcepollutants to urban stream networks via storm drains. In urban areas the relationship of erodible soil toroads may be of critical importance in controllingmetal contributions to roads. Two 50-m transects(Park and School) were investigated perpendicular toroads in Manoa basin, Oahu, Hawaii. Concentrations ofnine elements were compared to background control soillocations and to five supplemental samples from nearbyrecreational parks. Sediment from curbside areas ofroads (road deposited sediment) was collected as thestarting point of each transect, and subsequently soilwas sampled from two depths (0–2.5 cm and 7.5–10.0 cm)along the transects. Total and 0.5 M HCl extractableconcentrations were determined for aluminum (Al),calcium (Ca), chromium (Cr), copper (Cu), iron (Fe),manganese (Mn), nickel (Ni), lead (Pb) and zinc (Zn)using either inductively coupled plasma-atomicemission spectroscopy (ICP-AES) or flame atomicemission spectroscopy (FAAS). Ca, Cu, Pb and Znexhibited anthropogenic enhancement, with Pb and Znhaving the greatest enrichment in road sedimentfollowed by locations nearest the road. Copperdisplayed a narrower band of contamination than eitherPb or Zn, and this may reflect larger aerosolassociations and more rapid fall velocities. Lead andZn exhibited substantial decay in concentration at 50 m compared to the road sediment, but enrichment wasstill apparent. The positioning of a band of soilbetween the road-curb area and the sidewalk for thePark transect facilitated deposition and storage oftrace metals, and with subsequent erosion by splash orconcentrated flow this area can account for continuedtransport of contaminated sediment to adjacent roadsurfaces. On the other hand the School transect hadno soil directly beside the road, and the nearestsample from the road (5 m) displayed enrichment butsubstantially lower than the Park transect. Thesepreliminary data suggest that remobilization of soilstored metals in close proximity to roads cansignificantly prolong the environmental contaminationof urban road systems and eventually stream sediments. 相似文献
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Belew S Hussien J Regassa F Belay K Tolosa T 《Tropical animal health and production》2012,44(7):1599-1603
An experimental study to investigate the susceptibility pattern of Haemonchus contortus to commonly used anthelmintics (albendazole, tetramisole, ivermectin, and triclobendazole) was conducted between June and September 2009. Adult H. contortus parasites were collected from a total of 30 sheep slaughtered at Jimma municipal abattoir. The anthelmintics were assessed for egg hatch inhibition ability against H. contortus eggs. The eggs (500 eggs/ml) were incubated with different concentrations (0.25, 0.125, 0.0625, 0.03125, and 0.015625?μg/ml) at 26°C for 48?h. Egg hatching inhibition of different anthelmintics at different concentrations was recorded. The overall mean percent inhibition showed that there was a significant difference (p?相似文献
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Gina Cannarozzi Solomon Chanyalew Kebebew Assefa Abate Bekele Regula Blösch Annett Weichert Dominik Klauser Sonia Plaza-Wüthrich Korinna Esfeld Moritz Jöst Abiel Rindisbacher Habte Jifar Victoria Johnson-Chadwick Ermias Abate Wuyan Wang Rizqah Kamies Negussu Husein Worku Kebede Kidist Tolosa Yazachew Genet Kidu Gebremeskel Brikti Ferede Firew Mekbib Federico Martinelli Hans Christian Pedersen Suhail Rafudeen Shimelis Hussein Muluneh Tamiru Naomi Nakayama Mike Robinson Ian Barker Samuel Zeeman Zerihun Tadele 《Euphytica》2018,214(2):31
Indigenous crops also known as orphan crops are key contributors to food security, which is becoming increasingly vulnerable with the current trend of population growth and climate change. They have the major advantage that they fit well into the general socio-economic and ecological context of developing world agriculture. However, most indigenous crops did not benefit from the Green Revolution, which dramatically increased the yield of major crops such as wheat and rice. Here, we describe the Tef Improvement Project, which employs both conventional- and molecular-breeding techniques to improve tef—an orphan crop important to the food security in the Horn of Africa, a region of the world with recurring devastating famines. We have established an efficient pipeline to bring improved tef lines from the laboratory to the farmers of Ethiopia. Of critical importance to the long-term success of this project is the cooperation among participants in Ethiopia and Switzerland, including donors, policy makers, research institutions, and farmers. Together, European and African scientists have developed a pipeline using breeding and genomic tools to improve the orphan crop tef and bring new cultivars to the farmers in Ethiopia. We highlight a new variety, Tesfa, developed in this pipeline and possessing a novel and desirable combination of traits. Tesfa’s recent approval for release illustrates the success of the project and marks a milestone as it is the first variety (of many in the pipeline) to be released. 相似文献
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Roba Yonas Tolosa Tadesse Daniel Assefa Zerihun Tesfaye Asamnew 《Tropical animal health and production》2020,52(6):3109-3112
Tropical Animal Health and Production - As per the report from the OIE in 2005, infectious laryngotracheitis (ILT) has not been yet reported in Ethiopia. Hence, considering the evident clinical... 相似文献
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A cross-sectional study of brucellosis in small ruminants was carried out from October 2008 to March 2009 in Jijiga District,
Somali Regional State of Ethiopia. Seven hundred thirty sera samples (421 of sheep and 309 of goats) were randomly collected
from purposively selected villages of the study area. Structured questionnaire format was developed, pre-tested and administered
to assess the perception of the community pertaining to brucellosis in sheep and goats. Sera samples were screened by Rose
Bengal Plate Test (RBPT), and all samples tested positive by the RBPT were subjected to Complement Fixation Test (CFT) for
confirmation. Of 12 serum samples that were positive by RBPT, 11 were positive by CFT. Statistically significant differences
were not observed between the species as well as the sex groups (P > 0.05); however, the variation between the age groups was statistically significant (P < 0.05). Analysis of the questionnaire survey suggests that improper handling of aborted materials, consumption of raw milk,
and lack of awareness about the disease, among others, might greatly contribute to further spread of brucellosis in their
livestock and exposes the community to a public health hazard. In general, the sero-prevalence in the study area was not so
high; nevertheless, appropriate brucellosis control and prevention methods should be implemented to circumvent future potential
for economic losses and the public health hazard of the disease. 相似文献
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Sero-prevalence and risk factors study of brucellosis in small ruminants in Southern Zone of Tigray Region, Northern Ethiopia 总被引：2，自引：2，他引：0
Teshale Teklue Tadele Tolosa Getachew Tuli Belay Beyene Birhanu Hailu 《Tropical animal health and production》2013,45(8):1809-1815
This study reports a prevalence and risk factor survey of brucellosis in small ruminants in Southern Zone of Tigray Region, Northern Ethiopia between October 2011 and April 2012 to determine the sero-prevalence of small-ruminant brucellosis and to identify associated risk factors for the occurrence of disease in small ruminants under extensive production system. Multistage random sampling was followed to select locations, flocks, and individual animals. Laboratory analysis of serum samples provided sero-prevalence estimates for flocks and geographic location. Information on risk factors at the individual and flock level was obtained by examination of individual animal and a questionnaire interview to flock owners. The overall individual animal-level sero-prevalence of brucellosis in small ruminants was 3.5 % and flock level sero-prevalence was 28.3 %, and the within-flock sero-prevalence was ranged from 0 % to 22.2 % based on the Complement Fixation Test. Multivariable logistic regression showed that the major risk factors for flock level sero-positivity were flock size and abortion history. This study showed that small-ruminant brucellosis is prevalent in the study area. Larger flock size and history of previous abortion in the flock were major risk factors identified for sero-positivity of small-ruminant brucellosis. 相似文献