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1.
L. Yildiz-Aktas S. Dagnon A. Gurel E. Gesheva & A. Edreva 《Journal of Agronomy and Crop Science》2009,195(4):247-253
Compounds with reactive oxygen species (ROS)-scavenging ability were studied. High-performance liquid chromatography (HPLC) pattern of polyphenols, contents of proline and carotenoids, and antiradical (AR) capacity were determined. The malonyldialdehyde (MDA) level was also assessed. Tolerant and sensitive cotton genotypes were compared, grown in the Aegean region of Turkey at normal (field capacity) and limited (1/3 field capacity) water supply. Chlorogenic acid isomers and flavonoids were identified in HPLC pattern of polyphenols. At normal water supply, the tolerant genotype was distinguished by a higher content of all polyphenol types, higher proline, carotenoids and AR capacity and lower MDA level compared with the sensitive genotype. In plants subjected to water deficit, a decline of all polyphenol compounds, carotenoids and AR capacity was observed. However, this response was less pronounced in the tolerant than in the sensitive genotype, i.e. despite the stress conditions imposed, the tolerant plants maintained a more effective defence system. The data are corroborated by the weaker structural membrane damage in the drought-exposed tolerant vs. sensitive genotype, according to the MDA test. Hence, diverse chemical types are involved in the non-enzymatic ROS-scavenging system of cotton plants and can be related to the drought tolerance of this important crop. 相似文献
2.
Turan N Yildirim F Altan E Sennazli G Gurel A Diallo I Yilmaz H 《Research in veterinary science》2012,93(3):1504-1507
The aim of the present study was to investigate abortion storms that occurred in the Marmara region of Turkey in 2008-2009 using a real-time PCR. Two aborted foetuses were necropsied and histo-pathological findings reported herein. Ten lungs, 3 brains and one nasal swab from 10 aborted foetuses, 6 nasal swabs and 3 vaginal swabs from aborting mares were included in this study. EHV-1 was isolated from the lung, liver and brain of 1 aborted foetus. EHV-1 DNA was detected in the lungs, livers and spleens of 2 necropsied foetuses and in 3 lungs from 10 foetuses submitted for diagnosis. A brain from one of the aborted foetuses was also positive for EHV-1 DNA. EHV-4 DNA was detected only in a nasal swab of one of the tested foetuses. Neither EHV-1 nor EHV-4 DNA was detected in the swabs of aborting mares. Sequence analysis of the glycoprotein B of the strains was performed and a phylogenetic tree was generated. The results indicated that 4 of the 5 Turkish EHV-1 strains (TR02, TR03, TR04 and TR05) clustered together; the fifth strain (TR01) was slightly removed from the group and clustered with other EHV-1 from various origins. Single nucleotide polyporphism (SNP in ORF30) associated with neuropathogenesis was not detected in any of the strains. At necropsy, sub-milier focal necrosis in the liver and spleen was observed. Microscopically, focal coagulation necrosis and marked eosinophilic intranuclear and intracytoplasmic inclusion bodies in the hepatocytes localised around the necrotic areas in the liver. Severe coagulation necrosis in white pulp of the spleen was also observed. 相似文献
3.
Huseyin Yilmaz Eda Altan Nuri Turan Aydin Gurel Damla Haktanir Kivilcim Sonmez Sezgin Deniz Ahmet Gulcubuk Emre Gur Gunes Sonmez Juergen A. Richt 《Journal of Equine Veterinary Science》2012
There has been an increase in outbreaks of neuropathogenic equine herpesvirus-1 (EHV-1) in the United States and Europe. However, the presence and frequency of neuropathogenic EHV-1 in Turkish horses are not known at present. This study aimed to investigate the frequency of EHV-1 and neuropathogenic strains of EHV-1 in the Marmara Region of Turkey. Samples were analyzed for the presence of EHV-1 and neuropathogenic EHV-1 by real-time PCR TaqMan probe assays. Overall detection rate of EHV-1 was 45.5% (51 of 112). The detection rates were 70.5% (24 of 34) in aborted fetuses, 53.3% (8 of 15) in neonatal deads, 66.6% (4 of 6) in foals, 40% (2 of 5) in dead mares, and 25% (13 of 52) in living mares. Overall detection rate of neuropathogenic EHV-1 was 7.8% (4 of 51), and the real-time PCR results were confirmed by sequencing. Neuropathogenic strains of EHV-1 were detected in the brain and lung of two mares with neurological disease but without a history of abortion, in the brain of a foal that died of respiratory disorder, and in the nasal swab from a mare with a history of abortion. On histopathology, nonpurulent meningoencephalitis, hemorrhages, and vasculitis were seen in the brain. In conclusion, results of this study indicated, for the first time, that the neuropathogenic EHV-1 is circulating in the Marmara Region of Turkey. The results of this study also show that the current risk for non-neuropathogenic strains is high, whereas risk for the neuropathogenic EHV-1-G2254 strain seems to be low. As outbreaks of EHV-1 continue in the Marmara region of Turkey, surveillance for neuropathogenic EHV-1 genotype should be maintained. 相似文献
4.
Combined use of hyperspectral VNIR reflectance spectroscopy and kriging to predict soil variables spatially 总被引：1，自引：0，他引：1
Hyperspectral visible near infrared reflectance spectroscopy (VNIRRS) and geostatistical methods are considered for precision
soil mapping. This study evaluated whether VNIR or geostatistics, or their combined use, could provide efficient approaches
for assessing the soil spatially and associated reductions in sample size using soil samples from a 32 ha area (800 × 400 m)
in northern Turkey. Soil variables considered were CaCO3, organic matter, clay, sand and silt contents, pH, electrical conductivity, cation exchange capacity (CEC) and exchangeable
cations (Ca, Mg, Na and K). Cross-validation was used to compare the two approaches using all grid data (n = 512), systematic selections of 13, 25 and 50% of the data and random selections of 13 and 25% for calibration; the remaining
data were used for validation. Partial least squares regression (PLSR) analysis was used for calibrating soil properties from
first derivative VNIR reflectance spectra (VNIRRS), whereas ordinary-, co- and regression-kriging were used for spatial prediction.
The VNIRRS-PLSR method provided better prediction results than ordinary kriging for soil organic matter, clay and sand contents,
(R
2 values of 0.56–0.73, 0.79–0.85, 0.65–0.79, respectively) and smaller root mean squared errors of prediction (values of 2.7–4.1,
37.4–43, 46.9–61, respectively). The EC, pH, Na, K and silt content were predicted poorly by both approaches because either
the variables showed little variation or the data were not spatially correlated. Overall, the prediction accuracy of VNIRRS-PLSR
was not affected by sample size as much as it was for ordinary kriging. Cokriging (COK) and regression kriging (RK) were applied
to a combination of values predicted by VNIR reflectance spectroscopy and measured in the laboratory to improve the accuracy
of prediction of the soil properties. The results showed that both COK and RK with VNIRRS estimates improved the predictions
of soil variables compared to VNIRRS and OK. The combined use of VNIRRS and multivariate geostatistics results in better spatial
prediction of soil properties and enables a reduction in sampling and laboratory analyses. 相似文献
5.
Volkan Gurel Kristyn Lambert Allen E. Page Alan T. Loynachan Katherine Huges John F. Timoney Michael Fettinger David W. Horohov John McMichael 《Veterinary research communications》2013,37(2):145-154
The addition of streptolysin-O (SLO) to the standard antibiotics regimen was shown to be superior to antibiotics alone after experimental infection of foals with Rhodoccocus equi (R. equi). The objective of this study is to investigate this response by determining the site-specific expression of extracellular matrix (ECM) and inflammatory response genes in biopsy samples taken from three distinct lung regions of the infected foals. Twenty-four foals were challenged by intrabronchial instillation of R. equi and assigned to four treatment groups: SLO/antibiotics adjunct therapy, antibiotics-only therapy (7.5 mg/kg clarithromycin and 5 mg/kg rifampin), SLO-only, and saline-only treatments. Treatments were administered twice daily for 16 days unless symptoms progressed to the point where the foals needed to be euthanized. Gene expressions were determined using custom-designed equine real-time qPCR arrays containing forty-eight genes from ECM remodeling and inflammation pathways. A non-parametric Wilcoxon signed-rank test for independent samples was applied to two pairs of time-matched comparison groups, SLO/antibiotics vs. antibiotics-only and SLO-only vs. saline-only, to document the significant differences in gene expressions within these groups. Several genes, MMP9, MMP2, TIMP2, COL1A1, COL12A1, ITGAL, ITGB1, FN1, CCL2, CCL3, CXCL9, TNFα, SMAD7, CD40, IL10, TGFB1, and TLR2, were significantly regulated compared to the unchallenged/untreated control foals. The results of this study demonstrate that enhancement of clinical responses by SLO is consistent with the changes in expression of critical genes in ECM remodeling and inflammatory response pathways. 相似文献
6.
Seventy isolates of Fusarium oxysporum (Schlechtend: Fr.) f. sp. melongenae Matuo and Ishigami (Fomg), the causal agent of eggplant Fusarium wilt, were tested for their interaction with different lines and cultivars to determine whether there was race-specific interaction. Also, a total of 13 cultivars were tested under greenhouse conditions to evaluate the presence of resistance to Fusarium wilt. The disease severity (%) and the area under disease progress curve (AUDPC) for each of the Fomg isolates were calculated by scale values. Results showed that neither of the resistant lines (LS1934 and LS2436) exhibited wilting symptoms, whereas susceptible local cultivars (cvs. ‘Kemer’ and ‘Hadrian’) displayed severe disease symptoms. There was no significant variation in the virulence, indicating the occurrence of a race or races among 70 Fomg isolates tested on resistant lines and susceptible cultivars. This may indicate a genetically homogeneous population structure of Fomg in Turkey. The eggplant cultivars Amadeo, Anatolia, Angela, Brigitte, Corsica, Hawk, Koksal, Nouma, Sharapova and Yula exhibited various degrees of susceptibility to three virulent Fomg isolates, but disease severity for AGR-703 was significantly different and lower among the tested eggplant cultivars, as well as rootstocks. Therefore, it is considered as the most appropriate for rootstock purposes in eggplant cultivations where Fusarium wilt is present in the soil. 相似文献
7.
Tuba Cigdem Oguzoglu Dilek Muz Volkan Yılmaz Feray Alkan Yılmaz Akça İbrahim Burgu 《Tropical animal health and production》2010,42(6):1175-1180
Five BVDV species 2 (BVDV-2) isolates were detected from cattle in Turkey. Phylogenetic analysis was performed based on the
5′ untranslated regions (UTRs) and E2 coding gene regions, respectively. The isolates were closely related to BVDV-2a strains
from North America and Canada used as references. This is the first report of the detection of BVDV-2 in naturally infected
Turkish cattle. It is important to consider BVDV-2 for planning future BVDV control and vaccination programs in Turkey. 相似文献
8.
Verma Sandeep Kumar Gantait Saikat Mukherjee Epsita Gurel Ekrem 《Journal of Crop Science and Biotechnology》2022,25(5):547-555
Journal of Crop Science and Biotechnology - Lycium barbarum (Solanaceae), commonly known as Goji (or wolfberry), is popular for its nutritive and medicinal properties and is called a “super... 相似文献
9.
Okatan Volkan Gündeşli Muhammet Ali Kafkas Nesibe Ebru Attar Şule Hilal Kahramanoğlu İbrahim Usanmaz Serhat Aşkın Mehmet Atilla 《Erwerbs-Obstbau》2022,64(2):247-260
The study was carried out to determine phenolic compounds, antioxidant activity, total lipids, saturated fatty acids, unsaturated fatty acids, and volatile organic compounds (VOCs) of 18 different walnut (Juglans regia L.) cultivars and genotypes grown in Usak province, Turkey. The biochemical compounds were identified for each cultivar and genotype and a comparative evaluation was carried out. The results showed that most of the biochemical compounds are significantly varying among each other (p?<?0.05). The existence and abundance of the biochemical compounds in the cultivars and genotypes have not only found to be a cause of differentiation, but they have been important parameters for the similarities among the cultivars and genotypes. Total phenolic content and antioxidant activity ranged from 750.67 to 1245.64?mg GAE ml?1 and from 42.46 to 56.50% in kernels, respectively. The highest contents of the phenolic compounds were noted from gallic acid (11991?mg kg?1), ellagic acid (1057.16?mg kg?1), catechin (425.4?mg kg?1), and rutin (216.6?mg kg?1), as an average of all cultivars and genotypes. The highest lipid contents were noted from ‘Franquette’ and ‘Fernette’ as 64.28% and 63.26%, respectively, while the lowest content was noted from ‘Oguzlar 77’ (52.52%). A total of 42 VOCs were described from the 18 different walnut cultivars and genotypes. Overall, results showed that the phenolic profiles, fatty acids composition, and VOCs play an important role in similarities and diversities among the cultivars. According to the results, 7 different clusters were developed from the 18 cultivars and genotypes. Herein, ‘Fernor’, ‘Maras 18’, ‘Arslan Local Type’ and ‘Cisco’ cultivars were found to have the lowest biochemical compositions. The superior cultivars or genotypes, in terms of the abundance of the biochemical composition, were found to be ‘Gulizar Hanim Type’, ‘Lara’, ‘Pedro’, ‘Tulane’, ‘Fernette’, ‘Balkan’ and ‘Franquetta’, where they were found to develop 3 clusters by 1:3:3, respectively.
相似文献10.