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Reza Ghanbarpour Nasrin Askari Masoud Ghorbanpour Yahya Tahamtan Khoobyar Mashayekhi Narjes Afsharipour Nasim Darijani 《Tropical animal health and production》2017,49(3):591-597
The aim of the present study was to determine the analysis of virulence genes and antimicrobial profile of diarrheagenic Escherichia coli isolated from diseased lambs. Two hundred ninety E. coli isolates were recovered from 300 rectal swabs of diarrheic lambs and were confirmed by biochemical tests. The pathotype determination was done according to the presence of genes including f5, f41, LTI, STI, bfp, ipaH, stx 1 , stx 2 , eae, ehlyA, cnf 1 , cnf 2 , cdIII, cdIV, and f17 by PCR method. Sixty-six isolates (23.72%) possessed the STI gene and categorized into entrotoxigenic E. coli (ETEC). Nine isolates (3.1%) and five isolates (1.72%) were positive for the cnf1 and cnf2 genes which categorized into necrotoxic E. coli (NTEC). Hundred and seventeen isolates (40.34%) harbored stx 1 and/or stx 2 and classified as Shiga toxin-producing E. coli (STEC). Thirteen isolates (4.48%) were assigned to atypical entropathogenic E. coli (aEPEC) and possessed eae gene. Two isolates (0.68%) were positive for ipaH gene and were assigned to entroinvasive E. coli (EIEC). Statistical analysis showed a specific association between eae gene and STEC pathotype (P?<?0.0001). The most prevalent resistance was observed against lincomycin (96.5%) and the lowest resistance was against kanamycine (56.02%), respectively. The high prevalence of STEC and ETEC indicates that diarrheic lambs represent an important reservoir for humans. ETEC may play an important role for frequent occurrence of diarrhea in lambs observed in this region. Due to high antibiotic resistance, appropriate control should be implemented in veterinary medicine to curb the development of novel resistant isolates. 相似文献
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Hossein Hamidinejat Masoud Ghorbanpour Leily Nabavi Mohammad Rahim Haji Hajikolaie Mohammad Hossein Razi Jalali 《Tropical animal health and production》2010,42(5):899-903
Toxoplasmosis, caused by Toxoplasma gondii, is a significant disease in livestock and humans. In Iran, studies shows that T. gondii infection in humans is relatively high and prevalence is associated mainly with consumption of undercooked meat or meat products.
We have examined 450 serum samples from female cattle distributed over all Ahvaz, the center of Khouzestan province, south-west
of Iran. IgG antibodies to T. gondii were assayed by the modified agglutination test using whole tachyzoites of T. gondii, and found in 71 (15.77%) of 450 cattle with titers of 1:25 in 38, 1:50 in 18, 1:100 in 11, 1:200 in three and 1:400 in one.
Titers of antibodies were decreased in cattle over 2 years old. These results indicate that T. gondii infection in cattle of Khouzestan is relatively considerable, but not very high and consumption of beef may be a source of
infection for humans in south-west of Iran. 相似文献
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Mashhadi AR Ghorbanpour M Kamali S Kohli RN 《Pakistan journal of biological sciences: PJBS》2010,13(22):1113-1115
In this study, the correlation between abomasal ulcers and presence of Clostridium perfringens (C. perfringens) was evaluated in 80 (50 affected and 30 non affected) randomly slaughtered buffaloes in Ahvaz slaughterhouse. Immediately after the slaughter, the abomasums was isolated and an incision was made on the wall of it. Then the abomasums were emptied and its interior was washed with water. The inner surface was examined for presence of abnormal lesion. Ulcers from affected and piece of abomasa from non affected buffaloes were cultured. Cultures were also made from contents of all samples and smears were also prepared from affected and non affected tissues. Cultures from content samples (12%) of 50 ulcerated abomasa were positive for C. perfringens while the agents were isolated from 1 content (3.3%) of non ulcerated abomasa. There was no statistical difference between presence of C. perfringens in contents and abomasal ulcers. Totally C. perfringens were isolated from ulcers of 6 (12%) ulcerated and tissues of 3 (10%) non ulcerated cases. Statistical analysis showed no correlation between presences of C. perfringens and abomasal ulcers. There was no statistical difference between sex and age of the affected animals. In conclusion C. perfringens seems not to be solely, a cause ofabomasal ulcers in buffaloes. 相似文献
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Mehdi Zarei Masoud Ghorbanpour Samaneh Tajbakhsh Nader Mosavari 《Tropical animal health and production》2017,49(6):1195-1200
Mycobacterium avium subsp. paratuberculosis (MAP) causes Johne’s disease, a chronic enteritis in cattle and other domestic and wild ruminants. The presence of MAP in tissues other than intestines and associated lymph nodes, such as meat and liver, is a potential public health concern. In the present study, the relationship between the results of rapid diagnostic tests of the Johne’s disease, such as serum ELISA, rectal scraping PCR, and acid-fast staining, and the presence of MAP in liver was evaluated. Blood, liver, and rectal scraping samples were collected from 200 slaughtered cattle with unknown Johne’s disease status. ELISA was performed to determine the MAP antibody activity in the serum. Acid-fast staining was performed on rectal scraping samples, and PCR was performed on rectal scraping and liver samples. PCR-positive liver samples were used for mycobacterial culture. Overall, the results of this study demonstrated that MAP can be detected and cultured from liver of slaughtered cattle and rapid diagnostic tests of Johne’s disease have limited value in detecting cattle with MAP infection in liver. These findings show that the presence of MAP in liver tissue may occur in cows with negative results for rapid diagnostic tests and vice versa. Hence, liver might represent another possible risk of human exposure to MAP. Given concerns about a potential zoonotic role for MAP, these results show the necessity to find new methods for detecting cattle with MAP disseminated infection. 相似文献
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Effect of Orally Administered Cisapride,Bethanechol, and Erythromycin on the Apparent Efficiency of Colostral IgG Absorption in Neonatal Holstein‐Friesian Calves 
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下载免费PDF全文 S.M. Ghoreishi M. Nouri A. Rasooli M. Ghorbanpour M.R. Mokhber‐Dezfouli P.D. Constable 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2015,29(2):714-720
Objective
To evaluate the effect of orally administered cisapride, bethanechol, and erythromycin on the absorption of colostral IgG in dairy calves.Animals
Twenty‐four healthy neonatal Holstein‐Friesian calves.Procedures
Calves were randomly assigned to one of the following treatments: 0.9% NaCl solution (2 mL, PO; negative control); erythromycin lactobionate (20 mg/kg BW, PO; anticipated to be a positive control); cisapride (0.5 mg/kg BW, PO); bethanechol chloride (0.5 mg/kg BW, PO). Calves were fed 3 L of pooled bovine colostrum containing acetaminophen (50 mg/kg) by suckling and oroesophageal intubation 30 minutes after each treatment was administered. Jugular venous blood samples were obtained periodically after the start of feeding and plasma total IgG, protein, acetaminophen, and glucose concentrations determined. Abomasal emptying rate was assessed by the time to maximal plasma acetaminophen concentration.Results
Oral administration of cisapride facilitated the absorption of colostral IgG and protein. The effect of cisapride on abomasal emptying rate could not be evaluated because cisapride appeared to interfere with acetaminophen metabolism. Based on the total IgG and total protein concentration‐time relationships, the beneficial effects of cisapride appeared to occur early after oral administration and were transient.Conclusions and Clinical Importance
Additional studies appear indicated to characterize the effect of cisapride dose on the magnitude and duration of its effect on facilitating the absorption of colostral IgG and protein. Identification of a nonantimicrobial method for increasing abomasal emptying rate, such as cisapride, will potentially provide a practical and effective method for facilitating transfer of passive immunity in colostrum‐fed dairy calves. 相似文献8.
Mahmood Sinaie Kazem Darvish Bastami Masoud Ghorbanpour Hossein Najafzadeh Majid Shekari Sara Haghparast 《Fish physiology and biochemistry》2010,36(4):1235-1242
It is of crucial importance to study on the biomarkers types to assess the specification of the pollutants and health status
of marine ecosystems in environmental evaluation projects. In this respect, total metallothionein biosynthesis and mercury
bioaccumulation in the liver and gills under acute mercury exposure were investigated in fish, Scat (Scatophagus argus). Spotted scat was exposed to different mercury concentrations (0, 10, 20, 30) for 24, 48, 72 h. Total MT levels were determined
by enzyme-linked immunosorbent assay (ELISA) method. Mercury contents were determined through cold vapor atomic absorption
spectrometry (CVAAS). Induction of MT during exposure was tissue specific, displaying different response pattern in gills
and liver. Mercury accumulated in liver much higher than in gills and the latter also showed lower MT level (P < 0.05). MT biosynthesis in liver showed a significant (P < 0.05) increase after exposure to different mercury concentration with increase in exposure time, whereas total MT content
did not significantly (P > 0.05) change in gills except for 72 h exposure at 30 μg l−1. Nonetheless, the relationship between MT biosynthesis and Mercury bioaccumulation in both tissues was significant (P < 0.05). The results suggest that this form of MT in S. argus was Hg inducible and could be extended as a biomarker of mercury pollution in marine ecosystems. 相似文献
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Gesunde Pflanzen - The current study explores the potential impact of seed priming with silicon nanoparticles (SiNPs, 0, 100, 200, 500?mg/L) and silicate (Si, 0, 1, 1.5, 2?mg/L) under... 相似文献
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