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1.
A 13-year-old neutered female Yorkshire terrier presented with a history of progressive episodic weakness and disorientation of 4 months duration. Physical and neurologic examinations were normal at presentation. Abdominal ultrasound revealed a mass involving the right adrenal gland. Standard planar scintigraphy was performed at 4, 18, and 24 hours after intravenous injection 185 MBq (5mCi) of 123I-labeled metaiodobenzylguanidine (123I-MIBG). An area of focal intense uptake was identified in the area of the right adrenal gland. A pheochromocytoma was confirmed histologically after surgical excision.  相似文献   
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The use of plant water status indicators such as midday stem water potential (Ψstem) and maximum daily trunk shrinkage (MDS) in irrigation scheduling requires the definition of a reference or threshold value, beyond which irrigation is necessary. These reference values are generally obtained by comparing the seasonal variation of plant water status with the environmental conditions under non-limiting soil water availability. In the present study an alternative approach is presented based on the plant’s response to water deficit. A drought experiment was carried out on two apple cultivars (Malus domestica Borkh. ‘Mutsu’ and ‘Cox Orange’) in which both indicators (Ψstem and MDS) were related to several plant physiological responses. Sap flow rates, maximum net photosynthesis rates and daily radial stem growth (DRSG) (derived from continuous stem diameter variation measurements) were considered in the assessment of the approach. Depending on the chosen plant response in relationship with Ψstem or MDS, the obtained reference values varied between −1.04 and −1.46 MPa for Ψstem and between 0.17 and 0.28 mm for MDS. In both cultivars, the approach based on maximum photosynthesis rates resulted in less negative Ψstem values and smaller MDS values, compared to the approaches with sap flow and daily radial stem growth. In the well-irrigated apple trees, day-to-day variations in midday Ψstem and MDS were related to the evaporative demand. These variations were more substantial for MDS than for midday Ψstem.  相似文献   
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Objective: To report a case of femoral artery thrombosis and its medical management in a young dog. Case summary: A 13‐week‐old, female Boxer puppy presented with hind limb paralysis after an abdominal crush injury. A left femoral artery thrombus was identified on ultrasound. No spinal trauma was visualized in imaging studies. Clinical management of arterial thrombosis in a 13‐week‐old puppy with streptokinase and dalteparin therapy is described. New or unique information provided: This paper describes an unusual presentation of arterial thrombosis. The medical management with streptokinase and dalteparin is also out of the ordinary. Images that document the development of compensatory circulation around the thrombosed vessel are included. Additionally, this paper also documents the altered development that occurred in this immature dog after the thrombotic event.  相似文献   
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The diagnostic test for Tritrichomonas foetus in bulls is microscopic examination of cultured preputial samples. Trichomonads other than T. foetus can be present in a preputial sample. Both a staining technique and a polymerase chain reaction assay were useful in differentiating between T. foetus and another trichomonad observed in samples from virgin bulls.  相似文献   
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The amount and type of dietary fiber influences the end-products of fermentation and thus fuel availability to intestinal tissue. Metabolic fuel usage was studied in intestinal cells isolated from dogs consuming a commercial diet or from rats consuming either a commercial rat diet or dog diet to examine preferential fuel usage, the effect of diet, and species differences. Production of 14CO2 was measured by incubating cells in media containing either D-[U-14C]glucose, [1-14C]n-butyrate, L-[U-14C]glutamine, or [1-14C]propionate with or without competing substrates. The presence of a mixture of 5 mM each of glucose, butyrate, propionate, and acetate and 1 mM glutamine in the media decreased CO2 production from glucose, glutamine, and propionate by canine enterocytes (P < 0.05) and from glutamine and propionate by canine colonocytes (P < 0.05). The presence of glutamine in the media decreased glucose oxidation by murine enterocytes, regardless of the diet. Similarly, glutamine decreased glucose oxidation by murine colonocytes (P < 0.05), but only when the rats had consumed the rat diet. Regardless of diet, murine colonocytes oxidized more butyrate (P < 0.01) than did enterocytes, and murine enterocytes tended (P < 0.07) to oxidize more glucose than did colonocytes. The proportion of propionate in colonic contents was higher in dogs than in rats (P < 0.02), and the proportion of butyrate tended to be higher in contents from rats than in those from dogs (P < 0.08). Colonic and cecal wet weights were decreased (P < 0.05) when rats were fed the commercial dog diet. Preferred utilization of substrates by isolated canine enterocytes and colonocytes differed from that of murine intestinal cells. These differences were only partially overcome by feeding the same diet to each species.  相似文献   
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Type I interferons (IFN) are important mediators of the host defense against viral infections in mammals. In humans multiple subtypes of IFN-alpha exist, most of which possess antiviral activity. Little is known about the type I IFN genes in cats and the role they may play in feline immunological responses to viruses. We have isolated cDNAs encoding five feline IFN-alpha (feIFN) subtypes that share from 95 to 99% amino acid sequence identity. FeIFN-alpha5 has five additional amino acids inserted at position 139, which are not present in the other four subtypes. Sequence identity of the feIFN proteins encoded by the five clones compared to human IFN-alpha2 is approximately 60%. Unlike most of the human subtypes, each of the five feline IFN sequences has an N-glycosylation recognition site. Expression of all five feIFN-alpha subtypes in Chinese hamster ovary (CHO) cells was confirmed by Western blot analysis, and all resulting proteins were glycosylated. The antiviral activity of each feIFN-alpha subtype produced in transiently transfected CHO cell cultures was tested in vitro. In addition, subtype feIFN-alpha6 was expressed in the yeast, Pichia pastoris. The resulting secreted mature recombinant protein was purified and demonstrated significant antiviral activity and induction of 2',5'-oligoadenylate synthetase activity in vitro.  相似文献   
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