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The results of a citation analysis of all publications by the Department of Phytopathology which appeared in the period 1969–1987, but based on citations recorded by the Science Citation Index only, are presented. Together with the results of an analysis of self-citations by the eight most cited authors they suggest, that phytopathology as a science is poorly covered by the Science Citation Index.Een citatie-analyse van alle in de periode 1969–1987 verschenen publikaties van de Vakgroep Fytopathologie omvatte uitsluitend citaties voorzover vermeld in de Science Citation Index (SCI).Enkele vermeldenswaardige resultaten betreffen de krommen, die de cumulatieve citatie-aantallen weergeven (Figuren 1b en 2b) en de grafiek, die de relatieve bijdrage van uiteenlopende publikaties aan het totaal der citaties uitbeeldt (Fig. 3). Uit Fig. 2b blijkt, dat het citatieverloop van een gemiddelde publikatie het karakter van een logistische groeikromme vertoont: na een lagfase en een fase van exponentiële groei vlakt de kromme at naar een stationaire fase. Uit Fig. 3 kan worden afgeleid, dat weinig artikelen veel, en vele weinig tot het totale aantal citaties bijdragen; zo nemen 25 van de in totaal 321 geciteerde artikelen 50% van het totale aantal (3659) citaties voor hun rekening!  相似文献   
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The cyclic hexadepsipeptide enniatin is known as a phytopathogenic compound from Fusaria causing necrosis and wilt. The molecule consists of three alternating residues each of a branched chain amino acid and D-hydroxyisovaleric acid (D-Hiv). Enniatins are synthesized by a 347kDa multienzyme (enniatin synthetase) via a thiol template mechanism. The corresponding gene esyn1 has an open reading frame of 9393 nucleotides and harbours two modules, one responsible for D-hydroxy acid activation and one for L-amino acid activation with an integrated N-methyltransferase domain. Such methyltransferases build an homologous group among N-methyl peptide synthetases. Enniatins are synthesized by step-wise condensation of dipeptidol building blocks in an iterative manner resembling fatty acid synthesis. A key enzyme in enniatin biosynthesis is the NADPH-dependent D-2-hydroxyisovalerate dehydrogenase, that supplies enniatin synthetase with D-Hiv. Enniatins contribute to the wilt toxic character of Fusaria. Virulence was significantly reduced in F. avenaceum after disruption of the esyn1 gene.  相似文献   
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Fusarium head blight (FHB) is an important disease of wheat, which can result in the contamination of grains with mycotoxins such as deoxynivalenol (DON). Artificial inoculation of flowering ears with conidial suspensions is widely used to study FHB diseases. Our goal was to compare four inoculation treatments in which a conidial suspension was sprayed on flowering ears and to study the effect of the application of moisture during kernel setting and filling with a mist-irrigation system. Ten wheat genotypes were inoculated with a DON-producing Fusarium culmorum strain. Inoculation treatments varied in time of application of the inoculum (morning or evening) and in the method of controlling humidity during inoculation (bagging or mist irrigation). A wet season was simulated with a mist-irrigation system, keeping the crop canopy wet for at least 26 days after flowering. The severity of FHB symptoms (area under disease progress curve (AUDPC)), yield loss and DON contamination in the grains were determined. AUDPC data obtained with the different inoculation treatments were highly correlated (r=0.85–0.95). Mist irrigation after inoculation resulted in a higher mean disease severity, but in a overall lower toxin contamination as compared to the non-irrigated treatments. Genotypic differences in DON accumulation were present: for one wheat line toxin contamination significantly increased when irrigated, while two genotypes accumulated significantly less toxin. The closest relationships (r=0.73–0.89) between the visual symptoms and the DON content were obtained under moderate mean infection pressure. This relation between visual symptoms and the DON content deteriorated at higher infection levels.  相似文献   
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OBJECTIVE: To evaluate a combined transcutaneous carbon dioxide pressure (tcPCO(2)) and pulse oximetry sensor in sheep and dogs. ANIMALS: 13 adult sheep and 11 adult dogs. PROCEDURES: During inhalation anesthesia, for the first 10 minutes following sensor placement, arterial blood gas was analyzed and tcPCO(2) was recorded every 2 minutes. Subsequently, the animals were hyper-, normo-, and hypoventilated. The simultaneously obtained tcPCO(2) and PaCO(2) values were analyzed by use of Bland-Altman statistical analysis. RESULTS: Mean +/- SD overall difference between tcPCO(2) and PaCO(2) 10 minutes after sensor application was 13.3 +/- 8.4 mm Hg in sheep and 8.9 +/- 12 mm Hg in dogs. During hyper-, normo-, and hypoventilation, mean difference (bias) and precision (limits of agreement [bias +/- 2 SD]) between tcPCO(2) and PaCO(2) values were 13.2 +/- 10.4 mm Hg (limits of agreement, -7.1 and 33.5 mm Hg) in sheep and 10.6 +/- 10.5 mm Hg (limits of agreement, -9.9 and 31.2 mm Hg) in dogs, respectively. Changes in PaCO(2) induced by different ventilation settings were detected by the tcPCO(2) sensor with a lag (response) time of 4.9 +/- 3.5 minutes for sheep and 6.2 +/- 3.6 minutes for dogs. CONCLUSIONS AND CLINICAL RELEVANCE: The tcPCO(2) sensor overestimated PaCO(2) in sheep and dogs and followed changes in PaCO(2) with a considerable lag time. The tcPCO(2) sensor might be useful for noninvasive monitoring of changes but cannot be used as a surrogate measure for PaCO(2).  相似文献   
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Summary Traditional meteorological estimates of evapotranspiration include empirical crop factors which are inadequate for scheduling high frequency irrigation. The performance of a transpiration model was tested and adapted to suit the operational requirements of automated irrigation systems. Hourly measurements of global solar radiation, air temperature, humidity and wind speed, obtained from an automatic weather station are inputs to the model. Additional inputs include daily updated data of plant height and leaf area index. This information is processed to determine the active coupling surface between the crop and the atmosphere. The model takes into account the resistance of the leaf to diffusion of water vapor.Calculated transpiration, based on the model, matched very closely measurements of latent heat flux in an irrigated cotton field. It was also in good agreement with water uptake measured in stems of the cotton plants using a heat pulse technique. The test also showed that implementation of the model in the field under study would have improved the efficiency of water application.Contribution from the Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel, No. 1855-E, 1986 series  相似文献   
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An incubation experiment was carried out to investigate whether salinity at high pH has negative effects on microbial substrate use, i.e. the mineralization of the amendment to CO2 and inorganic N and the incorporation of amendment C into microbial biomass C. In order to exploit natural differences in the 13C/12C ratio, substrate from two C4 plants, i.e. highly decomposed and N-rich sugarcane filter cake and less decomposed N-poor maize leaf straw, were added to two alkaline Pakistani soils differing in salinity, which had previously been cultivated with C3 plants. In soil 1, the additional CO2 evolution was equivalent to 65% of the added amount in the maize straw treatment and to 35% in the filter cake treatment. In the more saline soil 2, the respective figures were 56% and 32%. The maize straw amendment led to an identical immobilization of approximately 48 μg N g−1 soil over the 56-day incubation in both soils compared with the control soils. In the filter cake treatment, the amount of inorganic N immobilized was 8.5 μg N g−1 higher in soil 1 than in soil 2 compared with the control soils. In the control treatment, the content of microbial biomass C3-C in soil 1 was twice that in soil 2 throughout the incubation. This fraction declined by about 30% during the incubation in both soils. The two amendments replaced initially similar absolute amounts of the autochthonous microbial biomass C, i.e. 50% of the original microbial biomass C in soil 1 and almost 90% in soil 2. The highest contents of microbial biomass C4-C were equivalent to 7% (filter cake) and 11% (maize straw) of the added C. In soil 2, the corresponding values were 14% lower. Increasing salinity had no direct negative effects on microbial substrate use in the present two soils. Consequently, the differences in soil microbial biomass contents are most likely caused indirectly by salinity-induced reduction in plant growth rather than directly by negative effects of salinity on soil microorganisms.  相似文献   
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