利用PCR对鸭瘟和鸭"传染性肿头症"的鉴别诊断   总被引：4，自引：0，他引：4  

齐雪峰  杨晓燕  罗薇  岳华  刘内生 《四川畜牧兽医》2003,30(10):21-21

本文利用聚合链反应(PCR)技术对鸭瘟和鸭“传染性肿头症”进行鉴别诊断。结果证明该方法具有特异、快速的特点。可对这两种临床症状较为相似的鸭传染性疾病进行快速鉴别诊断。  相似文献   

国外松芽流脂病发生与防治     

翁永发  陈雁  汪国华  毛承芳  朱云有 《浙江林学院学报》2001,18(3):286-289

近年来,浙江省局部地区国外松纯林受芽流脂病危害较为严重.通过定点现察、分离培养、光镜检查、电镜现察、微量元素测定和用硼砂防治罹病松树等方法,基本查清国外松芽流脂病在浙江省的发生情况,明确了国外松芽流脂病的3种症状顶芽枯死型、丛枝型和枯梢型.国外松芽流脂病是由于松树自身和土壤中的微量元素--有效硼含量不足引起的生理性病害.用喷洒3.0g@kg-1或5.0g@kg-1硼砂溶液防治该病效果显著,防治区病情指数与对照相比平均下降38.5,松树的年平均高生长量也比对照大14.8cm.表4参4  相似文献   

家禽核酸疫苗的研究进展     

杨晓燕  罗薇  岳华  杨发龙 《四川畜牧兽医》2002,29(4):33-34

核酸疫苗是当今疫苗研究的最前沿领域，本文就核酸疫苗在家禽传染病中的应用做一综述。  相似文献   

60Co辐射诱变姬松茸突变株J3子实体不同部位的氨基酸分析研究     

黄挺俊  翁伯琦  肖淑霞  王义祥  江枝和 《食用菌学报》2005,12(4):13-16

测定并比较了60Co辐射诱变姬松茸突变株J3与原菌株J1子实体不同部位的氨基酸含量.结果表明,除菌柄和盖皮外,姬松茸突变株J3子实体其它部位的必需氨基酸、儿童氨基酸、硫氨酸、支链氨基酸、鲜味氨基酸、芳香族氨基酸和甜味氨基酸均高于原菌株J1.姬松茸突变株J3子实体中谷氨酸含量占氨基酸总量的比例分别比原菌株J1、α-酪蛋白和卵清蛋白提高34.88%、56.68%和122.32%.  相似文献   

Inhibitory effects of LDL-ACM complex on subcutaneous implanted tumors in nude mice()     

JIANG An-li  KANG Lu-dong  ZHAO Chun-hua  BI Wen-xiang  HU Xiao-yan  KONG Feng  XU Song-de 《园艺学报》2003,19(1):55-58

AIM: In order to evaluate the applicable value of LDL as a targeted vehicle for chemotherapeutic agents, we investigated and compared the inhibitory effects of LDL-ACM complex and free ACM on nude mice's subcutaneous implanted tumors derived from gastric cancer cell lines, SGC-7901 and NKM-45. METHODS: LDL-ACM complex was prepared and the tumor model of nude mice was established by subcutaneous implantation of SGC-7901 and NKM-45. Then, the groups of nude mice developed subcutaneous implanted tumors were received either LDL-ACM complex or free ACM. Subsequently, the tumor size, weight and leukemia cell counts were measured and the rates of tumor-inhibition and the survival were compared among the groups. RESULTS: The inhibitory effects of LDL-ACM complex on the tumors, especially on SGC-7901 implanted tumors were much more obvious than that of free ACM. It was also indicated that the action of LDL-ACM complex was mediated by LDL receptor. CONCLUSION: These results showed that LDL-ACM complex had significant inhibitory effects on the implanted tumors and the effect might be mediated by LDL receptor.  相似文献   

中国苜蓿繁育概述   总被引：8，自引：0，他引：8  

杨培志陈小燕  赵锁柱侯聪芬 《四川草原》2004,(2):35-38

回顾了建国以来国内苜蓿的育种发展状况,进行了苜蓿的种质评价与筛选,地方品种的系统化,育成了抗寒新品种草原1号、草原2号,抗病品种中兰一号,耐盐品种中苜一号,耐牧根蘖品种公农3号、中图3号、中图4号,高产的公农1号等。提出了在我国苜蓿育种方法上,杂交育种与基本育种方法在当代育种中将继续存在,生物技术在育种中的应用将加快育种进程;指出了我国苜蓿育种存在的问题是育种体系不健全,育种手段方法落后,抗性育种缓慢,对引入品种缺少理论与实践的鉴定、评价,国内育成品种少,高层知识力量薄弱等。  相似文献   

稻-鸭生态系统中二化螟消长动态研究   总被引：8，自引：1，他引：8  

刘小燕  杨治平  黄璜  胡立冬  刘大志  陈艳芬 《中国植保导刊》2004,24(12):9-12

2003年4～10月,在稻鸭共栖田中,对二化螟发生规律的系统研究结果表明,稻田养鸭能显著减轻二化螟的为害。与对照相比,中稻田养鸭区二化螟为害株率减少13.4%～47.1%;晚稻田减少62.2%。稻鸭共栖能减少化学农药的使用量,具有较好的经济、社会和生态效益。  相似文献   

Cortical gene expression pattern in rat focal cerebral ischemia     

ZHANG Zhan-jun  YING Kang  WANG Zhong  ZHANG Xiao-yan  LIU Jian-xun  HUANG Yan  XU Li  WEI Cui-e  WANG Yong-yan 《园艺学报》2004,20(8):1427-1433

AIM: To investigate the genes differential expression in cortex during rat focal cerebral ischemia.METHODS: cDNA microarray chips containing numerous cDNAs were used to investigate the gene expression pattern between samples of focal cerebral ischemia and sham-control operation rats. RESULTS: Two hundred and eleven genes differentially expressed were screened out, among these genes, up-and down-regulated genes were 199 and 12, respectively. CONCLUSIONS: The analysis of gene expression pattern of focal cerebral ischemia based on cDNA microarray can realize high-throughput screening of the genes associated with the focal cerebral ischemia. The differential expression of genes may be related to the pathogenesis of focal cerebral ischemic diseases.  相似文献   

Effects of Gensenosides Rb1 on proliferation of human acute promyelocytic leukemia (HL60) cells and expression of glucocorticoid receptor α     

LI Yong  ZHU Xiao-yan  LING Chang-quan 《园艺学报》2004,20(9):1572-1575

  相似文献   

冷季放牧补饲对甘肃高山细毛后备母羊瘤胃代谢参数及瘤胃微生物数量的影响   总被引：1，自引：0，他引：1  

靳继鹏  郭武君  张筱艳  张昌吉  张勇  王春辉  张利平 《草业学报》2018,27(7):93-103

旨在筛选出甘肃高山细毛羊后备母羊科学合理的补饲料,改变牧区由于营养供给不足导致甘肃高山细毛羊2.5岁才能配种的饲养方式。试验选择甘肃高山细毛羊(12月龄)48只,随机分为试验a(1号料)、b(2号料)、c(3号料)组和对照组(不补饲),进行90 d放牧补饲试验。结果表明,1)瘤胃pH试验a组和b组差异极显著(P<0.01)、c组显著(P<0.05)低于对照组,瘤胃氨态氮、蛋白氮、总氮浓度试验组均极显著高于对照组(P<0.01),总挥发性脂肪酸(VFA)试验a组和b组均极显著高于对照组(P<0.01),b组显著高于c组(P<0.05);乙酸和丙酸摩尔比a组极显著高于对照组(P<0.01),b和c组显著高于对照组(P<0.05);丁酸摩尔比a组极显著高于c组(P<0.01);乙/丙b组显著低于对照组(P<0.05)。2)瘤胃纤维降解菌和原虫数目试验组均极显著(P<0.01)或显著(P<0.05)高于对照组。综上所述,补饲均能正向影响甘肃高山细毛羊后备母羊瘤胃代谢参数及瘤胃微生物数量,以1号料补饲效果最优,2号料也能起到理想效果。  相似文献