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The effects of root colonization by the arbuscular mycorrhizal (AM) fungus Glomus intraradices Schenck and Smith on growth, flower and fruit production, and fruit quality were studied in field-grown tomato plants exposed to varying intensities of drought stress. Inoculated (M+) and non-inoculated (M−) tomato seedlings were exposed to varying intensities of drought stress by adjusting irrigation intervals. Mycorrhizal plants had significantly higher uptake of N and P in both roots and shoots regardless of intensities of drought stress. AM inoculation also significantly increased shoot dry matter and the number of flowers and fruits. The fruit yields of M+ plants under severe, moderate, mild drought-stressed conditions were higher than M− plants by 24.7%, 23.1%, 16.2% and 12.3%, respectively. Furthermore, M+ plants produced tomato fruits that contain significantly higher quantities of ascorbic acid and total soluble solids (TSS) than M− plants. Mycorrhizal effects increased with increasing intensity of drought. The overall results suggest that mycorrhizal colonization affects host plant nutritional status, water stratus and growth under field conditions and thereby alters reproductive behaviour, fruit production and quality of fruits under both well-watered and drought-stressed conditions.  相似文献   
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The impact of drought conditioning on the ability of eight-week-old jack pine (Pinus banksiana Lamb.) seedlings to withstand drought was assessed. Two progressive cycles of drought conditioning significantly increased the survival of seedlings subjected to a subsequent prolonged drought. The in vivo accumulation of several root membrane proteins during drought conditioning was correlated with an increase in seedling survival. A group of root proteins, ranging in molecular mass from 43 to 47 kDa, increased accumulation during one cycle of drought conditioning and to a lesser extent during two cycles of drought conditioning. The accumulation of several low molecular mass membrane and soluble proteins also increased during drought conditioning, suggesting that these proteins may play an important role in the enhancement of drought tolerance. In vitro translation studies showed a general increase in the abundance of protein products encoded by mRNAs from drought-conditioned seedlings. Although the majority of the in vitro translation products appeared in both control and drought-conditioned seedlings, one mRNA encoding a 15 kDA translated protein was more prominent during the second cycle of drought conditioning.  相似文献   
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Pressure-assisted thermal processing (PATP) is being widely investigated for processing low acid foods. However, its microbial safety has not been well established and the mechanism of inactivation of pathogens and spores is not well understood. Fourier transform infrared (FT-IR) spectroscopy was used to study some of the biochemical changes in bacterial spores occurring during PATP and thermal processing (TP). Spore suspensions (approximately 10(9) CFU/mL of water) of Clostridium tyrobutyricum, Bacillus sphaericus, and three strains of Bacillus amyloliquefaciens were treated by PATP (121 degrees C and 700 MPa) for 0, 10, 20, and 30 s and TP (121 degrees C) for 0, 10, 20, and 30 s. Treated and untreated spore suspensions were analyzed using FT-IR in the mid-infrared region (4000-800 cm(-1)). Multivariate classification models based on soft independent modeling of class analogy (SIMCA) were developed using second derivative-transformed spectra. The spores could be differentiated up to the strain level due to differences in their biochemical composition, especially dipicolinic acid (DPA) and secondary structure of proteins. During PATP changes in alpha-helix and beta-sheets of secondary protein were evident in the spectral regions 1655 and 1626 cm(-1), respectively. Infrared absorption bands from DPA (1281, 1378, 1440, and 1568 cm(-1)) decreased significantly during the initial stages of PATP, indicating release of DPA. During TP changes were evident in the bands associated with secondary proteins. DPA bands showed little or no change during TP. A correlation was found between the spore's Ca-DPA content and its resistance to PATP. FT-IR spectroscopy could classify different strains of bacterial spores and determine some of the changes occurring during spore inactivation by PATP and TP. Furthermore, this technique shows great promise for rapid screening PATP-resistant bacterial spores.  相似文献   
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Unpredictable drought affects growth and yield of dryland cowpea ( Vigna unguiculata [L.] Walp.) during rainy season. With the objective of identifying compensatory growth responses after relief of water stress, pot-grown plants (cv. C-752) were water-stressed at flowering, and physiological responses, short term dry matter partitioning upon relief of water stress, and productivity at maturity were studied. Water stress decreased, to varying degrees, leaf water potential, stomatal conductance, photosynthesis rate and transpiration rate. Recovery in assimilation lagged behind that in water relations. Assimilate supply seemed to be limiting early pod growth upon relief of water stress due to low photosynthesis rate, reduced leaf area per pod, and increased partitioning to leaf expansion. However, later pod growth was not limited by assimilate supply and final dry matter per pod was similar in both non-stressed and stress-affected plant. Cowpea exhibited the following growth responses during pod-fill stage upon relief of water stress: 1. increase in leaf area, 2. shift in dry matter partitioning in favour of leaf expansion, 3. extended green leaf duration, and 4. increase in pod number. These partially compensating physiological responses probably ensure reasonable productivity of dryland cowpea during rainy season.  相似文献   
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The earliest step in creating the cerebral cortex is the specification of neuroepithelium to a cortical fate. Using mouse genetic mosaics and timed inactivations, we demonstrated that Lhx2 acts as a classic selector gene and essential intrinsic determinant of cortical identity. Lhx2 selector activity is restricted to an early critical period when stem cells comprise the cortical neuroepithelium, where it acts cell-autonomously to specify cortical identity and suppress alternative fates in a spatially dependent manner. Laterally, Lhx2 null cells adopt antihem identity, whereas medially they become cortical hem cells, which can induce and organize ectopic hippocampal fields. In addition to providing functional evidence for Lhx2 selector activity, these findings show that the cortical hem is a hippocampal organizer.  相似文献   
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Comparison of the genomes and proteomes of the two diptera Anopheles gambiae and Drosophila melanogaster, which diverged about 250 million years ago, reveals considerable similarities. However, numerous differences are also observed; some of these must reflect the selection and subsequent adaptation associated with different ecologies and life strategies. Almost half of the genes in both genomes are interpreted as orthologs and show an average sequence identity of about 56%, which is slightly lower than that observed between the orthologs of the pufferfish and human (diverged about 450 million years ago). This indicates that these two insects diverged considerably faster than vertebrates. Aligned sequences reveal that orthologous genes have retained only half of their intron/exon structure, indicating that intron gains or losses have occurred at a rate of about one per gene per 125 million years. Chromosomal arms exhibit significant remnants of homology between the two species, although only 34% of the genes colocalize in small "microsyntenic" clusters, and major interarm transfers as well as intra-arm shuffling of gene order are detected.  相似文献   
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