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1.
A. Laarif S. Fattouch W. Essid N. Marzouki H. Ben Salah M. H. Ben Hammouda 《EPPO Bulletin》2003,33(2):335-338
In Tunisia, Phthorimaea operculella is an important pest in traditional, unrefrigerated potato stores. An integrated control approach including the use of biological control agents and specifically insect viruses is being developed. From this perspective, we carried out a screening and an evaluation of local Phthorimaea operculella granulosis virus naturally present in Tunisian pest populations. On the basis of symptomatology, P. operculella larvae were collected from different regions (North, Cap Bon, Sahel). Indirect ELISA using a serum raised against the purified Tunisian isolate of the virus was used to establish the geographical distribution of the virus. Comparison of the two approaches showed that symptomatology is not so virus-specific, as morphologically healthy insects were sometimes ELISA-positive. The serological technique therefore gives better detection of the virus. 相似文献
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Rekiki A Sidi-Boumedine K Souriau A Jemli J Hammami S Rodolakis A 《Veterinary research》2002,33(2):215-222
Chlamydiosis is one of the major diseases that can lead to abortion in ewes. Since 1997, in 5 regions of Tunisia, Chlamydia-related abortions have been reported in 15 sheep and goat flocks. One hundred and sixty-six sera and 50 vaginal swab samples were collected from adult ewes. Chlamydial antigens were detected in 29 (58%) of the vaginal swabs using Enzyme Linked Immunosorbent Assay (ELISA) while 9 (18%) were positive by cell culture. Five strains were recovered from 4 different sheep flocks. Monoclonal antibody profiles and restriction fragment length polymorphism (RFLP) analysis of the 16S-23S rRNA spacer region showed that these isolates were C. abortus. Using amplified fragment length polymorphism (AFLP), these Tunisian strains were shown to exhibit the same pattern as strains isolated in France. 相似文献
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Changes in follicular development of the ovary at 105, 140 and 175 d of age were observed on 48 prepuberal Yorkshire and Hampshire gilts raised on two planes of nutrition. The follicles from the left ovary were counted and measured by histological techniques. Both the nonatretic and the atretic (more than four pyknotic bodies) antral follicles were classified into six categories according to size, and each category was expressed as percentage of the total number. The proportion of nonatretic follicles belonging to the first category (.19 to .36 mm in diameter) was higher in Hampshire than Yorkshire gilts at d 105 (65.4 vs 47.6%; P less than .05), but not at d 140 and 175. The proportion of atretic follicles to the total number observed was higher in Yorkshire at d 175 than at d 140 and 105 (38.6 vs 21.9 vs 11.6%, respectively; P less than .05), whereas in Hampshire, significant differences were observed only between d 140 and 105 (25.2 vs 3.9%, respectively). The low-plane regimen reduced the percentage of nonatretic follicles of the third (.63 to 1.12 mm) and fourth (1.13 to 2.00 mm) categories in Yorkshire gilts from 14.4 and 7.2% to 9.7 and 4.0%, respectively (P less than .05). According to grouping, size and number of antral follicles, three morphological types of ovaries could be distinguished: honey-comb, grape-like and an intermediate class. Reanalyzing the data accounting for types resulted in radical reduction in the residual variation, hence many significant differences between the two breeds could be identified. At 140 and 175 d of age, Hampshires with honey-comb- and grape-like ovaries had greater numbers of medium and large follicles than Yorkshires (P less than .001). It is concluded that the slower follicular development observed at 105 d of age in Hampshire, as compared with Yorkshire gilts, allows accumulation of greater numbers of antral follicles at 140 and 175 d for both morphological types of ovaries. 相似文献
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【目的】研究筛选对芹菜叶斑病(病菌为:细极链格孢Alternaria tenuissima)具有良好防治作用的药剂。【方法】采用菌丝生长速率法和琼脂片法分别用14种杀菌剂对细极链格孢(A.tenuissima)菌丝和孢子萌发做室内毒力测定并进行田间药效试验。【结果】14种杀菌剂中对病原菌菌丝抑制效果最好的分别为3%甲霜恶霉灵、5%香芹酚、10%苯醚甲环唑、其EC50分别为10.8、19.9、26.2 μg/mL;对孢子萌发抑制效果最好的分别为3%甲霜恶霉灵、25%嘧菌酯、43%戊唑醇,其EC50分别为15.7、29.5、38.7 μg/mL。只有3%甲霜恶霉灵具有最佳的防治效果。防效最好的药剂为3%甲霜恶霉灵和5%香芹酚,其防效分别为96.3%和83.9%。【结论】3%甲霜恶霉灵最适用于田间芹菜叶斑病的防治。 相似文献
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Eight genotypes from two different genetic pools (high yielding varieties and landraces) were assessed for grain yield (GY) and for five quality traits: protein content (P), thousand kernel weight (TKW), yellow berry (YB), gluten content (Gc) and gluten index (Gi) in sub-humid and semi-arid areas using four combinations of nitrogen and potassium fertilizers during two cropping seasons. Genotype × Environment × Fertilizers (G × E × F) was significant only for protein content (p < 0.05); and E × F was found significant (p < 0.05) for all parameters. Greater quality related traits expression was noted in the semi-arid areas for both genetic pools. Excessive rainfall in semi-arid areas resulted in gluten elasticity reduction. N-fertilizers seemed to enhance protein content and to reduce thousand kernel weight. K-fertilizer, might enhance the increase in both proteins and thousand kernel weight in favorable growing conditions of water availability. Semolina yielding ability was higher in landraces as compared to high yielding varieties particularly using recommended fertilizer management. This group of cultivars showed superiority over high yielding cultivars for quantitative quality parameters. 相似文献
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Ahmed Amin Sayed ;Sobhy Ahmed Temerak ;Salah Mahmood Mohmed Gameel ;Amr Moussa 《农业科学与技术》2014,(6):529-534
Alternate two different modes of actions of green chemicals: spinosad & methoxyfenozide to combat the lesser date moth, Batrachedra amydraula and the Almond moth, Cadra cautella Walk on dates in Dakhla Oasis, New Valley, Egypt were done in 2010 and 201 I. Based on infestation of the early insect pest B. amydraula, the three-rotation program with basic 1st spray of spinosad 24 SC at 20 mL/100 L showed insignificant difference. Based on minimum effective rotation doses, alternating spinosad 24 SC at 20 mL/100 L, then methoxyfenozide 24 SC at 15 mL/100 L with two-week interval showed zero infestation and 1.8% at the last sample of 2010 and 2011, respectively. Based on infestation of the late insect pest, Cadra spp., tlhe same previous trend of the three-rotation results. Based on minimum effective rotation doses, alternating spinosad 24 SC at 20 mL/100 L, then methoxyfenozid 24 SC at 15 mL/100 L with three-week interval indicated less than 1% and 1% infestation during harvest for 2010 and 201 l, respectively. The last reflected important value when storing the fruit. Both products proved to be working under dry hot high temperatures. 相似文献
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The selective toxicity of new N-phosphorothioyl-carbamate esters 总被引:4,自引:0,他引:4
M A Fahmy T R Fukuto R O Myers R B March 《Journal of agricultural and food chemistry》1970,18(5):793-796