光照对箭筈豌豆及其子代生长和繁殖特性的影响     

杨凌杰  苟梦堃  布合利奇·马木提汗  胡小文  武艳培 《中国草地学报》2021,(2)

光照不仅会影响植物自身的生长,也可能会对其子代的生长具有潜在作用。以箭筈豌豆品种兰箭1号和兰箭3号为材料,分析遮荫和自然光照下箭筈豌豆及其子代的生长特性。结果表明:光照对箭筈豌豆生长和繁殖特性的影响因基因型而异。与光照条件相比,遮荫显著降低兰箭1号生物量及茎叶比,但对繁殖分配的比例影响不显著。与此相反,遮荫对兰箭3号的生长与繁殖无显著影响;母本光照环境对箭筈豌豆子代生长特性具有一定程度的影响,且这种影响因子代植株生长的光照环境而异。当子代在遮荫环境下,母本环境显著影响兰箭1号分枝数、地上生物量、总生物量、地上/地下生物量以及兰箭3号的分枝数。但当子代在光照环境下生长时,除兰箭3号分枝数外,其他生长特性均不受母本环境影响。  相似文献   

Tanshinone ⅡA induces apoptosis via JNK signaling pathway in human osteosarcoma HOS cells     

LU Jian-hong  HUANG Xiao-wen  JIN Yi-jun  YANG Yan-nan  WU Yi-zheng 《园艺学报》2019,35(1):28-33

AIM: To explore the effect of tanshinone ⅡA on human osteosarcoma HOS cells and the underlying mechanism.METHODS: The cell viability and the appropriate dose of tanshinone ⅡA were determined by CCK-8 assay. Colony formation assay and Transwell assay were used to investigate the proliferation and migration abilities of the HOS cells treated with tanshinone ⅡA. The apoptosis of the HOS cells was monitored by Hoechst 33258 staining, transmission electron microscopy and flow cytometry. The protein levels of apoptosis-related molecules and JNK signaling-associated proteins were determined by Western blot. Meanwhile, a JNK inhibitor was added for confirming the relationship between the pathway and apoptosis mentioned above.RESULTS: Tanshinone ⅡA inhibited both HOS cell proliferation and migration in a dose-and time-dependent manner. Exposure of the HOS cells to tanshinone ⅡA resulted in the activation of apoptosis. Tanshinone ⅡA treatment increased the protein levels of cleaved caspase-3, Bax and JNK signaling-associated proteins, and decreased the protein level of Bcl-2, which were reversed by JNK inhibitor SP600125. Moreover, the result of CCK-8 assay revealed that tanshinone ⅡA-induced cell death was alleviated by JNK inhibitor.CONCLUSION: Tanshinone ⅡA induces cell growth inhibition and the activation of apoptosis via JNK signaling pathway in human osteosarcoma HOS cells.  相似文献   

叶蛋白研究进展     

高琦  金小乂  刘梓蘅  王晓文  张俊伟  薛友林 《保鲜与加工》2020,20(1):232-236

叶蛋白是一类从植物叶片中提取的蛋白质,一般其最终蛋白质得率占总叶蛋白的40%~60%,其脂肪含量低且不含胆固醇,是一类具有较高的营养价值和保健功能的蛋白质。本文概述了叶蛋白的多种来源,介绍了7种叶蛋白的提取方法,并对叶蛋白的脱毒、脱色、脱腥及营养价值进行了分析。同时还简述了叶蛋白在饲用、食用和医用方面的应用,以期为读者提供叶蛋白研究的最新进展。  相似文献   

Cloning and Expression Analysis of BoSPx in Brassica oleracea     

WANG YuKui  BAI XiaoJing  LIAN XiaoPing  ZHANG HeCui  LUO ShaoLan  PU Min  ZUO TongHong  LIU QianYing  ZHU LiQuan 《中国农业科学》2018,51(22):4328-4338

  相似文献   

水保综合治理已成为新县经济发展的重要支撑     

连光学 《中国水土保持》2006,(8):42-44

新县是一个集“老、边、贫”为一体的国家级山区贫困县,水土流失面积达1 411 km2,治理前年均土壤侵蚀模数达3 340 t/km2。截至2002年,全县初步完成水土保持综合治理面积751 km2,年均土壤侵蚀模数下降到1 250 t/km2。通过综合治理,新增有效灌溉面积12.6万亩,新增旱涝保收田8.4万亩,兴修道路600多km,农村解决人畜饮水5 000多处,75%的农户用上了自来水,65%的农户翻盖了砖瓦房,极大地改变了农村面貌,已有90%以上的农民解决了温饱、摆脱了贫困,开始走向致富道路。针对存在的问题,提出了加快该县水土保持事业发展的建议。  相似文献   

闭锁群体BLUP选择下遗传方差和近交系数的变化     

鲁绍雄  连林生  李明丽 《畜牧与兽医》2004,36(8):13-15

采用计算机随机模拟方法模拟了在一个闭锁群体内连续对单个性状进行 1 5个世代选择的情况。选择过程中世代不重叠 ,每个世代的种畜根据动物模型最佳线性无偏预测 (BLUP)法估计的育种值进行选留 ,并在此基础上系统地比较了不同群体规模、公母比例和性状遗传力对群体遗传方差和近交系数变化的影响。结果表明 ,扩大育种群规模、增加公畜比例以及对低遗传力性状进行选择时 ,群体遗传方差降低的速度和近交系数上升的速度会更慢 ,在长期选择时可望获得更大的持续进展和适宜的近交增量  相似文献   

Antitumor effect of chlorophyllin in vitro     

DING Xiao-wen  ZHENG Shu  PENG Jia-ping  LV Qing-hua  HUANG Jian 《园艺学报》2004,20(9):1659-1663

AIM: To study the effect and mechanism of chlorophyllin (CHL) inhibiting HT29 cells. METHODS: IC₅₀ value and growth curve of HT29 cells were detected with MTT method. Apoptosis was detected with Wright-Giemsa staining, FCM and DNA electrophoresis. Telomerase was detected by PCR-ELISA, and protein and mRNA expression of COX-2 gene were detected through RT-PCR and Western blot. RESULTS: CHL inhibited the growth of HT29 in a dose-dependent manner. CHL blocked HT29 cells in G₁ phase but did not induce apoptosis. Different concentration of CHL inhibits the expression of telomerase and COX-2 in HT29 cells. CONCLUSION: CHL inhibited the growth of HT29 cells by inhibiting the expression of telomerase and COX-2 and blocking cells in G₁ phase.  相似文献   

Effects of non-excitatory electric stimulation on cardiac function in normal and infarcted heart rabbits and it's regional effect on myocardium     

ZHANG Hai-zhu  CUI Chang-cong  ZHAO Xiao-jing  LEI Li-quan  LIAN Jiang-fang 《园艺学报》2004,20(10):1846-1848

AIM: To investigate the influences of electric stimulation applied during the absolute refractory period (ARP) on the cardiac function of normal rabbits and rabbits after myocardial infarction (MI) and to observe the regional effects of this electric stimulation. METHODS: 64 rabbits were randomly assigned to normal and MI groups and each group was then divided into the anterior and posterior groups. A thoracotomy was performed 4 weeks after MI in rabbits. One set of electrodes was inserted into the anterior and posterior wall of left ventricle of the anterior and posterior groups, respectively. Current pulses were delivered during the ARP (called CCM) during sinus rhythm in rabbits. The left ventricular systolic pressure (LVSP) and the left ventricular end diastolic pressure(LVEDP) as well as maximum positive and negative left ventricular pressure change (±dp/dt_max) were observed. RESULTS: In the normal and MI groups, LVSP, +dp/dt_max significantly increased, and LVEDP, -dp/dt_max were reduced during CCM stimulation compared with the baseline (P<0.05). In the normal rabbits, electric stimulation in the anterior group improved the cardiac function more significantly than that in the posterior group (P<0.05). In the MI rabbits, there was no difference between the anterior and the posterior groups (P>0.05). CONCLUSION: Electric stimulation delivered during the ARP significantly enhances the contractility and the relaxation of myocardium in normal rabbits and rabbits after MI, and the effects of CCM stimulation on heart are regional.  相似文献   

鼠层粘蛋白α5链E3、LG4、LG5片段的克隆及表达     

孙兆增  张玉静  展德文  张艳宇  连继勤 《中国兽医学报》2004,24(5):467-469

从鼠的肝脏组织中提取总 RNA,采用 RT- PCR获得了鼠层粘蛋白 ( laminin)α5链的 E3 ( L G4 - 5)、L G4 和 L G5等 3个基因片段 ,与 NCBI数据库参考序列相比 ,同源性在 99%以上。将 E3 ( L G4 ,5)、L G4 和 L G5定向克隆到原核高效表达载体 Pet- 2 8a中 ,构建 Pet- 2 8a- E3 、Pet- 2 8a- L G4 、Pet- 2 8a- L G5等 3个原核表达质粒。将表达质粒转化表达受体菌 BL2 1中 ,IPTG诱导表达。收集菌液进行 SDS- PAGE电泳、Western- blotting分析 ,结果显示 ,这 3段基因在原核细胞中成功表达 ,薄层扫描显示表达蛋白占细胞总蛋白的 2 0 %以上。大量提取包涵体 ,纯化后免疫家兔 ,8周后得多克隆抗体 ,间接 EL ISA在抗体稀释到 1∶ 1 2 80 0时仍为阳性  相似文献   

以催乳素受体基因作为撒坝猪部分生产性能候选基因的研究   总被引：8，自引：0，他引：8  

李国治  连林生  鲁绍雄  严达伟 《养猪》2005,(4):36-38

此文运用PCR-RFLP技术检测催乳素受体(PRLR)基因在撒坝猪群体中的多态性分布,并采用最小二乘分析模型初步统计分析基因多态性与部分生产性能的相关性。结果表明,PRLR基因等位基因B及其基因型BB在群体中占优势,频率分别为0.6594和0.4438;PRLR基因在群体中处于Hardy-Weinberg平衡状态;头胎繁殖性状有利等位基因B对生长性状无不利影响。  相似文献