全文获取类型
| 收费全文 | 319篇 |
| 免费 | 32篇 |
| 国内免费 | 1篇 |
专业分类
| 林业 | 17篇 |
| 农学 | 8篇 |
| 基础科学 | 1篇 |
| 32篇 | |
| 综合类 | 47篇 |
| 农作物 | 8篇 |
| 水产渔业 | 51篇 |
| 畜牧兽医 | 159篇 |
| 园艺 | 18篇 |
| 植物保护 | 11篇 |
出版年
| 2023年 | 2篇 |
| 2022年 | 3篇 |
| 2021年 | 7篇 |
| 2020年 | 14篇 |
| 2019年 | 9篇 |
| 2018年 | 17篇 |
| 2017年 | 16篇 |
| 2016年 | 10篇 |
| 2015年 | 13篇 |
| 2014年 | 14篇 |
| 2013年 | 12篇 |
| 2012年 | 11篇 |
| 2011年 | 26篇 |
| 2010年 | 18篇 |
| 2009年 | 13篇 |
| 2008年 | 17篇 |
| 2007年 | 19篇 |
| 2006年 | 24篇 |
| 2005年 | 19篇 |
| 2004年 | 14篇 |
| 2003年 | 11篇 |
| 2002年 | 20篇 |
| 2001年 | 3篇 |
| 2000年 | 2篇 |
| 1999年 | 6篇 |
| 1998年 | 6篇 |
| 1997年 | 3篇 |
| 1994年 | 1篇 |
| 1993年 | 2篇 |
| 1992年 | 2篇 |
| 1991年 | 2篇 |
| 1990年 | 2篇 |
| 1989年 | 5篇 |
| 1988年 | 1篇 |
| 1987年 | 1篇 |
| 1986年 | 1篇 |
| 1985年 | 1篇 |
| 1976年 | 1篇 |
| 1974年 | 1篇 |
| 1972年 | 1篇 |
| 1967年 | 1篇 |
| 1966年 | 1篇 |
排序方式： 共有352条查询结果，搜索用时 31 毫秒
1.
Melody L. Danley P. Brett Kenney Patricia M. Mazik Rodney Kiser Joseph A. Hankins 《Journal of the World Aquaculture Society》2005,36(3):249-261
Rainbow trout Oncorhynchus mykiss (261.6 × 24.7 g initial weight, mean × SEM) at 13.1 × 0.2 C were exposed for 94 d to one of three CO2 treatments: control (22.1 × 2.8 mg/L), medium (34.5 × 3.8 mg/L), or high (48.7 × 4.4 mg/L). Trout were checked daily for survival, and fish were sampled at 0, 28, 56, and 84 d for physiological responses, growth, and fillet quality assessments. Trout were also challenged to a 15-min crowding stress at 93 d to assess their ability to initiate a stress response during hypercapnia. Chronically exposed trout showed nearly 100% survival through 84 d exposure (1 of 1,500 fish died). Growth and physiological results showed that increasing elevated CO2 , concentrations result in corresponding decreased growth rates and CO2 specific physiological parameters: The medium and high CO2 treatments had significantly slower growth and subsequently smaller fish by 84 d. Exposed trout also showed significantly ( P < 0.05) decreased plasma chloride for medium and high CO2 treatments compared to the control from 28 through 84 d. Decreased growth and smaller fish in the medium and high CO2 treatments resulted in correspondingly smaller fresh and smoked fillet weights. Chronic CO2 exposure did not result in notable changes in ultimate muscle pH. Exposure to 15-min crowding stress at 93 d resulted in significant changes in hematocrit, plasma cortisoI, glucose, and chloride for all treatment groups. CO2 -specific changes were detected in hematocrit, plasma cortisoI, and plasma chloride responses following the 15-min crowding stress. 相似文献
2.
John F. Leslie Kurt A. Zeller Melissa Wohler Brett A. Summerell 《European journal of plant pathology / European Foundation for Plant Pathology》2004,110(5-6):611-618
Gibberella fujikuroi and Gibberella intermedia(mating populations ‘C’ and ‘D’ of the G. fujikuroi species complex) can be distinguished by differences in the spectrum of mycotoxins produced, the lack of sexual cross-fertility and diagnostic differences in their DNA sequences. Some isolates from these two biological species, however, can interbreed and complete meiosis to produce viable progeny. Analysis of marker segregation amongst such hybrid progeny can be used to estimate the degree of genomic rearrangement and genetic incompatibility that has accumulated since these sibling species diverged. Recombinant progeny were isolated from crosses of the standard tester strains for these two species and from crosses between these standard testers and a field isolate (KSU X-10626) that was cross-fertile with tester strains of both species. Progeny in all of the crosses segregated for amplified fragment length polymorphisms (AFLPs). Segregation of AFLP loci deviated from 1:1 for two thirds of the loci amongst the progeny of the cross between the ‘C’ and ‘D’ mating population tester strains, but <20% of the polymorphic loci in the cross of either tester with KSU X-10626 showed such distortion. It was concluded that G. intermedia and G. fujikuroi are sufficiently interfertile to belong to the same biological species, but that changing the nomenclature to reflect this interfertility requires more evidence for the natural occurrence of a continuum in fertility than is presently available. 相似文献
3.
Dennis N. Lozada R. Esten Mason Md Ali Babar Brett F. Carver Gina-Brown Guedira Keith Merrill Maria Nelly Arguello Andrea Acuna Lucas Vieira Amanda Holder Christopher Addison David E. Moon Randal G. Miller Susanne Dreisigacker 《Euphytica》2017,213(9):222
Genome-wide association studies (GWAS) are useful to facilitate crop improvement via enhanced knowledge of marker-trait associations (MTA). A GWAS for grain yield (GY), yield components, and agronomic traits was conducted using a diverse panel of 239 soft red winter wheat (Triticum aestivum) genotypes evaluated across two growing seasons and eight site-years. Analysis of variance showed significant environment, genotype, and genotype-by-environment effects for GY and yield components. Narrow sense heritability of GY (h 2 = 0.48) was moderate compared to other traits including plant height (h 2 = 0.81) and kernel weight (h 2 = 0.77). There were 112 significant MTA (p < 0.0005) detected for eight measured traits using compressed mixed linear models and 5715 single nucleotide polymorphism markers. MTA for GY and agronomic traits coincided with previously reported QTL for winter and spring wheat. Highly significant MTA for GY showed an overall negative allelic effect for the minor allele, indicating selection against these alleles by breeders. Markers associated with multiple traits observed on chromosomes 1A, 2D, 3B, and 4B with positive minor effects serve as potential targets for marker assisted breeding to select for improvement of GY and related traits. Following marker validation, these multi-trait loci have the potential to be utilized for MAS to improve GY and adaptation of soft red winter wheat. 相似文献
4.
Context
North American grassland songbird populations have declined significantly due to habitat loss and fragmentation. Understanding the influence of the surrounding landscape on prairie fragment occupancy is vital for predicting the fate of grassland birds in these heavily altered landscapes.Objectives
We examined the relative importance of local and landscape variables on grassland bird occupancy of prairie fragments using a focal-patch study. We also investigated the spatial scale at which landscape variables were most influential.Methods
We surveyed birds on 29 unplowed prairie fragments in western Minnesota and eastern North and South Dakota. We quantified local habitat on the fragment using vegetation surveys and aerial photographs and the landscape surrounding the fragment out to 4 km using aerial photographs. We analyzed occupancy using multi-model approaches applied to multiple logistic regression.Results
Of 38 species encountered, nine were neither too rare nor too abundant to be analyzed. Predictors of patch occupancy were unique for each bird species, yet general patterns emerged. For eight species, landscape variables were more important than local variables. Mostly, those landscape variables measured configuration (e.g., edge density) and not composition (e.g., percent cover of a particular matrix element). Landscape effects were mostly from variables measured at the greatest extents from the prairie fragment.Conclusions
Using a focal-patch study design we demonstrated the importance of the surrounding landscape, often out to 4 km from the fragment edge, on prairie occupancy by grassland birds. Effective management of grassland songbirds will require attention to the landscape context of prairie fragments.5.
Flow alteration and thermal pollution depress modelled growth rates of an iconic riverine fish,the Murray cod Maccullochella peelii 
下载免费PDF全文
下载免费PDF全文 Nick S. Whiterod Shaun N. Meredith Paul Humphries Bradford S. Sherman John D. Koehn Robyn J. Watts Brett A. Ingram Tom Ryan 《Ecology of Freshwater Fish》2018,27(3):686-698
The serial discontinuity concept (SDC) proposes that hypolimnetic‐releasing impoundments cause major disruptions to the naturally occurring physical, chemical and biological gradients of rivers but that this impact diminishes with distance downstream. Such a gradient in discharge, flow velocity and temperature regime occurs below a large hypolimnetic‐releasing impoundment, the Hume Dam, on the River Murray in south‐eastern Australia. To examine the effects of this disturbance gradient on a warm‐water large‐bodied freshwater fish, the Murray cod (Maccullochella peelii), a bioenergetics model was developed and calibrated to explore energy expended under differing water velocities and temperature regimes. Model simulations predicted negative growth of juveniles directly downstream of the impoundment, due largely to the energetic costs associated with active and, to a lesser extent, standard metabolism outweighing the achievable energetic gains through food consumption. As flow velocity and temperature regimes became more favourable downstream, so did the simulated growth of the species. It was not until +239 km downstream of the impoundment that the model predicted that flow velocity and temperature regimes were suitable for greater weight gains. The modelled growth responses of juvenile Murray cod are consistent with the predictions of the SDC, emphasising that changes in the bioenergetics of individuals are likely to be reflected in reduced growth rates under the changed flow velocity and temperature regimes imposed by disturbance gradients. This research represents a valuable step in the biological understanding of Murray cod within variable riverine environments and emphasises the urgency required to mitigate impacts associated with hypolimnetic impoundments. 相似文献
6.
7.
8.
9.
10.
Jrme Van Biervliet DVM Dipl ACVIM Marc Kraus DVM Dipl ACVIM Brett Woodie DVM Dipl ACVS Thomas J. Divers DVM Dipl ACVIM Dipl ACVECC Anna Gelzer Dr. Med. Vet. Dipl ACVIM Dorothy Ainsworth DVM PhD Dipl ACVIM 《Journal of Veterinary Cardiology》2006,8(1):69-73
A 5-year-old Holstein cow, pregnant with a valuable calf, was presented with signs of heart failure (tachycardia, peripheral edema, and distended jugular veins) related to pericardial lymphoma and associated cardiac tamponade. In addition, pleural effusion was present in both hemithoraces. Medical treatment, which consisted of repeated pericardiocenteses, placement of indwelling pleural catheters, administration of intravenous fluid therapy, antibiotics and anti-inflammatory drugs, was ineffective in controlling recurrence of clinical signs despite a temporary improvement. A standing thoracoscopic pericardiotomy was performed in an attempt to reduce clinical signs of heart failure and to prolong life. Clinical signs of heart failure abated and no recurrence was seen. Standing thoracoscopic pericardiotomy along with possible corticosteroids can be recommended as palliative treatment in an effort to extend life for reproductive performance in genetically valuable animals. 相似文献