<Emphasis Type="Italic">Lupinus albus</Emphasis> Conglutin Gamma Modifies the Gene Expressions of Enzymes Involved in Glucose Hepatic Production <Emphasis Type="Italic">In Vivo</Emphasis>     

Ana E. González-Santiago  Belinda Vargas-Guerrero  Pedro M. García-López  Alma L. Martínez-Ayala  José A. Domínguez-Rosales  Carmen M. Gurrola-Díaz 《Plant foods for human nutrition (Dordrecht, Netherlands)》2017,72(2):134-140

Lupinus albus seeds contain conglutin gamma (Cγ) protein, which exerts a hypoglycemic effect and positively modifies proteins involved in glucose homeostasis. Cγ could potentially be used to manage patients with impaired glucose metabolism, but there remains a need to evaluate its effects on hepatic glucose production. The present study aimed to analyze G6pc, Fbp1, and Pck1 gene expressions in two experimental animal models of impaired glucose metabolism. We also evaluated hepatic and renal tissue integrity following Cγ treatment. To generate an insulin resistance model, male Wistar rats were provided 30% sucrose solution ad libitum for 20 weeks. To generate a type 2 diabetes model (STZ), five-day-old rats were intraperitoneally injected with streptozotocin (150 mg/kg). Each animal model was randomized into three subgroups that received the following oral treatments daily for one week: 0.9% w/v NaCl (vehicle; IR-Ctrl and STZ-Ctrl); metformin 300 mg/kg (IR-Met and STZ-Met); and Cγ 150 mg/kg (IR-Cγ and STZ-Cγ). Biochemical parameters were assessed pre- and post-treatment using colorimetric or enzymatic methods. We also performed histological analysis of hepatic and renal tissue. G6pc, Fbp1, and Pck1 gene expressions were quantified using real-time PCR. No histological changes were observed in any group. Post-treatment G6pc gene expression was decreased in the IR-Cγ and STZ-Cγ groups. Post-treatment Fbp1 and Pck1 gene expressions were reduced in the IR-Cγ group but increased in STZ-Cγ animals. Overall, these findings suggest that Cγ is involved in reducing hepatic glucose production, mainly through G6pc inhibition in impaired glucose metabolism disorders.  相似文献   

Assessing seed desiccation responses of native trees in the Caribbean     

Mattana  Efisio  Peguero  Brígido  Di Sacco  Alice  Agramonte  Wilvin  Encarnación Castillo  Wilkin Rafael  Jiménez  Francisco  Clase  Teodoro  Pritchard  Hugh W.  Gómez-Barreiro  Pablo  Castillo-Lorenzo  Elena  Terrero Encarnación  Marianny  Way  Michael J.  García  Ricardo  Ulian  Tiziana 《New Forests》2020,51(4):705-721

New Forests - Native trees from the Caribbean were tested for seed desiccation responses, by adapting the “100-seed test” protocol. Ninety-seven seed lots of 91 species were collected...  相似文献   

Prediction of Genetic Gain in Sweet Corn using Selection Indexes     

da Silva  Isadora Gonçalves  Castoldi  Renata  de Oliveira Charlo  Hamilton César  de Souza Miranda  Mateus  Nunes  Thaíssa Dias Cardoso  Costa  Luciene Lacerda  Lemes  Ernane Miranda 《Journal of Crop Science and Biotechnology》2020,23(2):191-196

Journal of Crop Science and Biotechnology - The cultivation of sweet corn is expanding in Brazil, but there are serious constraints about the availability of commercial cultivars. The selection of...  相似文献   

Backshoring and nearshoring: An overview     

Benedetta Piatanesi  Josep‐Maria Arauzo‐Carod 《Growth and change》2019,50(3):806-823

Increasingly, economies that have traditionally benefited from offshoring are losing some of their strategical advantages, with a consequent increase in backshoring by developed economies. This paper describes the phenomenon and tries to shed light on the current challenges, trends, and debates in the area, and on the main determinants of backshoring. A new phenomenon known as nearshoring is also analysed—this consists of relocating some previously offshored manufacturing activities so that they are now close to previous core locations, but not so close as to suffer from disagglomeration effects. This combines the advantages of offshoring and backshoring.  相似文献   

Skeletal muscle fiber size in untrained and endurance-trained horses.     

J L López-Rivero  E Agüera  J G Monterde  J Vivo  M V Rodríguez-Barbudo 《American journal of veterinary research》1992,53(5):847-850

The mean area and minimal diameter of 3 histochemically determined myofiber types (1, 2A, and 2B; myosin ATPase in acid buffer) were calculated in middle gluteal muscle biopsy specimens from 62 stallions, 47 Andalusians and 15 Arabians, ranging in age from 6 to 12 years. Fourteen Andalusians and 7 Arabians were untrained, and the remainder were actively endurance-trained. The 6-month training schedules involved walking, slow trotting, and cantering. Fourteen Andalusians were moderately endurance-trained, whereas the other 19 Andalusians and 8 Arabians were strongly endurance-trained. Significant differences were not recorded between untrained and endurance-trained Arabians with respect to the area (type 1, 3,194 +/- 869 microns 2 and 3,150 +/- 370 microns 2; type 2A, 3,819 +/- 890 microns 2 and 3,380 +/- 356 microns 2; and type 2B, 4,872 +/- 962 microns 2 and 4,417 +/- 646 microns 2) or minimal diameter (type 1, 52.2 +/- 7.4 microns and 52.8 +/- 3.1 microns; type 2A, 58.1 +/- 6.7 microns and 55.0 +/- 2.8 microns; and type 2B, 65.3 +/- 6.4 microns and 63.4 +/- 4.3 microns) of the 3 fiber types, nor between untrained and endurance-trained Andalusians with respect to the area (untrained, 3,990 +/- 690 microns 2; moderately endurance-trained, 3,882 +/- 347 microns 2; and strongly endurance-trained, 3,758 +/- 510 microns 2) and minimal diameter (untrained, 58.1 +/- 4.7 microns; moderately endurance-trained, 59.7 +/- 2.7 microns; and strongly endurance-trained, 58.7 +/- 4.5 microns) of 2A fibers.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

Antibodies to bovine serum albumin in swine sera: implications for false-positive reactions in the serodiagnosis of African swine fever   总被引：2，自引：0，他引：2  

J M Escribano  M J Pastor  J M Sánchez-Vizcaíno 《American journal of veterinary research》1989,50(7):1118-1122

Antibodies to bovine serum albumin were detected in swine sera by use of an immunoblotting technique. Such sera had false-positive reactions, as determined by results of African swine fever virus serodiagnostic techniques when bovine serum albumin was a contaminant in the soluble cytoplasmic antigen obtained from infected cells cultured in the presence of bovine serum. The soluble cytoplasmic antigen obtained from cell cultures infected with African swine fever virus in the presence of porcine serum did not react with the false-positive sera and, therefore, was used for African swine fever virus serodiagnostic methods, with 0% false-positive results.  相似文献   

Divergent selection for shape of growth curve in Japanese quail. 1. Responses in growth parameters and food conversion.     

L. Hyánková  H. Knížetová  L. Dědková  J. Hort 《British poultry science》2001,42(5):583-589

1. HG and LG quail lines selected for high and low relative weight gain between 11 and 28 d of age (RG11-28), respectively, and an unselected C line were compared. Mature body weight of both selected lines was held at that of the C line. Progeny of generation 6 were used for analysis. 2. Divergent selection for RG11-28 brought about opposite changes in the growth rates shortly after hatching. 3. Parameters of the Richards function were used to describe the growth curve. The largest differences between HG and LG lines occurred in age (t+) and body weight (y+) at the inflection point of the growth curve (on average for both sexes 28% and 20%, respectively). For HG quail, the parameter t+ was 5 d later than that for LG quail (18.6 vs 14.1 d for males and 20.6 vs 15.6 d for females, respectively), and consequently the parameter y+ was greater (90.3 vs 84.0 g for males and 104.5 vs 96.1 g for females, respectively). The shape of the growth curve expressed by the y+/A ratio was substantialy different for HG and LG quail (44.8% vs 39.6% for males and 43.5% vs 36.8% for females, respectively). 4. The food/gain ratios for the fattening period (3 to 35 d of age) were 3.21, 3.47 and 3.34 for the HG, LG and C lines, respectively. The HG quail started to utilise food more efficiently than the LG quail as early as 10 to 14 d, that is, at the age when their relative growth rate first became greater. 5. The relative deviations of the HG and LG lines from the C line are discussed.  相似文献   

ULTRASONOGRAPHIC LOCALIZATION OF A CAUDAL VENA CAVA THROMBUS IN A DOG WITH LEISHMANIASIS     

Artur  Font  DVM  Josep M.  Closa  DVM 《Veterinary radiology & ultrasound》1997,38(5):394-396

A dog with visceral leishmaniasis developed rear limb edema, and distension of the caudal epigastric veins. Glomerular disease with nephrotic syndrome and hypercoagulable state was diagnosed. Sonographically there was massive thrombosis of the caudal vena cava.  相似文献   

Experimental and natural infection with the enzootic leukosis virus of cattle     

B Hofírek  P Horín  M Granátová  M Machatková  J Franz  I Svoboda  J Blecha 《Veterinární medicína》1986,31(3):129-140

A trial was performed with heifers at the age of six to seven months. The animals were experimentally infected with the lymphocytes of a virus-productive donor. Infection was produced in all the nine cases, as demonstrated by means of the positive syncytial test. As indicated by the results of the trial, the antibodies to the enzootic bovine leucosis virus (BLV) were produced soon after experimental infection. A high sensitivity of the serum-neutralization test and the ELISA method was demonstrated in this connection: by these methods, the antibodies were identified already two to three weeks after experimental infection whereas by the immunodiffusion test they could be detected only after five weeks. Twenty-four animals were exposed to natural contact infection. Within 270 days of the trial, the disease after contact was recorded only in one heifer out of the four that were in close contact with the experimentally infected animals. In this case, as compared with experimental infection, the antibodies were produced much later--after 85 to 93 days. Leucosis was recorded in none of the remaining animals. The reasons why such a favourable result was obtained were the thorough disinfection of the stables after blood collections and the strict observance of the aseptic conditions. The results of experimental infection in three cows were identical with those obtained in young cattle. In the experimentally infected dairy cows, antibodies in milk were determined by the ELISA method. As found, in milk the antibodies to BLV appear two to three weeks later than they do in serum. The ELISA method of BLV antibody detection can be used for the identification of infected animals in herds where enzootic bovine leucosis occurs.  相似文献   

Mucus synthesis in the goblet cells of the small intestine in experimental infection with the coccidium Isospora suis in piglets     

M Kudweis  Z Lojda  B Koudela  J Vítovec 《Veterinární medicína》1989,34(12):727-734

The state of mucus synthesis in the goblet cells of the small intestine was studied in conventional piglets infected with a dose of 200,000 oocytes of the coccidium Isospora suis the first and fifth day after parturition. The synthesis of mucus and its chemical characteristics undergo significant changes during the third and fourth day after infection. The activity of acid and neutral mucous substances declines; their level and the physiological synthetic function of goblet cells begin to return to the normal during the period starting on the eight to tenth day after infection. However, there were no fully functioning goblet cells in the broken numerical ratio even at the end of the period of investigation, i.e. the 13th day after infection. The thin surface layer of mucus remained almost unchanged within the whole extent of the small intestine parts studied.  相似文献