A recombinant-based feline immunodeficiency virus antibody enzyme-linked immunosorbent assay.     

B Mermer  P Hillman  R Harris  T Krogmann  Q Tonelli  W Palin  P Andersen 《Veterinary immunology and immunopathology》1992,35(1-2):133-141

We have developed an antibody detection enzyme-linked immunosorbent assay (ELISA) for the identification of animals infected by feline immunodeficiency virus (FIV). The ELISA solid-phase antigen consists of recombinant FIV gag proteins expressed in bacteria. The proteins are purified from bacterial lysates as insoluble inclusion bodies. In the case of bacterially expressed p24gag, it is shown that all of the linear, sequential epitopes presented by viral p24 during infection are retained. Purified preparations can be substituted for solid-phase whole virus in the IDEXX PetChektm immunoassay. The antibody ELISA duplicates the sensitivity and specificity of the whole virus based PetChek plate assay.  相似文献   

Artificial Insemination with Frozen Semen in Ewes at Different Times of the Breeding Season     

K. Andersen Berg  J. Aamdal 《Reproduction in domestic animals》1991,26(1):27-30

Totally 13575 ewes of two different breeds, Dala and Spel, were inseminated with semen, frozen in straws and thawed at 70°C for 8 sec. An insemination dose of 0.2 ml containing approx. 150 × 10⁶ spermatozoa with at least 45 to 50% progressive motility was imerted 5 to 12 mm into the cervix. The insemination was performed once between 12 and 30 h after the onset of heat. The NR rate of the Dala ewes increased significantly during the season. The NR rate of the ewes inseminated before 15. November was 44.3%, from 15. to 20. November 52.2%. from 20. to 25. November 55.3% and from 25. November and later 61.4%. The corresponding values for the ewes of the Spel breed were 57.3, 58.7, 61.5 and 71.0% respectively, and only the difference between the two last values was statistically significant. The difference between the fertility of the two breeds was significant within each of the periods .  相似文献   

Cross reaction between bovine enterovirus and South African Territories I5 foot-and-mouth disease virus.     

A A Andersen 《American journal of veterinary research》1978,39(1):59-63

A bovine enterovirus (E76T) isolated from a 2-year-old bull produced serologic cross reactions to South African Territories (SAT) I5 foot-and-mouth disease virus when inoculated into guinea pigs and cattle. Cross-reacting serum titers to SAT I5 virus of 1:320 by the plaque-reduction neutralization test and 1:20 by the radial immunodiffusion test occurred in 2 steers after they were inoculated with the E76T virus. In 1 steer, maximal cross-reacting titers appeared related to a 2nd exposure to the viruses or to a hyperimmune state. Ultracentrifugation and 2-mercaptoethanol studies indicated that the cross reactions were due to immunoglobulin M antibody. Sera from guinea pigs immunized with the E76T or the SAT I5 virus cross reacted with the heterologous virus by postinoculation day 7. Cross-reacting titers had decreased markedly by postinoculation day 35, whereas the homologous virus titer remained constant. Cross reactivity of the E76T virus was primarily with the SAT I5 virus, and to a lesser degree with SAT II3. Cross reactions did not occur with representatives of the 5 other antigenic types of foot-and-mouth disease virus.  相似文献   

Cross reactions of normal bovine sera with foot-and-mouth disease virus: incidence, duration, and effect of shipping stress.     

A A Andersen 《American journal of veterinary research》1978,39(4):603-606

Serum samples were obtained from 30 Hereford steers in an area known to be free of foot-and-mouth disease (FMD) viruses as follows: before shipment and 4 times during a 70-day period after shipment; the sera were tested for the presence of cross-reacting antibody to various viruses. Percentages of sera containing cross-reacting antibody to FMD virus detected by the plaque-reduction neutralization and the radial immunodiffusion techniques were higher for the FMD viruses Asia and SAT I5 than for the FMD viruses A5, O1, and C1. Cross-reacting antibody was usually of low titer and usually present in only 1 or 2 consecutive serum samples. The incidence of cross reactions increased after stress of shipping and thus an infective agent may be responsible. These results were compared with results from sera collected from Herefords and Holstein-Friesians in a 2nd area; results did not indicate that Herefords have an excess of cross reactions with FMD viruses.  相似文献   

Identification of Chlamydophila pneumoniae in an emerald tree boa, Corallus caninus.     

Elliott R Jacobson  Darryl Heard  Arthur Andersen 《Journal of veterinary diagnostic investigation》2004,16(2):153-154

Tissues were evaluated from emerald tree boas, Corallus caninus, from a collection in which chlamydiosis was diagnosed. To determine the strain of chlamydia infecting these snakes, tissue samples from 5 frozen snakes were tested by a quantitative TaqMan polymerase chain reaction (PCR) test and a PCR sequence analysis test. Of the 22 samples tested, 9 were categorized as either positive or weakly positive with the TaqMan test, and 6 yielded an amplicon using a serial PCR test that amplified a portion of the 23S ribosomal RNA gene. A PCR product suitable for sequencing was obtained from the heart of one of the snakes. Sequence analysis showed that the snake had been infected with Chlamydophila pneumoniae. These findings show that C. pneumoniae can infect emerald tree boas, broadening the range of reptiles known to be infected by this primarily human pathogen.  相似文献   

Biochar Mitigates Salinity Stress in Potato          下载免费PDF全文

S. S. Akhtar  M. N. Andersen  F. Liu 《Journal of Agronomy and Crop Science》2015,201(5):368-378

A pot experiment was conducted in a climate‐controlled greenhouse to investigate the growth, physiology and yield of potato in response to salinity stress under biochar amendment. It was hypothesized that addition of biochar may improve plant growth and yield by mitigating the negative effect of salinity through its high sorption ability. From tuber bulking to harvesting, the plants were exposed to three saline irrigations, that is 0, 25 and 50 mm NaCl solutions, respectively, and two levels of biochar (0 % and 5 % W/W) treatments. An adsorption study was also conducted to study the Na⁺ adsorption capability of biochar. Results indicated that biochar was capable to ameliorate salinity stress by adsorbing Na⁺. Increasing salinity level resulted in significant reductions of shoot biomass, root length and volume, tuber yield, photosynthetic rate (A_n), stomatal conductance (g_s), midday leaf water potential, but increased abscisic acid (ABA) concentration in both leaf and xylem sap. At each salinity level, incorporation of biochar increased shoot biomass, root length and volume, tuber yield, A_n, g_s, midday leaf water potential, and decreased ABA concentration in the leaf and xylem sap as compared with the respective non‐biochar control. Decreased Na⁺, Na⁺/K⁺ ratio and increased K⁺ content in xylem with biochar amendment also indicated its ameliorative effects on potato plants in response to salinity stress. The results suggested that incorporation of biochar might be a promising approach for enhancing crop productivity in salt‐affected soils.  相似文献   

Genomewide association study reveals novel quantitative trait loci associated with resistance towards Septoria tritici blotch in North European winter wheat          下载免费PDF全文

Nana Vagndorf  Nanna Hellum Nielsen  Vahid Edriss  Jeppe Reitan Andersen  Jihad Orabi  Lise Nistrup Jørgensen  Ahmed Jahoor 《Plant Breeding》2017,136(4):474-482

Fungal diseases are a major constraint for wheat production. Effective disease resistance is essential for ensuring a high production quality and yield. One of the most severe fungal diseases of wheat is Septoria tritici blotch (STB), which influences wheat production across the world. In this study, genomewide association mapping was used to identify new chromosomal regions on the wheat genome conferring effective resistance towards STB. A winter wheat population of 164 North European varieties and breeding lines was genotyped with 15K single nucleotide polymorphism (SNP) wheat array. The varieties were evaluated for STB in field trials at three locations in Denmark and across 3 years. The association analysis revealed four quantitative trait loci, on chromosomes 1B, 2A, 5D and 7A, highly associated with STB resistance. By comparing varieties containing several quantitative trait loci (QTL) with varieties containing none of the found QTL, a significant difference was found in the mean disease score. This indicates that an effective resistance can be obtained by pyramiding several QTL.  相似文献   

Acute‐phase proteins as diagnostic markers in horses with colic          下载免费PDF全文

Tina H. Pihl DVM  PhD  Elrien Scheepers BVSc  MSc  Macarena Sanz DVM  MS  DACVIM  Amelia Goddard BVSc  MMed Vet  Nils Toft Cand Sci  PhD  Mads Kjelgaard‐Hansen DVM  PhD  Pia H. Andersen DVM  PhD  DVSc  Stine Jacobsen DVM  PhD 《Journal of Veterinary Emergency and Critical Care》2016,26(5):664-674

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Profits and nitrate leaching under irrigated corn: a simulation     

Gilbert D. Miller  R. J. Hanks  Jay C. Andersen  Glade R. Erikson 《Irrigation Science》1993,13(4):165-170

The economic impact of reducing the amount of nitrate leached out of the root zone under irrigation in the arid West was examined. The economic incentives of irrigation management were evaluated under the assumptions of both profit-maximizing and utility-maximizing (in reducing cost and effort expended in irrigation) decision-making criteria. The results indicate that there is a coincidence of interests of the farmer and the environment provided some leaching occurred. If no leaching is allowed, profit decreases markedly. Both behaviors result in less nitrate leaching than less profitable or less utility-producing irrigating practices.  相似文献   

Inhibition of juvenile hormone biosynthesis and methyl farnesoate epoxidase activity by 1,5-disubstituted imidazoles in the cockroach,Diploptera punctata     

Gopalan C. Unnithan  John F. Andersen  Tomomi Hisano  Eiichi Kuwano  Ren Feyereisen 《Pest management science》1995,43(1):13-19

Seventeen substituted imidazoles were tested as inhibitors of juvenile hormone (JH) III synthesis by cockroach corpora allata in an in-vitro radio-chemical assay. Most of these 1,5-disubstituted imidazoles were highly potent, with IC₅₀ values of less than 100nM. The compounds differed in their ability to cause an accumulation of the precursor methyl farnesoate in the glands. Four of the imidazoles were tested by topical application to previtellogenic adult females, and all caused a significant inhibition of JH synthesis and an accumulation of intraglandular methyl farnesoate for at least three days after treatment. Methyl farnesoate epoxidase activity of homogenates of corpora allata was inhibited by the compounds TH -14 and TH -27. This P450-dependent epoxidase activity was inhibited at less than 10 nM. The results show that the 1,5-disubstituted imidazoles are powerful inhibitors of the last step of juvenile synthesis in this cockroach.  相似文献