Cloning of plasma membrane H-ATPase gene in Populus euphratica Oliv.     

Ning De-juan Hou Pei-chen Hu Zan-min Shen Xin Chen Shao-liang College of Biological Sciences  Biotechnology  Beijing Forestry University  Beijing  P. R. China Institute of Genetics  Developmental Biology  Chinese Academy of Sciences  Beijing  P. R. China 《中国林学(英文版)》2006,8(4):15-19

For this paper, the plasma membrane (PM) H -ATPase gene has been cloned from Populus euphratica Oliv. through a homology based strategy. The isolated 3,210 bp cDNA contains a single 2,862 bp open reading frame (ORF) which encodes a putative H -ATPase protein of 953 amino acid residues, with a significant homology to plasma membrane H -ATPase of Primus persica, Phaseolus vulgaris, Sesbania rostrata and Daucus carota. The predicted protein has a molecular weight of 104,553 Da. The copy number analysis revealed multiple copies of the PM H -ATPase in the P. euphratica genome after digestion of their genomic DNA by the restriction enzymes EcoRI, NdeⅠ, FbaⅠand BglⅡ, and Southern blot.  相似文献   

EFFECT OF HIGH LOADS IN THE BRIDGE LOAD SPECTRUM ON FATIGUE CRACK GROWTH OF 16 Mn STEEL     

Zhong An  Shen Xianrong 《保鲜与加工》1995,(2):15-22

This paper studies an effect of high loads in Rayleigh distribution random load-ing on fatigue crack growth wi th 16 Mn steel compact tenion specimen,The results show that thehigh loads in the load spectrum close to service load of steel bridges accelerate crack growth and re-duce crack growth life,Lives predicted with constant amplitude da/dn data and equivalent loads arequite in agreement with test lives.  相似文献   

ELLIPSOMETRIC SPECTROSCOPY OF ELECTROCHROMISM OF POLYANILINE FILMS     

Xie Shangfen Shen Hui 《保鲜与加工》1991,(4):93-96

The electrochromism of polyaniline films, which is prepared by electropolymer-ization from aniline in acidic aqueous solution onto platinum,is investigated in 330-700nm through in-situ ellipsometric technique. The results indicate that the ellipsometric peak of the reduced state(-0. 2V) of the film appears at 390-400nm and the oxidized state(0. 55V) at 500-530nm. The elec-trochromic reaction from the reduced "yellow" state to the oxidized "green" state is suggested via intermediate state whose ellipsometric peak appears at 466-470nm.  相似文献   

Pharmacokinetics of florfenicol in healthy and Escherichia coli-infected broiler chickens     

Shen J  Wu X  Hu D  Jiang H 《Research in veterinary science》2002,72(2):137-140

The aims of this study were to evaluate the effects of a ketamine/propofol anaesthetic protocol in lions (Panthera leo), and to compare it to two commonly used anaesthetic protocols. Seventeen adult lions were anaesthetised using three different protocols. Group XK (n=6) was anaesthetised with intramuscular (i.m.) injections of xylazine and ketamine. Group KD (n=5) was anaesthetised with an i.m. injection of ketamine, followed by an intravenous (i.v.) injection of ketamine and diazepam. Group KP (n=6) was anaesthetised with an i.m. injection of ketamine followed by an i.v. injection of propofol. There was a significant difference in heart rate (P<0.0002), which was lowest in group XK and highest in KD. Jaw tone was significantly lower in Group XK (P<0.05). No undesirable effects were noted following injection of the propofol. Propofol was a suitable and safe drug for maintenance of anaesthesia in adult lions.  相似文献   

Interleukin-6 expression in gut of parasite challenged sheep     

Shen J  Bao S  McClure SJ  Emery DL  Husband AJ 《Veterinary immunology and immunopathology》2000,76(1-2):163-168

Following challenge with Trichosirongylus colubrifonizis, increased numbers of T-cells and immunoglobulin responses are seen in the intestine of sheep immunised by repeated infection with live worms. IL-6 mRNA expression in the small intestine from T. colubriformis-immunised and naive sheep was determined by in situ hybridisation, whereas CD4(+), IgA(+), IgG(+) cells in the gut were evaluated by immunohistochemistry. There was constitutive expression of IL-6 mRNA by cells in the naive gut, and the number of these cells was increased by parasite challenge. There were corresponding increases in numbers of CD4(+) and TCR gamma/delta(+) T-cells and IgG(+) B-cells. Our data are consistent with a role for IL-6, perhaps produced by CD4(+) and/or TCR gamma/delta(+) T-cells or B-cells, in B-cell terminal differentiation. Infiltration of B-cells, particularly IgG(+) B-cells, may reflect parasite immunity in the host.  相似文献   

Transcript expression profiles of <Emphasis Type="Italic">Takifugu rubripes</Emphasis> spermatozoa and eggs by expressed sequence tag analysis     

Shen XY  Cui JZ  Gong QL  Liu YJ  Nagahama Y 《Fish physiology and biochemistry》2008,34(3):235-243

  相似文献   

Construction of cDNA Library from Populus euphratica   总被引：1，自引：0，他引：1  

Yu Guangjun Wang Yiqin Shen Xin * College of Biological Sciences  Biotechnology  Beijing Forestry University  Beijing  P.R. China Genetics Institute  Chinese Academy of Sciences  Beijing  P.R. China 《中国林学(英文版)》2003,5(2):7-9

1 Introduction Populus euphratica is a salt tolerant tree species, which is mainly distributed in the desert regions in northwest China (Wei 1993). Moreover, it is the only large tree species, which can survive and develop into forest in these arid and saline-alkali areas. P. euphratica forest plays a very important role in restraining the expansion of desert, maintaining ecological environment in west China, protecting the biological diversity and raising the local people抯 living standard…  相似文献   

Suppressive effect of a proanthocyanidin-rich extract from longan (Dimocarpus longan Lour.) flowers on nitric oxide production in LPS-stimulated macrophage cells     

Ho SC  Hwang LS  Shen YJ  Lin CC 《Journal of agricultural and food chemistry》2007,55(26):10664-10670

Anthocyanidins found in certain flowers have been shown to act as strong antioxidants in various systems, exhibiting multiple biological actions. The antioxidative effects of water extract and ethanolic extract of longan (Dimocarpus longan Lour.) flowers were evaluated by radical scavenging activity and compared to those of gallic acid, myricetin, and epigallocatechin gallate. In this study, the suppressive effects of longan flower extracts on nitric oxide and prostaglandin E2 production were investigated using a lipopolysaccharide-stimulated RAW 264.7 cell model. Abundant levels of phenolic compounds including flavonoids, condensed tannins, and proanthocyanidins were found in water or ethanolic extracts prepared from dried longan flowers. The antioxidative effect of longan flower extract was similar to the effect exhibited by pure antioxidants. Moreover, longan flower extract showed prominent inhibitory effects on prostaglandin E2 production. Significant concentration-dependent inhibition of nitric oxide production was detected when cells were cotreated with lipopolysaccharide and various concentrations of longan flower extracts. These inhibitory effects were further attributed to suppression of inducible nitric oxide synthase protein expression and not to reduced enzymatic activity. These results suggest that longan flower crude extracts, especially ethanolic extract, have antioxidant and anti-inflammatory effects, and the probable mechanism involves inhibition of inflammation by proanthocyanidins. Preliminary observations suggest that longan flower extract, especially alcoholic extract, could be another potential source of natural dietary antioxidant and anti-inflammatory agent.  相似文献   

Complete Genome Sequencing and Analysis of Rehmannia Mosaic Virus Isolate from Shanxi Province     

Wang De-fu  Zhang Xi-mei  Guo Shang  Shen Shao-fei  Long Dan-dan  Li Ling-yu  Niu Yan-bing 《东北农业大学学报(英文版)》2021,28(3):58-65

Using double-stranded RNA(dsRNA) technology and sequence-independent amplification(SIA), the molecular identification on infected Rehmannia glutinosa in the field with mosaic symptoms was performed and the whole-genome of the Rehmannia mosaic virus(ReMV) Shanxi isolate(ReMV-SX) was sequenced. Sequencing analysis showed that the virus that infected Rehmannia glutinosa was Rehmannia mosaic virus(ReMV). The full-length of the obtained ReMV-SX sequence(GenBank accession no. JX575184) was 6 395 nt, containing four open reading frames(ORFs). The sequence homology analysis of the complete nucleotide sequence showed that ReMV-SX was 93.8%-97.0% homologous to ReMV in Tobamovirus subgroup I, while only 49.8%-58.9% homologous to the isolates in subgroups II and III of the same genus. Phylogenetic analysis showed that ReMV-SX and ReMV-Henan formed a separate branch and had the closest genetic relationship. The results laid the foundation for ongoing researches in the taxonomic status and evolution of ReMV and for further investigating the pathogenic mechanism of ReMV infecting Rehmannia glutinosa.  相似文献   

提高母猪生产效率:营养与健康     

陈建康  Sung Woo Kim  Alexandra C. Weaver  Yan Bin Shen  Yan Zhao 《国外畜牧学(猪与禽)》2014,(Z1):7-13

本综述主要研究能够满足猪胎儿生长发育、乳腺生长和泌乳需求的母猪营养需要和平衡。本文将讨论饲喂策略如何根据母猪的养分需要进行调节,以提高它们的生产效率和健康。本文所用的大部分研究数据是基于1996~2013年之间所进行的研究。母猪的营养需要受母猪所处的妊娠阶段和胎次的影响。母猪日粮中的抗氧化剂浓度需要进行重新评估,以防止在妊娠后期和哺乳期出现极端的过氧化应激。在饲喂母猪时,考虑妊娠母猪的饲喂阶段和哺乳母猪的胎次,能提高母猪的生产寿命和健康状况。使用精选的营养成分和添加剂,似乎能帮助母猪提高生产力和改善健康状况。  相似文献