Brucellosis in migratory sheep flock from Maharashtra,India     

Chhaya P. Sonekar  Satyajit Kale  Smita Bhoyar  Neha Paliwal  S. V. Shinde  S. P. Awandkar  Wiqar Khan  S. P. Chaudhari  N. V. Kurkure 《Tropical animal health and production》2018,50(1):91-96

Brucellosis is a zoonotic disease worldwide distributed and having the economic as well as public health importance. The prevalence of brucellosis among sheep flock having history of abortions was studied. A total of 229 samples comprising of 157 blood and 72 clinical samples (vaginal swabs) were collected from 157 animals. Clinical samples were processed for the isolation of Brucella melitensis. Serum samples (n = 157) were tested by Rose Bengal plate test (RBPT) and i-ELISA. A total of 68 (43.31%) and 104 (66.24%) samples were positive by RBPT and ELISA, respectively. Brucella isolates (n = 2) were recovered from clinical samples. Both isolates demonstrated amplification for bcsp 31 and IS711 genes. On AMOS PCR, both the isolates amplified at 731 bp, i.e., belongs to B. melitensis species. The incidence of B. melitensis in a migratory flock warns the thorough testing and culling of Brucella-infected sheep from the flock on a continuous basis; otherwise, such incidence will be routine and poor farmers will be at a loss.  相似文献   

Optimization of production of nucleopolyhedrovirus ofHelicoverpa armigera throughout larval stages     

V. Kalia  S. Chaudhari  G. T. Gujar 《Phytoparasitica》2001,29(1):23-28

Studies were carried out to optimize production of nucleopolyhedrovirus of American bollworm,Helicoverpa armigera (Hübner), by treating larvae individually with an inoculum dose that allowed maximal larval growth and also gave the highest occlusion bodies (OB) yield/larva. The maximum virus yield of 12.2x 10⁹ OB/larva was obtained when 6-day-old larvae were fed individually with a dose of 1 x 10³ OB. Topical spiracular treatment of larvae as old as 8 days with 10 μ of 2x 10⁷ OB ml^-1 gave the highest yield, of 15.2x 10⁹ OB from 13-day-old larvae, of 12.8x 10⁸ OB from prepupae and of 1.49x 10⁸ OB from pupae at the time of their death. These studies showed that dietary inoculum is the best route for 6-day-old larvae and topical spiracular treatment is the best for 8-day-old larvae.  相似文献   

A gross indignity to humans     

Kolhekar A 《Science (New York, N.Y.)》2003,302(5653):2070

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Effect of thyroid hormone depletion on monoamines and expression patterns of catfish GnRH in the air-breathing catfish, Clarias gariepinus     

K. V. Sathya Sai Kumar  I. Swapna  Aparna Dutta Gupta  K. C. Majumdar  B. Senthilkumaran 《Fish physiology and biochemistry》2005,31(2-3):189-192

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Response: brain "identifier sequence"     

Owens GP  Chaudhari N  Hahn WE 《Science (New York, N.Y.)》1986,234(4779):1006

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Hematopoietic cell regulation by Rac1 and Rac2 guanosine triphosphatases     

Gu Y  Filippi MD  Cancelas JA  Siefring JE  Williams EP  Jasti AC  Harris CE  Lee AW  Prabhakar R  Atkinson SJ  Kwiatkowski DJ  Williams DA 《Science (New York, N.Y.)》2003,302(5644):445-449

The Rho guanosine triphosphatases (GTPases) Rac1 and Rac2 are critical signaling regulators in mammalian cells. The deletion of both Rac1 and Rac2 murine alleles leads to a massive egress of hematopoietic stem/progenitor cells (HSC/Ps) into the blood from the marrow, whereas Rac1-/- but not Rac2-/- HSC/Ps fail to engraft in the bone marrow of irradiated recipient mice. In contrast, Rac2, but not Rac1, regulates superoxide production and directed migration in neutrophils, and in each cell type, the two GTPases play distinct roles in actin organization, cell survival, and proliferation. Thus, Rac1 and Rac2 regulate unique aspects of hematopoietic development and function.  相似文献   

The microstructure of high--critical current superconducting films     

Chaudhari P  Legoues FK  Segmüller A 《Science (New York, N.Y.)》1987,238(4825):342-344

The microstructure of superconducting films that have shown high-critical current densities has been studied. The films are shown to be epitaxial and contain twins and precipitates. The main difference between these films and low current carrying samples is the absence of grain boundaries. These boundaries are therefore identified as the cause of the lower critical current in ceramic samples.  相似文献   

Spatially Resolved Observation of Supercurrents Across Grain Boundaries in YBaCuO Films     

Mannhart J  Gross R  Hipler K  Huebener RP  Tsuei CC  Dimos D  Chaudhari P 《Science (New York, N.Y.)》1989,245(4920):839-841

Spatially resolved resistivity measurements of current transport across individual grain boundaries have been made on superconducting YBa(2)Cu(3)O(7). These experiments were done by low-temperature scanning electron microscopy with a resolution of 1 to 2 micrometers, and they show directly the limitation of the critical current density caused by grain boundaries in YBa(2)Cu(3)O(7). Furthermore, complex spatial patterns of the current transport across grain boundaries were observed. These patterns reflect self-excited resonances of the grain boundaries and are closely correlated to the unexplained "sub-gap structure" in the current-voltage characteristics of polycrystalline YBa(2)Cu(3)O(7).  相似文献   

DUBA: a deubiquitinase that regulates type I interferon production     

Kayagaki N  Phung Q  Chan S  Chaudhari R  Quan C  O'Rourke KM  Eby M  Pietras E  Cheng G  Bazan JF  Zhang Z  Arnott D  Dixit VM 《Science (New York, N.Y.)》2007,318(5856):1628-1632

Production of type I interferon (IFN-I) is a critical host defense triggered by pattern-recognition receptors (PRRs) of the innate immune system. Deubiquitinating enzyme A (DUBA), an ovarian tumor domain-containing deubiquitinating enzyme, was discovered in a small interfering RNA-based screen as a regulator of IFN-I production. Reduction of DUBA augmented the PRR-induced IFN-I response, whereas ectopic expression of DUBA had the converse effect. DUBA bound tumor necrosis factor receptor-associated factor 3 (TRAF3), an adaptor protein essential for the IFN-I response. TRAF3 is an E3 ubiquitin ligase that preferentially assembled lysine-63-linked polyubiquitin chains. DUBA selectively cleaved the lysine-63-linked polyubiquitin chains on TRAF3, resulting in its dissociation from the downstream signaling complex containing TANK-binding kinase 1. A discrete ubiquitin interaction motif within DUBA was required for efficient deubiquitination of TRAF3 and optimal suppression of IFN-I. Our data identify DUBA as a negative regulator of innate immune responses.  相似文献   

Ribotyping of Indian Isolates of <Emphasis Type="Italic">Pasteurella multocida</Emphasis> Based on 16S and 23S rRNA Genes     

Saxena MK  Kumar AA  Chaudhari P  Shivachandra SB  Singh VP  Sharma B 《Veterinary research communications》2005,29(6):527-535

The applicability of ribotyping based on 16S and 23S rRNA was evaluated for molecular epidemiological studies. Forty-eight isolates of Pasteurella multocida isolated from different hosts and geographical locations and one reference isolate were ribotyped. Only four ribotypes were found. All the isolates including reference isolate from wild carnivores had the same ribotype, though they had different serotypes. The isolate from a tiger had one band in addition to the bands present in the major ribotype. The isolates from lions represented two ribotypes; of these ribotypes, one (r2) had an additional band of 3.6 kbp, which was absent in all other ribotypes. The second ribotype (r4) from a lion had one band missing (6 kbp) that was present in the other ribotypes. These isolates were further typed using ERIC-PCR and REP-PCR. With ERIC-PCR and REP-PCR, higher D values of 0.83 and 0.89 were obtained. The current study revealed that ribotyping is not a very efficient typing tool for use in molecular epidemiology for differentiation of isolates.  相似文献