Analysis of the reactivity of anti-bovine CD8 monoclonal antibodies with cloned T cell lines and mouse L-cells transfected with bovine CD8     

N D MacHugh  A Bensaid  C J Howard  W C Davis  W I Morrison 《Veterinary immunology and immunopathology》1991,27(1-3):169-172

Mouse L-cells transfected with bovine CD8 and two Theileria parva-infected cloned T cell lines expressing bovine CD8 were used to screen the panel of ten monoclonal antibodies (mAbs) submitted to the workshop. Eight of the ten mAbs reacted with the transfectant and both the cloned T cell lines. However, two mAbs CC58 and BAT82A did not recognise the transfectant and only reacted with one of the T cell lines. Further biochemical studies indicated that the eight mAbs react with both homo- and heterodimeric forms of bovine CD8 whilst the two mAbs CC58 and BAT82A react with only heterodimeric forms. These data suggest that bovine DC8 is encoded by two genes as is the case in mouse and man.  相似文献   

Microorganisms associated with pneumonia in slaughter weight swine.   总被引：2，自引：1，他引：1       下载免费PDF全文

R B Morrison  C Pijoan  H D Hilley    V Rapp 《Canadian journal of veterinary research》1985,49(2):129-137

The lungs of 334 pigs were obtained from two slaughter plants in Minnesota and examined in detail. Macroscopic and microscopic evaluation, direct fluorescence for Mycoplasma hyopneumoniae and bacterial culture were done on all of them and a subsample of 50 were selected for virus culture. Mycoplasma hyopneumoniae, Pasteurella multocida and Haemophilus spp. were detected in 24.0%, 34.1% and 27.0% of the lungs, commonly in conjunction with each other. One isolate of Haemophilus pleuropneumoniae serotype 2 was detected and this represents the first report of its presence in the United States. No virus was detected in any of the lungs. Lungs with both M. hyopneumoniae and Pasteurella multocida had the greatest amount of macroscopic pneumonia (9.8% of the lung). Lungs with M. hyopneumoniae or P. multocida alone had 4.9% and 5.2% of the lung involved with pneumonia respectively. Lungs with Haemophilus sp. Taxon "minor group" had 3.8% of the lung involved which was not significantly different from lungs with none of these organisms being detected (1.6%). There was a positive correlation between the extent of M. hyopneumoniae infection, as scored by FAT and the amount of macroscopic pneumonia present (r = 0.46; P less than 0.001). Likewise, there was a positive correlation between the estimated concentration of P. multocida present, as scored by the relative number of colonies on blood agar and the amount of macroscopic pneumonia present (r = 0.60; P less than 0.001). Microscopically, the amount of lymphoreticular proliferation, polymorphonuclear cells and alveolar macrophages were evaluated.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

Mixed models applied to the study of variation of grower-finisher mortality and culling rates of a large swine production system          下载免费PDF全文

A.J. Larriestra  D.G. Maes  J. Deen    R.B. Morrison 《Canadian journal of veterinary research》2005,69(1):26-31

Large scale production systems for swine are frequently organized in a hierarchical structure. Consequently, important production parameters, such as mortality and culling, can be analyzed at different levels. The major aims of this study were to assess variance components (VC) of mortality and culling rates attributed to sites and to barns within a site, and subsequently to investigate the impact of average entry weight, days on feed (length of the production turn), and season on the magnitude of the VC. Then, data from a large farm with 3 sites were collected during 5 y. In total, 1720040 pigs distributed in 1502 all-in/all-out grower-finisher groups were included. Linear mixed models were fitted for mortality and culling rates. The barn was modeled as the residual component (barn-to-barn variations) with production turn and site nested within production turn as random intercept variance components. Barn-to-barn pig group variation was the largest VC for mortality (63.08%), when no predictors were included in the models. Predictors, such as pigs placed on quarters 2 and 3, low average entry weight, and shorter production turn length, were associated together with higher mortality. The explained proportion of variance due to these predictors was about 12.05% and the VC for barn, site, and production turn were 67.6%, 17.6%, and 14.8%, respectively. Barn-to-barn variation was also the largest VC for culling rate (46.2%), but the same predictor mentioned above explained only about 1.4% of the variation. The VC for barn, site, and production turn were 46.8%, 21.3%, and 31.8%, respectively. Since the variability among barns far exceeded the variability among sites, the barn should be used as experimental unit in studies with grower-finisher mortality, culling rate, or both, as outcome variables.  相似文献   

A multi-method approach to delineate and validate migratory corridors     

Monica L. Bond  Curtis M. Bradley  Christian Kiffner  Thomas A. Morrison  Derek E. Lee 《Landscape Ecology》2017,32(8):1705-1721

  相似文献   

Root architecture of sorghum genotypes differing in root angles under different water regimes     

Xi Liang  John E. Erickson  Wilfred Vermerris  Diane L. Rowland  Lynn E. Sollenberger  Maria L. Silveira 《Journal of Crop Improvement》2017,31(1):39-55

Plant root architecture offers the potential for increasing soil water accessibility, particularly under water-limited conditions. The objectives of this study were to evaluate the root architecture in two genotypes of sorghum (Sorghum bicolor (L.) Moench) differing in root angles and to assess the influence of different deficit irrigation regimes on root architecture. The response of two sorghum genotypes, ‘Early Hegari-Sart’ (EH; steep root angle) and ‘Bk7’ (shallow root angle) to four irrigation treatments was investigated in two replicated outdoor studies using large pots. The results indicated that EH possessed steeper brace and crown root angles, fewer brace roots, greater root biomass, and root length density than Bk7 at deeper soil depths (i.e., 15–30 and 30–45 cm) compared with a shallower depth (i.e., 0–15 cm). Across the soil profile, EH had greater root length density and length of roots of small diameter (<1 mm) than Bk7. Accordingly, EH showed more rapid soil-water capture than Bk7. Different levels of irrigation input greatly affected root architecture. Severe deficit irrigation (25% of full crop transpiration throughout the season) increased the angle and number of crown roots, root biomass, and root length density compared with 75 and 100% of full crop transpiration treatments. Consequently, root system architecture can be effectively manipulated through both genotypic selection and irrigation management to ensure optimal performance under different levels of soil available water.  相似文献   

Effect of Fermentation and Oil Incorporation on the Retention of E Vitamers During Breadmaking     

Oliver Buddrick  Oliver A. H. Jones  Paul D. Morrison  Darryl M. Small 《Cereal Chemistry》2015,92(3):327-331

All forms of baking and processing cause a loss of nutrients, including vitamin E, but little is known about these occurrences or if they could be avoided. The objective of this research was to study the incorporation of palm oil and the stability of vitamin E in palm oil during breadmaking. Wheat meal and rye breads were baked with and without the addition of 0, 2, 5, or 8% palm oil. The eight E group vitamers (tocopherols and tocotrienols) were extracted by using accelerated solvent extraction, freeze dried, and then analyzed with normal‐phase HPLC. Compared with the controls, the inclusion of palm oil was found to increase the quantity of all forms of vitamin E in the final baked products. It is concluded that palm oil is effective in increasing the vitamin E content of whole grain bread.  相似文献   

Effect of orally administered Lactobacillus casei on porcine reproductive and respiratory syndrome (PRRS) virus vaccination in pigs     

Kritas SK  Morrison RB 《Veterinary microbiology》2007,119(2-4):248-255

The purpose of this study was to determine whether intranasal/oral administration of probiotics can assist vaccination efficacy against an important swine pathogen, porcine reproductive and respiratory syndrome (PRRS) virus (PRRSV). A controlled challenge trial was performed employing: (a) pigs vaccinated against PRRS and treated with a Lactobacillus casei, (b) pigs vaccinated against PRRS only, (c) pigs treated with L. casei only, and (d) pigs neither vaccinated against PRRS nor treated with L. casei. All pigs were challenged intranasally with a wild PRRSV strain. There was no difference in clinical signs or rectal temperature among the four groups. However, pigs that received L. casei gained significantly more weight than pigs that did not. Vaccinated pigs did not gain more weight than nonvaccinated pigs. Vaccinated groups had significantly fewer viraemic pigs on days post-challenge 4, 11 and 17 than nonvaccinated groups of pigs. There was no effect of probiotic on prevalence or duration of viraemia. Among viraemic pigs, there was no significant difference in mean log base(10) titer of PRRS virus among groups. These results suggest that orally administered L. casei does not affect immune response in such a way as to affect PRRS viraemia or nasal shedding. However, it still appears to provide significant benefit when administered during vaccination as indicated by the higher bodyweight gain following PRRS virus infection.  相似文献   

Cholera toxin promotes the generation of semi-mature porcine monocyte-derived dendritic cells that are unable to stimulate T cells   总被引：2，自引：0，他引：2  

Bimczok D  Rau H  Wundrack N  Naumann M  Rothkötter HJ  McCullough K  Summerfield A 《Veterinary research》2007,38(4):597-612

Cholera toxin (Ctx) is a powerful mucosal adjuvant with potential applications for oral vaccination of swine. Dendritic cells (DC) play a key role in the decision between immunity and tolerance, and are likely target cells for mediating Ctx functions in vivo. Therefore, we examined the capacity of Ctx to enhance stimulatory activity of porcine monocyte-derived DC (MoDC). Ctx promoted the development of a semi-mature DC phenotype, with decreased levels of MHC class II and CD40, but increased CD80/86 expression. These changes were associated with activation of extracellular signal-regulated kinase (ERK), but not NFkappaB or c-Jun N-terminal kinase (JNK). Functionally, Ctx-priming greatly diminished T cell stimulatory capacity both in antigen-specific and superantigen-induced proliferation assays. The lower proliferation rate was not due to increased apoptosis of either DC or T cells. Ctx suppressed TNFalpha secretion by MoDC, but induced IL-10 production. The observed effects on T cell proliferation could only be partially mimicked by IL-10 alone. However, addition of recombinant TNFalpha to co-cultures of Ctx-primed MoDC and lymphocytes restored lymphocyte proliferation in a concentration-dependent manner. Ctx-primed DC were not actively tolerogenic, since they could not suppress proliferative T cell reactions induced by untreated DC.  相似文献   

Immediate and delayed movement of resistant and susceptible adults of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) after short exposures to phosphine     

William R Morrison III  Daniel Brabec  Alexander Bruce  Frank H Arthur  Christos G Athanassiou 《Pest management science》2023,79(6):2066-2074

  相似文献   

Retrospective evaluation of factors associated with emergency frequency and survival in equids presenting for emergency care (2019–2020): 3071 cases     

Jessica Bouton DVM  Langdon Fielding DVM  DACVECC  DACVSMR  Dustin Major DVM  DACVS  Emma Deane DVM  DACVECC  Jennifer Mayer VMD  DACVECC  Diane Rhodes DVM  DACVIM 《Journal of Veterinary Emergency and Critical Care》2023,33(5):598-605

  相似文献