Altered vitamin D metabolic system in follicular cysts of sows     

Malgorzata Grzesiak  Magdalena Socha  Anna Hrabia 《Reproduction in domestic animals》2021,56(1):193-196

This study aimed to examine 25OHD₃ concentration in the fluid of follicular and follicular lutein cysts of sows in comparison with preovulatory follicles as well as immunolocalize vitamin D metabolic enzymes (CYP27B1 and CYP24A1) and determine their protein abundances in the cyst wall. We have shown for the first time that 25OHD₃ level in the fluid of both cyst types was significantly lower than in preovulatory follicles. Furthermore, we have demonstrated CYP27B1 and CYP24A1 protein immunolocalization and abundance in follicular and follicular lutein cysts. The abundance of protein for both metabolic enzymes was decreased in ovarian cysts when compared to preovulatory follicles. We propose that altered VD metabolism in ovarian cyst might associate with their formation in sows.  相似文献   

Fatty acid profile as affected by fat depot and the sex category of Polish Holstein-Friesian × Limousin fattening cattle fed silage ad libitum     

Monika Sobczuk-Szul  Magdalena Mochol  Zenon Nogalski  Paulina Pogorzelska-Przybyłek 《Animal Science Journal》2021,92(1):e13516

This study was designed to compare the fatty acid profiles of four types of fat depots from bulls, steers, and heifers. All animals were Polish Holstein-Friesian and Limousin crossbreds fattened semi-intensively—silage at libitum and concentrate in the amount corresponding to 30% of their net energy requirements. The fatty acid profile in intramuscular, intermuscular, and external and internal fat was determined. The intramuscular fat of bulls was most abundant in total PUFAs and n-6 PUFAs, and functional fatty acids C 18:2, C 20:4, and C 22:5 in comparison with steers and heifers. Regardless of sex category, intramuscular and external fat were characterized by higher levels of UFAs and a more desirable MUFA/SFA ratio than the remaining fat types. It should also be noted that external fat was more abundant in CLA than other fat types, and that the highest CLA content was found in bull fat, compared with the remaining sex categories of cattle.  相似文献   

Leptin and leptin receptors during malaria infection in mice   总被引：2，自引：0，他引：2  

Pulido-Mendez M  De Sanctis J  Rodríguez-Acosta A 《Folia parasitologica》2002,49(4):249-251

Leptin, which is involved in a range of physiological processes, could be an important factor in the pathogenesis of malaria. We found that levels of leptin in serum and urine in Plasmodium berghei-infected mice increased progressively after infection, reaching a maximum value on day 6 post-infection. Serum values were approximately five-fold higher in infected mice than in non-infected controls. A similar relation was found for values of leptin in urine. Soluble leptin receptor levels also increased significantly in serum, more or less in line with the leptin increase. Our work represents the first report of visibly augmented leptin and soluble leptin receptor secretion in malarial infection.  相似文献   

Effect of an entomopathogenic nematode,Steinernema carpocapsae Weiser (Nematoda,Steinernematidae), on carabid beetles in field trials with annual legumes     

D. Ropek  Dr. Magdalena Jaworska 《Journal of pest science》1994,67(5):97-100

Two species of carabid beetle:Bembidion properans Steph. andPterostichus cupreus L. were the most often captured into pitfall traps in the spring period on plots with various cultivars of annual legumes. The greatest density ofP. cupreus population in the field with annual legumes was observed in May, in the feeding and eggs laying period by pea weevilSitona lineatus L. (Col. Curculionidae) — the main legumes pest. In June the most frequent carabid wasB. properans, the small beetles destroying the eggs or young larvae ofSitona lineatus. No effect of usingS. carpocapsae nematodes to infect nontarget carabid species was found in case different doses of invasive larvae on the soil surface into traps, although the mortality of captured ground beetles was high. The length of life of Carabidae collected from traps with nematodes were not significantly decreased in comparison with the control group.

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Zusammenfassung Zwei Carabiden-Arten,Bembidion properans Steph. undPterostichus cupreus L. waren die beiden häufigsten Arten, die im Frühjahr in Bodenfallen in verschiedenen Kulturen einjähriger Leguminosen gefangen wurden. Die größte Dichte vonP. cupreus wurde im Mai festgestellt während der Fraß- und Eilege-Periode des Haupt-Leguminosenschädlings, des ErbsenrüßlersSitona lineatus L. (Col., Curculionidae). Im Juni warB. properans der häufigste Laufkäfer, der die Eier und Junglarven vonSitona lineatus zur Beute nahm. Die Hinzugabe invasiver Larven des NematodenSteinernema carpocapsae in mit gefangenen Laufkäfern besetzten Bodenfallen erbrachte keine gesicherte Infektion, obgleich die Carbadiden-Dichte in den Gefäßen hoch war. Die Lebensdauer von Carabidae in mit Nematoden besetzten Gefäßen unterschied sich nicht von jener in Kontrollgefäßen.

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With 2 figures and 2 tables  相似文献   

Über den Befall einjähriger Leguminosen durch den Erbsenrüßler,Sitona lineatus L. (Col., Curculionidae)     

Magdalena Jaworska 《Journal of pest science》1992,65(4):70-72

In early spring period early field pea and other cultivars of pea were most attractive plants forS. lineatus adults in comparison with bean cv. In May, in egg laying period, bean cv appeared to be the most preferable. These field studies are the first step on further experiments on determining the effect of host plant on susceptibility of the weevils to infection by entomopathogens. *** DIRECT SUPPORT *** A0135136 00002  相似文献   

Tricyclic Guanidine Alkaloids from the Marine Sponge Acanthella cavernosa that Stabilize the Tumor Suppressor PDCD4     

Tanja Grkovic  Johanna S. Blees  Magdalena M. Bayer  Nancy H. Colburn  Cheryl L. Thomas  Curtis J. Henrich  Megan L. Peach  James B. McMahon  Tobias Schmid  Kirk R. Gustafson 《Marine drugs》2014,12(8):4593-4601

A cell-based high-throughput screen that assessed the cellular stability of a tumor suppressor protein PDCD4 (Programmed cell death 4) was used to identify a new guanidine-containing marine alkaloid mirabilin K (3), as well as the known compounds mirabilin G (1) and netamine M (2). The structures of these tricyclic guanidine alkaloids were established from extensive spectroscopic analyses. Compounds 1 and 2 inhibited cellular degradation of PDCD4 with EC₅₀ values of 1.8 μg/mL and 2.8 μg/mL, respectively. Mirabilin G (1) and netamine M (2) are the first marine natural products reported to stabilize PDCD4 under tumor promoting conditions.  相似文献   

The use of a mimic to detect polymerase chain reaction-inhibitory factors in feces examined for the presence of Lawsonia intracellularis.     

Magdalena Jacobson  Stina Englund  András Ballagi-Pordány 《Journal of veterinary diagnostic investigation》2003,15(3):268-273

Lawsonia intracellularis is an intracellular organism that causes proliferative enteritis in pigs. This bacterium is difficult to culture, and antemortem demonstration of the microbe is therefore often performed on fecal samples by polymerase chain reaction (PCR). Polymerase chain reaction is sensitive and specific, but inhibitory factors in feces might cause false-negative results. This article describes the construction and use of an internal standard, a mimic. The mimic is amplified by the same primers as those used for L. intracellularis DNA and thus could indicate false-negative results in clinical samples. The amplicon was clearly visible when as few as 10 mimic molecules were added per amplification reaction and when no inhibitors werepresent. When fecal samples were spiked with the mimic, the detection limit was 10(2) molecules per PCR. Sixty clinical samples, 20 from wild boars, 20 from growing pigs with diarrhea, and 20 from pigs without diarrhea, were prepared by a boiling procedure and subjected to PCR together with 10(3) mimic molecules. Nine samples were positive, of which 7 originated from pigs with diarrhea and 2 from pigs without diarrhea. In 14 samples from wild boars, in 8 samples from pigs without diarrhea, and in 3 samples from pigs with diarrhea, neither the mimic nor the target DNA was visible. This indicated the presence of inhibitors in these samples. It is concluded that the mimic can be used as an internal control in the diagnosis of L. intracellularis to indicate inhibition of PCR.  相似文献   

Analysis of duplication in the Spanish durum wheat collection maintained in the CRF-INIA on the basis of agro-morphological traits and gliadin proteins     

Magdalena Ruiz  Edurne Aguiriano 《Genetic Resources and Crop Evolution》2004,51(3):231-235

A total of 106 potential duplicate cases involved 277 accessions were detected on the basis of passport data in the durum wheat collection maintained in the CRF-INIA. Similarity between accessions was measured by agro-morphological traits. The 90% of the agro-morphological duplication were verified with gliadin proteins, allowing identification of similar material with greater refinement than agro-morphological data. However, the results indicated not to decide for rationalisation only on the basis of molecular data.  相似文献   

Multiple Ebola virus transmission events and rapid decline of central African wildlife     

Leroy EM  Rouquet P  Formenty P  Souquière S  Kilbourne A  Froment JM  Bermejo M  Smit S  Karesh W  Swanepoel R  Zaki SR  Rollin PE 《Science (New York, N.Y.)》2004,303(5656):387-390

Several human and animal Ebola outbreaks have occurred over the past 4 years in Gabon and the Republic of Congo. The human outbreaks consisted of multiple simultaneous epidemics caused by different viral strains, and each epidemic resulted from the handling of a distinct gorilla, chimpanzee, or duiker carcass. These animal populations declined markedly during human Ebola outbreaks, apparently as a result of Ebola infection. Recovered carcasses were infected by a variety of Ebola strains, suggesting that Ebola outbreaks in great apes result from multiple virus introductions from the natural host. Surveillance of animal mortality may help to predict and prevent human Ebola outbreaks.  相似文献   

Organophosphorus pesticide residues in Mexican commercial pasteurized milk   总被引：4，自引：0，他引：4  

Salas JH  González MM  Noa M  Pérez NA  Díaz G  Gutiérrez R  Zazueta H  Osuna I 《Journal of agricultural and food chemistry》2003,51(15):4468-4471

A study was conducted to measure residues of 13 organophosphorus (OP) pesticides, widely used as dairy cattle ectoparasiticides or in crops used for animal feed, in homogenized and pasteurized Mexican milk samples. Four different milk brands with high distribution were collected biweekly during a 12 month period (n = 96) in supermarkets. OP pesticide residues were measured by gas chromatography with a flame photometric detector. Approximately 39.6% of the samples contained detectable levels of OP pesticide residues. Eight samples contained residues exceeding established maximum residue limits (MRL), and the OP pesticides present in these samples were dichlorvos (five samples), phorate, chlorpyrifos, and chlorfenvinphos (one sample, respectively). Average residues of 13 OP pesticides measured were below established MRLs ranging between 0.0051 and 0.0203 ppm.  相似文献