Pulsed-field gel electrophoresis and distribution of the genes zag and fnz in isolates of Streptococcus equi     

Lindmark H  Jonsson P  Engvall E  Guss B 《Research in veterinary science》1999,66(2):93-99

Streptococcus equi subsp. equi and subsp. zooepidemicus are important pathogens of the equine respiratory tract. Isolates of both subspecies were examined by pulsed-field gel electrophoresis (PFGE). With the exception of eight isolates, a unique band pattern was displayed for each of the 48 subsp. zooepidemicus isolates tested. A method to distinguish isolates of the genetically very homogeneous subsp. equi has hitherto not been available, although several methods have been tested. By the use of PFGE, 50 isolates of subsp. equi could be divided into eleven groups, each with a unique pulsotype. In addition, the recently characterised genes encoding the cell-wall proteins ZAG and FNZ of S. equi subsp. zooepidemicus strain ZV were shown by Southern blots to be present in all 98 tested isolates, including the type strains of the two subspecies. Binding assays showed that the expression of the two genes clearly differentiate between the two subspecies.  相似文献   

Evaluation of tickGARD(PLUS), a novel vaccine against Boophilus microplus, in lactating Holstein-Friesian cows     

Jonsson NN  Matschoss AL  Pepper P  Green PE  Albrecht MS  Hungerford J  Ansell J 《Veterinary parasitology》2000,88(3-4):275-285

The effects of vaccination with the Bm 86 vaccine TickGARD(PLUS) against infestation with cattle tick (Boophilus microplus) and of holding cattle on a feedpad until 09:00 hours after the morning milking was tested on 40 mid lactation Holstein cattle using a factorial design. Vaccination resulted in a 56% reduction in tick numbers in the field over one generation, and a 72% reduction in laboratory measures of the reproductive efficiency of ticks. The liveweight gain of vaccinated cattle over 27 weeks was 18.6 kg higher than that of controls, and vaccinated cattle tended to have lower somatic cell count in milk (SCC). There were no other significant differences in measures of production. Cattle kept on the feedpad after the morning milking carried 26% more ticks than those returned immediately to their paddocks.  相似文献   

Diagnostic value of rectal temperature of African cattle of variable coat colour infected with trypanosomes and tick-borne infections   总被引：1，自引：0，他引：1  

J.W. Magona  J. Walubengo  W. Olaho-Mukani  N.N. Jonsson  M.C. Eisler   《Veterinary parasitology》2009,160(3-4):301-305

Diagnosis of major endemic bovine parasitic diseases in sub-Saharan Africa such as trypanosomosis, theileriosis, anaplasmosis, babesiosis and cowdriosis is increasingly relying on clinical diagnosis due to deterioration of veterinary services and laboratory facilities. Pyrexia is a common clinical feature of aforementioned diseases whose detection relies on measurement of rectal temperature. The research undertaken in this study was aimed at assessing the effects of diurnal changes and variable coat colour of indigenous Nkedi Zebu cattle on the diagnostic value of rectal temperature under tropical conditions. The results revealed that variation in rectal temperature was significantly influenced by time of day it was taken and by the coat colour of the Nkedi Zebu cattle (P < 0.001). Rectal temperature experienced diurnal changes: steadily rising to reach a peak at 17.00 h before declining. The mean rectal temperature of unhealthy cattle was significantly higher (P < 0.05) than that of the healthy ones only between 13.00 and 17.00 h of the day. During which period the proportion of unhealthy cattle having a rectal temperature of 39.4 °C or higher was significantly higher than that of healthy ones (P < 0.001). Regarding the variable coat colour of indigenous breeds, rectal temperature among cattle of different coat colours was significantly different (P < 0.05). In conclusion it is important to consider diurnal changes in rectal temperature and differences due to variable coat colour of indigenous African breeds when measuring rectal temperature for assessing pyrexia, during clinical diagnosis of bovine trypanosomosis and tick-borne diseases that are endemic in many countries in sub-Saharan Africa.  相似文献   

The end consumer’s choice of floorcovering in the Netherlands and the United Kingdom: a comparative pilot study of substitute competition     

Ragnar Jonsson 《Journal of Wood Science》2005,51(2):154-160

In order to understand the competitive situation of wood, it is essential to consider the end consumer of building materials. The knowledge of factors affecting the end consumers choice of building material for specific purposes, i.e., the mechanisms of substitute competition, is limited. Field studies of the British and Dutch floorcovering markets revealed that context, usage context as well as the general life situation, is of crucial importance in substitute competition. This contextual character severely limits the usefulness and adequacy of interviews with fixed-reply alternatives, as well as classical statistical methods of analysis. In this article, a qualitative approach to data gathering is combined with multivariate analysis. The results indicate that by using this methodology it is possible to determine which are the decisive predictors of material preferences, make cross-cultural comparisons, and apprehend the underlying motives or perspectives. The results further show that, unlike the other floorcovering materials studied, the determinant reasons for choosing wood appear to be exclusively nonfunctional in nature.  相似文献   

The vertical distribution of fine roots of five tree species and maize in Morogoro,Tanzania     

K. Jonsson  L. Fidjeland  J. A. Maghembe  P. Högberg 《Agroforestry Systems》1988,6(1-3):63-69

In order to assess the possibility of root competition in agroforestry, the vertical distribution of fine roots (< 2 mm in diameter) of five tree species in pure two-year-old stands was compared to that of mature maize.Cassia siamea, Eucalyptus tereticornis, Leucaena leucocephala andProsopis chilensis had a rooting pattern similar to that of maize, i.e. a slow decline in fine root mass from 0–100 cm soil depth.Eucalyptus camaldulensis had its roots evenly distributed down to 100 cm. On an average, the fine root biomass of the tree species was roughly twice as that of the maize. We conclude that the studied tree species are likely to compete with maize and other crops with a similar rooting pattern for nutrients and water.  相似文献   

A method for automatic collimator alignment in microdensity measurements     

K. Pernestål  B. Jonsson 《Wood Science and Technology》1996,30(2):91-97

Summary In microdensitometry, based on X-ray absorption technique and a direct, electronic detector technique, it is usually not possible to observe the collimator and its alignment to the annual ring under investigation during the measurement. In this paper a method for automatic alignment of the collimator to the ring is described. The operating technique is based on an optimization of the resolution of the instrument at every annual ring while the collimator is rotated to the best position. The procedure has been in use a number of years and has proven itself to be efficient and reliable. The theory of operation for the procedure is described and its software structure is shown in a block diagram. Advantages and limitations of the procedure are discussed.The funding of this project is made by Nordbanken. The density profile in Fig. 5 has been recorded by Mr. Bo Larsson and is published with his kind permission  相似文献   

Cooperation in private forestry of post-soviet system: Forest owners’ cooperatives in Lithuania     

Marius Lazdinis  Aidas Pivoriūnas  Imantas Lazdinis 《Small-Scale Forestry》2005,4(4):377-389

The current state of cooperation in private forestry in Lithuania is examined, with a focus on the analysis of objectives, organisational structure and the ways forest owners’ cooperatives operate. A postal survey has been used as a main research instrument, the questionnaire consisting of a series of multiplechoice close-ended questions. This paper provides insights into the state forest enterprises and other private companies operating in the private forestry sector, and places forest owners’ cooperatives in a broader context of the private forestry sector. A typical forest owner’s cooperative in Lithuania has up to 10 members and about 20 clients to whom services are provided. The leaders of cooperatives indicate that the optimal number of clients using their services should not exceed 40. The main stated objectives of cooperatives are the provision of services to their members under the most attractive conditions, uniting members, and earning a profit for the members. These activities of cooperatives revolve around timber harvesting and trade. It is concluded that the process of cooperation of private forest owners in Lithuania is rather slow, although positive development can be observed.  相似文献   

First record of autochthonous canine ehrlichiosis caused by Ehrlichia canis in Romania          下载免费PDF全文

Doru Morar  Gheorghe Dărăbuş  Mirela Imre  Marius Stelian Ilie  Kálmán Imre 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2015,44(2):200-204

This case study describes the first genetically confirmed and clinically manifested autochthonous Ehrlichia canis infection in a 9‐year‐old female mixed‐breed dog from Romania. Health screening of the dog included clinical examination, evaluation of stained peripheral blood smear and hematologic variables, as well as serologic testing and molecular analysis. Clinical signs included fever, apathy, dehydration, pale mucous membranes, and weakness. The microscopic examination of the blood smear and immunologic assays for Borrelia burgdorferi, Anaplasma phagocytophilum, and E canis antibodies, and for Dirofilaria immitis antigen yielded negative results. Hematologic abnormalities included moderate nonregenerative anemia, leucopenia with neutropenia, and moderate thrombocytopenia. The biochemical abnormalities identified were hypoalbuminemia, and mildly increased serum enzyme activities of AST and ALT. In addition, increased urea and creatinine levels associated with low urine specific gravity and proteinuria were also present. Nested PCR amplification of the partial E canis 16S rRNA gene demonstrated the presence of this rickettsial pathogen in the dog's blood, which subsequently was confirmed through sequencing based on the 100% homology with GenBank deposited E canis isolates. After specific treatment with doxycycline (10 mg/kg, orally, SID) for one month, the proteinuria, and hematologic and serum biochemical abnormalities with the exception of mild azotemia resolved. This report supports the geographical expansion of canine ehrlichiosis caused by E canis in nonendemic regions of Europe.  相似文献   

Correction to: Nordic forest management towards climate change mitigation: time dynamic temperature change impacts of wood product systems including substitution effects     

Schulte  Maximilian  Jonsson  Ragnar  Hammar  Torun  Stendahl  Johan  Hansson  Per-Anders 《European Journal of Forest Research》2022,141(5):865-866

European Journal of Forest Research -  相似文献   

Nordic forest management towards climate change mitigation: time dynamic temperature change impacts of wood product systems including substitution effects     

Schulte  Maximilian  Jonsson  Ragnar  Hammar  Torun  Stendahl  Johan  Hansson  Per-Anders 《European Journal of Forest Research》2022,141(5):845-863

European Journal of Forest Research - Climate change mitigation trade-offs between increasing harvests to exploit substitution effects versus accumulating forest carbon sequestration complicate...  相似文献