Effect of dietary Schizochytrium microalga oil and fish oil on plasma cholesterol level in rats          下载免费PDF全文

T. Komprda  O. Škultéty  S. Křížková  G. Zorníková  V. Rozíková  R. Krobot 《Journal of animal physiology and animal nutrition》2015,99(2):308-316

The purpose of the study was to test the hypothesis that the dietary oils with different content of n‐3 polyunsaturated fatty acids (PUFA) eicosapentaenoic acid (EPA) + docosahexaenoic acid (DHA) affect plasma lipid level in rats in a different degree. The diets with 6% of fish oil (FO) and Schizochytrium microalga oil (SchO; EPA+DHA content in the diets 9.5 + 12.3 and 2.6 + 29.5% of the sum of total fatty acids, respectively) were used; the diet with 6% of safflower oil (high content of n‐6 PUFA linoleic acid, 65.5%; EPA+DHA content 0.7 + 0.9%) was used as a control. The difference between FO and SchO was established only in the case of plasma triacylglycerol (TAG) level: plasma TAG of the FO‐fed rats did not differ from the control rats (p > 0.05), while SchO decreased (p < 0.05) plasma TAG to 46% of the control. On the other hand, FO and SchO decreased (p < 0.05) total plasma cholesterol (TC) in rats in the same extent, to 73% of the control. Regarding the underlying mechanisms for the TC decrease, both SchO and FO up‐regulated hepatic Insig‐1 gene (181 and 133% of the control; p < 0.05), which tended (p = 0.15 and p = 0.19 respectively) to decrease the amount of hepatic nSREBP‐2 protein (61 and 66% of the control). However, neither SchO nor FO influenced hepatic 3‐hydroxy‐3‐methyl‐glutaryl‐CoA reductase gene expression (p > 0.05); SchO (but not FO) increased (p < 0.05) low‐density lipoprotein receptor mRNA in the liver. It was concluded that the decrease of total plasma cholesterol might be caused by an increased cholesterol uptake from plasma into the cells (in the case of SchO), but also by other (in the present study not tested) mechanisms.  相似文献   

Destratification in deep fish culture reservoirs in Israel: Practice and modelling     

Ana Milstein  Myron Zoran  Hans Juergen Krambeck 《Aquaculture International》2001,9(1):3-15

Thermal stratification negatively affects water quality, and thus is a threatto fish culture in deep water ponds and reservoirs. To prevent and controlit, a device based on an underwater propeller that vertically transportsoxygen rich warm water from the epilimnion down to the anoxic and colderhypolimnion was assembled. The empirical results so far obtained in Israelidual purpose reservoirs for field crop irrigation and fish culture aresummarized herein, and a mathematical model of the device's effect onreservoir thermal structure is presented.  相似文献   

The effect of Lumbricus rubellus and Lumbricus terrestris on zinc distribution and availability in artificial soil columns     

Mathilde I. Zorn  Cornelis A. M. Van Gestel  Herman Eijsackers 《Biology and Fertility of Soils》2005,41(3):212-215

This study investigated the impact of epigeic and (epi)anecic earthworms on the distribution and availability of zinc in the soil profile. Experiments were carried out with Lumbricus rubellus and Lumbricus terrestris in perspex columns (Ø 10 cm), filled with 20 to 23 cm non-polluted soil [organic matter 2%, clay 2.9%, pH 6.4 (0.01 M CaCl₂)], that was covered by a 3- to 5-cm layer of aged zinc-spiked soil (500 mg Zn/kg dry soil) and another 2 cm non-polluted soil on top. After 80 days, columns were sacrificed and sampled in a depth profile. Earthworm casts, deposited on top of the soil, were collected. Each sample was analyzed for total and 0.01 M CaCl₂-exchangeable zinc concentrations. L. rubellus did not go deeper than 3 cm into the soil and therefore no effect on zinc distribution in the soil could be detected. For L. terrestris, total zinc concentrations in the non-polluted layers were slightly but significantly higher in columns with earthworms, and so were the CaCl₂-exchangeable zinc concentrations in the polluted layers of these columns. Casts of L. terrestris collected from the soil surface showed higher total zinc concentrations than those from non-polluted soil. Casts were mainly placed on top of the soil. This study showed that these epigeic and (epi)anecic species have only a slight effect on zinc availability, and that deep burrowing species, like L. terrestris, are able to transport polluted soil from deeper layers to the soil surface.  相似文献   

Comparative Evaluation on Nitrogen Saturation of Forest Catchments in Japan and Northeastern United States     

Nobuhito Ohte  Myron J. Mitchell  Hideaki Shibata  Naoko Tokuchi  Hiroto Toda  Goro Iwatsubo 《Water, air, and soil pollution》2001,130(1-4):649-654

To analyze the differences in the status and processes of nitrogen saturation in Japan and northeastern United States, we examined the hydrobiogeochemistry of nitrogen of forested watersheds in these regions. Two distinct differences were found between watersheds in Japan compared with those in US. 1) In Japanese watersheds, marked decreases of NO₃ ^? concentration in surface waters during the summer growing season were not found and NO₃ ^? concentrations sometimes increased especially in the summer at nitrogen saturated sites. This contrast with watersheds in US where decreases in NO₃ ^? concentration during the summer are commonly observed except those watersheds in advanced stages of nitrogen saturation. These differences in NO₃ ^? concentration relationships can be attributed to climatic differences, with Japan having high precipitation and high discharge during the summer, while in many regions of North America lowest discharges are found in the summer. The climatic regime in Japan leads to high rates of mineralization and the rapid transport of NO₃ ^? to streams in summer. 2) Japanese watersheds, even those with high NO₃ ^? concentrations in surface waters, show little evidence of acidification. This is in contrast to sites in US where increased NO₃ ^? concentrations, especially during episodic events, result in surface water acidification.  相似文献   

The effect of two endogeic earthworm species on zinc distribution and availability in artificial soil columns     

Mathilde I. Zorn  Cornelis A.M. Van Gestel 《Soil biology & biochemistry》2005,37(5):917-925

The objective of this study was to determine the impact of earthworm bioturbation on the distribution and availability of zinc in the soil profile.Experiments were carried out with Allolobophora chlorotica and Aporrectodea caliginosa in 24 perspex columns (∅ 10 cm), filled with 20-23 cm non-polluted soil (OM 2%, clay 2.9%, pH 0.01 M CaCl₂ 6.4), that was covered by a 3-5 cm layer of aged zinc spiked soil (500 mg Zn/kg dry soil) and another 2 cm non-polluted soil on top. After 80 and 175 days, columns were sacrificed and each cm from the top down to a depth of 15 cm was sampled. Earthworm casts, placed on top of the soil, were collected. Each sample was analyzed for total and CaCl₂-exchangeable zinc concentrations.Effects of earthworm bioturbation were most pronounced after 175 days. For A. chlorotica, total and CaCl₂-exchangeable zinc concentrations in the polluted layers were lower with than without earthworms. Total zinc concentrations in the non-polluted layers were higher in columns with earthworms. Casts of A. chlorotica collected on the soil surface showed slightly higher total zinc concentrations than non-polluted soil. Casts were found throughout the whole column. For A. caliginosa there were no differences in total zinc concentration between columns with and without earthworms. CaCl₂-exchangeable zinc concentrations in the polluted layers were lower for columns with earthworms. Casts were mainly placed on top of the soil and contained total zinc concentrations intermediate between those in non-polluted and polluted soil layers.This study shows that different endogeic earthworm species have different effects on zinc distribution and availability in soils. A. chlorotica transfers soil throughout the whole column, effectively mixing it, while A. caliginosa decreases metal availability and transfers polluted soil to the soil surface.  相似文献   

Input-Output Budgets of Inorganic Nitrogen for 24 Forest Watersheds in the Northeastern United States: A Review     

Campbell  John L.  Hornbeck  James W.  Mitchell  Myron J.  Adams  Mary Beth  Castro  Mark S.  Driscoll  Charles T.  Kahl  Jeffrey S.  Kochenderfer  James N.  Likens  Gene E.  Lynch  James A.  Murdoch  Peter S.  Nelson  Sarah J.  Shanley  James B. 《Water, air, and soil pollution》2004,151(1-4):373-396

Input-output budgets for dissolved inorganic nitrogen (DIN) are summarized for 24 small watersheds at 15 locations in the northeasternUnited States. The study watersheds are completely forested, free of recent physical disturbances, and span a geographical region bounded by West Virginia on the south and west, and Maine on the north and east. Total N budgets are not presented; however, fluxes of inorganic N in precipitation and streamwater dominate inputs and outputs of N at these watersheds. The range in inputs of DIN in wet-only precipitation from nearby National Atmospheric Deposition Program (NADP) sites was 2.7 to 8.1 kg N ha^-1 yr^-1 (mean = 6.4 kg N ha^-1 yr^-1; median = 7.0 kg N ha^-1 yr^-1). Outputs of DIN in streamwater ranged from 0.1 to 5.7 kg N ha^-1 yr^-1 (mean = 2.0 kg N ha^-1 yr^-1; median = 1.7 kg N ha^-1 yr^-1). Precipitation inputs of DIN exceeded outputs in streamwater at all watersheds, with net retention of DIN ranging from 1.2 to 7.3 kg N ha^-1 yr^-1 (mean = 4.4 kg N ha^-1 yr^-1; median = 4.6 kg N ha^-1 yr^-1). Outputs of DIN in streamwater were predominantly NO₃-N (mean = 89%; median = 94%). Wet deposition of DIN was not significantly related to DIN outputs in streamwater for these watersheds. Watershed characteristics such as hydrology, vegetation type, and land-use history affect DIN losses and may mask any relationship between inputs and outputs. Consequently, these factors need to be included in the development of indices and simulation models for predicting 'nitrogen saturation' and other ecological processes.  相似文献   

Species-specific earthworm population responses in relation to flooding dynamics in a Dutch floodplain soil   总被引：3，自引：0，他引：3  

Mathilde I. Zorn  Cornelis A.M. Van Gestel  Herman Eijsackers   《Pedobiologia》2005,49(3):189-198

Earthworms dominate the animal biomass in moist floodplain soils. They are known to survive long periods in aerated water, but little is known about earthworm population dynamics in floodplain systems with changing inundation frequencies. This study determined earthworm population dynamics in a floodplain system, in relation to frequency and duration of flooding events. From October 2000 to May 2003 earthworms were hand sorted in the ‘Afferdensche en Deestsche Waarden’, a floodplain on the south bank of the river Rhine, near Druten, The Netherlands. Earthworm numbers and biomasses per age class (adult, subadult, juvenile) were recorded. Numbers and biomasses tend to decrease during flooding. Lumbricus terrestris was found in high numbers (>10/m²) only at the end of a flooding period. Allolobophora chlorotica was hardly affected by flooding; their biomass remained stable during the year. Aporrectodea caliginosa showed fluctuating numbers and biomasses during the sampling period that did not correlate with flooding frequency. Numbers and biomasses of Lumbricus rubellus were strongly reduced at the end of each flooding event, but their population densities fully recovered until next flooding event. Earthworm populations in floodplains fluctuate in time, depending on the season and on the time, duration and frequency of flooding. Different earthworm species react differently towards these flooding dynamics.  相似文献   

Biological control of trichostrongyles in calves by the fungus Duddingtonia flagrans fed to animals under natural grazing conditions     

M. Larsen  P. Nansen  J. Wolstrup  J. Grnvold  S. A. Henriksen  A. Zorn 《Veterinary parasitology》1995,60(3-4):321-330

The present study was conducted in the 1993 grazing season with yearling calves exposed to a pasture with a natural mixed trichostrongyle larval infection. It was shown that daily feeding with the microfungus Duddingtonia flagrans during the first 2 months of the season led to a lowered herbage infectivity and a reduced acquisition of Ostertagia sp. and Cooperia sp. later in the season. In addition, the procedure delayed the onset of clinical disease. This was due to the nematode-destroying effects of the fungi in the dung excreted by the fungus-treated calves, as evidenced by results from a parallel in vitro assay on faecal larval cultures. The paper discusses future research needs before practical biological control can be implemented.  相似文献   

A perspective on management of Helicoverpa armigera: transgenic Bt cotton,IPM, and landscapes          下载免费PDF全文

Sharon Downes  Darren Kriticos  Hazel Parry  Cate Paull  Nancy Schellhorn  Myron P Zalucki 《Pest management science》2017,73(3):485-492

Helicoverpa armigera is a major pest of agriculture, horticulture and floriculture throughout the Old World and recently invaded parts of the New World. We overview of the evolution in thinking about the application of area‐wide approaches to assist with its control by the Australian Cotton Industry to highlight important lessons and future challenges to achieving the same in the New World. An over‐reliance of broad‐spectrum insecticides led to Helicoverpa spp. in Australian cotton rapidly became resistant to DDT, synthetic pyrethroids, organophosphates, carbamates and endosulfan. Voluntary strategies were developed to slow the development of insecticide resistance, which included rotating chemistries and basing spray decisions on thresholds. Despite adoption of these practices, insecticide resistance continued to develop until the introduction of genetically modified cotton provided a platform for augmenting Integrated Pest Management in the Australian cotton industry. Compliance with mandatory resistance management plans for Bt cotton necessitated a shift from pest control at the level of individual fields or farms towards a coordinated area‐wide landscape approach. Our take‐home message for control of H. armigera is that resistance management is essential in genetically modified crops and must be season long and area‐wide to be effective. © 2016 Society of Chemical Industry  相似文献   

Rice Germin-Like Proteins: Allelic Diversity and Relationships to Early Stress Responses   总被引：1，自引：0，他引：1  

Rebecca M. Davidson  Patricia M. Manosalva  Jacob Snelling  Myron Bruce  Hei Leung  Jan E. Leach 《Rice》2010,3(1):43-55

Germin-like protein (GLP) markers were associated with quantitative trait loci (QTL) for resistance to the rice blast pathogen, Magnaporthe oryzae in multiple rice (Oryza sativa) mapping populations. Twelve paralogous OsGLP gene family members are located within the physical QTL region on chromosome 8, and gene silencing studies suggest that they contribute collectively to the resistance phenotype. We compared sequence and expression profiles of OsGLP alleles in two resistant and two susceptible parental rice lines to find functional polymorphisms that correlated with the resistant phenotype. Based on coding and promoter sequences, the genes belong to two germin subfamily groups (GER3 and GER4). OsGLP members from both subfamilies were constitutively expressed and developmentally regulated in all cultivars. Transient induction above constitutive levels was observed for some OsGLPs, especially GER4 subfamily members, at early time points after M. oryzae infection and mechanical wounding. Varying 5′ regulatory regions and differential expression of some family members between resistant and susceptible cultivars corresponded with differential hydrogen peroxide (H₂O₂) accumulation after the same stimuli. OsGLP of both GER subfamilies localized to the plant cell wall. The protein location and early gene induction suggest that OsGLPs protect rice leaves at early stages of infection before fungal penetration and subsequent ingress. Our data suggest that regulation of OsGLP genes defines resistant versus susceptible phenotypes.  相似文献