Study of Resistance to Orobanche ramosa in Host (Oilseed Rape and Carrot) and Non-host (Maize) Plants     

Naïma Zehhar  Pascal Labrousse  Marie-Claire Arnaud  Christian Boulet  Driss Bouya  André Fer 《European journal of plant pathology / European Foundation for Plant Pathology》2003,109(1):75-82

Orobanche ramosa is a parasitic Angiosperm responsible for severe yield losses in several economical crops. It is a serious threat in oilseed rape in France and Morocco and is appearing in carrot crops in Morocco. In this study, several varieties of oilseed rape and carrot were screened in order to identify resistant cultivars and to characterize the resistance mechanisms involved. All the 15 oilseed rape varieties tested were susceptible. In carrot, the varieties 'Colmar à coeur rouge' and 'Nantaise demi-longue' were susceptible, whereas 'Palaiseau' and 'Buror' were resistant. In the susceptible 'Colmar à coeur rouge' carrot no defence reactions were found and the development of the parasite inhibited carrot tap root formation. In the resistant carrot varieties, the parasite germinated, became attached to the host root but became necrotic before emergence. In 'Buror' carrot, formation of a mechanical barrier was associated with the restriction to the cortex of the parasite. In maize cv. 'Vigni', a non-host of O. ramosa, thickening of xylem vessels, cell divisions in the central cylinder and formation of an encapsulation layer were observed in association with restricted development of Orobanche haustoria.  相似文献   

Production of ex vivo lipopolysaccharide-induced tumor necrosis factor-α, interleukin-1β, and interleukin-6 is suppressed by trans-resveratrol in a concentration-dependent manner          下载免费PDF全文

Jean-Francois Marier  Keguan Chen  Peter Prince  George Scott  Jerome R.E. del Castillo    Pascal Vachon 《Canadian journal of veterinary research》2005,69(2):151-154

Trans-resveratrol is a biologically active compound present in certain foods that has anti-inflammatory and anticancer properties. These beneficial effects are derived from both the immune system and cytokines. The purpose of this study was to determine the immunomodulatory effect of trans-resveratrol on the ex vivo production of inflammatory and anti-inflammatory cytokines stimulated by lipopolysaccharides (LPS). Trans-resveratrol (0, 0.01, 0.1, 1, and 10 microM) was added to blood samples from male Sprague-Dawley rats (n = 6) along with 100 U of LPS (Escherichia coli serotype, 055B5). The samples were then incubated for 4 h at 37 degrees C and centrifuged. Finally, concentrations of tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), and IL-6 in the plasma were analyzed using an enzyme-linked immunosorbent assay (ELISA). The production of inflammatory (TNF-alpha and IL-1beta) and anti-inflammatory (IL-6) cytokines was suppressed by trans-resveratrol in a concentration-dependent manner. These results support the hypothesis that the immunomodulatory effect of trans-resveratrol plays an important role in disease conditions that involve an overproduction of inflammatory cytokines.  相似文献   

Predominance of the papGII allele with high sequence homology to that of human isolates among avian pathogenic Escherichia coli (APEC)     

Vandemaele FJ  Mugasa JP  Vandekerchove D  Goddeeris BM 《Veterinary microbiology》2003,97(3-4):245-257

Avian pathogenic Escherichia coli (APEC) are often found in poultry and are responsible for a set of diseases, commonly referred to as avian colibacillosis. One of the important virulence factors is adhesion to different epithelial surfaces, which is mediated by pili. P pili are thought to play a role by means of their PapG adhesin, which occurs in three molecular variants: PapGI, PapGII and PapGIII. This study is the first to determine and analyse the distribution of the different papG alleles in APEC. Our results show a significant predominance of the papGII allele above all other alleles or allele combinations. No statistically significant associations could be found between papG allele distribution and the type of bird, organ of isolation and O serogroup. Finally, the papGII and papGIII sequences showed high homology with mammalian (including human) source papG sequences.  相似文献   

Early dexamethasone treatment after implantation of a sciatic-nerve cuff decreases the concentration of substance P in the lumbar spinal cord of rats with neuropathic pain          下载免费PDF全文

Francis Beaudry  Christiane Girard    Pascal Vachon 《Canadian journal of veterinary research》2007,71(2):90-97

The main purpose of this study was to evaluate the effects of early dexamethasone treatment on pain-related peptides at an early stage in the development of neuropathic pain induced by implantation of a sciatic nerve cuff in Sprague Dawley rats (body weight 250 to 350 g). The rats were tested for touch sensitivity with the use of von Frey filaments before and 3 d after cuff implantation (n = 12) or sham surgery (n = 6). Half of the cuff-implanted rats received dexamethasone, 1 mg/kg intraperitoneally, 1 h after surgery. Spinal cords were collected on the 3rd day after surgery, and the lumbar enlargement was processed for the detection of selected peptides (neurotensin, substance P, cholecystokinin [CCK], vasoactive intestinal peptide, and calcitonin gene-related peptide) by means of liquid chromatography and tandem mass spectrometry. The right sciatic nerve of each rat was collected, fixed, and stained for histopathological evaluation. Except for neurotensin, all the peptides showed an increased concentration with neuropathic pain; however, the differences were significant (P < 0.05) only for substance P and CCK. In the animals treated with dexamethasone, mechanical allodynia was less pronounced (P < 0.01), and only the concentration of substance P was decreased significantly (P < 0.05). Sciatic nerve sections showed a decrease in C (P < 0.01) and Adelta (P < 0.03) fibres with neuropathic pain and a nearly normal percentage of C fibres after dexamethasone treatment. The dexamethasone-treated animals also had less inflammation detectable microscopically at the nerve constriction site compared with cuff-implanted animals that were not treated with dexamethasone. Our results suggest that in the early stages of neuropathic pain induced by an inflammatory process, dexamethasone may be a useful treatment and that substance P plays an important role in pain perception.  相似文献   

Development and validation of a radioimmunoassay for thyrotropin in cattle.     

Hughes Guyot  José Sulon  Jean-Fran?ois Beckers  Jean Closset  Pascal Lebreton  Laurent Alves de Oliveira  Frédéric Rollin 《Journal of veterinary diagnostic investigation》2007,19(6):643-651

In mammals, thyrotropin, or thyroid-stimulating hormone (TSH), assay is used for the diagnosis of primary hypothyroidism. Hypothyroidism is the most common type of thyroid disorder in cattle. The aim of this study was to develop and validate, under physiologic and pathologic conditions, a radioimmunoassay (RIA) for bovine TSH (bTSH). Double RIA was performed with purified bTSH and specific bovine antiserum. Laboratory validation included research of minimal detection limit, accuracy, and reproducibility. The physiologic validation included a thyrotropin-releasing hormone (TRH) challenge performed on euthyroid cows and a follow-up of bTSH concentration over a 24-hour period. Furthermore, bTSH concentration was assayed in a large population of healthy dairy and beef cows to define reference interval. The pathologic validation was made by assaying bTSH and thyroid hormones on healthy and goitrous newborn calves. The minimum detection limit (MDL) for bTSH assay was 1.3 microU/ml. The recovery was 101% to 106%. The intra- and interassay coefficients of variation (CVs) ranged from 5% to 11% and 11% to 15%, respectively. The RIA covered the whole range of physiologic bTSH values, as shown by bTSH values induced by TRH-challenge. A pulsatile secretion of bTSH was observed, accompanied by a diurnal variation with lower night values than day values. Reference intervals of bTSH ranged from 1.3 to 13.0 microU/ml for beef and dairy breeds. Finally, bTSH easily discriminated goitrous newborn calves from healthy ones, leading to the definition of a cutoff value of 35 microU/ml. The bTSH assay positively reacted to physiologic and pathologic conditions. The accuracy and precision of the RIA were satisfying.  相似文献   

Landscape patterns and agriculture: modelling the long-term effects of human practices on Pinus sylvestris spatial dynamics (Causse Mejean, France)     

Paul Caplat  Jacques Lepart  Pascal Marty 《Landscape Ecology》2006,21(5):657-670

This paper focuses on understanding human impact on landscape. Both ecological and human practices are analysed as interacting processes. An agent-based model integrating biological and historical knowledge is used to analyse the pattern of Scots Pine encroachment in a French Mediterranean upland. In the STIPA model, pine trees are autonomous agents and a cellular automaton simulates land-use. We test the effects of shifting cultivation on tree establishment at the landscape scale. This allows us to understand how agropastoral practices patterned this area from the 17th to 19th century: simulations show the importance of shifting cultivation in limiting woodland progression. Fallow duration linked to environmental heterogeneity is a significant factor for explaining pine dynamics and landscape patterning at the scale of the study region. We put this result in perspective with current rangeland management policies that often consider grazing as the most relevant tool for open landscape maintenance. Our results also show the importance of taking into account time-scale effects when linking landscape patterns to agricultural systems.  相似文献   

Associations between use of electric cow-trainers and clinical diseases, reproductive performance and culling in Swedish dairy cattle     

Pascal A. Oltenacu  Jan Hultgren  Bo Algers 《Preventive veterinary medicine》1998,37(1-4):77-90

The aims of this follow-up study were: (a) to evaluate the role of ECT technology as a risk factor for several diseases; and (b) to determine if the effects of these diseases on cows' reproductive performance and as risk factors for culling are influenced by exposure to ECTs. Diseases considered were retained placenta, metritis, ketosis, cystic ovaries, silent heat, milk fever, clinical mastitis, and foot and leg problems. We used historical and contemporary controls (with control herds selected to match the experimental herds for size and location). Data consisted of 10264 Swedish Red and White (SRB) and 5461 Swedish Friesian (SLB) lactation records in 150 herds of which 33 used cow-trainers. Logistic regression was used to estimate the effects of parity and exposure to electric cow-trainers on the risks of diseases and the effects of diseases and exposure to electric cow-trainers on risk of culling. The least-squares procedure was used to estimate the effects of diseases on reproductive performance.

The dominant effects associated with use of electric cow-trainers were an increased risk for silent heat, clinical mastitis, ketosis and culling relative to cows in herds not using cow-trainers. Diseases had negative effects on reproductive performance and the effects were larger for cows in herds using cow-trainers. In herds using electric cow-trainers, the largest increase in the interval from first service to conception (58 days) was caused by the occurrences of silent heat, cystic ovaries and the combination of two or more diseases. Retained placenta, metritis, cystic ovaries, clinical mastitis and a combination of two or more diseases increased the risk of culling about two times relative to healthy primiparous cows with the increase being greater for cows in herds using cow-trainers. Silent heat did not increase risk of culling in control groups, but was the largest risk factor for culling in the exposed group. We concluded that exposure to electric cow-trainers increased the incidence risk of silent heat, clinical mastitis, and ketosis and changed silent heat from a neutral disease with respect to culling to a major risk factor. Finally, exposure to cow-trainers increased the general negative effect of diseases on the cows' reproductive performance and risk for culling.  相似文献   

Dielectric properties of copper-ethanolamine treated Chinese fir (Cunninghamia lanceolata Hook.)     

Cao Jin-zhen Yu Li-li You Xiao-di Kamdem D. Pascal 《中国林学(英文版)》2007,9(4):239-245

In order to clarify the interaction between copper and wood substances in wood treated with copper containing water-borne wood preservatives, the dielectric constant ε′ and dielectric loss factor ε″ of untreated wood and wood treated with four concentration levels of copper-ethanolamine (Cu-EA) solutions were determined within a temperature range from –100 to 40°C and a frequency range from 100 to 1 MHz. Three dielectric relaxation processes were observed in the ε″ spectrum; among them R-I is based on the reorientation of methylol groups in the amorphous region of wood cell walls and R-II is related to wood extractives. R-III appeared in Cu-EA treated wood, and its magnitude decreases with the concentration of Cu-EA solutions used in this experiment. This relaxation process was considered to be based on the reorientation of copper-ethanolamine-wood complexes in wood cell walls. At low copper retention, the hydrogen in the complex can form hydrogen bonding with adjacent hydroxyl groups, which results in a strong bonding state between copper and wood; at high copper retention, the numerous copper-ethanolamine complexes not only hinder them from forming hydrogen bonding with adjacent wood molecules due to steric hindrance, but also weaken the interaction between wood molecules themselves, which corresponds to reducing ε″ values of both R-I and R-III processes. The results explain the fact of in-creasing copper leaching in wood treated with high concentration copper-based water-borne preservatives.  相似文献   

Biological performance of a formulation containing water-dispersible copper naphthenate and sodium fluoride against decay fungi     

Donatien Pascal Kamdem  Nzokou Pascal  Doug Herman  Zuju Shu 《Wood material science & engineering》2020,15(1):30-36

ABSTRACT

Two formulations of wood preservative one containing water-dispersible copper naphthenate (CuN) alone and in combination with sodium fluoride (NaF) were used to treat southern pine sapwood blocks to determine the effect of the addition of sodium fluoride in the QNS on the protection against wood decay fungi using laboratory soil block tests following AWPA (American Wood protection Association) standard E10-16 [American Wood Protection Association AWPA-E10. (2016 American Wood Protection Association. (2016) Standard E10-16 Laboratory Method for Evaluating the Decay Resistance of Wood-Based Materials Against Pure Basidiomycete Cultures: Soil/Block Test. [Google Scholar]) Laboratory Method for Evaluating the Decay Resistance of Wood-based Materials Against Pure Basidiomycete Cultures: Soil/Block Test]. The standard consists in exposing treated blocks to pure monoculture of white rot (Irpex lacteus (FP-105915) and Trametes versicolor (R-105)) and brown rot fungi (Gloeophyllum trabeum (Madison 617) and Postia placenta (Madison 698) during a specific period. The weight loss (WL) in percentage of treated blocks was used as an indicator of the biological performance of treated blocks against basidiomycetes at specific retentions. Minimum WL was obtained with blocks treated with the formulation containing mixture of QNS and sodium fluoride with the retention of 0.29?kg/m³ Cu and 0.51?kg/m³ of fluorine in treated blocks. A copper retention of 1.65?kg/m³ from blocks treated with formulations containing only QNS was needed to obtain similar WL to blocks containing blocks Cu++ and F? (fluorine). The lower Cu retention is attributed to a combination of the presence of the fluorine with some toxic activities against bacteria and decay and the interaction of anions fluorine and ionic Cu (II) in the aqueous treating solutions to form more soluble biological toxic hydrate of copper fluoride ((H₂O Cu (II) F2) in treated blocks compared to copper complexes less biological available in water-dispersible CuN-treated bocks.  相似文献   

Surface energy of preservative-treated southern yellow pine (Pinus spp.) by contact angle measurement     

Jinzhen Cao  Pascal D. Kamdem 《Frontiers of Forestry in China》2007,2(1):99-103

In this study, the contact angles of four different reference liquids (including distilled water, diiodomethane, formadide and glycerol) formed on the surfaces of wood, treated with chromated copper arsenate (CCA) and two other emerging copper-based water-borne systems (commercial names: NW and NS) were measured with sessile drop method. Based on the contact angle data, the surface energy was obtained from the acid-base approach. The total surface energy consisted of Lifshiz-van der Waals parameter and acid-base parameter. Results showed that the NW and CCA treatments made the wood surface more hydrophobic while the NS treatment had the reverse effect on the wood surface mainly owing to the increased penetration of earlywood. By using three liquids, diiodomethane, formamide and distilled water, the total surface energy obtained for untreated earlywood, untreated latewood, CCA-treated earlywood, CCA-treated latewood, NW-treated earlywood, NW-treated latewood, NS-treated earlywood and NS-treated latewood were 43.1, 44.5, 43.4, 45.1, 49.4, 40.6, 46.0 and 40.9 mJ/m², respectively. The surface energy of CCA-treated wood was almost the same as untreated wood. After NW and NS treatments, the surface energy of both earlywood and latewood changed a little. However, the change was not so obvious as to draw any further conclusion concerning the influence of NW and NS treatments on the surface energy of wood. __________ Translated from Journal of Beijing Forestry University, 2006, 28(4): 1–4 [译自: 北京林业大学学报]  相似文献