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The effects of root-applied chalcone at 0.15 mmol L−1 on the growth and lignin biosynthesis in maize were investigated. The contents of 4-coumarate: CoA ligase (4CL, EC 6.2.1.12) substrates in maize shoots were increased more rapidly in the samples with chalcone application than in the control and the increase occurred at ≤ 3 h after the application (HAA). The lignin content was reduced by chalcone at ≤ 6 HAA. The shoot growth was suppressed by chalcone at ≤ 9 HAA. Consequently, the results suggest that chalcone suppressed maize growth by inhibiting monolignol biosynthesis. 相似文献
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Hideki OGISO Masashi FUJINAGA Hideki SAITO Toshiaki TAKEHARA Shigeru YAMANAKA 《Journal of General Plant Pathology》2002,68(4):292-299
One hundred and sixteen isolates of Fusarium oxysporum f. sp. lactucae obtained from 85 fields in three crisphead lettuce-producing areas in Nagano Prefecture, Japan were typed for races using
differential cultivars Patriot, Banchu Red Fire and Costa Rica No. 4. They were also grouped into vegetative compatibility
groups (VCGs) using complementation tests with nitrate non-utilizing (nit) mutants. Two California strains reported as F. oxysporum f. sp. lactucum, a type culture of F. oxysporum f. sp. lactucae, and 28 avirulent isolates of F. oxysporum obtained from crisphead lettuce were included for comparison. Among Nagano isolates, 66 isolates were identified as race 1,
and 50 as race 2. Race 1 strains derived from Shiojiri and Komoro cities and race 2 from Kawakami village and Komoro city.
All isolates of race 2 were biotin auxotrophs, and the race could be distinguished based on its requirement for biotin on
minimal nitrate agar medium (MM). Pathogenic isolates were classified into two VCGs and three heterokaryon self-incompatible
isolates. Strong correlations were found between race and VCG. All the race 1 strains were assigned to VCG 1 except self-incompatible
isolates, and all the race 2 strains to VCG 2. The 28 avirulent isolates of F. oxysporum were incompatible with VCG 1 and VCG 2. California strains was vegetatively compatible with VCG 1, and they were assigned
to race 1. Based on vegetative compatibility, these two races of F. oxysporum f. sp. lactucae may be genetically distinct, and F. oxysporum f. sp. lactucae race 1 is identical to F. oxysporum f. sp. lactucum.
Received 7 May 2002/ Accepted in revised form 6 September 2002 相似文献
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Hiroshi BANNAI Manabu NEMOTO Koji TSUJIMURA Takashi YAMANAKA Ken MAEDA Takashi KONDO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(2):309-311
To increase the sensitivity of an enzyme-linked immunosorbent assay (ELISA) for equine herpesvirus type 4
(EHV-4) that uses a 12-mer peptide of glycoprotein G (gG4-12-mer: MKNNPIYSEGSL) [4], we used a longer peptide consisting of a 24-mer repeat sequence (gG4-24-mer:
MKNNPIYSEGSLMLNVQHDDSIHT) as an antigen. Sera of horses experimentally infected with EHV-4 reacted much more
strongly to the gG4-24-mer peptide than to the gG4-12-mer peptide. We used peptide ELISAs to test paired sera
from horses naturally infected with EHV-4 (n=40). gG4-24-mer ELISA detected 37 positive samples (92.5%),
whereas gG4-12-mer ELISA detected only 28 (70.0%). gG4-24-mer ELISA was much more sensitive than gG4-12-mer
ELISA. 相似文献
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Ken‐ichi YAMANAKA Masahiro KANEDA Yasushi INABA Koji SAITO Kaiyu KUBOTA Miki SAKATANI Satoshi SUGIMURA Kei IMAI Shinya WATANABE Masashi TAKAHASHI 《Animal Science Journal》2011,82(4):523-530
Many observations have been made on cloned embryos and on adult clones by somatic cell nuclear transfer (SCNT), but it is still unclear whether the progeny of cloned animals is presenting normal epigenetic status. Here, in order to accumulate the information for evaluating the normality of cloned cattle, we analyzed the DNA methylation status on satellite I region in blastocysts obtained from cloned cattle. Embryos were produced by artificial insemination (AI) to non‐cloned or cloned dams using semen from non‐cloned or cloned sires. After 7 days of AI, embryos at blastocyst stage were collected by uterine flushing. The DNA methylation levels in embryos obtained by using semen and/or oocytes from cloned cattle were similar to those in in vivo embryos from non‐cloned cattle. In contrast, the DNA methylation levels in SCNT embryos were significantly higher (P < 0.01) than those in in vivo embryos from non‐cloned and cloned cattle, approximately similar to those in somatic cells used as donor cells. Thus, this study provides useful information that epigenetic status may be normal in the progeny of cloned cattle, suggesting the normality of germline cells in cloned cattle. 相似文献
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Satoshi SUGIMURA Ken-ichi YAMANAKA Manabu KAWAHARA Takuya WAKAI Masaki YOKOO Eimei SATO 《Animal Science Journal》2010,81(1):48-57
We investigated the effects of in vitro maturation duration and treatment with dibutyryl cyclic adenosine monophosphate (dbcAMP) on the blind enucleation efficiency and developmental competence of miniature pig somatic cell nuclear transfer (SCNT) embryos. Oocytes were cultured for 22 h in NCSU-23 medium with or without 1 mM dbcAMP and then additionally cultured in dbcAMP-free NCSU-23 for 14, 18, or 22 h. Regardless of dbcAMP treatment, the rate of nuclear maturation reached a plateau at 36 and 40 h. However, mitochondrial distribution, a marker for cytoplasmic maturation, differed between the dbcAMP-untreated oocytes at 36 h and dbcAMP-treated oocytes at 40 h. The metaphase II chromosomes were adjacent to the first polar body in 68.8% and 63.5% of the dbcAMP-untreated oocytes at 36 h and dbcAMP-treated oocytes at 40 h, respectively. Furthermore, the blind enucleation efficiency by removing a small volume of cytoplasm was significantly higher in the dbcAMP-untreated oocytes at 36 h (82.9%) and dbcAMP-treated oocytes at 40 h (89.9%) than other groups. The rate of blastocyst formation was highest in the dbcAMP-treated oocytes at 40 h. Hence, this study demonstrated that dbcAMP-treated early metaphase II oocytes are suitable for the production of miniature pig SCNT embryos. 相似文献
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本试验旨在研究饲粮中添加枯草芽孢杆菌(Bacillus subtilis,BS)对石门土鸡生长性能、屠宰性能、血清抗氧化指标和肠道形态的影响。选取540只55日龄、体重[(0.75±0.02) kg]相近的健康石门土鸡母鸡,随机分成3组,每组6个重复,每个重复30只鸡。对照组饲喂基础饲粮,试验组分别饲喂在基础饲粮的基础上添加300和600 mg/kg BS的试验饲粮。预试期7 d,正试期28 d。结果表明:1) 1～14 d时,与对照组相比,300、600 mg/kg BS组石门土鸡的平均日采食量(ADFI)和平均日增重(ADG)有提高趋势(P>0.05); 15～28 d和1～28 d时,各组间的ADFI、ADG和料重比(F/G)均无显著差异(P> 0.05)。2)与对照组相比,600 mg/kg BS组石门土鸡的屠宰率显著提高(P<0.05),300、600 mg/kg BS组的腹脂率有提高趋势(P>0.05);各组间的半净膛率、全净膛率、胸肌率、腿肌率均无显著差异(P>0.05)。3)与对照组相比,14 d时,300 mg/kg BS组石门土鸡的血清总超氧化物歧化酶(T-SOD)活性显著提高(P <0. 05); 14和28 d时,300、600 mg/kg BS组的血清谷胱甘肽过氧化物酶(GSH-Px)活性极显著提高(P <0.01)。4)与对照组相比,300、600 mg/kg BS组石门土鸡的十二指肠绒毛高度、绒毛宽度、隐窝深度均有提高趋势(P>0.05),300 mg/kg BS组的空肠绒毛宽度显著提高(P<0.05);各组间的回肠绒毛宽度、隐窝深度和绒隐比均无显著差异(P>0.05)。综上,饲粮中添加BS提高了石门土鸡的屠宰性能,增强了机体抗氧化能力,改善了肠道形态。 相似文献
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