Time-course comparison of pulmonary inflammation induced by intratracheal instillation of four different nickel oxide nanoparticles in male Fischer rats     

Toshio Kobayashi  Yutaka Oshima  Yasuhiro Tsubokura  Takako Muroi  Shozo Ajimi  Makoto Nakai  Kenji Kawaguchi  Takeshi Sasaki  Naohide Shinohara  Nobuya Imatanaka 《Journal of toxicologic pathology》2021,34(1):43

Occupational exposure to nickel oxide (NiO) is an important cause of respiratory tract cancer. Toxicity is known to be associated with the dissociated component, i.e. nickel (II) ions. To address the relationship between physicochemical properties, including solubility in artificial lysosomal fluid, of NiO and time-course changes in the pulmonary response, we conducted an intratracheal instillation study in male Fischer rats using four different well-characterized NiO products, US3352 (NiO A), NovaWireNi01 (NiO B), I small particle (NiO C), and 637130 (NiO D). The NiOs were suspended in purified water and instilled once intratracheally into male F344 rats (12 weeks old) at 0 (vehicle control), 0.67, 2, and 6 mg/kg body weight. The animals were euthanized on days 3, 28, or 91 after instillation, and blood analysis, bronchoalveolar lavage fluid (BALF) testing, and histopathological examination were performed. The most soluble product, NiO B, caused the most severe systemic toxicity, leading to a high mortality rate, but the response was transient and surviving animals recovered. The second-most-soluble material, NiO D, and the third, NiO A, caused evident pulmonary inflammation, and the responses persisted for at least 91 days with collagen proliferation. In contrast, NiO C induced barely detectable inflammation in the BALF examination, and no marked changes were noted on histopathology. These results indicate that the early phase toxic potential of NiO products, but not the persistence of pulmonary inflammation, is associated with their solubility.  相似文献   

The palliative efficacy of modified Mohs paste for controlling canine and feline malignant skin wounds     

Yasuhiro Fukuyama  Tetsushi Tezuka  Atsushi Kawabata  Takuya Maruo 《The Veterinary quarterly》2016,36(3):176-182

In veterinary medicine, the management of malignant skin wounds is highly challenging. We conducted a study on seven case animals (four dogs and three cats) which presented with malignant skin wounds. All seven animals had signs and symptoms which were controlled following treatment with a modified Mohs paste. Upon obtaining informed consent from their owners, the animals requiring management of malignant wounds were enrolled in this study. The modified Mohs paste was prepared by mixing zinc chloride, zinc oxide starch powder, glycerin, and distilled water. The modified Mohs paste was topically applied to and left to remain on the malignant wounds for one hour, under controlled conditions. Once the paste was removed, the wounds were irrigated with a solution of sterile saline. At the first examination, the wounds of each animal were observed for signs of exudate, malodor, and bleeding. In every case, visible improvement was observed immediately after the modified Mohs paste treatment. Specifically, the size of the malignant wounds, and the number of times the dressing gauze required changing, significantly decreased (p < 0.05 and p < 0.01, respectively). The open malignant skin wounds caused by mammary gland tumors disappeared in two cases. The Mohs paste has been shown to be a viable option for the palliative treatment in canine and feline malignant skin wound management.  相似文献   

Mutation of p53 Gene and Its Correlation with the Clinical Outcome in Dogs with Lymphoma          下载免费PDF全文

A. Koshino  Y. Goto‐Koshino  A. Setoguchi  K. Ohno  H. Tsujimoto 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2016,30(1):223-229

  相似文献   

Estrone sulfate and progesterone profiles during late gestation in recipient cows transferred embryos produced by nuclear transfer and in vitro fertilization     

Shah KD  Maeda T  Hidaka T  Ogata Y 《The Journal of reproduction and development》2007,53(6):1237-1246

The aim of present experiment was to evaluate the plasma concentrations of estrone sulfate (E(1)S) and progesterone (P(4)) during late gestation in recipient cows transferred embryos produced by nuclear transfer (NT) and in vitro fertilization (IVF). Blood samples were collected from recipients transferred embryos produced by NT (n=9) and IVF (n=13) at 160, 220, 240, 260 and 270 d of gestation and then at 5 d intervals until parturition. Plasma samples were analyzed for E(1)S and P(4) by ELISA. One NT and three IVF cows aborted between days 220 and 260 of gestation. Two NT and one IVF cow had prolonged gestation (over 290 d). One IVF cow had an overweight fetus (50 kg) after abortion (257 d). The patterns of changes in the concentrations of E(1)S during late gestation in the NT and IVF cows were almost identical. The NT and IVF cows that aborted had prolonged gestation and much higher E(1)S concentrations than the average. One NT cow aborted after 220 d of gestation and had a sudden high increase in its E(1)S concentration from 160 d to 220 d of gestation. The NT and IVF cows that had prolonged gestation also had significantly higher (P<0.05) P(4) concentrations than the average. These results raise the possibility that the E(1)S and P(4) profiles can be used to monitor some late gestational problems, such as higher birth weight, abortion and prolonged gestation.  相似文献   

Phosphorus and aluminium solubility relationships in acidic lowbush blueberry barren soils in Maine     

T. Ohno  N. J. Severy 《Soil Use and Management》2013,29(4):485-493

Lowbush blueberry (Vaccinium angustifolium) is a native plant that is not cultivated, but managed in areas of sufficient plant density to provide commercial yields. A cropping systems study was initiated to compare how organic and three levels of conventional (low, medium and high input) management practices affected soil properties at 12 grower fields in the lowbush blueberry barrens of Maine. The fields under organic and low‐conventional treatments did not receive any fertilizer inputs. The high and medium conventional treatment fields received optimal and reduced diammonium phosphate inputs, respectively. Three measurements of soil P (modified‐Morgan soil test, oxalate extractable and total P) showed no significant effect of management treatment on the phosphorus status of the soils. This suggests that soil P may be leaching below the 0–10 cm rooting zone which was investigated in this study. Equilibrium chemical speciation of soil/water extracts showed that gibbsite was controlling the solubility of Al in these barren soils and that P was undersaturated with respect to amorphous Al(OH)₂PO₄. A laboratory one‐point P sorption study showed that dissolved organic matter derived from the organic pad sampled from the study sites did not inhibit the adsorption of the added P. This suggests that addition of carbon‐rich soil amendments such as compost may not increase P bioavailability of these acidic soils with high Al (oxy)hydroxide (gibbsite) mineral content.  相似文献   

Synthesis,insecticidal activity and pyrethroidal mode of action of new tin ether derivatives     

Kazunori Tsushima  Toshihiko Yano  Kimitoshi Umeda  Noritada Matsuo  Masachika Hirano  Nobuo Ohno 《Pest management science》1989,25(1):17-23

A novel range of trimethylstanniomethyl ethers of well known pyrethroid alcohols were synthesised, and their insecticidal activities and modes of action as insecticides were investigated. Among them, ethers from three types of alcohol (3-phenoxybenzyl, 4-fluoro-3-phenoxybenzyl and 6-phenoxy-2-pyridylmethyl) showed remarkable insecticidal activities against rice stem borers, houseflies and German cockroaches. According to electrophysiological studies on the abdominal nerve cords of German cockroaches, trimethylstanniomethyl 6-phenoxy-2-pyridylmethyl ether induced a rapid decline in spontaneous firing similar to that from tetramethrin. However, insecticidal trimethyltin chloride caused an entirely different response. These observations suggest that the present tin ether derivatives resemble pyrethroids, rather than the insecticidal tin compounds known so far.  相似文献   

Telomere length and telomerase activity in canine mammary gland tumors     

Yazawa M  Okuda M  Setoguchi A  Iwabuchi S  Nishimura R  Sasaki N  Masuda K  Ohno K  Tsujimoto H 《American journal of veterinary research》2001,62(10):1539-1543

OBJECTIVE: To measure telomere length and telomerase activity in naturally occurring canine mammary gland tumors. SAMPLE POPULATION: 27 mammary gland tumor specimens obtained during resection or necropsy and 12 mammary gland tissue specimens obtained from healthy (control) dogs. PROCEDURE: Telomere length in tissue specimens was measured by use of restriction endonuclease digestion and Southern blot analysis. Telomerase activity was measured by use of a telomeric repeat amplification protocol assay. RESULTS: Telomere length in mammary gland tumors ranged from 11.0 to 21.6 kilobase pairs (kbp; mean +/- SEM, 14.5+/-0.5 kbp) but did not differ among tumor types. Telomeres in mammary gland tumors were slightly shorter than in normal tissue specimens, but telomere length could not be directly compared between groups, because mean age of dogs was significantly different between groups. Age was negatively correlated with telomere length in control dogs but was not significantly correlated with length in affected dogs. Telomerase activity was detected in 26 of 27 mammary gland tumors and in 4 of 12 normal tissue specimens. However, telomerase activity and telomere length were not correlated in tumor specimens. CONCLUSIONS AND CLINICAL RELEVANCE: Telomere length is maintained in canine mammary gland tumors regardless of the age of the affected dog. Measurement of telomere length may be a useful tool for monitoring the in vivo effects of telomerase inhibitors in dogs with tumors.  相似文献   

Molecular cloning of canine thymus and activation-regulated chemokine (TARC) gene and its expression in various tissues     

Maeda S  Mizuno T  Yamashita K  Kurata K  Masuda K  Ohno K  Tsujimoto H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(9):1035-1038

Thymus and activation-regulated chemokine (TARC) is known as a functional ligand for CC chemokine receptor 4 (CCR4), which is selectively expressed on Th2 lymphocytes and induces selective migration of the cells to allergic lesions. In this study, we cloned canine TARC cDNA from canine thymus by RT-PCR with rapid amplification of cDNA ends (RACE) method. The canine TARC clone contained a full-length open reading frame encoding 99 amino acids and included four cysteine residues characteristic to CC chemokine family. The canine TARC cDNA showed 77.5%, 67.4%, and 68.5% amino acid sequence similarity with human, mouse and rat homologues, respectively. Expression of TARC mRNA was detected not only in thymus but also in spleen, lymph node, lung and heart of the various normal dog tissues examined. TARC cDNA clone obtained in this study will be useful for further investigation on allergic diseases in dogs.  相似文献   

Pneumocystis carinii pneumonia in a Cavalier King Charles Spaniel     

Hagiwara Y  Fujiwara S  Takai H  Ohno K  Masuda K  Furuta T  Nakayama H  Doi K  Tsujimoto H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(3):349-351

Pneumocystis carinii pneumonia was diagnosed by postmortem examination of a one-year-old Cavalier King Charles Spaniel with four-week history of dyspnea. Cytologic and histologic examination of lung tissues revealed numerous P. carinii trophozoites and cysts, and P. carinii specific DNA was detected by polymerase chain reaction. The dog showed hypogammagloblinemia and extremely low levels of serum IgG. It was considered that P. carinii pneumonia in this case was associated with an immunodeficient condition which has already been reported in Miniature Dachshunds.  相似文献   

Invasion and colonization of mature apple fruit by Erwinia amylovora tagged with bioluminescence genes     

Koji Azegami  Takanori Tsukamoto  Takayuki Matsuura  Tatsuji Ohara  Yasuhiro Inoue  Akifumi Mizuno  Kouji Yoshida  Hideo Bessho  Shigeru Kimura  Masao Goto 《Journal of General Plant Pathology》2005,71(1):98-98

  相似文献