Postovulatory Effect of Intravenous Administration of Lipopolysaccharide (Escherichia coli, O55:B5) on the Endocrine Status of Recently Ovulated Sows     

AM Mwanza  A Madej  H Kindahl  N Lundeheim  & S Einarsson 《Reproduction in domestic animals》2001,36(3-4):157-161

The effects of lipopolysaccharide ( Escherichia coli , O55:B5), administered 18 h after ovulation in the second oestrus after weaning on the hormonal profiles in 14 Swedish cross-bred (Landrace × Yorkshire) multiparous sows were studied. The endotoxin group (E-group) sows were administered with 300 ng/kg of lipopolysaccharide (LPS) whereas the control group (C-group) sows were administered 5 ml of saline intravenously via an indwelling jugular cannula. Blood samples for hormonal analyses were collected from all sows until slaughter. In the E-group, progesterone, cortisol and prostaglandin F_2α metabolite levels increased significantly (p < 0.05) following LPS compared with the C-group. It can be concluded from this study that apart from elevating cortisol and prostaglandin F_2α metabolite, LPS also elevates progesterone levels.  相似文献   

Regulation of Capacitation of Canine Spermatozoa during Co-culture with Heterologous Oviductal Epithelial Cells   总被引：2，自引：0，他引：2  

AM Petrunkina  K Simon  A-R Günzel-Apel  E Töpfer-Petersen 《Reproduction in domestic animals》2003,38(6):455-463

Progress of essential steps of the capacitation is coordinated in the oviductal isthmus, where sperm are stored in close contact with the epithelium. A crucial capacitational event is the phosphorylation of sperm membrane proteins. Regulation of the tyrosine phosphorylation by the oviduct has not been examined in dog sperm yet. The aim of this work was to study the effect of dog sperm binding to porcine oviductal epithelium on capacitation‐induced cellular and molecular changes. Epithelial cells were stripped from the oviducts of post‐puberal sows and cultured for 5–7 days at 39°C and 5% CO₂ on Biomatrix‐covered Chamber slides. Sperm washed through Percoll was co‐incubated with the oviductal epithelium cell cultures in a bicarbonate Tyrode's medium. During co‐incubation, sperm membrane changes, the state of tyrosine phosphorylation and motility were determined after 3, 30, 90, 180, 240 and 360 min. Significant increases in the percentage of capacitated and dead cells were observed in unbound sperm, while bound sperm remained uncapacitated, live and motile. An increasing tyrosine phosphorylation of tail proteins in bound, unbound and control sperm suspensions and a subsequent phosphorylation of head proteins in unbound and control sperm suspensions were observed. A significant difference regarding head phosphorylation (p < 0.05) was found between sperm bound to oviductal epithelium and unbound sperm. Binding occurred mainly in sperm with non‐ phosphorylated heads, while higher proportions of phosphorylated cells were found in unbound populations. The head phosphorylation progressed significantly during incubation in unbound spermatozoa (p < 0.05); however, it was suppressed in population of sperm attached to oviductal epithelium. Significant correlations between motility parameters related to hyperactivation and tail phosphorylation were found in unbound sperm. These observations support the hypothesis that spermatozoa with non‐phosphorylated heads preferentially attach to epithelial cells. It can be concluded that tyrosine phosphorylation of head membrane proteins and capacitation are delayed in canine spermatozoa being in closed contact with oviductal epithelium.  相似文献   

Dr. Mark Francis: the man and the legend     

Norton R 《Veterinary heritage : bulletin of the American Veterinary History Society》1989,12(1):10-16

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Apoplastic redox metabolism: Synergistic phenolic oxidation and a novel oxidative burst     

C. Jacyn Baker  Daniel P. Roberts  Norton M. Mock  Bruce D. Whitaker  Kenneth L. Deahl  Andrey A. Aver&#x;yanov 《Physiological and Molecular Plant Pathology》2005,67(6):1322

The plant apoplast is an important mediator of communication between the cell cytoplasm and its surroundings. Plant cell suspensions offer a convenient model system to gain insight into apoplastic physiology. Here, we describe a novel phenomenon that took place when two naturally occurring phenolics were added together to either soybean or tobacco cell suspensions. Acetosyringone (AS) and/or hydroxyacetophenone (HAP), phenolics found in the extracellular/apoplast of tobacco cells, were added to soybean or tobacco cell suspensions undergoing an oxidative burst. Individually, AS appeared to be utilized as a typical peroxidase substrate to scavenge hydrogen peroxide, while HAP was utilized at a much lower rate. However, when added together the rate of utilization of both phenolics increased and surprisingly resulted in the production of hydrogen peroxide. We have further characterized this novel phenomenon in suspension cells. This study demonstrates that certain phenolics in plants can cause co-oxidation which, as in animals, could alter the structure and bioactivity of surrounding phenolics.  相似文献   

Letters & Perspectives     

RR Pascoe AM 《Australian veterinary journal》2005,83(3):133-133

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In situ provision of drinking water to grazing dairy cows improves milk production     

MM Miglierina  N Bonadeo  AM Ornstein  D Becú-Villalobos 《New Zealand veterinary journal》2018,66(1):37-40

AIMS: To determine the effect of providing water within the area grazed by dairy cows on milk yield and quality, compared to requiring cows to walk to a distant water trough, on a dairy farm in the Pampa region of Argentina during summer.

METHODS: Holstein dairy cows were allocated to two herds with similar parity, days in milk and milk production. They were grazed in one paddock that was divided in two, with a fixed water trough at one end. Cows were moved twice daily to grazing plots within the paddock. Control cows (n=66) could only access water from the fixed trough, whereas supplemented cows (n=67) also received water from a mobile trough within the grazing plot. Milk production of each cow, and water consumption of the two herds were measured daily over 62 days. Milk composition for each herd was determined weekly from Days 18 to 60 of the study, and grazing behaviour was observed between 08:00 and 16:00 hours on Days 11–15, 19–22 and 39–43.

RESULTS: Over the 62 days of the study, supplemented cows produced 1.39 (SE 0.11) L/cow/day more milk than Control cows (p=0.027). Estimated mean daily water intake was 50.4 (SE 2.1) L/cow/day for supplemented cows and 58.2 (SE 2.7) L/cow/day for Control cows (p=0.004). Percentage total solids in milk was higher for supplemented (12.5 (SE 0.06)%) than Control (12.4 (SE 0.04)%) cows (p=0.047). During the periods of behavioural observation, a higher percentage of cows in the water supplemented than the Control herd were observed in the grazing area (p=0.012).

CONCLUSIONS AND CLINICAL RELEVANCE: This preliminary study demonstrated that provision of water to dairy cows within the grazing plot was beneficial for milk production and composition, and may be associated with longer periods spent within the grazing area, during hot weather in the Pampa region of Argentina.  相似文献   

Can additional N fertiliser ameliorate the elevated CO2‐induced depression in grain and tissue N concentrations of wheat on a high soil N background?          下载免费PDF全文

M. Tausz  R. M. Norton  S. Tausz‐Posch  M. Löw  S. Seneweera  G. O'Leary  R. Armstrong  G. J. Fitzgerald 《Journal of Agronomy and Crop Science》2017,203(6):574-583

Elevated CO₂ stimulates crop yields but leads to lower tissue and grain nitrogen concentrations [N], raising concerns about grain quality in cereals. To test whether N fertiliser application above optimum growth requirements can alleviate the decline in tissue [N], wheat was grown in a Free Air CO₂ Enrichment facility in a low‐rainfall cropping system on high soil N. Crops were grown with and without addition of 50–60 kg N/ha in 12 growing environments created by supplemental irrigation and two sowing dates over 3 years. Elevated CO₂ increased yield and biomass (on average by 25%) and decreased biomass [N] (3%–9%) and grain [N] (5%). Nitrogen uptake was greater (20%) in crops grown under elevated CO₂. Additional N supply had no effect on yield and biomass, confirming high soil N. Small increases in [N] with N addition were insufficient to offset declines in grain [N] under elevated CO₂. Instead, N application increased the [N] in straw and decreased N harvest index. The results suggest that conventional addition of N does not mitigate grain [N] depression under elevated CO₂, and lend support to hypotheses that link decreases in crop [N] with biochemical limitations rather than N supply.  相似文献   

Performance,milk fatty acid composition and behaviour of high-yielding Holstein dairy cows given a limited grazing period     

Norton E. Atkins  Claire Cianchi  S. M. Rutter  Sarah J. Williams  Carrie Gauld  Gemma L. Charlton  Liam A. Sinclair 《Grass and Forage Science》2020,75(2):181-191

The effects of a limited grazing period on the performance, behaviour and milk composition of high-yielding dairy cows were examined. A total of 56 Holstein cows yielding 44.7 ± 0.42 kg/day were allocated to one of four treatments in one of two, 4-week periods. Treatments were as follows: control (C)—cows housed and offered TMR ad libitum; early grazing (EG)—cows grazed for 6 hr after morning milking then housed; delayed grazing (DG)—cows returned to housing for 1 hr after morning milking followed by grazing for 6 hr, then housed; restricted TMR (RT)—cows grazed for 6 hr after morning milking, then housed and fed TMR at 75% of ad libitum. Intake of TMR was highest in cows receiving C, intermediate in EG and DG, and lowest in RT at 26.9, 23.6, 24.7 and 20.3 kg DM/day respectively. Pasture intake was similar in cows receiving EG or DG, but was higher in RT at 2.4, 2.0 and 3.5 kg DM/day respectively. Milk yield was similar between cows receiving C, EG or DG, but lowest in RT at 45.7, 44.2, 44.9 and 41.7 kg/cow, respectively, while milk fat content of C18:3 n-3 was increased by grazing. Cows in C spent more than 55 min/day longer lying and had three additional lying bouts/day, while lying bouts were shorter than for cows receiving EG, RT or DG. It is concluded that high-yielding cows can be grazed for 6 hr/day with little impact on performance, provided TMR is available ad libitum when housed.  相似文献   

Evaluation of an HMGA2 variant for pleiotropic effects on height and metabolic traits in ponies     

Elaine M. Norton  Felipe Avila  Nichol E. Schultz  James R. Mickelson  Ray J. Geor  Molly E. McCue 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(2):942-952

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Field Evaluation of Cocksfoot,Tall Fescue and Phalaris for Dry Marginal Environments of South‐Eastern Australia. 1. Establishment and Herbage Production          下载免费PDF全文

S. G. Clark  Z. N. Nie  R. A. Culvenor  C. A. Harris  R. C. Hayes  G. D. Li  M. R. Norton  D. L. Partington 《Journal of Agronomy and Crop Science》2016,202(2):96-114

Newly developed candidate cultivars of cocksfoot (Dactylis glomerata L.), phalaris (Phalaris aquatica L.) and tall fescue (Festuca arundinacea Schreb. = syn. L. arundinaceum (Schreb) Darbysh.) were evaluated over four years for persistence and productivity against current commercial cultivars in small plots at five locations selected for lower and less reliable rainfall and difficult soils (low pH and high Al) in south‐eastern Australia known to be marginally too dry for these grass species. The five locations were ‐ representing summer dominant rainfall, Inverell, in northern New South Wales (NSW); ‐ representing uniform rainfall; Trungley Hall, (medium rainfall), and Beckom (lower rainfall) both in southern NSW; and; representing a winter dominant pattern ‐ Eversley, (higher rainfall), and Bealiba, (lower rainfall), in central Victoria. The objective was to determine if the new candidate cultivars were more likely to persist and to be productive than current commercial cultivars. The study showed that most phalaris and cocksfoot treatments were highly productive in high rainfall years at one or both sites in southern NSW. However, all treatments had become much less productive by the end of the experimental period due to plant loss under hot, dry conditions in the final summer. At Bealiba in central Victoria, cocksfoot was the most productive species with several cocksfoot treatments of both subsp. hispanica and subsp. glomerata still present at the final harvest despite a hot and dry final summer‐autumn. Tall fescue was the most productive species in the two higher rainfall environments (Inverell, Eversley) although most treatments of all species performed well at those sites. Continental tall fescues were more productive on average than Mediterranean tall fescues at the strongly acidic Eversley site. As a result of this work, two of the new cocksfoot candidates (Moroccan Fine and AVH48 Selection) and one of the tall fescue candidates (Summer Active 1) have been licensed for commercial development and release.  相似文献