Serum 5'nucleotidase activity in rats: a method for automated analysis and criteria for interpretation     

Carakostas MC  Power RJ  Banerjee AK 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1990,19(4):109-113

A manual kit for determining serum 5'nucleotidase (5'NT, EC 3.1.3.5) activity was adapted for use with rat samples on a large discrete clinical chemistry analyzer. The precision of the method was good (within-run C.V. = 2.14%; between-run C.V. = 5.5%). A comparison of the new automated method with a manual and semi-automated method gave regression statistics of y = 1.18X -3.66 (Sy. x = 4.54), and y = 0.733X + 1.97 (Sy. x = 1.69), respectively. Temperature conversion factors provided by the kit manufacturer for human samples were determined to be inaccurate for converting results from rat samples. Analysis of components contributing to normal variation in rat serum 5'NT activity showed age and sex to be major factors. Increased serum 5'NT activity was observed in female rats when compared to male rats beginning at about 5 to 6 weeks of age. An analysis of variance of serum 5'NT, alkaline phosphatase, and GGT activities observed over a 9-week period in normal rats suggests several advantages for 5'NT as a predictor of biliary lesions in rats.  相似文献   

Finding best exotic breed proportion in crossbred lactating sheep kept under farmers’ conditions in Ethiopia determined by use of nested Legendre polynomials with limited data     

W. G. Haile  S. Banerjee  A. Ayele  T. Mestawet  G. Klemetsdal  T. Ådnøy 《Acta Agriculturae Scandinavica, Section A - Animal Sciences》2018,68(4):174-180

ABSTRACT

The present study was conducted to estimate milk production performance and fit lactation curves for groups of ewes of Local and of Awassi crosses, with a variable blood level, reared under farmer’s environment. The Weigh-Suckle-Weigh method plus hand milking was used to estimate milk yield for ewes. A total of 466 observations from 115 ewes were used. Estimated least-squares adjusted means for the milk production over 120 days were 0.56?kg day^?1 (Local), 0.67 (<30% Awassi), 0.86 (30–50% Awassi), and 0.96 (>50% Awassi). Groups with 30–50% Awassi and >50% Awassi ewes produced significantly (p?<?0.05) more milk than Local ewes. Significant differences were observed between <30% Awassi and >50% Awassi crossbred groups. The best crosses (>50% Awassi) produced over 70% more milk than the local ewes which demonstrates the potential that exists in increasing milk production through the initiated crossbreeding programme with sheep in Ethiopia.  相似文献   

A potentized homeopathic drug, Arsenicum Album 200, can ameliorate genotoxicity induced by repeated injections of arsenic trioxide in mice     

Banerjee P  Biswas SJ  Belon P  Khuda-Bukhsh AR 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2007,54(7):370-376

Groundwater arsenic contamination has become a menacing global problem. No drug is available until now to combat chronic arsenic poisoning. To examine if a potentized homeopathic remedy, Arsenicum Album-200, can effectively combat chronic arsenic toxicity induced by repeated injections of Arsenic trioxide in mice, the following experimental design was adopted. Mice (Mus musculus) were injected subcutaneously with 0.016% arsenic trioxide at the rate of 1 ml/100 g body weight, at an interval of 7 days until they were killed at day 30, 60, 90 or 120 and were divided into three groups: (i) one receiving a daily dose of Arsenicum Album-200 through oral administration, (ii) one receiving the same dose of diluted succussed alcohol (Alcohol-200) and (iii) another receiving neither drug, nor succussed alcohol. The remedy or the placebo, as the case may be, was fed from the next day onwards after injection until the day before the next injection, and the cycle was repeated until the mice were killed. Two other control groups were also maintained: one receiving only normal diet, and the other receiving normal diet and succussed alcohol. Several toxicity assays, such as cytogenetical (chromosome aberrations, micronuclei, mitotic index, sperm head anomaly) and biochemical (acid and alkaline phosphatases, lipid peroxidation), were periodically made. Compared with controls, the drug fed mice showed reduced toxicity at statistically significant levels in respect of all the parameters studied, thereby indicating protective potentials of the homeopathic drug against chronic arsenic poisoning.  相似文献   

Effects of salinity on the growth and proximate composition of selected tropical marine periphytic diatoms and cyanobacteria     

Helena Khatoon  Sanjoy Banerjee  Fatimah Md Yusoff  Mohamed Shariff 《Aquaculture Research》2010,41(9):1348-1355

Marine periphytic cyanobacteria and diatoms have been examined as a potential source of feed supplement for rearing aquatic larvae in the aquaculture industry. Culture of the periphytic diatom Amphora sp., Navicula sp., Cymbella sp. and the cyanobacteria Oscillatoria sp. at different salinities showed significant changes in biomass and specific growth rates. Diatoms growth was significantly higher at 35 g L⁻¹, while for cyanobacteria growth was better at 25 g L⁻¹. Significantly higher levels of protein and lipid were found in diatoms at low salinities (15–25 g L⁻¹) and an increase in carbohydrate at high salinities (30–35 g L⁻¹). Conversely, cyanobacteria showed a significantly higher lipid content at 30–35 g L⁻¹ compared with other salinity levels but no significant changes were observed in the protein and carbohydrate contents at different salinity levels. The present findings can be taken into consideration when culturing marine periphytic Amphora sp., Navicula sp., Cymbella sp. and Oscillatoria sp. to provide appropriate levels of protein, lipid and carbohydrate as feed supplement as well as for bioremediation in aquaculture.  相似文献   

Photostabilization of quinalphos by crystal violet on the surface of kaolinite and palygorskite     

Kaushik Banerjee  Prem Dureja 《Pest management science》1995,43(4):333-337

Photochemical stability of the insecticide, quinalphos (O,O-diethyl O-quinoxalin-2-yl phosphorothioate), adsorbed on kaolinite and palygorskite was studied. In all cases considerable photostabilization was achieved in comparison with the sample of pesticide in its free form. Improvement of photostabilization was achieved when a cationic dye (crystal violet) was co-adsorbed with the quinalphos. The interactions between quinalphos and clays were studied by Fouriertransform infra-red spectroscopy. It is suggested that photostabilization is due to steric hindrance imposed by the clay surface to the isomerization step of photochemical reaction and pacification of clay surface by crystal violet.  相似文献   

Resistance to early blight of tomato with respect to various parameters of disease epidemics   总被引：3，自引：1，他引：2  

Koshlendra Kumar?PandeyEmail author  Padma Kant?Pandey  Gautam?Kalloo  Malay Kumar?Banerjee 《Journal of General Plant Pathology》2003,69(6):364-371

Resistance to early blight in the tomato was assessed by examining various parameters of the progress of the disease. Artificial inoculation and the scoring technique were standardized. Test plants were inoculated with 125cfu/ml of a 12-day-old culture of a pathogenic isolate of Alternaria solani. Screening under artificial conditions was more informative than that under natural epidemic conditions. Tomato cultivars CLN-2071-C, CLN-2070-A, BSS-174, and DTH-7 with resistance expressed as slow blighting against four pathogenic isolates of A. solani, were selected for cultivation in disease-prone areas. Disease intensity increased with the age of plants under the same inoculum load. The area under the disease progress curve (AUDPC) was positively correlated with the percentage disease index and negatively with resistance. Calculation of the apparent infection rate (r) was more informative for natural epidemics than for artificial conditions. The sequential apparent infection rate between observation periods was better correlated with disease progress than was the total apparent infection rate between the first and last observations. A double sigmoidal disease progress curve during the same cropping season was characteristic of some varieties when fungal infection took place during the vegetative phase of crop growth.  相似文献   

Identification of foot-and-mouth disease from a captive kangaroo in a zoological garden in India     

Bhattacharya S  Banerjee R  Ghosh R  Biswas A  Chatterjee A 《The Veterinary record》2003,153(16):504-505

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Immunization of cattle with nymphal Hyalomma anatolicum anatolicum extracts: effects on tick biology     

Sangwan  A.K.  Banerjee  D.P.  Sangwan  N. 《Tropical animal health and production》1998,30(2):97-106

Antigens derived from partially engorged nymphs of Hyalomma anatolicum anatolicum were used in immunizing crossbred (Bos indicus×Bos taurus) cattle against larval, nymphal and adult H. a. anatolicum and H. dromedarii. The cattle were either infected with Theileria annulata at low parasitaemia or were uninfected. Whole nymphal extract (WNE), nymphal membrane antigens (NMA) and nymphal soluble antigens (NSA) were used for immunization. The group immunized with WNE showed significant and better rejection of H. a. anatolicum ticks as compared to calves immunized with either NMA or NSA. The moulting rates of both engorged larvae and nymphs remained unaffected. Nymphs which engorged on the immunized calves were fully susceptible to infection by T. annulata as indicated by the intensity and abundance of Theileria infections in the resulting adult ticks from immunized and unimmunized Theileria infected cattle. These ticks also transmitted fatal theileriosis to susceptible calves.  相似文献   

Colchicine treatment of different varieties of potatoes in West Bengal     

H. C. Choudhuri  S. Banerjee 《Potato Research》1961,4(2):144-151

Summary Colchicine treatment of sprouted tubers before planting increased yield in 17 out of the 20 varieties reported. In some varieties this effect was maintained in the two succeeding years in which the tubers from the various treatments were grown, although no further treatment had been given. The optimum duration of treatment varied between varieties as did overall response. Treatment depressed yield in a few varieties, while in others yield increase was delayed. In 8 out of the 20 varieties abnormalities in tuber shape occurred. Treatment had no effect on maturation except in a few cases where a retardation of 2 or 3 days was recorded.

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Zusammenfassung Die Colchicin-Behandlung von vorgekeimten Knollen steigerte den Ernteetrag bei 17 der angeführten 20 Sorten. Bei manchen Sorten blieb diese Wirkung durch zwei aufeinanderfolgende Jahre in welchen die Knollen nach den verschiedenen Behandlungen gezogen wurden, erhalten, obwohl keine weitere Behandlung angewandt wurde. Die optimale Dauer der Behandlung war je nach den Sorten verschieden, ebenso das allgemeine Ansprechen auf die Behandlungen. Bei wenigen Sorten führte die Behandlung zu einer Ertragsdepression, w?hrenddem bei anderen die Ertragserh?hung verz?gert wurde. Bei 8 der 20 Sorten kamen Abnormit?ten der Knollenform vor. Die Behandlung hatte keine Wirkung auf die Reife ausser in einigen F?llen in welchen eine Versp?tung von 2 bis 3 Tagen verzeichnet wurde.

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Résumé Le traitement de tubercules germés à la colchicine avant la plantation a fait augmenter le rendement de 17 des 20 variétés étudiées. Dans certaines variétés, cet effet se manifesta pendant les deux années successives où les tubercules soumis aux différents traitements furent cultivés, bien que nul autre traitement ne f?t appliqué. La durée optimale du traitement variait d'une variété, à l'autre, de même que la réponse générale au traitement. Le traitement réduisit le rendement de quelques variétés, tandis que dans d'autres cas, l'augmentation du rendement se manifesta avec un certain retard. Dans 8 variétés sur 20, il fut constaté des anomalies de conformation des tubercules. Le traitement n'influen?ait pas la maturation, sauf dans quelques cas où il fut observé un retard de 2 ou 3 jours.

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First report of Calonectria cerciana causing leaf blight of Eucalyptus in northern India     

Ratnaboli Bose  Shailesh Pandey  Pooja Joshi  Sabyasachi Banerjee  Amit Pandey  Maneesh S. Bhandari 《Forest Pathology》2021,51(1):e12658

Eucalyptus spp. and their hybrids are frequently cloned and mass planted across farmland tracts and commercial plantations in northern India. It is a viable feeder species to the paper and pulp industries in this region. In 2018 and 2019, during field surveys conducted in northern India, a serious leaf blight disease was frequently observed in E. tereticornis plantations. Isolation from the blighted leaf samples consistently yielded fungal isolates having Calonectria‐like morphology. Morphological features coupled with sequence analysis of partial β‐tubulin (TUB2) and partial translation elongation factor‐alpha (TEF1) gene regions of two fungal isolates confirmed the species as Ca. cerciana. In detached leaf assays and glasshouse inoculation experiments, both isolates produced symptoms similar to those observed on the naturally infected leaves. Koch's postulates were fulfilled by re‐isolating Ca. cerciana from the inoculated leaves. This work is the first to confirm that Ca. cerciana is associated with a serious leaf blight disease of Eucalyptus in northern India and is an important addition to the taxonomy of Calonectria fungi in India.  相似文献