Cryopreservation of Piau‐Breed Wild Boar Sperm: Assessment of Cooling Curves and Centrifugation Regimes          下载免费PDF全文

HH Shiomi  RO Pinho  DMA Lima  JB Siqueira  MCR Santos  EV Costa  PS Lopes  SEF Guimarães  JD Guimarães 《Reproduction in domestic animals》2015,50(4):545-553

This study aimed to assess the effects of different cooling curves and centrifugation regimes used in cryopreservation protocols on the post‐thaw viability of Piau‐breed wild boar (Sus scrofa) sperm using in vitro assessment tests. Two centrifugations (800  g for 10 min and 2400  g for 3 min) and two cooling curves (conventional cooling using nitrogen vapour – freezing 1 and automated cooling using a programmed freezing machine – freezing 2) were tested. Therefore, the treatments were divided into M3 – centrifugation at 2400  g for 3 min and freezing 2; M10 – centrifugation at 800  g for 10 min and freezing 2; R3 – centrifugation at 2400  g for 3 min and freezing 1; and R10 – centrifugation at 800  g for 10 min and freezing 1. No significant differences (p > 0.05) between treatments occurred post‐thawing regarding the total sperm motility means recorded. The mean values of the different treatments were not different from each other regarding the supravital staining (SV), hypo‐osmotic test (HO), sperm–egg binding assay or sperm morphology. This study showed that both the cooling curve and the centrifugation regime affected the quality of post‐thaw sperm, and centrifugation for shorter times and cooling curves using automated cooling are the most suitable for minimizing sperm injury.  相似文献   

Comparison of two postoperative complication grading systems after treatment of stifle and shoulder instability in 68 dogs     

Faolain M. Barrett BVM&S  Jason A. Bleedorn DVM MS  DACVS-SA  Kyle D. Hutcheson DVM  DACVS-SA  Bryan T. Torres DVM PhD  DACVS-SA  DACVSMR  Derek B. Fox DVM  PhD  DACVS 《Veterinary surgery : VS》2023,52(1):98-105

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Longitudinal tendon healing assessed with multi-modality advanced imaging and tissue analysis     

Sherry A. Johnson  Alejandro Valdés-Martínez  Philip J. Turk  Cyril Wayne McIlwraith  Myra F. Barrett  Kirk C. McGilvray  David D. Frisbie 《Equine veterinary journal》2022,54(4):766-781

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Effect of the Medium Replacement Interval on the Viability,Growth and In Vitro Maturation of Isolated Caprine and Ovine Pre‐Antral Follicles     

DM Magalhães  DD Fernandes  MBS Mororó  CMG Silva  GQ Rodrigues  JB Bruno  MHT Matos  CC Campello  JR Figueiredo 《Reproduction in domestic animals》2011,46(1):134-140

The aim of the present study was to evaluate the effects of different medium replacement intervals on the viability, antral cavity formation, growth and in vitro maturation (IVM) of oocytes from caprine and ovine pre‐antral follicles. Pre‐antral ovarian follicles (≥150 μm) were isolated from the ovarian cortex of goats and sheep and were individually cultured for 24 days using two different medium replacement intervals [2 days (T₁) or 6 days (T₂)]. Follicle development was evaluated on the basis of antral cavity formation, increases in follicular diameter and the presence of healthy cumulus oocyte complexes and fully grown oocytes. For caprine species, results showed a higher percentage (p < 0.05) of viable follicles in T₁ than T₂ from day 6 until the end of the culture. In addition, when comparing both treatments after the same culture duration, the rate of antrum formation was significantly higher in T₁ than in T₂ from day 12 onwards. Yet, in ovines, when both treatments were compared on day 24 of the culture, there were more viable follicles in T₂ than in T₁ (p < 0.05). In the caprine species, percentages of fully grown oocytes (≥110 μm) acceptable for IVM after 24 days of culture were significantly higher in normal follicles cultured in T₁ (30.0%) than in T₂ (6.7%; p < 0.05). On the other hand, in ovines, at the end of the culture, the percentage of oocytes destined for IVM was higher in T₂ than in T₁ (23.5% vs 2.9%; p < 0.05). In conclusion, under the same conditions, the frequency of medium replacement significantly affected the in vitro development of caprine and ovine pre‐antral follicles. To improve the efficiency of the culture system, the medium must be replaced every 2 and 6 days for goat and sheep pre‐antral follicles, respectively.  相似文献   

Soundscape in a context of acoustic and landscape ecology     

Barry Truax  Gary W. Barrett 《Landscape Ecology》2011,26(9):1201-1207

Soundscape ecology is being proposed as a new synthesis that leverages two important fields of study: landscape ecology and acoustic ecology. These fields have had a rich history. Running “in parallel” for over three decades now, soundscape ecology has the potential to unite these two (among other) fields in ways that provide new perspectives on the acoustics of landscapes. Each of us was involved in the “birth” of these two fields. We each reflect here on the rich history of landscape ecology and acoustic ecology and provide some thoughts on the future of soundscape ecology as a new perspective.  相似文献   

Modeling lumber strength spatial variation using trend removal and kriging analyses     

Frank Lam  J. D. Barrett 《Wood Science and Technology》1992,26(5):369-381

Summary The techniques of kriging and trend removal analysis for modeling the strength properties of lumber have been discussed. Using these techniques, the within member compressive strengths of 38 mm × 89 mm 2100f-1.8E and the within member tensile strengths of 38 mm × 89 mm No. 2 spruce-pine-fir lumber have been modeled. The suitability of these techniques to model the strength properties of lumber has been evaluated by comparing the kriged compressive and tensile strength data to baseline experimentally collected compressive strength data and simulated tensile strength data, respectively. Comparisons of the results of statistical analyses of the baseline data and the kriged data show good agreement. The use of kriging and trend removal techniques to generate strength values in simulation studies for finite element analyses of wood structures is judged to be effective.  相似文献   

Bending strength adjustments for moisture content for structural lumber     

Dr. J. D. Barrett  W. Lau 《Wood Science and Technology》1991,25(6):433-447

Summary Effects of moisture content on bending strength and section capacity have been studied assuming that strength properties are linearly related to moisture content below the fiber saturation point. The models developed using the linear strength vs. moisture content relationships are compared with quadratic models developed earlier. The linear representation of strength variation with moisture content leads to predicted member bending capacity relationships which show that increases in member bending strength with moisture content will compensate for section property decreases at all bending strength property levels. Comparisons of bending strength and bending capacity models show that the two modelling approaches lead to similar predicted member performance. Simplified procedures are provided for adjusting member bending strength for moisture content which are suitable for materials properties codes or standards.  相似文献   

Modified algorithm to determine localized modulus of elasticity of lumber     

F. Lam  Prof. Dr. R. O. Foschi  Prof. Dr. J. D. Barrett  Technician Q. Y. He 《Wood Science and Technology》1993,27(2):81-94

Summary A procedure has been developed to determine the cut-off frequency in an algorithm to evaluate the localized modulus of elasticity (MOE) in lumber. MOE profiles have been artificially generated to serve as input for evaluation of the procedure. A noise component has also been added to the input record to test the robustness and accuracy of the procedure. Good agreement between program predictions and the known localized MOE values has been obtained. The efficiency of the procedure has also been tested using MOE profiles collected from experiments. The program performed efficiently and program predictions seemed reasonable.  相似文献   

Soil carbon turnover in the McMurdo Dry Valleys, Antarctica     

J.E. Barrett  R.A. Virginia  D.H. Wall 《Soil biology & biochemistry》2006,38(10):3065-3082

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Terrestrial ecosystem processes of Victoria Land, Antarctica     

J.E. Barrett  R.A. Virginia  J. Aislabie  J.G. Bockheim  W.B. Lyons  J.N. Nkem  H. Steltzer  M.D. Wallenstein 《Soil biology & biochemistry》2006,38(10):3019-3034

Terrestrial environments of Victoria Land, Antarctica are ideal systems to test hypotheses about the sensitivity of ecosystem processes to climate variability, and the relationships between soil biodiversity and ecosystem functioning because of their high sensitivity to climate change and their limited diversity. This region is also considered among the most pristine of ecosystems, and therefore may serve as an indicator for detecting the response of other ecosystems to global environmental change. Rates and controls over key ecosystem processes remain poorly documented over much of Victoria Land, but it is generally held that the distribution and functioning of soil communities are most limited by the availability of liquid water and organic carbon. Here we review examples of ecosystem processes from several sites in North and South Victoria Land and develop a regional synthesis accounting for variation in the availability of soil resources (i.e. liquid water, organic matter, inorganic nutrients). Variation in soil microclimate, organic matter, moisture and salinity encountered over gradients of coastal to interior sites, latitude, and soil chronosequences are the primary controls over the structure of soil communities and their functioning. Imbalanced stoichiometric nutrient ratios frequently encountered in Victoria Land ecosystems also contribute to limited distribution of soil biota, and where they occur these elemental imbalances indicate lower biological activity and little biotic control over bulk element ratios in soils. Priorities and future directions of Victoria Land soil and ecosystem research are also discussed.  相似文献