Noninvasive monitoring of doxorubicin cardiotoxicity: a pilot study of two dogs     

K. A.  Selting  A. W. Spier  J. R. Dodam  J. W. Skimming  J. C. Lattimer  L. G. Britt  J. R. Turk  J. W. Tyler  H. E. Durham  M. Garro 《Veterinary and comparative oncology》2004,2(2):107-108

The cardiotoxicity of anthracycline anticancer agents, most notably doxorubicin, limits their use in treating a wide variety of cancers. Currently available methods for assessment of anthracycline‐induced cardiotoxicity (AIC) often lack specificity and sensitivity. The purpose of this pilot investigation was to determine noninvasive diagnostic methods that are predictive for AIC. Two mongrel dogs were anesthetized for injection of doxorubicin (22.5 mg) directly into the left coronary artery. Doxorubicin injection was repeated 2 weeks later. Dogs were followed for 12 weeks or until heart failure was documented. Dogs were monitored before and at serial time points after doxorubicin using echocardiography and signal‐averaged electrocardiography as well as ambulatory ECG monitoring. Blood was also collected for measurement of cardiac troponin I and T concentrations. Doxorubicin was readministered at 6 and 12 weeks after the second injection, as interim data analysis did not reveal adequate evidence of cardiomyopathy. Dog‐1 appeared to be more sensitive than Dog‐2 to AIC based upon gradual changes in variables of cardiac function and earlier time to failure (113 days). In contrast, Dog‐2 had inconsistent changes in cardiac function and a longer time to failure (139 days). Dog‐1 also had greater peak plasma cardiac troponin I levels (17.96 ng/ml) as compared to peak levels in Dog‐2 (4.08 ng/ml). In addition, Dog‐1 had increased ventricular extrasystolic contractions (VE)(0/day at baseline, 443/day one week prior to death), whereas dog‐2 showed very few VE's throughout. Spectral analysis of ECG data also revealed a significant decrease over time in total spectral power in Dog‐1 from 37,148 at baseline to 603 msec²/Hz one week prior to death, indicating decreased parasympathetic tone. Endomyocardial biopsies revealed minor changes despite significant clinical abnormalities. These data indicate differences in susceptibility to AIC between Dog‐1 and Dog‐2, and suggest a role for these noninvasive measures in monitoring the heart during chemotherapy.  相似文献   

A longitudinal study of oestrous characteristics and conception in tie-stalled and loose-housed Swedish dairy cows     

S. Nyman  S. E. Malm  H. Gustafsson  B. Berglund 《Acta Agriculturae Scandinavica, Section A - Animal Sciences》2016,66(3):135-144

The presence and importance of visual oestrous symptoms and the relation to conception, breed, housing system and milk yield were studied. Data were collected from a Swedish research herd during a 16-year period. Local, secondary and primary oestrous symptoms and an overall oestrus intensity score (OI) were studied. Decreasing trends in oestrous expression and conception rates (CR), and an increase in CR with more intensive oestrus, from 24% at OI score one (very week) to 54% at OI score five (very strong) were observed. The mean interval from first to last observed oestrus symptom was long with a large variation (54?±?SD?=?32?h). Generally, irrespectively of housing system, Swedish Red cows had higher CR, more intensive oestruses and longer oestrus durations compared to Swedish Holstein cows. Local oestrous symptoms, with CR of 24–28%, were most frequently detected, which is why more attention should be paid to local oestrous symptoms.  相似文献   

Diagnosing appendicular osteosarcoma with ultrasound-guided fine-needle aspiration: 36 cases     

Britt T  Clifford C  Barger A  Moroff S  Drobatz K  Thacher C  Davis G 《The Journal of small animal practice》2007,48(3):145-150

OBJECTIVES: To determine the ability to obtain diagnostic cytology samples from appendicular bone lesions using ultrasound-guided needle aspirations. Secondary objectives were to compare cytological evaluations with histopathological results and to determine the utility of staining malignant mesenchymal cells for the presence of alkaline phosphatase. METHODS: Aspirations from 36 aggressive appendicular bone lesions with histological diagnoses were included in the study. Ultrasound was used to guide the needle to the medullary cavity or the adjacent soft tissue mass. The smears stained with Wright-Giemsa and nitroblue tetrazolium chloride/5-bromo-4-chloro-3-indoyl phosphate toluidine salt (NBT/BCIP) were examined. RESULTS: A diagnostic sample was obtained in 32 of the 36 cases. Of the 32 diagnostic samples, cytology indicated sarcoma, with a sensitivity of 97 per cent (confidence interval: 83 to 100 per cent) and a specificity of 100 per cent (confidence interval: 16 to 100 per cent). When a diagnosis of sarcoma was made on cytology, alkaline phosphatase staining indicated osteosarcoma, with a sensitivity of 100 per cent (confidence interval: 87 to 100 per cent). CLINICAL SIGNIFICANCE: The results of this study indicate that ultrasound-guided needle aspiration of aggressive bone lesions is a viable technique for identifying malignant mesenchymal cells and for diagnosing sarcomas. It is cost-effective and minimally invasive. Furthermore, identifying alkaline-phosphatase-negative malignant mesenchymal cells from a bone aspiration may rule out osteosarcoma, whereas alkaline-phosphatase-positive malignant mesenchymal cells are suggestive of osteosarcoma.  相似文献   

Structured surfactant formulation for pesticides     

Jill E. Newton  John Sholl  Britt Pessala  John Caseley 《Pest management science》1993,37(2):208-209

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Use of pneumocystoplasty for overinflation of the swim bladder in a goldfish     

Britt T  Weisse C  Weber ES  Matzkin Z  Klide A 《Journal of the American Veterinary Medical Association》2002,221(5):690-3, 645

A Ryukin goldfish was evaluated because of a 6-month history of progressive abdominal distention and positive buoyancy. Overinflation of the swim bladder was diagnosed, and the fish was anesthetized with tricaine methanesulfonate. Archimedes' principle was used to determine the volume of swim bladder that was removed surgically. The caudal swim bladder was exteriorized through an abdominal incision and 2 surgical clips were placed across it to limit its size. After surgery, the fish remained in a state of negative buoyancy in sternal and lateral recumbency on the bottom of the tank. Sutures were removed 15 days after surgery, but the fish died 24 days after surgery. A full necropsy could not be performed because of autolysis of the tissues, but the surgical clips and the swim bladder appeared unremarkable. Pneumocystoplasty may be a viable treatment for this condition.  相似文献   

Evaluation of numbers of microscopic and macroscopic follicles in cattle selected for twinning     

Cushman RA  Hedgpeth VS  Echternkamp SE  Britt JH 《Journal of animal science》2000,78(6):1564-1567

We hypothesized that the number of microscopic follicles present in the ovaries of cattle selected for twin births (Twinner) would be greater than in the ovaries of contemporary Controls. Ovaries were collected from seven Control and seven Twinner cows at slaughter. The number of Small (1 to 3.9 mm), Medium (4 to 7.9), and Large (> 8 mm) surface follicles was counted and one ovary was fixed for histological evaluation. Fifty to sixty consecutive 6-microm slices were taken from a piece of cortical tissue, approximately 1 cm x 1 cm in area, located between the surface follicles. Microscopic follicles were classified as primordial (oocyte surrounded by a single layer of squamous pregranulosa cells), primary (oocyte surrounded by a single layer of one or more cuboidal granulosa cells), secondary (oocyte surrounded by two or more layers of granulosa cells), or tertiary (oocyte surrounded by multiple layers of granulosa cells with initiation of antrum formation to < or = 1 mm in diameter). The total number of follicles was counted in 200 fields (2 mm x 2 mm) per ovary. A field containing no follicles was classified as empty. There were significantly more secondary follicles in Twinner compared with Control ovaries (12.9 vs 6.3; P < .05). Twinners also tended to have more small surface follicles (35.4 vs 49.0; P < 0.1). We conclude that ovaries of Control and Twinner cows do not differ in the number of primordial follicles or in the number of follicles activated into the growing pool; however, Twinner cows are able to maintain more growing follicles at the secondary and subsequent stages of development.  相似文献   

Validation with biological markers for food intake of a dietary assessment method used by Swedish women with three different dietary preferences     

Johansson G  Akesson A  Berglund M  Nermell B  Vahter M 《Public health nutrition》1998,1(3):199-206

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FACIAL FIBROSARCOMA IN TWO COWS   总被引：1，自引：0，他引：1  

Lisa G.  Britt  DVM  MS  Ohn R.  Middleton  DVM  Reginlad A.  Valdez  DVM  MS  Russell L.  Tucker  DVM  Steven M.  Parish  DVM  Jeff W.  Tyler  DVM  PhD 《Veterinary radiology & ultrasound》1998,39(1):18-21

Fibrosarcoma is a common tumor affecting bone, but is uncommon in cows, Reported here are two bovine cases of facial fibrosarcoma that were very invasive.  相似文献   

Causes of death and illness in the native sheep of North Ronaldsay, Orkney. I. Adult sheep     

D P Britt  J R Baker 《The British veterinary journal》1990,146(2):129-142

Post-mortem examinations on 71 native sheep found dead on the island of North Ronaldsay, Orkney in four visits between April 1983 and July 1985 were carried out. The sheep in this almost feral flock have access to a small area of unmanaged moorland pasture but are otherwise restricted to the foreshore where they subsist largely on Laminaria spp. and other seaweeds. Young adult animals died largely of heavy parasite burdens combined with inadequate nutrition, while the older sheep often starved because of severe dental disease precipitated by heavy deposits of tartar on the cheek teeth--rarely seen in sheep on a more conventional diet. Other underlying metabolic conditions, such as the extensive mineralization of the kidney medulla in many mature sheep, may be debilitating. The pathological findings suggest that adaptation to the peculiar environmental rigours and dietary restrictions on North Ronaldsay is less complete than has previously been assumed.  相似文献   

Influence of footing surface on mounting and other sexual behaviors of estrual Holstein cows     

L D Vailes  J H Britt 《Journal of animal science》1990,68(8):2333-2339

Seven ovariectomized Holstein cows, treated sequentially with progesterone and estradiol benzoate to induce estrus, were used to determine whether cows showed a preference for mounting and displaying other sexual behaviors toward estrual or nonestrual cows tied on dirt or concrete surfaces. Preference tests were conducted in a test area that consisted of equal-sized concrete and dirt surfaces; two cows, designated A and B, were tied on the two surfaces, one on either side. Cows A and B were treated so that on each of four test days both were estrual, only A was estrual, only B was estrual or neither A nor B were estrual. On each test day, five estrual test cows were introduced individually into the test area for two 30-min test periods. During the first test period, tied cow A was on concrete and tied cow B was on dirt, but during the second test period, their positions were switched. Test cows were able to move freely from surface to surface and to interact with tied cow A or B. Estrual test cows spent 21.6 +/- 1.4 min of each 30-min test period on dirt, regardless of the estrual status of the cow tied on concrete or dirt. Mounting activity was 3- to 15-fold greater on dirt than on concrete when there was an estrual cow tied on dirt, regardless of the estrual status of the cow on concrete. Mounting activity was fourfold greater on concrete than on dirt when there was a tied estrual cow on concrete and a tied nonestrual cow on dirt.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献