Intracavitary Cisplatin Chemotherapy Experience with Six Dogs   总被引：1，自引：0，他引：1  

Antony S. Moore MVSc    Claudia Kirk DVM  Aglaia Cardona DVM 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1991,5(4):227-231

Six dogs with a median age of 7 years (range = 5-14 years) were presented for signs referable to thoracic or abdominal effusion associated with neoplasia of the body cavities. Intracavitary cisplatin was administered at 50 mg/m2 every 4 weeks for a median of 2.5 treatments (mean = 3, range = 1-6). Three dogs with pleural mesothelioma had complete resolution of effusion for 289, 129, and greater than 306 days without evidence of tumor growth. Resolution of effusion occurred after one treatment in two dogs and after two treatments in one dog. In three dogs with carcinomatosis of unknown origin, complete responses was seen in two dogs after one treatment for 255 and greater than 807 days, respectively. Intracavitary chemotherapy with cisplatin was associated with palliation and control of malignant pleural and/or abdominal effusion in five of six dogs. Toxicity was minimal, and this method of therapy should be further explored in dogs with similar malignancies.  相似文献   

Validation of a human immunoturbidimetric assay to measure canine albumin in urine and cerebrospinal fluid.     

Fabio Gentilini  Francesco Dondi  Cinzia Mastrorilli  Massimo Giunti  Claudia Calzolari  Gualtiero Gandini  Danilo Mancini  Paolo Famigli Bergamini 《Journal of veterinary diagnostic investigation》2005,17(2):179-183

The aim of this study was to validate an automated immunoturbidimetric assay used to quantify human albumin in urine and to accurately measure canine albumin concentrations in both urine and cerebrospinal fluid. The partial homology existing between human and canine albumin limited the accuracy of the human assays in measuring canine albumin without method modifications. Thus, the assay was modified by calibrating the analyzer with calibrators made in the laboratory containing known concentrations of canine albumin. To prepare the set of calibrators, the albumin concentration of pooled sera of healthy dogs was assessed in 5 replicates using the BromocresolGreen assay. Pooled samples were aliquoted and serially diluted to obtain the expected concentrations of albumin (0.5, 1, 5, 13, and 30 mg/dl) for establishing the canine calibration curve. Thereafter, the performance was assessed by analyzing canine urine and CSF The modified assay accurately quantified canine albumin in both specimens, as indicated by the following. Intra- and interassay variability was 0.92% and 2.74%, respectively; recovery was 99.66% and 99.07% in urine and 105.02% in CSF No interference was detected when hemolysate and glucose were added to urine. The test was linear within the verified range (0-225 mg/dl). These results demonstrate that the modified human albumin immunoturbidimetric assay can be a useful tool in the veterinary diagnostic laboratory. It is accurate and tends itself to automatization on chemistry analyzers.  相似文献   

Comparison between the urine dipstick and the pH‐meter to assess urine pH in sheep and dogs          下载免费PDF全文

Labrini V. Athanasiou  Panagiotis D. Katsoulos  Eleni G. Katsogiannou  Zoe S. Polizopoulou  Myrto Diamantaki  Constantinos Kamatsos  Georgios Christodoulopoulos 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2018,47(2):284-288

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Molecular detection of sugarcane striate virus and sugarcane white streak virus and their prevalence in the Miami World Collection of sugarcane and related grasses     

Wardatou Boukari  Denis Filloux  Jean Heinrich Daugrois  Emmanuel Fernandez  Dimitre Mollov  Claudia Kaye  Martha Hincapie  Alexander Sanchez  Jianping Wang  Philippe Roumagnac  Philippe Rott 《Plant pathology》2020,69(6):1060-1069

Viruses in the genus Mastrevirus (family Geminiviridae), including those infecting sugarcane, have natural geographical ranges almost exclusively restricted to Africa and the Indian Ocean islands off the African coast. Only sugarcane white streak virus (SWSV) in Barbados and sugarcane striate virus (SStrV) in Florida and Guadeloupe are known to infect a few sugarcane varieties in the Western Hemisphere. In this study, PCR assays were developed to detect these two viruses in sugarcane. Five hundred and seventy-one DNA samples from Saccharum species and interspecific hybrids from the Miami World Collection of sugarcane and related grasses were tested for the presence of SStrV and SWSV by PCR. No variety was found infected by SWSV but SStrV was detected in 19 varieties. PCR data were confirmed by sequencing amplified fragments (248 bp). These fragments shared 93%–100% nucleotide identity with SStrV sequences from the GenBank database. SStrV isolates were distributed in six phylogenetic groups, including the four strains of the virus. Most varieties infected by SStrV originated from Asia, thus confirming a previous hypothesis stating that this virus originated from this continent. Absence of SStrV in commercial sugarcane in Florida also suggested that this virus has not been spread in this location, while infected plants have been present for several decades.  相似文献   

Participatory mapping of landscape values in a Pan-European perspective     

Maria Garcia-Martin  Nora Fagerholm  Claudia Bieling  Dimitris Gounaridis  Thanasis Kizos  Anu Printsmann  Matthias Müller  Juraj Lieskovský  Tobias Plieninger 《Landscape Ecology》2017,32(11):2133-2150

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α-Transducin and α-gustducin immunoreactive cells in the stomach of common sole (Solea solea) fed with mussel meal     

Maurizio Mazzoni  Alessio Bonaldo  Pier Paolo Gatta  Claudia Vallorani  Rocco Latorre  Marco Canova  Paolo Clavenzani 《Fish physiology and biochemistry》2015,41(3):603-612

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The influence of the choice of preemptive analgesia on long‐term postsurgical pain after tibial plateau leveling osteotomy in dogs     

William Pownall  Ulrich Rytz  Gertraud Schuepbach  Claudia Spadavecchia  Helene Rohrbach 《Veterinary surgery : VS》2021,50(1):71-80

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Quantitative sensory testing of the equine face     

Kata O. Veres-Nyéki  József Nyéki  Gábor Bodó  Claudia Spadavecchia 《Equine veterinary journal》2021,53(1):177-185

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Developmental competence of heat stressed oocytes from Holstein and Limousine cows matured in vitro     

Konstantina Stamperna  Eleni Dovolou  Themistoklis Giannoulis  Maria Kalemkeridou  Ioannis Nanas  Katerina Dadouli  Katerina Moutou  Zissis Mamuris  Georgios S. Amiridis 《Reproduction in domestic animals》2021,56(10):1302-1314

The negative effects of heat stress on dairy cattle's fertility have been extensively studied, but the relevant knowledge for beef cattle is rather limited. The aims of this study were to investigate the effects of HS during in vitro maturation on the developmental potential of oocytes derived from Limousine and Holstein cows and to estimate the effect of the differential gene expression of important genes in oocytes, cumulus cells and blastocysts in the growth competence between the breeds. In seven replicates, cumulus oocyte complexes from Holstein and Limousine cows were matured for 24 hr at 39°C (controls C; Hol_39, Lim_39) or at 41°C from hour 2 to hour 8 of IVM (treated T; Hol_41, Lim_41), fertilized, and presumptive zygotes were cultured for 9 days at 39°C. Cleavage and embryo formation rates were evaluated 48 hr post-insemination and on days 7, 8 and 9, respectively. From all groups, subsets of cumulus cells, oocytes and blastocysts were analysed for the relative expression of genes related to metabolism, stress, apoptosis and placentation. No difference was detected in cleavage rate or in blastocyst formation rate among the control groups. In both breeds, heat stress reduced blastocyst yield, but at all days the suppression was higher in Limousines. In Holsteins, altered gene expression was detected in cumulus cells (G6PD, GLUT1) and blastocysts (PLAC8), while in Limousines, differences were found in oocytes (G6PD, HSP90AA1), in cumulus cells (CPT1B, HSP90AA1, SOD2) and blastocysts (DNMT, HSP90AA1, SOD2). It appears that Holstein COCs are more tolerant than Limousine COCs, possibly due to compulsory, production driven selection.  相似文献   

Ultrasonographic findings of the corpus luteum and the gravid uterus during heat stress in dairy cattle     

Ioannis Nanas  Mariana Barbagianni  Katerina Dadouli  Eleni Dovolou  Georgios S Amiridis 《Reproduction in domestic animals》2021,56(10):1329-1341

The objectives of this study were to assess alterations in, echogenic appearance, size and blood flow in the corpus luteum, the placentomes and the blood flow in umbilical and uterine arteries that heat stress can cause in cooled pregnant dairy cows. Pregnant cows were allocated in two groups and the gravid uteri, along with the ipsilateral corpora lutea were examined during the winter (group W, n = 9) or the summer (group S, n = 10). The grey-scale ultrasound and colour flow imaging of the corpus luteum and placentome were performed. In addition, the umbilical and uterine artery diameters and haemodynamic parameters in the vessels were calculated. At the time of ultrasonographic examination, cortisol concentrations were higher, and progesterone levels tended to be lower in group S compared to group W. The grey-scale ultrasound evaluation of corpora lutea and placentomes was lower in group S compared to group W. The diameter of umbilical artery and the blood volume in the vessel were less in group S than in group W. We infer that heat stress affects foetal blood supply and possibly the structure of placentomes and corpora lutea, but it differently affects the blood flow characteristics in the umbilical and uterine arteries.  相似文献